- PDF Free Download

14 2 5

20 21 DNA 1 1

() () 3

4 J.R.Webster, M.A.Burms, D.T.Burke and C.H.Mastrangelo Center for Integrated Microsystems, Department of Electrical Engineering and Computer Science, Department of Chemical Engineering, and Departmentof Human Genetics, University of Michigan, Ann Arbor, Michigan 48109-2122 Analytical Chemistry, Vol. 73, No. 7, April 1, 2001 AZO SYBR Green DNA LED SYBR-Green dsdna 0.9 cm 30,000 75 fg 7

Zhenhua Liang, Nghia Chiem, Gregor Ocvirk, Thompson Tang, Karl Fluri, and D. Jed Harrison Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2 Analytical Chemistry, Vol. 68, No. 6, March 15, 1996 U 120140m 10 6M 20 20,000 3nM 120m 50M 0.003auS/N3 Ar 2 FITC SN3 6M 3nM 20,000 PMT 8

Jorg Hubner, Research Center COM, Orsteds Plads, Building 345 West, Technical University of Denmark, DK-2800 Lyngby, Denmark Klaus B. Mogensen, Anders M. Jorgensen, Peter Friis, Pieter Telleman, and Jorg P. Kutter Mikroelektronik Centret, Orsteds Plads, Building 345 East, Technical University of Denmark, DK-2800 Lyngby, Denmark REVIEW OF SCIENTIFIC INSTRUMENTS Vol.72,No.1 Jan.2001 Bodipy650665X 250pMBodipy650665X 100nM 10000m 2 50m 4 m24m 1.2 500m 18dB 250pM 9

870W 10000m 2 9W/cm 2 Bodipy 100M Michael L. Chabinyc, Daniel T. Chiu, J. Cooper McDonald, Abraham D. Stroock, James F. Christian, Arieh M. Karger, and George M. Whitesides Department of Chemistry and Chemical Biology, Harvard University, Radiation Monitoring Devices, Inc., Analytical Chemistry, Vol. 73, No.18, September 15, 2001 PDMS APDmicroavalanche photodiode PDMS 100m APD PDMS (1cm) 80m APD APD 10

APD 1 (30m) (50m ADP 25nM) CE APD PDMS APDs APD Mantz Webster 4 ( ) 11

LED 488 nm LED 75 fg 3 nm 250 pm 25 nm (DNA 72bp) 20,000 ( ) ( ) 6M 100 nm FITC (Bodipy650/655X) 12

cm 2 Lambert-Beer Abs. log I I 0 = εcλ I0 I ε mol/lcm -1 c mol/l λ cm Lambert-Beer 10100µm 10-3 ε=10 4 mol/l -1 cm -1 10µmλ10-3 cm c=10-4 mol/l 13

LIF Laser Induced Fluorescence 1 1 DNA 1 14

Thermal Lens Microscope Radiative Transition Non-Radiative NMR 15

NMR NMR CCD 16

CCD 3 6G 514.5nm 6465nm 100µm 1kHz 17

1 2σ 2.710-7 M 3.010-7 M 2.710-8 M S/N=2 9.110-9 M 1.210-8 M 2σ S/N=2 100µm 40µm GRIN GRIN 19

70mm30mm1.4mm 3 200µm 100µm 3 20

2 1 2 SELFOC R IV 23

S M L S M L S M L 2 2 2 25

SELFOC R SML SELFOC R SELFOC R SELFOC R n0 2 g r nr r 2 nr n01 g 2 2r 2 SELFOC R Z0 0Z0 2g s0cotgz0 n0g SELFOC R SELFOC R 26

SELFOC R 1mm 2.3mm 40um 658nm 785nm SELFOC R SELFOC R SELFOC R SELFOC R SELFOC R SELFOC R 27

2 2 658nm 785nm 35mW ONOFF ONOFF 28

0 2.0kHz 0 10 40 658nm 4m 29

1 2 1 2 2 2 SELFOC R 14 SELFOC R 785nm 658nm SELFOC R SELFOC R 2 SELFOC R 658nm 30

SELFOC R SELFOC R SELFOC R 785nm SELFOC R SELFOC R SELFOC R 2 2 SELFOC R 2 SELFOC R 2 ONOFF 2.0kHz S1337-33BR 31

785nm 780FS2012.5 2 2.4mm 1mm 32

BNC IV NF LI5640 IV 0.01mA 3.3V IV IV IV 33

1 4 4 M5 X,Y X 34

Y Z Z Y Z Z Y X X,Y 35

KD Scientific Model-200 TASCO TMS-80A 1560 2090H 0.1 1H 2 36

2 2 2 37

50 100 TTL FC FC 38

658nm 785nm LD 35mW 35mW FC 26mWCW 1.23.5mW 0.41.5mW 0CW 1.2kHz CW DC9V 100W140D40H 0.5Kg SELFOC R SELFOC R 1mm 2.331mm SELFOC R 658nm 785nm 40m 39

SELFOC R SELFOC R db 18dB 3 50dB 0.001 40

0.5mm 3 41

4 4 Y 3nm 3mm 2 X,Y 6.5 42

3 ELISA γ 658nm 785nm 658nm 785nm Nickel(II) phtalocyanine tetrasulfonic acid, tetrasodium salt 1) 658nm 50000 658nm 660nm 7253-Diethylamino-7-diethyliminophenoxazonium Perchlorate 3 2 110-5 M 4 1kHz 45

2 2 1.95 1.9 1.95 1.9 1.85 1.85 1.8 1.75 1.8 1.75 1.7 1 2 3 4 5 1.7 1 3 5 7 9 100 3 2 S/N S/N 110-5 M S/N S/N S/N 1kHz S/N 1kHz 46

800 600 400 200 0 0 500 1000 1500 2000 2500 S/N S/N S/N 63µW 120 100 80 60 40 20 0 0 100 200 300 400 500 600 700 µ 47

2σ S/N 2 658nm 3 mol/l 1.0710-7 mol/l 6.5310-8 Abs 5.0410-5 Abs 3.2710-5 mol/l 7.7010-9 mol/l 6.3110-9 Abs 3.8510-6 Abs 3.1610-6 532nm 9mW 12mW mol/l 2.4110-8 1.1010-8 Abs 1.1210-6 5.1210-7 mol/l 1.8710-8 8.2710-9 Abs 8.2910-7 3.8510-7 48

1 3mW 13 14 SELFOC NA 0.2 NA 0.46 2σ S/N2 0 90 2 2σ S/N=2 0 90 mol/l 2.310-6 1.810-7 mol/l 8.710-7 3.610-7 49

90 0 2 2 6G 514.5nm 65058 0.95 725 658nm 61487 0.11 2 ELISA γifnγ γ ELISA 25µm 50

IFNγ IFNγ 1µl/min 5 10 1µl/min5 IFN-γ HRP 110-3 M a 110-3 M btmbz 110-4 M / 5mm 658nm IFNγ 0.010.1ng/ml IFNγ 0.03100ng/ml 0.11ng/ml TMBZ 4-AA TOOS 0.01ng/ml 0.03ng/ml 1 IFNγ 0.3 0.25 0.2 0.15 0.1 0.05 0 0.0001 C 0.001 0.01 0.1 1 IFN-γ (ng/ml) γ 51

1.5 1 0.5 0 0.0001 C 0.001 0.01 0.1 1 IFN-γ (ng/ml) γ γ 0.01ng/ml 52

IT MALDITOF 55

DNA NIST 56

µ µ 60

15 6 1 4 28 TEL03(3454)1311 3 22 TEL03(3292)0642