Stratagene Products Mx3000P/Mx3005P PCR
Mx3000P/Mx3005P Mx3000P PCR 5FRET Mx3005P PCR 401403 Mx3000P PCR 401449 Mx3005P PCR SYBR Green I TaqMan PCR PCRThreshold line ROX TM Tm 12Multiple Experimental Analysis) OS 21 CFR part11 96PCR 0.2ml 96 Mx3000P 4 Mx3005P 5 TaqMan SNP 1
QPCR Solutions Mx3000P/Mx3005P Mx3000P / Mx3005P PMT 0.2 ml 96 PMT Mx3000P/Mx3005P 96 96 13.5 Mx3000P/Mx3005P PMT PMT Mx3000P/Mx3005P PMT PCR 8 5 Mx3000P 4 96 3.5 Level Take Next Multiplex QPCR to to the 96-well plate 2
Mx3000P/Mx3005P 2 ROX TM ROX TM 2 PCR 2 5 1FAM TM HEX TM ROX TM Cy TM 5 ALEXA Fluor 350 DNA5 PCR(ALEXA Fluor 350 - Alien, FAM TM - enos, HEX TM -HFE, ROX TM - CFTR, Cy TM 5 - Cyclophilin) Ct Mx3000P/Mx3005P PMT B7U 3 3 10 8 SYBR Green I PCR 9 PCR CGH Mx3000P 4 Mx3005P 5 PCR 3
QPCR Solutions PCR PCR PCR PCR PCR Mx3000P/Mx3005P Mx3000P/Mx3005P 2PCR Mx3005P FRET Duplex real-time PCR DNA (HEX TM DNA (FAM TM Mx3000P/Mx3005P PMT Mx3000P/Mx3005P Mx3005P DNA 2 FAM TM HEX TM 2 FAM TM HEX TM Ct S/N FAM TM HEX TM ROX TM Cy5 TM (%) FAM TM 2278 11 6 3 0.46% HEX TM 18 2714-2 -4 0.65% ROX TM -2 1 1034 14 1.32% Cy5 TM 1 11 5 2211 0.48% 4
MxPro PCR SYBR Green I PCR EvaGreen TM mirna SNPs ROX TM Assay name SYBR Green I PCR TaqMan ID Ready-to-Use TaqMan 2 3 PCR PCR PCR Run PCR Adaptive MxPro adaptive Threshold XML instrument data 5
QPCR Solutions Mx3000P/Mx3005P MxPro Amplification plot PCR SYBR & Multiplex Dissociation curve Raw data (first derivative) Standard curve Consolidated reports Standard curve ( Genotyping & SNP MxPro ΔΔ Ct Comparative quantification Relative quantity chart ( PCR Multiple Experimental Analysis Multiple Experiment Analysis Threshold line 12 Multiplex Experimental Analysis3 Threshold line IRC Inter-run calibrators 6
Mx3000P/Mx3005P PMT 1 3.5 /96/1 100V 10A 50-60Hz 10 60 µl / 200 µl PCR 96 Mx3000P Mx3005P 4 /1 5 /1 4 5 FRET Ramp Rate 7 10 350 750 nm 350 700 nm 0.25 2.5 / 25 95 36 cm54 cm45 cm20 kg PC Microsoft Windows Vista Pentium III Mx3000P Mx3005P ALEXA Fluor 350 350 nm 440 nm FAM TM /SYBR Green I 492 nm 516 nm TET 517 nm 538 nm HEX TM /JOE TM /VIC TM 535 nm 555 nm Cy TM 3 545 nm 568 nm TAMRA TM 556 nm 580 nm ROX TM /Texas Red 585 nm 610 nm Cy TM 5 635 nm 665 nm 7
QPCR Solutions Mx3000P/Mx3005P Mx3000P Mx3005P SYBR Green I PCR TaqMan PCR PCR 4 5 PCR LNA PCR FRETPCR * * PCR ** ** Tm SYBR Green/ TaqMan SNP SNP SNP Tm PicoGreen /RiboGreen EvaGreen TM mirna FRET * FRET PCR ** Mx3000P/Mx3005P SYBR Green I TaqMan Mx3000P/Mx3005P 8
Agilent 2100 PCR RNA PCR RNA FFPE RNA PCRRNA PCR Agilent 2100RNA Chip Agilent 2100 RNA RNA RIN RNA Integrity Number PCR RIN RNA HPRT13' 5' PCRRIN RNA Agilent 2100 SYBR Green I SYBR Green I 2 2 SYBR Green I Tm SYBR Green I Tm 9 marker 900, 1000 bp 473, 500 bp 300, 315 bp 100, 105 bp Agilent 2100 900, 1000 bp 473, 500 bp 300, 315 bp 100, 105 bp Agilent 2100 Agilent 2100 5 Agilent 2100 Agilent 2100
