3 1 Amersham Imager 680 RGB 94 Amersham Imager 680 QC 94 LED LED OD OD Amersham Imager 680 UV 94 Amersham Imager ImageQuant LAS LED LED

3 92 93 94 98 99 101 Ettan 102 104 106 108 109 110 111 102 103 / 107 / 112 Amersham Imager 680 4,739,200 94 ImageQuant LAS 500 2,573,000 98 Amersham Typhoon scanner RI 10,375,800 99 Melanie 971,500 102 ImageQuant TL IQTL 70,000 103 Ettan IPGphor 3 IEF System 1,364,000 110 SE 260 Mighty Small II Mini-Vertical Units 160,500 110 SE 600 Ruby Standard Vertical Units 353,300 110 minive Vertical Electrophoresis System 185,300 111 Amersham ECL Semidry Blotters 248,900 111 118,500 111

3 1 Amersham Imager 680 RGB 94 Amersham Imager 680 QC 94 LED LED OD OD Amersham Imager 680 UV 94 Amersham Imager 680 94 ImageQuant LAS 500 98 LED LED LED UV 1 312 nm 312 nm 312 nm 1 460 nm LED 520 nm LED 630 nm LED Cy2: 525BP20 Cy3/EtBr: 605BP40 Cy5: 705BP40 460 nm LED 365 nm UV LED Cy3/EtBr: 605BP40 Cy3/EtBr: 605BP40 560 LP 320 2048 1536 830 F 0.85(FUJINON)/ 43 mm F1.4/30 mm W D H 22 16 cm 11 8 cm 10.5 10.5 cm 360 485 785 mm 300 280 570 mm 43.6 kg 43.2 kg 42.7 kg 40.1 kg 15 kg 4.8 4.8 2 ImageQuant TL 1 2 ImageQuant TL Amersham Imager 680 / 600 UV / 680 QC 92 www.gelifesciences.co.jp

LAS-3000 2015 9 30 LAS-3000 mini 2015 9 30 LED Silver, CBB, X-ray, film, NBT, BCIP 3 LED 312 nm 460 nm LED 3 520 nm LED 3 630 nm LED 3 365 nm LED 710 nm LED EtBr: 605BP40 3 SYBR Green : Y515 3 : 525DF20 3 : R670 3 IR785 GFP 510DF10 UV L41 320 2048 1472 F 0.85(FUJINON)/43 mm 14 21 cm : 25 25 cm LED LED UV 460 nm LED EtBr: 605BP40 SYBR Green : Y515 320 2048 1472 F 0.85(FUJINON)/43 mm 12 18 cm 510 480 900 mm 440 380 750 mm 60 kg 40 kg 4.8 4.8 312 nm UV EtBr 365 nm UV LED EtBr SYPRO Rose Qdot 605/655/705/800 460 nm LED Alexa Fluor 488 Cy2 SYBR Green I / II SYBR Gold SYPRO Ruby SYPRO Orange FITC FAM AttoPhos Pro-Q Emerald 488 520 nm LED SYPRO Red Cy3 TAMRA 5-ROX HEX Alexa Fluor 532/546/555 Pro-Q Diamond BODIPY 576/589 R-phycoerythrin RFP HNPP 630 nm LED Alexa Fluor 633/635 Alexa Fluor 647/700 Cy5 BODIPY 650/665 DiD TOTO 3 DDAO Phosphate 3 www.gelifesciences.co.jp 93

3 Amersham Imager 680 LAS Amersham Imager PC 100 300 dpi NEW PDF 16-bit tif 94 www.gelifesciences.co.jp

OD 1 Amersham Imager 680 QC RGBOD IQ/OQ ImageQuant TL ImageQuant TL SecurITy OD Trans 3 1 OD: Optical Density ImageQuant TL SecurITy ImageQuant TL SecurITy 1D ImageQuant TL 103 Amersham Imager 680 RGB System 2 NEW 8,819,200 Amersham Imager 680 QC System 2 NEW 6,379,200 Amersham Imager 680 UV System 2 NEW 5,759,200 Amersham Imager 680 System 2 NEW 4,739,200 2 Software 102 103 135 92 138 www.gelifesciences.co.jp 95

3 Amersham Imager 680 Amersham Imager 600! // / / 10 50 Amersham Imager 600680 Amersham Imager Amersham Imager 680 Amersham Imager 600 NIH / 3T3 ERK 9.8 ng ERK NIH/3T3 cell lysate 5 µg 9.8 ng 2 2.5 pg Transferrin 625 pg 2.5 pg Log integrated intensity 8.0 7.5 7.0 6.5 6.0 5.5 5.0 4.5 4.0 0.5 1.5 2.5 3.5 4.5 Log cell lysate amount (ng) Log integrated intensity 8.0 7.0 6.0 5.0 4.0 3.0 0.0 1.0 2.0 3.0 Log protein amount (pg) NIH / 3T3 5 g9.8 ng 2 Amersham Hybond P PBS-T Amersham ECL Prime2 ERK1 / 2 1:10,000 HRP IgG 1:100,000 Amersham ECL Select 2.7 625 pg2.5 pg2 Amersham Hybond P PBS-T 3 BSA 1:1,000 HRP IgG 1:75,000 Amersham ECL Select LOD 2.5 pg HRP: 96 www.gelifesciences.co.jp

