ドージンニュース_134号.indd
|
|
- いおり たもん
- 5 years ago
- Views:
Transcription
1 Generation of Artifi cial Enzymes in Directed Evolution. Advances in methods for conformational prediction, structural analysis and site-directed mutagenesis of proteins and peptides have contributed to the understanding of their structure and function. However, with the exception of a few successes, the generation of practical functional molecules solely by rational design remains a difficult challenge. The aim of our study is to investigate molecular design relying on evolutionary processes, called as directed evolution, to generate a novel class of biofunctional molecules. This evolutionary approach consists of three steps; 1) constructions of protein/peptide libraries based on structural information, 2) expressions of the libraries on phage particles, and 3) selections with investigator-imposed selective pressures. In this work, we study on generation of artificial 20 DNA DNA DNA DNA biocatalysts by immune system that uses the evolutionary processes to give receptor-like molecules. As a natural enzyme binds the transition state of the chemical reaction to lower the activation energy, immunization with a putative transition-state analog (TSA), with the expectation that the induced antigencombining site could be both geometrically and electronically complementary to the transition-state, provides catalytic antibodies. We have succeeded to generate antibodies catalyzing highly stereo and regio-specific hydrolyses with immunization of phosphonate transition-state analogs. Furthermore, to evolve catalytic antibodies toward higher catalytic activity, phage-displayed antibody (Fab) libraries were constructed to screen antibodies with optimized differential affinity for the transition state relative to the ground state. Recently, we have demonstrated a new strategy for generating catalytic antibodies, namely, by the generation of antigen-combining site that function as an apoprotein for binding functionalized components, called holoabzyme. Replacement of the functionalized components enables a single antibody to catalyze multiple reactions, -elimination, decarboxylation and aldol reactions. Catalytic antibodies have great potential for generating novel catalysts as well as for providing opportunities to examine the evolutionary dynamics of enzymes. 1) 2) 3) 4) 5) 6) 7) 2 1 H V H, D, J H Lκ Vκ, Jκ V H 100 D 1
2 1 15 J H 5 Vκ Jκ 5 B B 10 8 B 1 B 2 3 B 3 1 1, (S ) (P) (S) (S ) (DG uncat ) E) (S ) (DG TS ) (DG cat ) (S) DGS (DGcat) (K TS ) (K m ) K m /K TS = k cat /k uncat 2
3 3 A A B (1) (2) 4 11 (2) (3) KLH 12 (2) 6 6D , 11 ph (3) C10 K TSA = μm k cat = min -1 6D9 1 (min -1 ) (min -1 M -1 ) 3
4 5 k cat = min -1 K TSA = μm 5A (k cat /k uncat ) K m /K TSA 6D9 8D11 4B5 9C10, 3G6 5 k cat /k uncat K m /K TSA 5B 6D9 X 13) 6D9 6 2 Tyr (H58) Trp (H100i) 6 6D9 X Fab L :,H ;, His(L27d) His(L27d) Ala 14) 6D9 L CDR-1 His(L27d) 15, 16) B , 19,
5 4.2 6D9 19, X 6D9 L CDR1 His L27d 6 13,14) His (6D9) (8Hg) His (L27d) Ser(L27e) Tyr ( 6) His (L27d) His (L27d) Tyr L27e 2 Tyr L27e Ser Ser (AGT) Tyr (TAT, TAC) D9L CDR1 2 L CDR1 5
6 8 5.1 (5) (6) (7) 22) (6) 86% 5.2 (10) (8) - (9) 4) (11) (12) 5) L- D