QPCR Solutions Agilent 2100 Lab-on-a-Chip Agilent 2100 Agilent 2100 Labon-a-Chip Chip 7,000 1 DNA RNA 3 1. 2. 3. Agilent 2100 Chip 21 CFR Part11 1 II DNA II RNA RIN=9.2 RNA RIN=5.8 0.1ng DNA II RNA RIN (RNA Integrity Number)RNA 50pg Total RNA II Protein 10
PCR SYBR GreenPCR SYBR GreenPCR 600828 Brilliant II SYBR Green QPCR Master Mix 400 600830 Brilliant II SYBR Green QPCR Low ROX TM Master Mix 400 600843 Brilliant II FAST SYBR Green QPCR Master Mix 400 SYBR Green 1-Step RT-PCR 600825 Brilliant II SYBR Green QRT-PCR Master Mix, 1-Step 400 600835 Brilliant II SYBR Green QRT-PCR Low ROX TM Master Mix, 1-Step 400 SYBR Green 2-Step RT-PCR 600834 Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix, 2-Step 400 PCR PCR 600804 Brilliant II QPCR Master Mix 400 600806 Brilliant II QPCR Low ROX TM Master Mix 400 600845 Brilliant II Fast Master Mix 400 1-Step RT-PCR 600809 Brilliant II QRT-PCR Master Mix, 1-Step 400 600837 Brilliant II QRT-PCR Low ROX TM Master Mix, 1-step 400 2-Step RT-PCR 600827 Brilliant II QRT-PCR AffinityScript Master Mix, 2-Step 400 PCR 600553 Brilliant Multiplex QPCR Master Mix 200 cdna 600559 AffinityScript QPCR cdna Synthesis Kit 50 Mx3000P/Mx3005P 401411 Mx3000P/Mx3005P Replacement Bulb Assembly 1 401334 96-Well QPCR Plates, Semi-Skirted 25 401333 96-Well QPCR Plates, Non-Skirted 25 401428 Mx3000P/Mx3005P Strip Tubes 120 401425 Mx3000P/Mx3005P Optical Strip Caps 120 410023 Single PCR Reaction Tubes (Without Caps) 1000 401421 MxP Perfect Fit Frame 1 RNA 750500 Stratagene QPCR Reference Total RNA, Human 25 µg 750600 Stratagene QPCR Reference Total RNA, Mouse 25 µg MVP RNA 11 Adult and Fetal Human Total RNA and Poly(A)+ RNA Mouse and Rat Total RNA and Poly(A)+ RNA
QPCR Solutions SideStepPCR 400904 Brilliant SYBR Green SideStep QPCR Master Mix 400 400909 SideStep II SYBR Green QRT-PCR Master Mix, 2-Step 400 400917 SideStep II QRT-PCR Master Mix, 1-Step 400 400918 SideStep II QRT-PCR Master Mix, 2-Step 400 400900 SideStep Lysis and Stabilization Buffer 10 400916 SideStep II Cell Lysate Analysis Kit 100 400908 SideStep II QPCR cdna Synthesis Kit 50 RNA Total RNA 400800 Absolutely RNA Miniprep Kit 50 400805 Absolutely RNA Microprep Kit 50 400753 Absolutely RNA Nanoprep Kit 50 400793 Absolutely RNA 96 Microprep Kit 2 RNA 400809 Absolutely RNA FFPE Kit 50 400811 Absolutely RNA FFPE Kit 50 RNA 400814 Absolutely RNA mirna Kit 50 PCR 200601 DNA Micro Extraction Kit 100 400766 StrataPrep DNA Gel Extraction Kit 50 400771 StrataPrep PCR Purification Kit 50 240218 StrataClone