Amersham ECL Plex Cy2 Cy3 Cy5 Cy5 Phosphorylase b 3.3 Phosphorylase b 200 ng 98 pg Log integrated intensity 8.0 7.0 6.0 5.0 4.0 3.0 1.0 2.0 3.0 4.0 5.0 6.0 Log protein amount (pg) LMW 200 ng2 Phosphorylase b Cy5 Cy5 98 pg Phosphorylase b 3.3 9E.coli DHFR Cy3 Cy5 DHFR Prelabeling Cy5 Total protein DNA KiloBase DNA3 SYBR Green KiloBase DNA Marker 250 ng 0.3 ng Log integrated intensity KiloBase DNA 3 SYBR Green I Cy2 0.3 ng DNA 2.9 Tips 8.0 7.5 7.0 6.5 6.0 5.5 5.0 4.5 4.0 1.5 2.5 3.5 4.5 5.5 Log amount of total DNA (pg) GAPDH CV 10% CHO ERK1 / 2 Cy5 ERK1 / 2 CV CHO (µg) 3 2.5 5 10 15 20 kda 225 WB: DHFR Cy3 97 66 50 Cy3/Cy5 overlay 35 25 Cy3 ERK 1/2 K 20 14 10 Cy5 β tubulin β actin GAPDH 0.80 0.70 6.83 Overlay Normalized ratio Cy3/Cy5 0.70 0.0733 0.0696 0.0694 0.0733 0.0701 0.0556 0.60 0.063 0.0627 0.062 0.0605 0.50 0.40 0.30 0.20 Normalized ratio Cy3/Cy5 6.11 0.60 0.50 0.40 0.30 0.20 5.45 6.16 4.23 4.48 4.45 4.64 4.35 4.53 0.10 0.10 0 4 5 6 7 8 9 10 11 12 13 Lane number 0 4 5 6 7 8 9 10 11 12 13 Lane number 1 2 3 4 5 6 7 8 9 PBS-T 3 BSA DHFR C 1:1,000 Cy3 IgG 1:2,500 Cy3 Cy5 Loading control Variation of normalized ratio between 2.5 to 20 g sample load (CV %) Cy5 Total protein 7 Tubulin 18 Actin 14 GAPDH 53 Cy5Amersham QuickStain Asa Hagner-McWhirter, et al, Cy5 total protein normalization in Western blot analysis, Analytical Biochemistry 486 (2015) 54 61 www.gelifesciences.co.jp 97

3 ImageQuant LAS 500 SYBR Green ImageQuant TL SYBR Green SYPRO Ruby SYBR Green CBB 1 CBB 2 3 ImageQuant LAS 500 Ready X X ImageQuant LAS 500 ImageQuant LAS 500 System 2,573,000 Software 103 135 92 138 98 www.gelifesciences.co.jp

RI 3 Amersham Typhoon scanner RGB NIR RI 40 46 cm 1 5 8 ImageQuant TL IQ/OQ Eraser 1 Phosphor Imaging RI 35 43 cm Cabinet PMT 2 PMT 2 PMT : Photomultiplier tube 3 Use PMT PMT 2 PMT 1 4 Cy3 Typhoon FLA 9500 409.6 204.8 102.4 51.2 25.6 12.8 6.4 3.2 1.6 0.8 0.4 0.2 ng Amersham Typhoon 2.8 1.4 0.7 0.36 0.18 0.09 0.045 0.02 ng 8 12 /532 nm / BPG1 570DF20 3.3 R2 = 0.998 log (integrated intensity) 6 4 2 1 2 3 4 5 6 log (pg phosphorylase B) LMW Phosphorylase b 8-18 SDS-PAGE /532 nm / Cy3 570BP20 4.2 R2 = 0.9998 3 2 1 CHO ERK GAPDH DaTscan 3 123I-ioflupane PBS ph 7.4 1 nm DaTscan 60 3 3 PBS SR 60 CHO SDS-PAGE 8-18 Amersham Hybond P 0.45 ERK GAPDH ECL Plex Cy3 IgG Alexa Plus 800 IgG Amersham QuickStain Excitation Emission filter 532 nm Cy3 570BP20 (green) 685 nm IRshort 720BP20 (red) 785 nm IRlong 825BP30 (blue) 3 DaTscan GE Healthcare SPECT 4 Sergio Estrada, Preclinical PET-MRI Platform, Dept. of Medicinal Chemistry, Uppsala University. 4 60 SR 635 nm / IP 390BP www.gelifesciences.co.jp 99

