Fusarium head blightfhb, or scab, caused by several Fusarium species, is a widespread and destructive disease of wheat and barley. FHB resistance is difficult to evaluate because environmental conditions greatly influence FHB infection and development. To reduce environmental influence in such evaluations in barley, we developed a pot-plant method in which the spikes at the anthesis of barley varieties planted in plastic pots were spray-inoculated and hygrothermal conditions after inoculation were controlled to favor the pathogen s infection and development. Degrees of barley resistance in this method correlated highly with those in the cut-spike methodr0.770.82and field testsr0.680.74, and year-to-year correlations were highr0.790.89, suggesting that the pot-plant method is useful in accurate, stable FHB resistance evaluation in barley. : Fusarium head blight, scab, barley, resistance, testing method, pot-plant method.
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r0.770.82r0.680.74 r0.790.89 2 Ban, T. and K. Suenaga2000Genetic analysis of resistance to Fusarium head blight caused by Fusarium graminearum in Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165. Euphytica, 113, 87-99. 2002 56, 58-63. Gocho, H. and T. Hirai1987Varietal resistance to scab in barley. Barley Genetics, 625-630. 1962 1349, 177-188. 1993 23, 1-104. Kolb, F.L., G-H. Bai, G.J. Muehlbauer, J.A. Anderson, K.P. Smith and G. Fedak2001 Symposium on genetic solutions to Fusarium head blight in wheat and barley: challenges, opportunities, and imperatives. Crop Sci. 41, 611-619. Miedaner, T.1997Breeding wheat and rye for resistance to Fusarium diseases. Plant Breeding
116, 201-220. 1964 1 10, 146-170. 1958 97, 1-162. Parry, D. W., P. Jenkinson and L. McLeod1995 Fusarium ear blightscabin small grain cereals-a review. Plant Pathology 44, 207-238. Rudd, J.C., R.D. Horsley, A.L. McKendry and E.M. Elias2001Host plant resistance genes for Fusarium head blight: Sources, mechanisms, and utility in conventional breeding systems. Crop Sci. 41, 620-627. 1989 39, 203-216. 1995 45, 349-356. 2000 2 2, 170. 2002 4 1, 226.
The pot-plant method provides accurate, stable testing of the resistance to Fusarium head blight in barley. Megumi YOSHIDA, Naoyuki KAWADA and Takuji TOHNOOKA Fusarium head blightfhb, or scab, caused by several Fusarium species, is a widespread and destructive disease of wheat and barley. FHB resistance is difficult to evaluate because environmental conditions greatly influence FHB infection and development. To reduce the environmental influence in such evaluations in barley, we developed a pot-plant method in which the spikes at the anthesis of barley varieties planted in plastic pots were spray-inoculated and hygrothermal conditions after inoculation were controlled to favor the pathogen s infection and development. Degrees of resistance of barley varieties in this method correlated highly with those in the cut-spike method r0.770.82and field testsr0.680.74, and year-to-year correlations were high r0.790.89, suggesting that the pot-plant method is useful in accurate, stable evaluation of FHB resistance in barley. We study and compare characters of the pot-plant method and other testing methods. Received 15 January, 2003 National Agricultural Research Center for Kyushu Okinawa Region