7 9 ( 9) 7) (13) 4 4 (14) Michaelis- Menten 4 k cat = min -1, K m = 6.6 μm, k cat / k uncat = 2700 (15) ) Holoabzyme 6.1 B B 11 (16) (16) 2 p- N- 2 (17) (18) (19) (20) - (23) (24) - (19) ( 11) 6.2 (16) KLHBSA KLH- (KLH-1) Balb/c (16) (16) (17) (18) 22 2 (25E2, 27C1) (18) (19) (20) (18) 198 (25E2) 109 (27C1) (19) (25E2) (27C1) (20) (25E2) (27C1) (16) 7
8 NHAc AcHN X AcHN 2N P H 18:X=H 19:X=NH 2 20:X=SH 24 AcHN b- 2N 17 18,19,20 X 21:X=0,22:X=NH 2N H 2N F N 25 2N H 19 2N H 2N 19 2N H (25E2: K d = 27 nm; 27C1: K d = 8.7 nm) ( - ) (16) 6.3 (24) - 25E2 - (23) (25) - 27C1 - K m 24 = 122 μm, K m 23 = 864 μm, k cat = 0.89 min -1 k cat /K m 23/k uncat = C-C I (19) I I I - (19) I - (28) 11 27C1 K m 19 = 6.9 mm, 8
9 K m 28 = 2.1 mm, k cat = 1.29 min -1 k cat /K m 28/ k uncat = E2, 27C REFERENCES 1) Schultz P. G., Lerner R. A., Science, 1995,269, ),,1999, 35,695. 3) Teraishi K., Saito M., Fujii I., Nakarura H., Tetrahedron Lett.,1992, 33, ) Fujii I., Lerner R. A., Janda K. D., J. Am. Chem. Soc.,1991,113, ) Tanaka F., Kinishita K., Tanimura R., Fujii I., J. Am. Chem. Soc.,1996,118, ) Tanaka F., da M., Fujii I., Teterahedron Lett., 1998, 39, ) Iwabuchi Y., Miyashita H., Tanimura R., Kinoshita K., Kikuchi M.,Fujii I.,J. Am. Chem. Soc., 1994,116,771. 8) Iwabuchi Y., Kurihara S., da M., Fujii I., Teterahedron Lett.,1999, 40, ) Kurihara S., Tsumuraya T., Suzuki K., Kuroda M., Liu L., Takaoka Y., and Fujii I., Chem. Eur. J., 2000,6, ) Kakinuma H., Fujii I., Nishi Y., J. Immu. Method,2002, 269, ) Miyashita H., Karaki Y., Kikuchi M., Fujii I., Proc. Natl. Acac. Sci. USA, 1993, 90, ) Fujii I., Tanaka F., Miyashita H., Tanimura R., and Kinoshita K., J. Am. Chem. Soc., 1995,117, ) Kristensen1., Vassylyev D. G., Tanaka F., Morikawa K., Fujii I., J. Mol. Biol., 1998, 281, ) Miyashita H., Hara T., Tanimura R., Fujii I., J. Mol. Biol., 1997, 267, ) da M., Ito N., Tsumuraya T., Suzuki K., Sakakura M., and Fujii I., J. Mol. Bi ol., 2007, 369, ) Tsumuraya T. and Fujii I., Bull. CHem. Soc. Jpn., 2008, 81, ) Hoogenboom H. R., Trends Biotechnol., 1997,15, ) Fujii I., Fukuyama S., Iwabuchi Y., Tanimura R., Nat. Biotechnol., 1998,16, ) Takahashi N., Kakinuma H., Liu L., Nishi Y., Fujii I., Nat. Biotechnol., 2001, 19, ) Takahashi-Ando N., Kakinuma H., Fujii I., Nishi Y., Journal of Immunological Methods, 2004, 294, 1. 21),,,2009, ) Ikeda S., Weinhouse M., Janda K. D., Lerner R. A., Danishefsky S. J., J. Am. Chem. Soc., 1991,113, ) Ishikawa F., Tsumuraya T., Fujii I., J. Am. Chem. Soc., 2009,131, (Ikuo Fujii) Tel: Fax: fujii@b.s.osakafu-u.ac.jp HP: () ( )
DojinNews_Vol.134_#699B44.eps
Review 1 5 10 Topics on Chemistry Bicyclic peptides 12 14 C16-PEG-SAMs Amino-EG 6 -hexadecanethiol, hydrochloride A505 10 mg 45,000 Carboxy-EG 6 -hexadecanethiol C463 10 mg 32,000 Hydroxy-EG 6 -hexadecanethiol
More information4) H. Takayama et al.: J. Med. Chem., 45, 1949 (2002). 5) H. Takayama et al.: J. Am. Chem. Soc., 112, 8635 (2000). 6) H. Takayama et al.: Tetrahedron Lett., 42, 2995 (2001). 9) M. Kitajima et al: Chem.
More informationyakugaku-kot.ppt
2009 Masaaki Kotera kot@kuicr.kyoto-u.ac.jp 2 I II / A () B1 () B2 B12 C () D A D () () () () DNA 5- http://www.genome.jp/kegg/pathway.html KEGG PATHWAY Database Xenobiotics biodegradation http://www.genome.jp/kegg/pathway.html
More information1_alignment.ppt
" " " " n " n n " n " n n n " n n n n " n LGPSSKQTGKGW-SRIWDN! + +! LN-ITKSAGKGAIMRLGDA! " n -------TGKG--------!! -------AGKG--------! " n w w w " n w w " " " 11 12 " n w w w " n w w A! M! O! A!