3 Phosphor Imaging Digitization (Densitometry) 1 Amersham Typhoon scanner 5 System Amersham Typhoon scanner RGB System Amersham Typhoon scanner NIR Plus System Amersham Typhoon scanner NIR System Amersham Typhoon scanner IP System 2 Fluorescence RGB 3 Green Fluorescence IR1, IR2 3 Chemiluminescence (Dark scan) 4 IP Stage Fluor Stage Multi Stage 488 nm LD 532 nm SHG 635 nm LD 685 nm LD 785 nm LD IP 390BP Cy2 525BP20 Cy3 570BP20 Cy5 670BP30 IRshort 720BP20 IRlong 825BP30 IP Stage Fluor Stage Multi Stage 488 nm LD 532 nm SHG 635 nm LD IP 390BP Cy2 525BP20 Cy3 570BP20 Cy5 670BP30 Fluor Stage Fluor Stage IP Stage 532 nm SHG 685 nm LD 785 nm LD Cy3 570BP20 IRshort 720BP20 IRlong 825BP30 685 nm LD 785 nm LD IRshort 720BP20 IRlong 825BP30 635 nm LD IP 390BP 8 8 10 25 50 100 200 m 1,000 m 10 25 50 100 200 m PMTPMT PMT PMT 65,536 16-bit 5 40 46 cm 5 40 46 cm 35 43 cm 6 18 7 18 8 18 7 16-bit tif gel 16-bit img 900 800 400 mm W D H 94 kg 93 kg 92 kg 100 240 VAC 10 0.3 kva 50/60 Hz 18 28 20 70% ImageQuant TL 18,375,800 15,075,800 12,175,800 11,375,800 10,375,800 1 2 3 4 5 6 7 8 S Amersham Typhoon Digitization plate PMT LD Laser DiodeSHG Secondary Harmonic Generation 35 43 cm Phosphor Imaging 40 46 cm Phosphor Imaging50 m 20 25 cm Fluorescence Normal speed 50 m 20 25 cm Software 102 103 135 138 100 www.gelifesciences.co.jp

2D- HCP 3HCP ELISA 1D-PAGE HCP HCP 2D-PAGE 2D-DIBE 2D differential in blot electrophoresis HCP HCP HCP 2D- 2D difference gel electrophoresis ELISA 2D- HCP 2D-DIBE Cy3 labelled HCP protein Method ph 3 ph 10 Protein labeled with Cy3 Separation in 1 st Dimension by PI Separation in 2 nd Dimension by MW 2D- CBB HCP ph 3 ph 11 Harvest Flowthrough Protein transferred to membrane Eluate IgG hc IgG lc 1-D SDS-PAGE Coverage analysis WB: anti-hcp + Cy5 conjugated 2 nd Ab N2A - N2A 96 DRAQ5 n=4 1.20 1.00 0.80 0.60 0.40 0.20 0.00 Control RA 濃度 Low Middle High IRlong - IRshort Control RA Low RA Middle RA High AnBbody Only Normalized with cell number Cell number : : Neuro-2a N2A RA Low Middle High - CHROMIS 770A IgG DRAQ5 96 Amersham Typhoon scanner NIR / DRAQ5 685 nm IRshort 720BP20/ CHROMIS 770A 785 nm IRlong 825BP30 100 m + ImageQuant TL version 8.2 1 RA - RA - www.gelifesciences.co.jp 101

3 Ettan Ettan 2D- Ettan CyDye Typhoon 2D- Ettan 5800 1 1 2018 4 2D- HCP Melanie Melanie Classic Cy3 Cy5 Cy2 Typhoon 3D Cy2 Cy3 Cy5 & 1 Fluorescence 2D difference gel electrophoresis 2D- Melanie 2D- HCP Melanie Melanie Coverage Melanie Classic Melanie 2way-ANOVA Melanie Melanie Coverage HCP Coverage Assay Melanie Coverage Melanie Classic Melanie 136 Melanie 9 Classic Node-locked 1 2 NEW 1 29270534 971,500 Melanie 9 Node-locked 1 2 NEW 1 29270536 1,943,000 Melanie 9 Coverage Node-locked 1 2 NEW 1 29270543 3,644,000 2 PC Node-locked Floating Web Scanner Ettan CyDye Fluors, Minimal Labeling Dyes Cy2 / Cy3 / Cy5 3 NHS-ester Minimal Labeling Ettan Web CyDye Fluor, minimal labeling kit 2 nmol 3 1 28934530 299,000 CyDye Fluor, Cy2 minimal dye 5 nmol 25801082 167,500 CyDye Fluor, Cy3 minimal dye 5 nmol 25801083 167,500 CyDye Fluor, Cy5 minimal dye 5 nmol 25801085 167,500 CyDye Fluor, minimal labeling kit 5 nmol 3 1 25801065 445,200 102 www.gelifesciences.co.jp

3 ImageQuant TL IQTL 1D 1 1D 4 1D ImageQuant TL SecurITy 135 ImageQuant TL SecurITy ImageQuant TL SecurITy 1D ImageQuant TL 8.2 Node-locked 1 NEW 1 29291744 70,000 ImageQuant TL 8.2 Node-locked 5 NEW 1 29291746 300,000 ImageQuant TL SecurITy 8.2 Node-locked 1 1 NEW 1 29291745 150,000 ImageQuant TL PC 2 NEW 1 1ImageQuant TL ImageQuant TL SecurITy Software 2 LapTop PC DeskTop PC Node-locked Floating Web Amersham QuickStain Cy5 15 40 1 µg/ml 20 mg/ml ng Buffer Cy5 Cy5 QuickStain Cy3 Cy3 Sample QuickStain Amersham QuickStain kit 150 samples 10-15 gels RPN4000 17,300 Amersham ECL Plex 106 www.gelifesciences.co.jp 103

