06-08 SYBR Green Realtime PCR Master Mix -Plus- (Code No. QPK-211, 211T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN
(2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc. The Perkin-Elmer Corporation Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process for The Research Field in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorized thermal cycler. LightCycler TM Idaho Technology Inc. Roche Molecular Systems Inc. SYBR Molecular Probes Inc. ABI PRISM The Perkin-Elmer Corporation - - 1 - -
PCR SYBR Green Realtime PCR Master Mix -Plus- dntp PCR SYBR Green I DNA PCR LightCycler TM BSA ABI PRISM 7700 LightCycler TM LineGene Taq PCR 10 1 LightCycler TM PCR - - 2 - -
-20 10 1 4 (QPK-211 1-20 4 ) [SYBR Green Realtime PCR Master Mix -Plus-] dntp MgCl 2 Taq DNA Taq PCR SYBR Green I 2 - - 3 - -
[ ] PCR 20 30mer GC 40 60% 200bp 150bp 50 150bp [ ] PCR [ PCR ] ABI PRISM 7700 LightCycler TM LineGene PCR DNA [cdna] 1st strand cdna 1st strand cdna dt PCR 1st strand cdna ReverTra Ace - - (Code:FSK-101) 10 cdna ( ) PCR [ DNA ] PCR DNA 1 10ng DNA - - 4 - -
ABI PRISM 7700 (1) [PCR ( )]( 0.4 M 50 L) 16 L SYBR Green Realtime PCR Master Mix -Plus- 25 L 1 (10 M) 2 L 2 (10 M) 2 L 5 L 50 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) 0.2 0.6 M (2)PCR 3 ( 1) Tm 55 65 60 15 PCR 1 5 Detector SYBR Quencher None PCR data collection 30 2 ( 2) data collection - - 5 - -
[PCR ( 1)](3 60 72 ) 95 60 95 15 40 60 15 72 45 (data collection) [PCR ( 2)](2 60 ) 95 60 95 15 40 60 60 (data collection) - - 6 - -
Roche LightCycler TM (1) [PCR ( )]( 0.6 M 20 L) 5.6 L SYBR Green Realtime PCR Master Mix -Plus- 10 L 1 (10 M) 1.2 L 2 (10 M) 1.2 L 2 L 20 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) 0.4 0.8 M (2)PCR Tm 55 65 55 10 20 5 30 5 PCR 1 5 200bp 15 data collection 10 ( ) Detector F1 Temperature Transition Rate 20 /sec( ) PCR Temperature Transition Rate 2 /sec 2 data collection - - 7 - -
[PCR ( )](3 55 72 ) 95 30 95 5 40 55 10 72 15 (data collection) (Melting Curve) - - 8 - -
BioFlux LineGene (1) [PCR ( )]( 0.4 M 10 L) 2.2 L SYBR Green Realtime PCR Master Mix -Plus- 5 L 1 (10 M) 0.4 L 2 (10 M) 0.4 L 2 L 10 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) 0.2 0.6 M (2)PCR 3 Tm 55 65 60 15 PCR 1 5 Channel F1 2 data collection [PCR ( )](3 60 72 ) 95 60 95 15 40 60 15 72 30 (data collection) (Melting Curve) - - 9 - -
(1) ( ) SYBR Green I (2) PCR PCR GC cdna (1) ( ) (2) cdna - - 10 - -
PCR PCR GC (1) PCR ( ) (2) ( ) PCR 3 - - 11 - -
Code No. 1st cdna ReverTra Ace - - ( dntps ) 100FSK-101 ReverTra Ace 10,000U 1 50,000U 1 TRT-101 TRT-102 RNase Inhibitor (Native type) 2,500U 1 2,500U 5 SIN-101 SIN-102 RNase Inhibitor (Recombinant type) 2,500U 1 2,500U 5 SIN-201 SIN-202 Realtime PCR Master Mix 1mL 1 QPK-101T 1mL 5 QPK-101 SYBR Green Realtime PCR Master Mix 1mL 1 QPK-201T 1mL 5 QPK-201 1 BFFQD-33A LineGene ( ) - - 12 - -
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