More informationuntitled
E-mail: khatano@fms.saitama-u.ac.jp Tel & Fax: 048-858-3535 Toxin Virus Bacterium Glycolipid Glycoprotein Vero toxin (Stx1 and Stx2) Side view Bottom view H H H H N H Grobotriaosyl Ceramide (Gb 3 : Galα1-4Galβ1-4Glcβ-Cer)
More informationCorrelation between molecular evolution of P450s and evolution of plant secondary metabolism Masaharu Mizutani E-mail : mizutani@scl.kyoto-u.ac.jp URL : http://biofun.kuicr.kyoto-u.ac.jp/index-j.html 13)
More informationIR0036_62-3.indb
62 3 2016 253 272 1921 25 : 27 8 19 : 28 6 3 1921 25 1921 25 1952 27 1954 291960 35 1921 25 Ⅰ 0 5 1 5 10 14 21 25 34 36 59 61 6 8 9 11 12 16 1921 25 4 8 1 5 254 62 3 2016 1 1938.8 1926 30 1938.6.23 1939.9
More information46 12 3 1 ATP ( ) ATP ~P 1~P hyd Gº 1 1 1950 ~P hyd Gº Hansia et al. Biophys Chem 119: 127, 2006 ~P hyd Gº 1? ATP hyd Gº ATP ATP ATP ~P ε ~P George [BBA 223: 1, 1970] ~P 2) hyd Gº 1. ph Mg 2+ 2. 1 2 2
More information光学
Control of Refractive Indices of Optical Polymers: Theoretical Prediction and Molecular Design Method Shinji ANDO The fundamental theory and a method to predict the refractive indices and dispersions of
More informationNetsu Sokutei 19 (4) Thermal Transitions and Stability of Fatty Acid-Containing and Defatted Bovine Serum Albumin (BSA) Michiko Kodama, Shinji
Netsu Sokutei 19 (4) 163-169 Thermal Transitions and Stability of Fatty Acid-Containing and Defatted Bovine Serum Albumin (BSA) Michiko Kodama, Shinji Takebayashi, Shun-ichi Kidokoro* and Hatsuho Uedaira**
More information10中西_他.indd
Streptomyces mobaraensis N N N Sm b Sm N N Sm Sm Sm ' ' Sm Sm ak K k K bk k K Sm Sm a b S. mobaraensis a b a b Sm b b bb b b Sm Sm S. lividans ab Sm ab Sm ab ab ab ab SmSm S. lividansab S. lividans ab
More information日本看護管理学会誌15-2
The Journal of the Japan Academy of Nursing Administration and Policies Vol. 15, No. 2, PP 135-146, 2011 Differences between Expectations and Experiences of Experienced Nurses Entering a New Work Environment
More informationï\éÜA4*
Feature Article Imaging of minuscule amounts of chemicals, Scannimg Chemical Microscope --- Increasing analysis information through imaging --- Abstract We have developed a Scanning Chemical Microscope
More informationLaser Ablation Dynamics of Amorphous Film of a Cu-Phthalocyanine Derivative Masahiro HOSODA*,**, Hiroshi FURUTANI*,**. Hiroshi FUKUMURA*,** Hiroshi MASUHARA*, Masanobu NISHII*** Nobuyuki ICHINOSE**,***,
More information表1.eps
Vol.1 C ontents 1 2 cell-free EMBO J Proc. Natl. Acad. Sci. USA NatureEMBO J 3 RNA NatureEMBO J Nature EMBO J 4 5 HCV HCV HCV HCV RNA HCV in situ 6 7 8 Nat Struct Mol Biol J Biol Chem Nat Commun J Virol
More information第62巻 第1号 平成24年4月/石こうを用いた木材ペレット
Bulletin of Japan Association for Fire Science and Engineering Vol. 62. No. 1 (2012) Development of Two-Dimensional Simple Simulation Model and Evaluation of Discharge Ability for Water Discharge of Firefighting
More information01_梅村佳代_紀要_2007最終
Bull. Nara Univ. Educ., Vol., No. (Cult. & Soc.), An Historical Study of Education in the Tajima Province, from the Late Early-Modern Times until the Early Meiji Era UMEMURA Kayo (Department of School
More informationuntitled
SUMMARY This paper presents current situations, issues, and several practical use scenarios of anonymous communication which provides sender anonymity and receiver anonymity in a communication. As methods
More information<95DB8C9288E397C389C88A E696E6462>
2011 Vol.60 No.2 p.138 147 Performance of the Japanese long-term care benefit: An International comparison based on OECD health data Mie MORIKAWA[1] Takako TSUTSUI[2] [1]National Institute of Public Health,
More informationThe function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Database Center for Life Science Online Service Key words Shigey
The function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Key words Shigeyuki Kawai, Chikako Fukuda, Kousaku Murata E-mail : kawais@kais.kyoto-u.ac.jp, chikakko@kais.kyotou.ac.jp,
More informationOJT Planned Happenstance
OJT Planned Happenstance G H J K L M N O P Q R . %. %. %. %. %. %. %. %. %. %. %. %. %. %. %. %. %. %. .... Q ......... . Planned Happenstance.. pp.- VOL.,NO. pp., Current Status of Ritsumeikan Employees
More information2797 4 5 6 7 2. 2.1 COM COM 4) 5) COM COM 3 4) 5) 2 2.2 COM COM 6) 7) 10) COM Bonanza 6) Bonanza 6 10 20 Hearts COM 7) 10) 52 4 3 Hearts 3 2,000 4,000
Vol. 50 No. 12 2796 2806 (Dec. 2009) 1 1, 2 COM TCG COM TCG COM TCG Strategy-acquisition System for Video Trading Card Game Nobuto Fujii 1 and Haruhiro Katayose 1, 2 Behavior and strategy of computers
More informationEVALUATION OF NOCTURNAL PENILE TUMESCENCE (NPT) IN THE DIFFERENTIAL DIAGNOSIS OF IMPOTENCE Masaharu Aoki, Yoshiaki Kumamoto, Kazutomi Mohri and Kazunori Ohno Department of Urology, Sapporo Medical College
More information< > Introduction to Basic Physical Chemistry 1,2 2 [ advanced [ [ [ [ [ KULASIS
< > Introduction to Basic Organic Chemistry 1,2 [ 2 [ 10 11 12 13 14 15 [ [ [ [ KULASIS < > Introduction to Basic Physical Chemistry 1,2 2 [ advanced [ 11 12 14 15 [ [ [ [ KULASIS < > Introduction to Basic
More informationTHE INSTITUTE OF ELECTRONICS, INFORMATION AND COMMUNICATION ENGINEERS TECHNICAL REPORT OF IEICE.