ウェスタンブロッティング 画像解析 3 ウェスタンブロッティング検出試薬選択ガイド ECL Select 推奨アプリケーション 感 最高感度の化学発光検出 一次抗体希釈率の目安 1:5,000 1:30,000 二次抗体希釈率の目安 推奨メンブレン 検出方法 カメラタイプ 電気泳動 ECL start 一般的な化学発光検出 ECL に対して 40 100 倍の感度 一般的な化学発光検出 高発現タンパク質検出 タグ付きタンパク質検出 ピコグラムオーダーの タンパク質を検出可能 1 1,000 1 30,000 2 1:100,000 1:300,000 2 1 500 1 5,000 2 1 50,000 1 250,000 2 ECL と同等 1 500 1 3,000 2 2 1 2,500 1 15,000 2 1 5,000 1 50,000 2 2% ECL Prime Blocking Agent 2% ECL Prime Blocking Agent 5% ECL Blocking Agent RPN418 RPN418 RPN2125 5% ECL Blocking Agent RPN2125 5% ECL Blocking Agent RPN2125 一般的な試薬 5% スキムミルク 一般的な試薬 5% スキムミルク 一般的な試薬 5% スキムミルク 3% BSA など 3% BSA など 3% BSA など 5% ECL Blocking Agent RPN2125 一般的な試薬 5% スキムミルク 3% BSA など 対応 対応 対応 対応 Amersham Hybond PVDF, Amersham Protran Premium NC Amersham Hybond PVDF, Amersham Protran Premium NC Amersham Hybond PVDF, Amersham Protran Premium NC Amersham Hybond PVDF, Amersham Protran Premium NC X 線フィルム Hyperfilm ECL X 線フィルム Hyperfilm ECL X 線フィルム Hyperfilm ECL X 線フィルム Hyperfilm ECL イメージャー Amersham Imager シリーズ ImageQuant LAS 500 イメージャー Amersham Imager シリーズ ImageQuant LAS 500 イメージャー Amersham Imager シリーズ ImageQuant LAS 500 イメージャー Amersham Imager シリーズ ImageQuant LAS 500 3 スキャナー タイプ ウェスタン ブロッティング 高感度な化学発光検出 ECL Prime に対して 2 8 倍高感度 度 1 ブロッキング剤 ECL ターゲットタンパク質や一次抗体の 発光安定性が高いため多検体処理 過剰発現タンパク質の検出 量が少ない場合に も余裕をもって実験可能 タグ付きタンパク質の検出 リプロービング Amersham Hybond を使用 カメラタイプ 機種一覧 ECL Prime 1.2 µg 5 ng 5 µg 20 ng 10 µg 160 ng 10 µg 検出結果の例 WB ERK ½; LOD: 5 ng; DR: 2.4 1 3 WB ERK ½; LOD: 320 ng; DR: 2.1 WB ERK ½; LOD: 20 ng; DR: 2.4 WB ERK ½; LOD: 320 ng; DR: 2.1 感度 発光時間は実験条件によりかわることがあります 2 PVDF メンブレン使用時の目安 最良の結果を得るためにはサンプルごとに条件検討を行って至適抗体濃度を決定する必要があります LOD = Limit of Detection DR = Detection Range ECL シリーズ感度比較 目安 ECL Select 関連製品 ECL Prime ECL / ECLthe start We compared sensitivity and signal intensity of Amersham ECL Select to that of Amersham ECL Advance 高感度 in a Western 中程度 blotting experiment to detect ERK ½. Both reagents showed very high sensitivity and produced equal detection limits for ERK ½ (5 ng total protein) (Fig 5A and B). However, Amersham ECL Select produced 3-fold higher signal intensity (Fig 5C) with a sustained low background, resulting in brighter and clearer protein bands. We used optimized antibody dilutions for each detection reagent and each blot was exposed for 1 min using ImageQuant LAS 4000 imaging system. ECL シリーズ最高感度 Signal intensity and duration We compared the signal intensity and duration of 検出限界 超高感度 Amersham ECL Select to that of Amersham ECL Advance for Western blotting detection of ERK ½ in HeLa cell lysate. We detected the emitted signals immediately after addition of the respective detection reagent, and followed the signal いっしょに intensity at time points up to two hours after 購入すると便利 reagent addition. Blots for Amersham ECL Select and Amersham ECL Advance were imaged side by side, using same 常温保存 exposure times. Amersham ECL Select gives a significantly できるように higherなりました signal output, with sufficient signals remaining 2 h after addition of the reagent, even for the lowest amount バックグラウンドが低く 幅広い実験条件に対応 Sample: HeLa cell lysate in a 2-fold dilution series starting of protein tested. This gives you a convenient time window at 5 µg total protein シグナルの安定性が高く 長時間にわたる多サンプル実験にも好適 between the end of an experiment and the beginning of Membrane: Amersham Hybond-P analysis thus allowing for multiple exposures. ECL シリーズ最高感度の検出試薬 Amersham ECL Select Blocking: Primary Ab: Amersham ECL Prime blocking agent (2% in PBS-T) Rabbit anti ERK ½ diluted 1:10 000 ご注文情報 (Amersham ECL Selelect), and 1:30 000 製品 包装 (Amersham ECL Advance) 2 ECL Select Ab: Western Blotting Detection Reagent for 1,000 membrane Secondary HRP conjugated anti-rabbit IgG cm diluted 1:100 000 40 g ECL Prime Blocking Reagent (Amersham ECL Select), and 1:300 000 (Amersham ECL Advance) ECL start ECL Prime ECL Select ECL Plex 用です Imaging: LAS 4000 サンプル については 144ImageQuant ページで請求方法等ご案内しています Exposure time: 1 min ECL Select と ECL Prime の検出限界の比較 A) ECL SelectECL Select Amersham Amersham ECL Select 55 µg 5 µg B) ng 55 ng 5 ng 5Amersham µg ECL Advance ECL PrimeECL Advance Amersham 5 µg 55 µg 20 ng 5 ng 55 ng Sample: コード番号 HeLa cell lysate in a 2-fold dilution series starting at 5 µg total protein RPN2235 サンプル 50,000 Membrane: Amersham Hybond-P RPN418 13,000 Blocking: Amersham ECL Prime blocking agent (2% in PBS-T) Primary Ab: Rabbit anti ERK ½ diluted 1:10 000 (Amersham ECL Selelect), and 1:30 000 (Amersham ECL Advance) Secondary Ab: HRP conjugated anti-rabbit IgG diluted 1:100 000 (Amersham ECL Select), and サンプル HeLa 細胞のライセートを 5 μ g から段階的に 2 倍希釈したもの 1:300 000 (Amersham ECL Advance) メンブレン Hybond-P Imaging: ImageQuant LAS 4000 ブロッキング agent 2% in PBS-T Exposure time: ECL Prime3blocking min ウサギ抗 ERK 抗体 一次抗体 ECL Select 10,000 倍希釈 ECL Prime 3,000 倍希釈 Amersham ECL Advance Time (min) Amersham ECL Select HRP 標識抗ウサギ IgG 二次抗体 ECL Select 100,000 倍希釈 ECL Prime 30,000 倍希釈 0 ImageQuant LAS 4000 検出 露出時間 1分 30 WB ERK ½; LOD: 20 ng; DR: 2.4 C) Amersham ECL Select www.gelifesciences.co.jpamersham ECL Advance 4000 ity ( 10-4) 104 5000 60 120 価格 円 160 ng

