LTJ_NEXT_No4_ indd
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1 Life Technologies NEXT &5 No.4 CONTENTS NEW ROS CellROX Deep Red Reagent CellEvent Caspase-3/7 green detection IR & UV Applied Biosystems ArcturusXT Laser Capture Microdissection System BigDye PCR 1 BigDye Direct Cycle Sequencing Kit H Personal Genome Machine PGM SOLiD AB Library Builder P2 P3 P4 P5 P6 P7 PRODUCT REVIEW SOLiD SOLiD EZ Bead GIBCO FAQ INFORMATION TS P7 P8 P10 P12 P12 P12 P12
2 NEW ROS CellROX Deep Red Reagent ROS / 644/665 nmgfp 4 30 HCS ROS Plate cells CellROX Deep Red Hoechst (30) PBS3 CellROX HepG2Nefadozone 24CellROX CellROX Deep Red 5 M Hoechst 33342Image-IT DEAD Green viability stain 20 nm U-2 OSCell ROX 100 M menadione120 M CellROX DeepRed Reagent Hoechst PBSHCS CellROX Deep Red reagent H2-DCFDA Dihydrodichloro fluorescein diacetate DHE Dihydroethidium GFP CellROX Deep Red Reagent 5 X 50 l* C ,000 * 5X50 l 2.5mM in DMSO 5 M 125ml20 2
![80 70 60 50 40 30 20 CellEvent (+ PB) PB (+ CellEvent ) 10 0-10 -10-9 -8-7 -6-5 -4 Log [ Staurosporine ]](/docs-images/79/80074067/images/3-2.jpg "M CellEvent PrestoBlue PB Caspase-3/7staurosporine CellEvent Caspase-3/7 Green Detection Reagent 100 l**")
3 NEW CellEvent caspase-3/7 green detection Caspase-3/ HCS* BioProbe64 DEVDCaspase-3/7 DEVD HeLaCaspase HeLaCaspase-3/7 HeLa 7.5 µm CellEvent Caspase-3/7 Green Detection Reagent A0.5 µm staurosporine B4 staurosporine HeLa HeLaCaspase-3/7Staurosporine Caspase Normalized RFU CellEvent (+ PB) PB (+ CellEvent ) Log [ Staurosporine ] M CellEvent PrestoBlue PB Caspase-3/7staurosporine CellEvent Caspase-3/7 Green Detection Reagent 100 l** C ,000 **100 l ml MicroPlate1096well 100 l/well 384well 25 l/well
4 NEW IR & UV Applied Biosystems ArcturusXT TM Applied Biosystems ArcturusXT IR UV IR UV CapSure LCM Cap Cap PCR StepOnePlus PCR 3500/3500xl Applied Biosystems ArcturusXT ViiA 7 PCR 5500xl SOLiD ArcturusXT IR & UV 1 ARCTURUSXT-B ArcturusXT IR & UV 1 ARCTURUSXT-BF ArcturusXT IR & Enhanced UV 1 ARCTURUSXT-EB ArcturusXT IR & Enhanced UV 1 ARCTURUSXT-EBF 4
5 NEW BigDye! PCR1 BigDye Direct Cycle Sequencing Kit BigDye PCR PCR PCR1 PCR BigDye BigDye PCR 1 BigDye XTerminator Hi-Di Formamide BigDye Direct Cycle Sequencing Kit BigDye Direct Cycle Sequencing Kit POP-7 BigDye Terminator v3.1 Kit POP-7 BigDye Direct Cycle sequencing kit BigDye Direct Cycle sequencing kit BigDye Direct Cycle sequencing kit , ,000 44, , M13 M13-tailed primer M13 forward primer sequence: 5' TGTAAAACGACGGCCAGT 3' M13 reverse primer sequence: 5' CAGGAAACAGCTATGACC 3' 2 SAM XTerminator (BigDye XTerminator ) 5
6 NEW Ion Torrent -Personal Genome Machine PGM Ion Torrent Personal Genome Machine DNA H + Post Light TechnologyDNA The Chip is the Machine PGM : Ion Torrent DNA 2 PGM : Ion Chip DNA H+ H+ ph DNA2H Mbp1GbpRNA-Seq PGM RNA-Seq ChIP-Seq Ion 314 Chip, Ion 316 Chip, Ion 318 Chip Mbp/ Mbp/ Gbp/318* 200bp *99.99% PGM 200bp2 99.5% 100bp bp, bp denovo 1GB 100MB 10MB 100bp 200bp 400bp denovo QC RNA-Seq- ChIP-Seq 610* Web 6
7 NEW SOLiD AB Library Builder AB Library Builder SOLiD SOLiD SOLiDSOLiD SOLiD50% 5500 SOLiDSOLiD AB Library Builder ,800,000 AB Library Builder PRODUCT REVIEW SOLiD SOLiD EZ Bead SOLiD EZ Bead SOLiD 96 AB Library Builder Step 1 Step 2 PCR Step 3 EZ Bead Emulsifier EZ Bead Amplifier EZ Bead Enricher SOLiD EZ Bead ,200,000 7