THE INSTITUTE OF ELECTRONICS, INFORMATION AND COMMUNICATION ENGINEERS TECHNICAL REPORT OF IEICE. E-mail: {ytamura,takai,tkato,tm}@vision.kuee.kyoto-u.ac.jp Abstract Current Wave Pattern Analysis for Anomaly
More information<30315F836D815B83675F95D08BCB8E812E696E6464>
Sociological Essays 7 : Anticipating changes in an opaque society Shinji KATAGIRI Abstract After the Great East Japan Earthquake, it became very difficult to predict the future of Japanese society. However,
More informationuntitled
11-19 2012 1 2 3 30 2 Key words acupuncture insulated needle cervical sympathetick trunk thermography blood flow of the nasal skin Received September 12, 2011; Accepted November 1, 2011 I 1 2 1954 3 564-0034
More informationJFE.dvi
,, Department of Civil Engineering, Chuo University Kasuga 1-13-27, Bunkyo-ku, Tokyo 112 8551, JAPAN E-mail : atsu1005@kc.chuo-u.ac.jp E-mail : kawa@civil.chuo-u.ac.jp SATO KOGYO CO., LTD. 12-20, Nihonbashi-Honcho
More informationタンパク質を物性科学的に見た場合、単なるアミド結合の集合体であると片づけるにはもったいなさすぎる
Precise Functional Analysis of Biological Macromolecules Promoted by the Encounter of Crystallographic and Spectroscopic Molecular Structure Analyses Takamitsu Kohzuma*, Sachiko Yanagisawa, and Tomotake
More information大学における原価計算教育の現状と課題
1 1.1 1.2 1.3 2 2.1 2.2 3 3.1 3.2 3.3 2014a 50 ABC Activity Based Costing LCC Lifecycle Costing MFCA Material Flow Cost Accounting 2 2 2016 9 1 2 3 2014b 2005 2014b 2000 1 2 1962 5 1 3 2 3 4 5 50 2012
More informationIPSJ SIG Technical Report Secret Tap Secret Tap Secret Flick 1 An Examination of Icon-based User Authentication Method Using Flick Input for
1 2 3 3 1 Secret Tap Secret Tap Secret Flick 1 An Examination of Icon-based User Authentication Method Using Flick Input for Mobile Terminals Kaoru Wasai 1 Fumio Sugai 2 Yosihiro Kita 3 Mi RangPark 3 Naonobu
More informationJournal of the Combustion Society of Japan Vol.58 No.185 (2016) ORIGINAL PAPER 火災旋風近傍の流れに関する研究 Flow Around a Fire Whirl *
58 185 2016 167-171 Journal of the Combustion Society of Japan Vol.58 No.185 (2016) 167-171 ORIGINAL PAPER 火災旋風近傍の流れに関する研究 Flow Around a Fire Whirl * ONISHI, Hiroyuki and KUWANA, Kazunori 992-8510 4-3-16
More information橡自動車~1.PDF
CIRJE-J-34 2000 10 Abstract In this paper, we examine the diversity of transaction patterns observed between a single pair of one automaker and one auto parts supplier in Japan. Assumed reasonably that
More informationWATOC2005 Best Poster Diamond Certificate α -(BEDT-TTF) 2 IBr 2 H.turkmenica X H C H O Brønsted Al K Electronic structure of CeNiGe 2 x Si x studied by Ce 4d-4f resonant and angleresolved photoemission
More informationKey Words: probabilisic scenario earthquake, active fault data, Great Hanshin earthquake, low frequency-high impact earthquake motion, seismic hazard map 3) Cornell, C. A.: Engineering Seismic
More informationODA NGO NGO JICA JICA NGO JICA JBIC SCP
ODA NGO NGO JICA JICA NGO JICA JBIC SCP - - NGO NGO NGO NGO NGO NGO Roger A Hart - Potuvil UGM UGM APU NGO APU APU NGO APU NGO NGO APU APU Matara NGO ODA NGO ODA http://www.jica.go.jp/partner/college/index.html#partnership
More informationseparation encounter initiation fulfillment return PR CM FAX J DA S J Nicholson, Nigel (1990) The transition cycle: Causes, outcomes, processes and forms In Shirley Fisher and Cary L. Cooper
More information1 Fig. 1 Extraction of motion,.,,, 4,,, 3., 1, 2. 2.,. CHLAC,. 2.1,. (256 ).,., CHLAC. CHLAC, HLAC. 2.3 (HLAC ) r,.,. HLAC. N. 2 HLAC Fig. 2
CHLAC 1 2 3 3,. (CHLAC), 1).,.,, CHLAC,.,. Suspicious Behavior Detection based on CHLAC Method Hideaki Imanishi, 1 Toyohiro Hayashi, 2 Shuichi Enokida 3 and Toshiaki Ejima 3 We have proposed a method for
More information28_3-03-伊勢坊 中原先生-原著③.indd
Japanese Journal of Administrative Science Volume 28, No.3, 2016, 233-247 Article How do executive secretaries learn from their experiences? Aya ISEBO Tokyo University Jun NAKAHARA Tokyo University The
More informationKey words : Adverse reactions, Egg allergy, IgG antibody, Mills allergy, FAST
Key words : Adverse reactions, Egg allergy, IgG antibody, Mills allergy, FAST 13) Danaeus, A., Johansson, S. G. O., Foucard, T. & Ohman, S.: Clinical and immunogical aspects of food allergy in childhood.