Amersham ECL Prime 1 1 1 60 ECL Prime Western Blotting Detection Reagent for 1,000 cm 2 membrane RPN2232 35,400 ECL Prime Western Blotting Detection Reagent for 3,000 cm 2 membrane RPN2236 76,200 ECL Prime Blocking Reagent 2 40 g RPN418 13,000 ECL Prime Starter Kit Core 3 for 1,000 cm 2 membrane 72AS0120 48,300 ECL Prime Starter Kit Core DualVue 3 for 1,000 cm 2 membrane 72AS0121 65,300 2 ECL Start ECL Prime ECL Select ECL Plex 3 144 ECL Prime RPN2232 HRP ECL Prime Starter Kit Core 4 5 ECL Prime Starter Kit Core DualVue 4 5 6 4 HRP 100µl 5 ECL Prime Blocking Reagent RPN418 6 ECL DualVue Western Blotting Markers RPN810 ECL Prime( 露出 75 秒 ) ECL Plus( 露出 3 分 ) 2.5 ng 2.4 pg 2.5 ng 9.8 pg ECL Plus 2.4 pg 2.5 ng 12000 2000 ダイナミックレンジ ダイナミックレンジ 2 (min) ECL Prime ImageQuant LAS 4000mini 2.5 ng 4.9 pg 0 30 ECL Prime 1 60 3 ECL start Amersham ECL start ECL HRP ECL start Western Blotting Detection Reagent for 2,000 cm 2 membrane RPN3243 13,900 ECL start Western Blotting Detection Reagent for 4,000 cm 2 membrane RPN3244 23,900 ECL Prime Blocking Reagent 2 40 g RPN418 13,000 2 ECL start ECL Prime ECL Select ECL Plex 144 ECL Hela ERK 1/2 ECL 1,000 HeLa whole cell lysate ECL start Anti-ERK-1/2, rabbit antibody (Sigma- 100 Aldrich ) 5,000 Anti-Rabbit IgG, HRP-Linked Whole Ab ECL ECL Donkey (GE Healthcare) 10,000 ECL start 10 µg Amersham WB system Amersham Imager 600 1 Lower tray position 5min 0.1 1 10 HeLa whole cell lysate (µg Protein) ImageQuant TL v8.1 Analysis Toolbox 10 5 2.5 1.25 0.625 0.313 0.156 Integrated signal Intensity ( 10 4 ) www.gelifesciences.co.jp 105

