www.dako.jp IHC Solution Visualization Systems Number of Steps 5 4 3 2 1 LSAB TM 2 EnVision TM G 2 Doublestain EnVision TM DuoFLEX Doublestain LSAB TM + EnVision TM G 2 EnVision TM + EnVision TM FLEX 1 2 3 4 5 6 Comparative Features of Dako Visualization Systems Sensitivity EnVision TM FLEX+ Product Name No. of Steps Primary Antibody Primary Antibody Enzyme Label Technology EnVision TM FLEX 2 Concentrate or FLEX RTU Mouse + Rabbit HRP Dextran (Biotin-Free) EnVision TM FLEX+ 3 Concentrate or FLEX RTU Mouse + Rabbit HRP Dextran (Biotin-Free) EnVision TM + 2 Concentrate Mouse and/or Rabbit HRP Dextran (Biotin-Free) EnVision TM DuoFLEX Doublestain CSA II 4 Cocktail or Concentrate Mouse + Rabbit AP and HRP Dextran (Biotin-Free) EnVision TM G 2 3 Concentrate or RTU Mouse + Rabbit AP Dextran (Biotin-Free) EnVision TM G 2 Doublestain 5 Concentrate or RTU Mouse + Rabbit AP and HRP Dextran (Biotin-Free) CSA 5 Concentrate Mouse or Rabbit HRP Tyramide CSA II 4 Concentrate Mouse or Rabbit HRP Tyramide (Biotin-Free) LSAB TM + 3 Concentrate Mouse + Rabbit + Goat CSA AP or HRP Labeled Streptavidin-Biotin LSAB TM 2 3 Concentrate or RTU Mouse + Rabbit AP or HRP Labeled Streptavidin-Biotin 106 : (9) : :
EnVision FLEX EnVision TM FLEX, High ph Link Envision FLEX High ph AutostainerLink D K8000 HRP 400-600 tests EnVision TM FLEX Mini Kit, High ph Link Envision FLEX Mini Kit High ph AutostainerLink D K8023 HRP 125-190 tests EnVision TM FLEX Dako EnVision TM FLEX EnVision TM FLEX FLEX EnVision TM FLEX, High ph Dako Autostainer/Autostainer Plus Envision FLEX High ph Autostainer/Autostainer plus D K8010 HRP 400-600 tests EnVision TM FLEX Mini Kit, High ph Dako Autostainer/Autostainer Plus Envision FLEX Mini Kit High ph Autostainer / Autostainer plus D K8024 HRP 125-190 tests Visualization Systems IHC Solution K8000 K8010 K8023 K8024 3 x 40 ml 10 x 13 ml 1 x 40 ml 3 x 13 ml 3 x 40 ml 10 x 13 ml 1 x 40 ml 3 x 13 ml 3 x 3 ml 3 x 3 ml 1 x 3 ml 1 x 3 ml 12 x 20 ml 20 x 13 ml 5 x 20 ml 7 x 13 ml 9 x 30 ml 9 x 30 ml 3 x 30 ml 3 x 30 ml 4 x 1 L 4 x 1 L 2 x 1 L 2 x 1 L TEL : 03-5802-2583 FAX : 03-5802-2584 107
EnVision FLEX Envision FLEX Antibody Diluent Envision FLEX K8006 400-600 tests, 120 ml Rabbit LINKER Envision FLEX+AutostainerLink K8009 130-200 tests, 40 ml www.dako.jp IHC Solution Visualization Systems Hematoxylin Link Envision FLEX AutostainerLink K8008 AutostainerLink 400-600 tests, 3 x 45 ml Hematoxylin Dako Autostainer/Autostainer Plus Envision FLEX Autostainer / Autostainer plus K8018 400-600 tests, 10 x 13 ml Mouse LINKER Envision FLEX+ AutostainerLink K8021 130-200 tests, 40 ml Mouse LINKER Envision FLEX+ Autostainer / Autostainer plus K8022 120-190 tests, 3 x 13 ml Rabbit LINKER Envision FLEX+Autostainer / Autostainer plus K8019 120-190 tests, 3 x 13 ml Target Retrieval Solution, High ph Envision FLEX High ph 50 x K8004 50 3 x 30 ml Target Retrieval Solution, Low ph Envision FLEX Low ph 50 x K8005 50 3 x 30 ml Wash Buffer Envision FLEX 20 x K8007 20 1 L 108 : (9) : :
