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Neon Transfection System Invitrogen Neon Neon Transfection System Neon 10 4 5 10 6 DNA RNA Jurkat 細胞へのプラスミド導入結果 Neon システムを用いて Jurkat 細胞へ EGFP 遺伝子を導入後 24 時間でレポーター遺伝子の発現を観察した (A) A 位相差顕微鏡での細胞像 (B) B 蛍光顕微鏡での細胞像 ゲノム編集にも幅広く活用できます Neon システムは 一般的なトランスフェクション実験だけでなく 最近急速に広がっているゲノム編集技術においても大変有用なツールです リポフェクション試薬では導入が難しい細胞を用いたゲノム編集実験において さまざまなフォーマットの CRISPR の導入に活用いただけます ゲノム編集用トランスフェクションの選択 画期的なピペットチップ型電極 トランスフェクション 試薬および機器 Lipofectamine CRISPRMAX Lipofectamine 3000 CRISPR DNA CRISPR delivery methods CRISPR mrna CRISPR protein Lenti-Viral production 10 µl という極微量でのエレクトロポレーションが可能 従来のキュベットタイプと比べて Cas9 タンパク質のコストを大幅に削減できます Neon システム Lipofectamine MessengerMAX さまざまな種類の細胞で Neon を用いた Cas9 タンパク質によるゲノム編集の実績があります 参考論文 Biotechnology Letters, 2016 Jun ; 38(6): 919-29 Neon Neon Transfection System Starter Pack 1 1 MPK5000S 1,164,000 900,000 Neon Transfection System 2 1 MPK5000 1,000,000 780,000 Neon Neon Transfection System Kit 100 µl 1 kit Neon 96 MPK10096 192,400 1 kit Neon 25 MPK10025 64,200 Neon Transfection System Kit 10 µl 1 kit Neon 96 MPK1096 192,400 1 kit Neon 25 MPK1025 64,200 1 Neon Transfection System Starter Pack MPK5000S Neon Transfection System MPK5000 Neon Transfection Kit MPK10096 MPK1096 1 2 Neon Transfection System MPK5000 Neon Neon Pipette MPP100 Neon Pipette Station MPS100 RCAH28 thermofisher.com/jp-dls 11

Invitrogen LentiArray CRISPR Library Invitrogen LentiArray CRISPR Library 2 grna 4 grna 1 x 10 8 TU / ml 100 µl 50 µl 2 sgrna 発現用フォーマット LTR psi cppt PRE plenticrispr-efs-puro sgrna Scaffold Pol lll term U6 FES Puro Unique sgrna U6プロモーターと特異的 sgrnaを接続 EF-1aプロモーターとPuromycin 耐性遺伝子 (Puro) を接続 WPRE LTR Human Kinase CRISPR Library 100 µl 2,980,000 LentiArray Human Kinase CRISPR Library 822 A31931 A32167 LentiArray Human GPCR CRISPR Library 446 A31947 A32183 LentiArray Human Epigenetics CRISPR Library 396 A31934 A32170 LentiArray Human Phosphatase CRISPR Library 288 A31932 A32168 LentiArray Human DNA Damage Response CRISPR Library 516 A31946 A32182 LentiArray Human Cell Surface CRISPR Library 778 A31943 A32179 LentiArray Human Cancer Biology CRISPR Library 510 A31933 A32169 LentiArray Human Ubiquitin CRISPR Library 943 A31935 A32171 LentiArray Human Apoptosis CRISPR Library 904 A31940 A32176 LentiArray Human Tumor Suppressor CRISPR Library 716 A31945 A32181 LentiArray Human Transcription Factors CRISPR Library 1,817 A31938 A32174 LentiArray Human Cell Cycle CRISPR Library 1,444 A31936 A32172 LentiArray Human Drug Transporter CRISPR Library 98 A31941 A32177 LentiArray Human Ion Channel CRISPR Library 328 A31942 A32178 LentiArray Human Membrane Trafficking CRISPR Library 141 A31937 A32173 LentiArray Human Nuclear Hormone Receptors CRISPR Library 47 A31939 A32175 LentiArray Human Protease CRISPR Library 475 A31944 A32180 LentiArray Human Druggable CRISPR Library 10,132 A31948 A32184 LentiArray Human Whole Genome CRISPR Library 18,453 A31949 A32185 CRISPR CRISPR sirna TEL: 03-6832-9300 thermofisher.com/lentiarraylibraries 12 thermofisher.com/jp-dls

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