3 ECL Amersham ECL HRP Amersham ECL Plex ECL Plex goat- -mouse IgG-Cy5 150 µg PA45009 25,600 ECL Plex goat- -rabbit IgG-Cy5 150 µg PA45011 25,600 ECL Plex goat- -mouse IgG-Cy3 150 µg PA43009 25,800 ECL Plex goat- -rabbit IgG-Cy3 150 µg 28901106 34,400 ECL Plex Fluorescent Rainbow Markers 500 µl RPN851E 36,700 ECL Plex Fluorescent Rainbow Markers 120 µl RPN850E 9,700 ECL Western Blotting Starter Kit 1 RPN2108 28,300 ECL Western Blotting Detection Reagents ECL Western Blotting Detection Reagents ECL Western Blotting Detection Reagents for 1,000 cm 2 membrane RPN2109 11,700 for 2,000 cm 2 membrane RPN2209 18,200 for 4,000 cm 2 membrane RPN2106 31,400 ECL Blocking Agent 40 g RPN2125 8,400 ECL 1 Amersham Hybond 3 12 ECL 20 1 100 1 5,000 2 1 1,250 1 4,000 2 5% BSA 2% ECL Prime Blocking Reagent RPN418 Amersham Hybond LFP PVDF, Amersham Protran Premium NC Typhoon FLA Amersham Imager 600 RGB 10 µg 5 ng WB ERK ½ (Cy5 on FLA 9500); LOD 10 ng; DR: 3 Silver Staining Kit, Protein 1 17115001 31,200 Bind-Silane 25 ml 17133001 14,500 Acrylamide PAGE 1 kg 17130202 21,200 N,N'-Methylene-bisacrylamide 100 g 17130402 24,700 Tris 500 g 17132101 22,600 Sodium Dodecylsulphate 100 g 17131301 7,600 Ammonium Persulphate 25 g 17131101 6,200 Glycerol, 87 % 1 L 17132501 10,000 Dithiothreitol 1 g 17131801 9,600 Dithiothreitol 5 g 17131802 30,500 1 2 Amersham Hybond LFP PVDF 3 LOD = Limit of Detection DR = Detection Range Glycine 500 g 17132301 7,900 Protease Inhibitor Mix 1 ml 80650123 34,600 Thiourea 100 g RPN6301 10,100 Urea 500 g 17131901 7,700 CHAPS 1 g 17131401 18,100 TEMED 25 ml 17131201 6,500 Bind-Silane 25 ml 17133001 14,500 Repel-Silane ES 500 ml 17133201 15,400 Iodoacetamide 25 g RPN6302 17,800 106 www.gelifesciences.co.jp

LMW Marker Kit 14.4 97.0 kda 10 vials 576 µg/vial CBB 40 /vial HMW SDS Marker Kit 53.0 220 kda CBB 10 vials 175 µg/vial CBB 20 /vial Unstained HMW Native Marker Native-PAGE CBB 66.0 669 kda Kit 10 vials 250 µg/vial 20 /vial Amersham Rainbow Molecular Weight Markers Full, High, Low 3 Amersham ECL DualVue Western Blotting Markers Amersham ECL Plex Fluorescent Rainbow Markers Pre-stained Pre-stained S-tag Pre-stained 12 225 kda Full High 250 µl 50 3.5 38 kda Low 15, 16, 100 kda 15 150 kda HRP 12 225 kda X 25 120 µl 40 80 S-Protein-HRP Cy3/Cy5 3 LMW HMW SDS HMW -Native Rainbow Markers Full-range RPN800E Rainbow Markers High-range RPN756E Rainbow Markers Low-range RPN755E kda kda kda 225 225 150 102 76 76 38 31 24 52 52 17 38 31 24 38 31 24 12 8.5 3.5 M r 10 3 100 M r 10 3 150 100 75 17 12 17 12 50 Rainbow Molecular Weight Marker 35 25 16 15 15 1 ECL DualVue 2 3 4 5 6 Lane1 ECL DualVue 3 Lane2&6 ECL Dualvue 7 S-tagged ECL Plex Fluorescent Rainbow Markers 120 µl RPN850E 9,700 ECL DualVue Western Blotting Markers 25 gel loadings RPN810 21,200 Full-Range Rainbow Molecular Weight Markers 250 µl RPN800E 21,500 High-Range Rainbow Molecular Weight Markers 250 µl RPN756E 16,600 Low-Range Rainbow Molecular Weight Markers 250 µl RPN755E 16,600 LMW Marker Kit 10 vials, 575 µg/vial 17044601 45,200 HMW SDS Marker Kit 10 vials, 175 µg/vial 17061501 47,800 HMW Native Marker Kit 10 vials, 250 µg/vial 17044501 45,200 www.gelifesciences.co.jp 107

3 Amersham Western Blotting PVDF Amersham Hybond PVDF 0.2 µm Amersham Hybond 0.2 µm 0.45 µm Amersham Hybond P PVDF 80 90 mm 25 10600101 35,000 10600100 35,000 Amersham Hybond P PVDF 100 100 mm 10 10600087 16,500 Amersham Hybond P PVDF 100 100 mm 25 10600086 39,500 Amersham Hybond P PVDF 140 160 mm 25 10600069 47,500 Amersham Hybond P PVDF 200 200 mm 10 10600058 22,500 Amersham Hybond P PVDF 200 200 mm 25 10600057 53,000 Amersham Hybond P PVDF 300 600 mm 5 10600038 52,500 Amersham Hybond P PVDF 150 mm 4 m 1 10600029 35,500 Amersham Hybond P PVDF 260 mm 4 m 1 10600021 41,000 Amersham Hybond P PVDF 300 mm 4 m 1 10600023 41,500 Amersham Hybond P Sandwich PVDF+3MM 80 90 mm 10 10600122 14,800 10600121 14,800 Amersham Hybond LFP 0.2 µm Amersham Hybond LFP PVDF 80 90 mm 25 10600102 37,000 Amersham Hybond LFP PVDF 200 200 mm 10 10600060 24,500 Amersham Hybond LFP PVDF 254 mm 4 m 1 10600022 43,000 Amersham Hybond LFP Sandwich PVDF+3MM 80 90 mm 10 10600123 15,600 Amersham Hybond SEQ 0.2 µm Amersham Hybond SEQ PVDF 260 mm 4 m 1 10600030 41,000 144 Amersham 3MM Chr 8 9 cm 14 16 cm 2 Cathode(-) Upper filter paper Gel Membrane Lower filter paper Anode(+) Amersham 108 www.gelifesciences.co.jp