More information1 2 8 24 32 44 48 49 50 SEC journal Vol.11 No.2 Sep. 2015 1 2 SEC journal Vol.11 No.2 Sep. 2015 SEC journal Vol.11 No.2 Sep. 2015 3 4 SEC journal Vol.11 No.2 Sep. 2015 SEC journal Vol.11 No.2 Sep. 2015
More informationBull. of Nippon Sport Sci. Univ. 47 (1) Devising musical expression in teaching methods for elementary music An attempt at shared teaching
Bull. of Nippon Sport Sci. Univ. 47 (1) 45 70 2017 Devising musical expression in teaching methods for elementary music An attempt at shared teaching materials for singing and arrangements for piano accompaniment
More information1) K. J. Laidler, "Reaction Kinetics", Vol. II, Pergamon Press, New York (1963) Chap. 1 ; P. G. Ashmore, "Catalysis and Inhibition of Chemical Reactio
1) K. J. Laidler, "Reaction Kinetics", Vol. II, Pergamon Press, New York (1963) Chap. 1 ; P. G. Ashmore, "Catalysis and Inhibition of Chemical Reactions", Butterworths, London (1963) Chap. 7, p. 185. 2)
More information16_.....E...._.I.v2006
55 1 18 Bull. Nara Univ. Educ., Vol. 55, No.1 (Cult. & Soc.), 2006 165 2002 * 18 Collaboration Between a School Athletic Club and a Community Sports Club A Case Study of SOLESTRELLA NARA 2002 Rie TAKAMURA
More information<8ED089EF8B49977634342D312D30914F95742E696E6464>
The Treatments in the Institutions Regarded As Inappropriate by Certified Student Social Workers and Their Coping Behavior: Survey and Analysis Nobuko SAKATA (1) 15 13 (2) 47 16 (3) 53 44-1 2006 17 1810
More information研究成果報告書(基金分)
The most remarkable characteristic of the mammalian neocortex is its layered structure. Because all, but only the mammalian species, have this layered structure in the telencephalon, it has been accepted
More informationMicrosoft Word - toyoshima-deim2011.doc
DEIM Forum 2011 E9-4 252-0882 5322 252-0882 5322 E-mail: t09651yt, sashiori, kiyoki @sfc.keio.ac.jp CBIR A Meaning Recognition System for Sign-Logo by Color-Shape-Based Similarity Computations for Images
More information006.…G…l…‰…M†[(™|›º)P78†`
78 Energy and Environmental Consciousness Differences between Advanced and Developing Countries Abstract The purpose of the present study is to understand how much differences there are between advanced
More informationThe Indirect Support to Faculty Advisers of die Individual Learning Support System for Underachieving Student The Indirect Support to Faculty Advisers of the Individual Learning Support System for Underachieving
More information474 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No.3,.1..2* (,**0) 24 Measurement of Deterioration of Frying Oil Using Electrical Properties Yoshio
474 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. / No.3.1..2 (0) 24 Measurement of Deterioration of Frying Oil Using Electrical Properties Yoshio Hagura Yoshihiro Sasaki and Kanichi Suzuki Graduate School
More information1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The Boston Public Schools system, BPS (Deferred Acceptance system, DA) (Top Trading Cycles system, TTC) cf. [13] [
Vol.2, No.x, April 2015, pp.xx-xx ISSN xxxx-xxxx 2015 4 30 2015 5 25 253-8550 1100 Tel 0467-53-2111( ) Fax 0467-54-3734 http://www.bunkyo.ac.jp/faculty/business/ 1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The
More informationFA
29 28 15 1985 1993 The process of the labor negotiations of the Japan Professional Baseball Players Association, 1985 1993 ABE Takeru Graduate School of Social Science, Hitotsubashi University Abstract
More information< > Introduction to Basic Physical Chemistry 1,2 2 [ advanced [ [ [ [ [ KULASIS
< > Introduction to Basic Organic Chemistry 1,2 [ 2 [ 10 11 12 13 14 15 [ [ [ [ KULASIS < > Introduction to Basic Physical Chemistry 1,2 2 [ advanced [ 11 12 14 15 [ [ [ [ KULASIS < > Introduction to Basic
More informationδδ 1 2 δ δ δ δ μ H 2.1 C 2.5 N 3.0 O 3.5 Cl 3.0 S μ
1961. (Received January 15, 2013) 1) 1. 2. ph 2) 3) 2) 2) 2001 4 6) 7) ph ph ph 1961 1962 1. 2 1 1 δδ 1 2 δ δ δ δ μ 1 1 1 1. H 2.1 C 2.5 N 3.0 O 3.5 Cl 3.0 S 2.5 1 1. μ 1 3. 1 2. 1963 1,4- δ δ 1 3 2 δ
More informationThe Nutrient Intake of Young Women With Special Attention on the Trace Nutrients Including Folate SUZUKI Kazu', HIGASHINE Yuuko", ITOU Ryoko YAMADA Yukiko', KOSEKI Sakiyo*, OKUDA Toyoko"" Seibo Jogakuin
More information揃 24 1681 0 20 40 60 80 100 0 21 42 63 84 Lag [hour] Lag [day] 35
Forecasting Model for Electricity Consumption in Residential House Based on Time Series Analysis * ** *** Shuhei Kondo Nobayasi Masamori Shuichi Hokoi ( 2015 7 3 2015 12 11 ) After the experience of electric
More informationCore Ethics Vol. -
Core Ethics Vol. - Core Ethics Vol. - Core Ethics Vol. % NICU - Core Ethics Vol. - - - - Core Ethics Vol. - NICU NICU NICU NICU http://www.arsvi.com/d/i.htm -. http://www.normanet.ne.jp/~ww/,,.,,, -.