EnVision TM DuoFLEX 2 mouse STEP 1 Application of mouse and rabbit primary antibodies. rabbit mouse rabbit STEP 2 Application of HRP-conjugated polymers and secondary polymers. EnVision TM DuoFLEX Doublestain System EnVision TM DuoFLEX SK110 HRP/AP, Rabbit/Mouse 100-150 tests, 30 ml K6807 HRP/AP, Rabbit/Mouse 100-150 tests, 30 ml EnVision TM DuoFLEX Autostainer Link (SK110) Autostainer K6807 DAB+ (HRP) AP) EnVision TM DuoFLEX Duo FLEX 1 200µL 300µL mouse rabbit STEP 3 Application of AP-conjugated polymers. DAB mouse DAB LPR rabbit LPR STEP 4 Application of chromogenic substrates: brown DAB and LPR. Visualization Systems IHC Solution TEL : 03-5802-2583 FAX : 03-5802-2584 109
EnVision 2 www.dako.jp IHC Solution Visualization Systems Step 1. Step 2.EnVision TM EnVision TM G 2 Doublestain System Rabbit/Mouse DAB+/Permanent Red EnVision G 2 DAB+/ K5361 150 tests 1 2 150 Vial 1: 11mL x 3 Vial 2: 11mL x 3 Vial 3: DAB+ 11mL x 3 Vial 4: DAB+ 11mL x 3 Vial 5: 11mL x 3 Vial 6: 11mL x 3 Vial 7: 11mL x 3 Vial 8: 11mL x 3 Vial 9: 300L x 1 EnVision TM G 2 System/AP Rabbit/Mouse Permanent Red EnVision G 2 / AP K5355 50 tests/500 tests EnVision TM + Kits EnVision TM + System / HRP, Mouse (DAB+), RUO K4006 150 tests K4007 1100 tests ABC K4006 K4007 0.03 15 ml x 1 110 ml x 1 15 ml x 1 110 ml x 1 18 ml x 1 120 ml x 1 3,3'- DAB 1 ml x 1 5 ml x 1 DAB+ K3467 15 ml 3,3'- K3468 110 ml EnVision TM + Dual Link, Single Reagents EnVision TM + K4063 HRP 150 tests, 15 ml K4061 HRP - Autostainer 1100 tests, 10 x 11 ml ENVISION Autostainer 10 11mL 15mL 2 K4063 K4061 15 ml 11 ml x 10 110 : (9) : :
EnVision TM + Single Reagents EnVision TM + / HRP, RUO K4000 150 tests, 15 ml K4001 1100 tests, 110 ml K4002 150 tests, 15 ml K4003 1100 tests, 110 ml 2 K4000 K4001 50 15 ml 110 ml K4002 K4003 15 ml 110 ml EnVision TM Detection System EnVision TM Detection System / HRP, Rabbit/Mouse (DAB+), RUO K5007 500 tests Autostainer/Autostainer plus DAB K5007 : 100 ml A : 100 ml x 1 B : 250 ml x 1 C : 3,3'- DAB 5 ml x 1 Visualization Systems IHC Solution TEL : 03-5802-2583 FAX : 03-5802-2584 111