Amersham Western Blotting Amersham Protran ECL Amersham ECL 3 Amersham Protran 0.1 µm 0.2 µm 0.45 µm Amersham Protran NC 80 90 mm 25 10600094 35,000 10600093 35,000 Amersham Protran NC 100 100 mm 10 10600073 16,500 Amersham Protran NC 140 160 mm 25 10600062 47,500 Amersham Protran NC 200 200 mm 10 10600046 22,500 10600044 22,500 10600042 22,500 Amersham Protran NC 200 200 mm 25 10600045 53,000 10600043 53,000 10600041 53,000 Amersham Protran NC 300 600 mm 5 10600032 52,500 10600033 52,500 Amersham Protran NC 150 mm 4 m 1 10600010 35,500 10600011 35,500 10600012 35,500 Amersham Protran NC 200 mm 4 m 1 10600005 38,500 10600006 38,500 10600007 38,500 Amersham Protran NC 300 mm 4 m 1 10600000 41,500 10600001 41,500 10600002 41,500 Amersham Protran Sandwich NC+3MM 80 90 mm 10 10600116 14,800 10600115 14,800 10600114 14,800 Amersham Protran Premium 0.2 µm 0.45 µm Amersham Protran Premium NC 80 90 mm 25 10600096 35,000 Amersham Protran Premium NC 100 100 mm 10 10600079 16,500 Amersham Protran Premium NC 100 100 mm 25 10600080 39,500 Amersham Protran Premium NC 140 160 mm 25 10600065 47,500 Amersham Protran Premium NC 200 200 mm 10 10600048 22,500 Amersham Protran Premium NC 200 200 mm 25 10600047 53,000 Amersham Protran Premium NC 300 600 mm 5 10600034 52,500 Amersham Protran Premium NC 150 mm 4 m 1 10600014 35,500 10600013 35,500 Amersham Protran Premium NC 200 mm 4 m 1 10600009 38,500 10600008 38,500 Amersham Protran Premium NC 300 mm 4 m 1 10600004 41,500 10600003 41,500 Amersham Protran Premium Sandwich NC+3MM 80 90 mm 10 10600118 14,800 10600117 14,800 Amersham Protran Supported 0.2 µm 0.45 µm Amersham Protran Supported NC 200 200 mm 25 10600053 55,000 10600051 55,000 Amersham Protran Supported NC 300 600 mm 5 10600037 54,500 Amersham Protran Supported NC 150 mm 4 m 1 10600019 37,500 10600020 37,500 Amersham Protran Supported NC 200 mm 4 m 1 10600017 40,500 10600018 40,500 Amersham Protran Supported NC 300 mm 4 m 1 10600015 43,500 10600016 43,500 Amersham Protran Supported Sandwich NC+3MM 80 90 mm 10 10600120 15,600 10600119 15,600 www.gelifesciences.co.jp 109

3 Ettan IPGphor 3 IEF System Strip Holder 10,000 V 7 11 13 18 24 cm Immobiline DryStrip 12 15 30 21.5 ma 10,000 V 12 W 278 471 160 mm W D H 8.3 kg Ettan IPGphor 3 Isoelectric Focusing Unit 2 1 11003364 1,364,000 1 Ettan IPGphor 3 Isoelectric Focusing Unit Ettan IPGphor 3 Control Software Strip Holder 2 7 11 13 18 24 cm Strip Holder 2 Control Software PC 2 Ettan IPGphor 3 Control Software Control Software PC SE 260 Mighty Small II Mini-Vertical Units 10 10.5 cm 10 10 cm 7 cm Immobiline DryStrip 135 136 112 138 165 160 180 mm W D H80 95 mm 100 105 mm 75 ml 250 ml500 V 500 ma 12 W SE 260 Mighty Small II Mini-Vertical Unit for 2 slab gels, Complete 1 SE260-10A-.75 160,500 Mighty Small Dual Gel Caster 10 10.5 cm 10 10 10.5 cm 2 10 0.75 mm 4.8 mm 2 0.75 mm 10.5 cm 2 111 SE 600 Ruby Standard Vertical Units 18 16 cm 4 Immobiline DryStrip 11 cm 13 cm 320 140 290 mm W D H 140 160 mm 180 160 mm 1,000 V500 ma 50 W 1cm 16 16cm 111 135 SE 600 Ruby Standard Dual Cooled Vertical Unit, Complete 1 80647957 353,300 Duel Gel Caster 1 or 2 gels 18 16 cm 3 16 cm 4 6 15 1.50 mm 5.7 mm 2 1.50 mm 2 cm 16 cm 2 110 www.gelifesciences.co.jp