More information8y4...l
3 607 67 1 2 3 20 17 68 4 17 17 16101695 5 6 69 1645 1653 8 3 1663 5 7 70 8 9 10 71 1662 1010 13 11 72 12 13 73 1678 16611722 14250 70 90 14 168071 15 1685 16 74 168980 2 17 18 19 20 21 75 22 4 1658 23
More informationTable 1 Type of polymeric coating materials Fig. 2 Results of suppressive effects of polymeric coating materials on the progress of neutralization of concrete. Table 2 Evaluation of the suppressive effects
More information) 5 Lawton QOL 4 4 QOL Lawton 4) Philadelphia Geriatric Center Affect Rating ScaleARS ARS QOL 5) HDS R
Vol. 12 No. 2 2002 389 397 Λ1 Λ2 16 10 30 1 2 Lawton Philadelphia Geriatric Center Affect Rating ScaleARS Lawton ARS + ARS 6 2 ARS 2 12:00 15:0017:00 11:30 16:00 1 2 ARS 1 2 ARS QOL QOL QOL 1) QOL QOL
More informationブラジル産ジョロウグモから単離された新規アシルポリアミンの構造決定
J. School Sci. Eng. Kinki Univ. 41 (2005) 23-29 Structural Determination of Novel Acylpolyamines from the Venom of Nephila clavipes, a Brazilian Joro spider Takahiro NISHIMARU*, Terumi NAKAJIMA * *, Yasuhiro
More informationuntitled
ORYZA POLYAMINE ORYZA POLYAMINE -P -PC -LC 1. Ver.1.0 MM ver. 1.0MM 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504
More information(a) 1 (b) 3. Gilbert Pernicka[2] Treibitz Schechner[3] Narasimhan [4] Kim [5] Nayar [6] [7][8][9] 2. X X X [10] [11] L L t L s L = L t + L s
1 1 1, Extraction of Transmitted Light using Parallel High-frequency Illumination Kenichiro Tanaka 1 Yasuhiro Mukaigawa 1 Yasushi Yagi 1 Abstract: We propose a new sharpening method of transmitted scene
More informationX X 1. 1 X 2 X 195 3, 4 Ungár modified Williamson-Hall/Warren-Averbach 5-7 modified modified Rietveld Convolutional Multiple Whole Profile CMWP 8 CMWP
X X a a b b c Characterization of dislocation evolution during work hardening of stainless steels by using XRD line-profile analysis Tomoaki KATO a, Shigeo SATO a, Yoichi SAITO b, Hidekazu TODOROKI b and
More information04-01_論文(荻野基行)new.indd
1 1 Bulletin of Tokyo University and Graduate School of Social Welfare p83-93 (2010, 5) 372-0831 2020-1 2010 5 6 2 1 1 1959 1979 1965 1968 1987 2005 83 1 1955 p10 714 7 1954 p338 1717 1794 1992 p232-233
More informationIPSJ SIG Technical Report An Evaluation Method for the Degree of Strain of an Action Scene Mao Kuroda, 1 Takeshi Takai 1 and Takashi Matsuyama 1
1 1 1 An Evaluation Method for the Degree of of an Action Scene Mao Kuroda, 1 Takeshi Takai 1 and Takashi Matsuyama 1 The purpose of our research is to investigate structure of an action scene scientifically.