CSA LSAB sabc CSA System www.dako.jp IHC Solution Visualization Systems 112 Step 1. Step 2. Step 3. - CSA, Catalyzed Signal Amplification System* CSA HRP DAB K1500, 150 tests ABC Step 1. Step 2. CSA II, Biotin-Free Catalyzed Amplification System* CSA II K1497 150 tests, 15 ml CSA II FITC FITC DAB LSAB ABC CSA II CSA Code No. K1500 : (9) : : Step 4. Step 5. K1500 1 3 15 ml x 1 2 15 ml x 1 15 ml x 1 A 1 ml x 1 B 1 ml x 1 PBS 25 ml x 1 15 ml x 1 15 ml x 1 DAB 10 x 1 6 ml x 1 0.8 2 ml x 1 x 1 x 1 x 1 Step 3.FITC Step 4. FITC K1497 1 3 15 ml x 1 2 15 ml x 1 15 ml x 1 FITC 15 ml x 1 FITC 15 ml x 1 18 ml x 1 3,3 1 ml x 1 CSA II Rabbit Link CSA Rabbit Link K1501 150 tests, 15 ml CSA II Rabbit Link CSA II Code No. K1497 CSA II Code No. K1497
LSAB TM Kits Step 1. Step 2. Step 3. LSAB TM + Kits, Universal LSAB TM + K0678 AP K0679 HRP K0690 HRP 150 tests 150 tests 1100 tests HRP K0679 K0690 3 15 ml x 1 15 ml x 1 110 ml x 1 15 ml x 1 110 ml x 1 18 ml x 1 3, 3'- 1 ml x 1 AP K0678 15 ml x 1 15 ml x 1 2 ml x 1 2 ml x 1 30 ml x 1 x 1 1 ml x 1 LSAB TM 2 Kits, Universal LSAB TM 2 Kits, Universal / HRP, Rabbit/Mouse, RUO K0675 HRP 1100 tests K0609 HRP 150 tests K0675 LSAB TM K0609 K0675 110mL x 1 110mL x 1 K0609 15 ml x 1 Bovine 15 ml x 1 Streptavidin Streptavidcin, RUO P0397 1 ml Streptomyces avidinii P0397 1:300 1:1,000 in situ 1:300 1:1,000 W.Blot. 1:3,000 1:9,000 ELISA 1:1,000 1:15,000 Visualization Systems IHC Solution TEL : 03-5802-2583 FAX : 03-5802-2584 113
IHC IHC www.dako.jp IHC Solution Ancillaries for IHC in situ hybridization AEC Substrate-Chromogen AEC Substrate-Chromogen, Ready-to-use, RUO K3464 1100 tests, 110 ml in situ hybridization Code No. C0563 Ultramount Mounting Medium Code No. S1964 K3464 AEC 0.75mg/mL ------ 15mL or 110mL * 2 8 AEC+ Substrate-Chromogen AEC+ 3- -9- K3461 150 tests, 15 ml K3469 1100 tests, 110 ml in situ hybridization AEC Code No. C0563 Ultramount Mounting Medium Code No. S1964 K3461 AEC 15mL 1 3-Amino-9-Ethyl Carbazole, K3469 AEC 110mL 1 3-Amino-9-Ethyl Carbazole, 2 8 8 2 8 2 8 CSA System Code No. K1500 * BCIP/NBT Substrate System BCIP/NBT K0598 150 tests in situ Hybridization [ ] BCIP/NBT 15mL * 2 8 1. 2. BCIP/NBT 3. 10 * BCIP/NBT 0.1 1mmol/L Code No. X3021 DAB+, Liquid DAB+, Liquid, RUO K3467 150 tests, 15 ml K3468 1100 tests, 110 ml in situ hybridization DAB K3467 K3468 DAB 3,3'-Diaminobenzidine Tetrahydrochloride 1mLx1 5mLx1 /ph7.5 15 ml x 1 110mLx1 1mL DAB 1 20µL 2 8 CSA System Code No. K1500 114 : (9) : :
IHC Fuchsin+ Substrate-Chromogen K0625 300 tests, 30 ml/1100 tests, 110 ml in situ hybridization Code No.K0698 110mL 30mL 0.5 + 7mL 2mL 7mL 2mL 110mL 30mL 1 1 20 1. 3 3 1 2. 1 2mL 3 2 2mL Levamisole Code No. X3021 2 New Fuchsin Substrate System K0698 1750 tests, in-situ hybridization,, Code No. X3021 2.5mol/L ph8.2 7mL 5mL 0.5% 5mL 5mL 2 8 1. 2.5mol/L ph8.2 2mL 2. 1 3. 0.5% 3 4. 1, 2 a+b 3 4 2mL Levamisole Code No. X3021 2 1. 2. 3. 10 20,, Ancillaries for IHC IHC Solution Permanent Red Substrate-Chromogen, Liquid K0640 300 tests, 30 ml/1100 tests, 110 ml in situ hybridization Texas Red 110mL 30mL 0.5 + 3mL 1mL 110mL 30mL 30 3mL 1. 2. 3mL 1 3. TEL : 03-5802-2583 FAX : 03-5802-2584 115