Ultra PCR Cloning Kit 20 240207 StrataClone Blunt PCR Cloning Kit 20 240205 StrataClone PCR Cloning Kit 20 mirna 600584 mirna QRT-PCR Master Mix kit 200 600583 mirna QPCR Master Mix 200 600580 High-Specificity mirna QRT-PCR Detection Kit 200 600545 High-Specificity mirna QPCR Core Kit 200 600036 mirna 1st Strand cdna Synthesis Kit 50 600035 Escherichia coli Poly (A) Polymerase 50 600700 600750 mirna Specific Forward Primer (200µl) 3.125 µm www.stratagene.com/mirna PCR 302107 MycoSensor QPCR Assay Kit 50 QPCR 300600 Alien QRT-PCR Inhibitor Alert 400 300602 Alien Reference RNA QRT-PCR Detection Kit (FAM TM detection) 100 300604 Alien Reference RNA QRT-PCR Detection Kit ( VIC TM detection) 100 12
Mx3000P/Mx3005P QPCR System Service Program Mx3000P/Mx3005PPCR 1 1 Mx3000P/Mx3005PPCR 1 401464-1 Mx3000P/Mx3005P 1 401464-2 Mx3000P/Mx3005P 2 401464-3 Mx3000P/Mx3005P 3 * * QC Mx3000P/Mx3005PPCRQC Mx3000P/Mx3005PPCR QC Mx3000P/Mx3005PPCR QC 600567 Mx3000P/Mx3005P QC 1 13
QPCR Solutions 21 CFR Part 11 MxPro ET MxPro21 CFR Part11 audit trail 21 CFR Part11 Mx3000P/Mx3005PPCR 401467 MxPro ET Software Activation Mx3000P/Mx3005PPCR 401468 MxPro ET Software Multi-User License Agreement 6 Beacon Designer PCRSYBR Green TaqMan FRETPCR / 401459 Beacon Designer / TaqMan Mfold* / Entrez dbsnp BLAST GenBank SNPSNP BLAST * http://frontend.bioinfo.rpi.edu/applications/mfold/cgi-bin/rna-form1.cgi 14
* This is a Licensed Real-Time Thermal Cycler(s) or Licensed Real-Time Temperature Cycling I nstrument(s) under ABI s United States Patent No. 6,814,934 and corresponding claims in non-u.s. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim. Absolutely mrna, AffinityScript, MxPro, and SideStep are trademarks of Stratagene in the United States. Absolutely RNA, Brilliant, FullVelocity, Mx3000P, and Mx3005P are registered trademarks of Stratagene in the United States. Agilent is a registered trademark of Agilent Technologies. Excel and PowerPoint are registered trademarks of the Microsoft Corporation. SYBR is a registered trademark of Molecular Probes. Scorpions is a trademark of DxS Ltd. TaqMan is a registered trademark of Roche Molecular Systems, Inc. TRI Reagent is a registered trademark of Molecular Research Center, Inc. FAM, TET, HEX, VIC, JOE, TAMRA, and ROX are trademarks of Applera Corporation or it s subsidiaries in the US and certain other countries. Cy 3 and Cy 5 are trademarks of Amersham Biosciences. RiboGreen is a registered trademark of Molecular Probes. www.stratagene.com 192-8510 9-1 0120-477-111 email_japan@agilent.com www.agilent.com/chem/jp Stratagene, an Agilent Technologies company, 2009 Printed in Japan, August 7, 2009 5990-3495JAJP Stratagene Products