More information9 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.0,,/+,/0 (,**/) 251 * * E#ects of Microbial Transglutaminase on Melting Point and Gel property of G
9 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.0,,/+,/0 (,**/) 251 * * E#ects of Microbial Transglutaminase on Melting Point and Gel property of Gelatins Takahiko Soeda, Tomoko Kaneko*, Akiko Hokazono,
More informationCA HP,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,,.,,,,,.,,,,,,.,,,,,.,,,,,.,,,,,,.,,,,,,.,,
Ritsumeikan Alumni Program CA HP,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,.,,,,,,.,,,,,.,,,,,,.,,,,,.,,,,,.,,,,,,.,,,,,,.,,,,,.,,,,,. ,,, :,, :,,,
More information304 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.0, -*. -*3 (,**1) 58 * ** *** : * : ** *** Development of Sorting System Based on Potato Starch C
304 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.0, -*. -*3 (,**1) 58 * ** *** : * : ** *** Development of Sorting System Based on Potato Starch Content Using Visible and Near-Infrared Spectroscopy
More information04_奥田順也.indd
82016 pp. 45~58 STUDIES IN ART 8 Bulletin of Tamagawa University, College of Arts 2016 This study aimed to identify the contemporary issues considered necessary in today s keyboard harmonica instruction,
More information<30375F97E996D88E812E696E6464>
Abstract: This study is intended as an investigation of the transition of Lady Windermere s Fan on stage in the Republic of china. Oscar Wild s Lady Windermere s Fan was adapted for the Chinese stage by
More information<95AA8E718CA4838C835E815B D862E696E6464>
T1r T1r1 T1r3 T1r2 T1r3 [1] T1r T1r 500 LBD 1 LBD T1rLBD [2] T1r T1r2a T1r3 LBD [3] T1r2a/T1r3 L- [4] T1r2a/T1r3LBD M~ M T1rLBD FRET FRET T1rLBD [3] 1 T1r 2 T1r2a/T1r3 X [3] 28 75 March 2017 T1rLBD imsaxst1r2a/t1r3lbd
More information,,.,,.,..,.,,,.,, Aldous,.,,.,,.,,, NPO,,.,,,,,,.,,,,.,,,,..,,,,.,
J. of Population Problems. pp.,.,,,.,,..,,..,,,,.,.,,...,.,,..,.,,,. ,,.,,.,..,.,,,.,, Aldous,.,,.,,.,,, NPO,,.,,,,,,.,,,,.,,,,..,,,,., ,,.,,..,,.,.,.,,,,,.,.,.,,,. European Labour Force Survey,,.,,,,,,,
More information11号02/百々瀬.indd
Vol. 112011 ピア エデュケーションによる栄養学科学生の栄養教育の実践 Nutrition Education by College Students of Nutrition Science through the Peer Education System 百々瀬いづみ IzumiMOMOSE 山部秀子 Shuko YAMABE A ºpeer education" system has
More information25 6 7 X (crystallizing ligand) Fv Fv 3.5 Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound
More informationQRNL10-1.pm
42 2 Vol. 10 No.2, 2003 2003 2 1 5 Vol. 10 No. 2 2003 2 5 6 7 April 1, 2003 INQUA 8 9 11 11 2003 2003 2 2003 12003 8 29 9 1 2 2003 6 10 3 4 9 1 1 5 100 6. http://www.sci.osaka-cu.ac.jp/geos/ geo3/qr2003/index.htm
More informationCaloric Behavior of Chemical Oscillation Reactions Shuko Fujieda (Received December 16, 1996) Chemical oscillation behavior of Belousov- Zhabotinskii
Caloric Behavior of Chemical Oscillation Reactions Shuko Fujieda (Received December 16, 1996) Chemical oscillation behavior of Belousov- Zhabotinskii (BZ) and Briggs- Rauscher (BR) reactions was followed
More informationtikeya[at]shoin.ac.jp The Function of Quotation Form -tte as Sentence-final Particle Tomoko IKEYA Kobe Shoin Women s University Institute of Linguisti
tikeya[at]shoin.ac.jp The Function of Quotation Form -tte as Sentence-final Particle Tomoko IKEYA Kobe Shoin Women s University Institute of Linguistic Sciences Abstract 1. emphasis 2. Speaker s impressions
More informationWikiWeb Wiki Web Wiki 2. Wiki 1 STAR WARS [3] Wiki Wiki Wiki 2 3 Wiki 5W1H 3 2.1 Wiki Web 2.2 5W1H 5W1H 5W1H 5W1H 5W1H 5W1H 5W1H 2.3 Wiki 2015 Informa
情 報 処 理 学 会 インタラクション 2015 IPSJ Interaction 2015 A17 2015/3/5 Web 1 1 1 Web Web Position and Time based Summary System using Story Style for Web Contents Daichi Ariyama 1 Daichi Ando 1 Shinichi Kasahara
More informationJuntendo Medical Journal
* Department of Health Science Health Sociology Section, Juntendo University School of Health and Sports Science, Chiba, Japan (WHO: Ottawa Charter for Health promotion, 1986.) (WHO: Bangkok Charter
More information施 ほか/3-18
March 201 A Study on the Generating Mechanism and Preventive Measures of Communication Errors in Industrial Organizations Guirong Shi Shiichiro Inoue In the causative analyses of the accident and trouble
More informationCHEMOTHERAPY Fig. 1 Chemical structure of TE-031
CHEMOTHERAPY Fig. 1 Chemical structure of TE-031 CHEMOTHERAPY Table 1 Experimental groups for active systemic anaphylaxis in guinea pigs : Single dosage Fig. 2 Schedule for active systemic anaphylaxis
More information2 The Bulletin of Meiji University of Integrative Medicine 3, Yamashita 10 11
1-122013 1 2 1 2 20 2,000 2009 12 1 2 1,362 68.1 2009 1 1 9.5 1 2.2 3.6 0.82.9 1.0 0.2 2 4 3 1 2 4 3 Key words acupuncture and moxibustion Treatment with acupuncture, moxibustion and Anma-Massage-Shiatsu
More informationThe Japanese Journal of Experimental Social Psychology. 2003, Vol. 42, No. 2, 146-165 Discoursive Strategy for the Commitment to Adoption with Infants Who Had Been Reared at Residential
More informationA Study of the Comprehensive Framework of Action for Job Retention amongst the Deaf and Hard of Hearing Makoto IWAYAMA Abstract The purpose of this pa
Title 聴覚障害者の職場定着に向けた取り組みの包括的枠組みに関する考察 Author(s) 岩山, 誠 Citation 地域政策科学研究, 10: 1-24 Issue Date 2013-03-23 URL http://hdl.handle.net/10232/16661 http://ir.kagoshima-u.ac.jp A Study of the Comprehensive Framework
More information05_藤田先生_責
This report shows innovation of competency of our faculty of social welfare. The aim of evaluation competency is improvement in the Social welfare education effects, by understanding of studentʼs development
More information1) Y. Kobuke, K. Hanji, K. Horiguchi, M. Asada, Y. Nakayama, J. Furukawa, J. Am. Chem. Soc., 98, 7414(1976). 2) S. Yoshida, S. Hayano, J. Memb. Sci.,
1) Y. Kobuke, K. Hanji, K. Horiguchi, M. Asada, Y. Nakayama, J. Furukawa, J. Am. Chem. Soc., 98, 7414(1976). 2) S. Yoshida, S. Hayano, J. Memb. Sci., 11, 157(1982). 3) J. D. Lamb, J. J. Christensen, J.