8 PRODUCT REVIEW GIBCO FBS FBS50 90% DMEM/F12 RPMI FBSFBS 50%FBS MDBK, Hep-2, COS-7, A549, MDCK, WI-38, L- 110mg/L 1 5% cgmpiso9001 Advanced DMEM/F12 Advanced DMEM/F12 Advanced RPMI Advanced RPMI Advanced DMEM Advanced DMEM Advanced MEM Advanced MEM 500ml 500ml 500ml 500ml ,200 30,200 3,000 28,000 2,800 26,800 2,800 26,800 Recovery Recovery Cell Culture Freezing Media 25% DMSO Recovery Recovery medium) % (normalized to AE-1 CHO MDCK Swiss 3T3 HeLa x 10 4 / 6.0 x 10 5 / Recovery - Cell Culture Freezing Medium CCFM n 4 Cell type Recovery CCFM (Cat. no ) A B C D E F G Recovery Cell Culture Freezing Medium 50ml ,300 cgmp ES cgmp 10%DMSO HEPES Synth-a-Freeze 50ml A ,700 8
9 Sf-900 III GIBCO L- GIBCO Sf-900 III SFM Cells/ml Day 4 Sf-900 II, lot #1 Sf-900 II, lot #2 Sf-900 III, lot #1 Sf-900 III, lot #2 Sf-900 III, lot #3 Day 5 Day 6 Day 7 SF 900 III SFM SF 900 III SFM SF-900 III SFM SF9 CELLS ADAPTED IN SF900 III SF21 CELLS ADAPT IN SF900 III Cells/ml ml 1,000ml 6X1,000ml 1.5ml 1.5ml Lot #1 Lot #2 Lot #3 Lot #1 Lot #2 Sf-900 II Sf-900 III JP ,400 7,800 41,600 26,900 26,900 GIBCO Sf-9 Sf-21 Grace's Insect Cell Culture Medium Grace's Insect Cell Culture Medium 500ml 100ml ,400 3,700 Grace's Insect Cell Culture Medium 500ml ,500 Grace's Insect Cell Culture Medium 10X1L ,500 Grace's Insect Cell Culture Medium ,700 GIBCO GIBCO GIBCO bug bath NEW GIBCO DMEM DMEM RPMI1640 DMEM -MEM ,400 15,400 15,400 15,400 26,500 9
10 FAQ GIBCO 1962 Q A DMEM MEM RPMI16405%CO2 NaHCO3 CO2 MEM Earle srpmi1640 DMEM/F125%CO2 CO2CO2 ph CO2 CO2 DMEM 10%CO2 DMEM 5%CO2 15% CO2 ph 8 1 CO2 DMEM MEM, Earle s MEM, Hanks RPMI1640 DMEM/F g/l 2.2 g/l 0.35 g/l 2.0 g/l g/l CO2 10% 5% 1% 5% 5% Q A L-Glutamine L-Glutamine ATP L-Glutamine L-Glutamine L-Glutamine 30% 1-4 mm L-Glutamine 10 L-Glutamine GlutaMAX-I GlutaMAX-I L-AlanineL-Glutamine GlutaMAX-I 1 37GlutaMAX-I L-Glutamine mg/l L-glutamine GlutaMAX -I GlutaMAX -I L-glutamine Day 10
11 Q&A Support FAQ Q&A Q A Q A FBS FCS BS CS FBS Fetal Bovine Serum FCS Fetal Calf Serum BS Bovine Serum CS Calf Serum 2003 Calf Serum Calf Bovine Serum 2003 Calf Serum Invitrogen, Life Technologies, GIBCOBovine Serum Newborn Calf Serum, NBCS 14 FBS GIBCOFBS BS NBCS Horse Serum, HS 50% FBS NBCS BS HS GIBCO10FBS 2 Biochemical and Hormone Profile for Serum Biochemical Component Total Protein ph Osmolality Glucose Hemoglobin Bilirubin Uric Acid Urea Nitrogen Creatinine Sodium Potassium Calcium (total) Chloride Phosphorus (inorganic) Iron (total) Albumin Globulin (total) Alkaline Phosphatase GG-Transpeptidase SGOT Lactate Dehydrogenase Cholesterol Low Density Lipoprotein High Density Lipoprotein Triglycerides Growth Hormone Insulin Estradiol Progesterone Testosterone T4 (Thyroxine) T3 Units g/dl units mosm/kg meq/l meq/l meq/l g/dl g/dl U/l U/l U/l U/l ng/ml miu/ml pg/ml ng/ml ng/ml ng/ml Fetal Bovine Serum mean (range) Bovine Serum mean (range) Newborn Calf Serum mean (range) Horse Serum mean (range) not tested Q A GIBCO Grand Island Biological Company Life Technologies Corporation 11
12 INFORMATION TS TEL FAX jptech lifetech.com KKR http //jse2011.umin.jp/ EPIGENETICSTOOL USR&D http //jtca.dokkyomed.ac.jp/jtca/meet/y2011/ GIBCO QUIZ Life Technologies 10Life Technologies GIBCO Q GIBCO A Invitrogen Corporation Applied Biosystems Inc Invitrogen Applied Biosystems PCR DNA The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. 2011, Life Technologies Japan Ltd. All rights reserved. Printed in Japan TEL FAX
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