minive Vertical Electrophoresis System Gel module 1 10 10.5 cm 10 10 cm Blot module 192 172 188 mm W D H80 95 mm 100 105 mm80 70 mm 100 80 mm 100 ml 100 105 mm 75mm 100 80 mm 1.2 L 1 1.7 L 2 600 V 25 W Blot module 90 105 mm300 ml2 5 75 400 ma 15 W minive Vertical Electrophoresis Unit, Complete 1 80641877 185,300 Gel module 2 10 10.5 cm 3 10 10.5 cm 3 10 1.0 mm 4.8 mm 2 1.0 mm 10.5 cm 2 111 3 Amersham ECL Semidry Blotters TE 70 PWR / TE 77 PWR 0.8 ma/cm 2 TE 70 140 160 mm 2 TE 77 210 260 mm 4 2 : 0 30 V : 1 500 ma4 40 80380 460 90 mm W D H 3.7 kgte 70/TE 77 CE 73/23/EEC LV directive EN-61010-1 IEC 1010-1, UL3101-1, CSA22.2 1010-1 TE 70 PWR/TE 77 PWR EN 61326 TE 70 PWR Semi-dry Transfer Unit, 14 16 cm 1 1 11001341 248,900 TE 77 PWR Semi-dry Transfer Unit, 21 26 cm 2 1 11001342 295,100 1 25 5016.5 18.5 cm 7 8 cm 14 16 cm 2 25 5023 28 cm 14 16 cm EPS 301 Power Supply EPS 3501XL Power Supply V ma W EPS 301 300 400 80 1 1 1 min 24 h EPS 3501XL 3,500 400 200 9 9 1 min 500 h Vh 1 Vh 500 kvh Ah 1 mah 50 Ah 4 mm 2 mm EPS 301 Power Supply 1 18113001 118,500 EPS 3501XL Power Supply 1 18113005 366,900 www.gelifesciences.co.jp 111

3 Immobiline DryStrip 1 ph6-11 ph No. 1 1 Immobiline DryStrip ph 4-7, 24 cm 12 17600246 39,000 Immobiline DryStrip ph 3-11 NL, 13 cm 2 12 17600375 28,500 Immobiline DryStrip ph 6-9, 24 cm 12 17600247 39,000 Immobiline DryStrip ph 4-7, 11 cm 12 18101660 26,700 Immobiline DryStrip ph 3-10, 24 cm 12 17600244 39,000 Immobiline DryStrip ph 6-11, 11 cm 12 17600195 26,700 Immobiline DryStrip ph 3-10 NL, 24 cm 2 12 17600245 39,000 Immobiline DryStrip ph 3-10, 11 cm 12 18101661 26,700 Immobiline DryStrip ph 3-11 NL, 24 cm 2 12 17600377 39,000 Immobiline DryStrip ph 3-11 NL, 11 cm 2 12 17600374 26,700 Immobiline DryStrip ph 4-7, 18 cm 12 17123301 30,300 Immobiline DryStrip ph 4-7, 7 cm 12 17600110 21,300 Immobiline DryStrip ph 6-11, 18 cm 12 17600197 30,300 Immobiline DryStrip ph 6-11, 7 cm 12 17600194 21,300 Immobiline DryStrip ph 3-10 NL, 18 cm 2 12 17123501 30,300 Immobiline DryStrip ph 3-10 NL, 7 cm 2 12 17600112 21,300 Immobiline DryStrip ph 3-10, 18 cm 12 17123401 30,300 Immobiline DryStrip ph 3-10, 7 cm 12 17600111 21,300 Immobiline DryStrip ph 3-11 NL, 18 cm 2 12 17600376 30,300 Immobiline DryStrip ph 3-11 NL, 7 cm 2 12 17600373 21,300 Immobiline DryStrip ph 4-7, 13 cm 12 17600113 28,500 Immobiline DryStrip ph 6-11, 13 cm 12 17600196 28,500 Immobiline DryStrip Cover Fluid 3 1 L 17133501 10,700 Immobiline DryStrip ph 3-10 NL, 13 cm 2 12 17600115 28,500 Silicone Oil 1 kg 71150050 20,200 Immobiline DryStrip ph 3-10, 13 cm 12 17600114 28,500 2 NL non-linear ph gradient ph Web Q&A 3 Immobiline DryStrip IPGphor IPG Buffer phimmobiline DryStrip IPG Buffer Immobiline DryStrip phimmobiline DryStrip IPG Buffer ph 4-7 1 ml 17600086 7,300 IPG Buffer ph 6-11 1 ml 17600178 7,300 IPG Buffer ph 3-10 1 ml 17600087 7,300 IPG Buffer ph 3-10 NL 1 ml 17600088 7,300 IPG Buffer ph 3-11 NL 1 ml 17600440 7,300 Immobiline DryStrip ph 6-9, 6-11 Immobiline DryStrip DeStreak Rehydration Solution DeStreak Rehydration Solution IPG Buffer DeStreak Rehydration Solution 1 5 3 ml 17600319 21,800 DeStreak Reagent 2 1 ml 17600318 10,700 1 IPG Buffer Immobiline DryStrip 2 1 ml 12 µl DeStreak Reagent 112 www.gelifesciences.co.jp

GE 12 12 1 1 1 2 3 4 5 6 7 MSDS: Material Safety Data Sheet Web 138 5 Web www.gelifesciences.co.jp 145

TEL 03-5331-9336 9:00 17:30 FAX 03-5331-9349 9:00 12:00 13:00 17:30 ÄKTA 1 2 3 IN Cell Analyzer 4 FAX 03-5331-9370 e-mail Tech-JP@ge.com 9:00 12:00 13:00 17:30 www.gelifesciences.co.jp GE Healthcare UK Ltd. Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, England GE 169-0073 3-25-1 TEL 03-5331-9336 FAX 03-5331-9370 e-mail Tech-JP@ge.com 18.05.220 EP ISO 9001:2008 2018 5 31 71-3492-08