More informationHuman Welfare 8‐1☆/4.坂口
1 2 1914 2007 2002 2013 2004 2013 2009 2011 5 1 2 Human Welfare 8 1 2016 1 110 2014 9 11 11 8 110 4 106 3 2.8 103 97.2 18 76 37.4 SD 16.5 2 1 1 3 2 10 65 2006 65 25 4 5 1 5 3 98 4 60 60 4 1 4 1 60 15.1
More information1
5-3 Photonic Antennas and its Application to Radio-over-Fiber Wireless Communication Systems LI Keren, MATSUI Toshiaki, and IZUTSU Masayuki In this paper, we presented our recent works on development of
More informationPDCA
PDCA / / -- -- -- -- -- -- % % --- --- - No.--- --- --- A B C D + + + + + + + + + A B C D........................ --- OJT PDCA Eliminate Combine ECRS Rearrange Simplify -- - BKC IT BKC BKC APU -- :
More information1) Topley & Wilsons: Principles of Bacterio- 583-591.-5) Huddleson, I.F.: Brucellosis in man and animals, 1943. -6) Wilson, G. S. & encountered in certain Br. abortus agglutinating sera, J. Path. & Bact.,
More information% 95% 2002, 2004, Dunkel 1986, p.100 1
Blended Learning 要 旨 / Moodle Blended Learning Moodle キーワード:Blended Learning Moodle 1 2008 Moodle e Blended Learning 2009.. 1994 2005 1 2 93% 95% 2002, 2004, 2011 2011 1 Dunkel 1986, p.100 1 Blended Learning
More information, 3, STUDY ON IMPORTANCE OF OPTIMIZED GRID STRUCTURE IN GENERAL COORDINATE SYSTEM 1 2 Hiroyasu YASUDA and Tsuyoshi HOSHINO
, 3, 2012 9 STUDY ON IMPORTANCE OF OPTIMIZED GRID STRUCTURE IN GENERAL COORDINATE SYSTEM 1 2 Hiroyasu YASUDA and Tsuyoshi HOSHINO 1 950-2181 2 8050 2 950-2181 2 8050 Numerical computation of river flows
More informationレーザ誘起蛍光法( LIF法) によるピストンの油膜挙動の解析
Analysis of Piston Oil Film Behavior by Using Laser Induced Fluorescence Method Shuzou Sanda, Akinori Saito ( Laser Induced Fluorescence Method LIF ) LIF Scanning -LIF Abstract Analysis of the oil film
More informationI Jpn Prosthodont Soc 47: 779-786, 2003 A Study of Masticatory Ability in the Elderly A Comparison between Dentulous Subjects and Denture - Wearers- Nakashima Mihoko, Okimoto Kimie, Matsuo Koichi and Terada
More information創薬と結晶構造解析-3.key
( 460-377 3 2 3 ? Neuraminidase and Tamiflu http://www.roche.com/research_and_development/innovation_and_technologies.htm 2? ( ) Ligand-based drug design Ligand-based drug design (Pharmacophore-based drug
More information学位研究17号
1715 3 The Student Transfer and the Articulation System in Chinese Higher Education HUANG Meiying Research in Academic Degrees, No. 17 March, 2003the article The Journal on Academic Degrees of National
More information1: ( 1) 3 : 1 2 4
RippleDesk Using Ripples to Represent Conversational Noise on Internet Shigaku Iwabuchi Takaomi Hisamatsu Shin Takahashi Buntarou Shizuki Kazuo Misue Jiro Tanaka Department of Comupter Science, University
More information06_学術_関節単純X線画像における_1c_梅木様.indd
Arts and Sciences X The formulation of femoral heard measurement corrected enlargement ratio using hip joints X-ray Imaging 1 2 1 1 1 2 Key words: Bipolar Hip Arthroplasty (BHA) Preoperative planning Enlargement
More information