1_alignment.ppt

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1

2 " " "

3 " n " n n " n

4

5

6 " n n n " n n n n

7 " n LGPSSKQTGKGW-SRIWDN! + +! LN-ITKSAGKGAIMRLGDA! " n TGKG !! AGKG !

8 " n w w w " n w w

9 " " "

10

11 11

12 12

13

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15

16 " n w w w " n w w

17 A! M! O! A! I! M! S! S! AIM-S! A-MOS!

18 A! I! M! S! A! M! O! S!

19 ! A I M S! A M O S!

20 A T A T G C C T T! ! A ! A ! G ! C ! C ! T ! G ! T !

21 ATATGCCT-T! A-A-GCCTGT! A T A T G C C T T! ! A ! A ! G ! C ! C ! T ! G ! T !

22 ATGCCT-T! AAGCCTGT! A T A T G C C T T! ! A ! A ! G ! C ! C ! T ! G ! T !

23

24 Ala 1! Arg -5 7! Asn ! Asp ! Cys ! Gln ! Glu ! Gly ! His ! Ile ! Leu ! Lys ! Met ! Phe ! Pro ! Ser ! Thr ! Trp ! Tyr ! Val ! A R N D C Q E G H I L K M F P S T W Y V!

25 " n n n n w w n n w

26 " n n n w w n

27 " n n " n n n n n

28

29 " Woese, C. R., Kandler, O. and Wheelis, M. L. (1990) Towards a natural system of organisms: Proposal for the domains, Archaea, Bacteria and Eukarya. Proc. Natl. Sci. USA, 87, " Gibbs, A. J. and McIntyre, G. A. (1970) The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences. Eur. J. Biochem., 16, " Needleman, S. B. and Wunsch, C. D. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol., 48, " Smith, T. F. and Waterman, M. S. (1981) Identification of common molecular subsequences. J. Mol. Biol., 147, " Gotoh, O. (1982) An improved algorithm for matching biological sequences. J. Mol. Biol., 162, " Dayhoff, M. O., Schwartz, R. M. and Orcutt, B. C. (1978) A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure, Vol. 5, Suppl. 3, pp " Henikoff, S. and Henikoff, J. G. (1992) Amino acid substitution matrices from protein blocks. Proc. Natl. Acad. Sci. USA, 89,

30 " n n " n n " n n " n n

31 31

32 " n n " n n n n " n n n

33 " n n n n " n

34 プロテオーム インタラクトーム解析と バイオインフォマティクス " プロテオーム n SWISS-2DPAGE 2次元電気泳動 質量分析 " インタラクトーム n n n n n 相互作用している 結合し ている タンパク質のペア を網羅的に調べる 酵母2ハイブリッド 質量分析 タンパク質チップ インタラクトームとも言う " 類似発現タンパク質のクラス " タリング タンパク質相互作用ネット ワーク構築 34

35 " " " " " "

36 " " n n " n n