IHC www.dako.jp IHC Solution Ancillaries for IHC Antibody Diluent S0809 50 ml/125 ml ph, ph,, 15mmol/L 50mL, 125mL Antibody Diluent, Dako REAL TM Dako REAL TM S2022 250 ml Antibody Diluent, Background Reducing S3022, Tween 50 ml/125 ml ph, ph,,, 0.1% Tween, 15mmol/L Tween Biotin-Blocking System X0590 15 ml + 15 ml - LSAB LAB sabc ABC,, * 2 0.3mol/L 263mg 15mmol/L 0.1% 15mL 15mmol/L 0.01% 15mL * 2 8 * 0.3mol/L 263mg STEP 1 :, 10 TBS PBS TBS PBS 5 STEP 2 :, 10 TBS PBS TBS PBS 5 Levamisole Solution X3021 15 ml in situ hybridization Marginal Zone B Cell 1mL * 2 8 Peroxidase-Blocking Solution, Dako REAL TM Dako REAL TM S2023 250 ml 116 : (9) : :
IHC Phosphate-Buffered Saline, ph 7.0 0.15mol/L NaCl 20mmol/L ph7.0 S3024 6 x 1 L, in situ hybridization NaH 2 PO 4, Na 2 HPO 4, 6 * 1 1L * 0.02mol/L, 0.15mol/L, ph 7.0 * 2 8 Protein Block, Serum-Free X0909, 110 ml,,,, 0.25%,, 15mmol/L * 2 8 X0909 5 10 Target Retrieval Solution Target Retrieval Solution S1699 10 500 ml, 10x concentrated S1700 500 ml, in-situ hybridization DNA RNA ph6.1 95 99 * S1699 10 10 S1700 * 2 8 Target Retrieval Solution, ph 9 Target Retrieval Solution ph 9 S2367 10 500 ml, 10x concentrated S2368 500 ml 10mmol/L, ph6.0 ph 1mmol/L EDTA 10mmol/L, ph 9.0 TE Target Retrieval Solution, ph 9.0 Code No. S2367/S2368 TE S2367 10 10 S2368 Target Retrieval Solution, ph 9 10x, 3-in-1 Target Retrieval Solution ph 9, 10x, 3-in-1 S2375 10 500 ml, 10x concentrated Dako Target Retrieval Solution, ph 9, 10x, 3-in-1 Autostainer Link Dako PT Link Code No. PT100 1 Tris-Buffered NaCl Solution with Tween 20, ph 7.6 0.1%Tween 20 0.3mol/L NaCl 0.05mol/L ph7.6 10x S3306 10 500 ml, 10x concentrated, in situ hybridization CSA System Code No. K1500 GenPoint TM System Code No. K0620 Tween 20 10 * 2 8 * 0.05mol/L, 0.3mol/L, Tween 20 0.1%, ph 7.6 Ancillaries for IHC IHC Solution Target Retrieval Solution, Citrate ph 6 REAL TM S2031 10 500 ml, 10x concentrated ph 6.0 10 10 TEL : 03-5802-2583 FAX : 03-5802-2584 117
IHC www.dako.jp IHC Solution Ancillaries for IHC Tris-Buffered Saline, ph 7.6 0.15mol/L NaCl 0.05mol/L ph7.6 S3001 6 x 1 L S1968 2 x 5 L, in situ hybridization S3001,, 6 S1968,, 2 S3001 1 1L S1968 1 5L * 0.05mol/L, 0.15mol/L, ph 7.6 * 2 8 Tween 20 Tween20 Autostainer S1966 Autostainer 100 ml Autostainer Tween 20 Code No. S3006 Tween 20 Wash Buffer 10x 0.05% Tween20 0.15mol/L NaCl 0.05mol/L ph7.6 10x S3006 10 1 L, 10x concentrated 118 : (9) : :
IHC Dako REAL TM Hematoxylin Dako REAL TM S2020 500 ml Methyl Green S1962 500 ml Fluorescence Mounting Medium S3023 15 ml 2 8 1 1.47 1.50 Glycergel Mounting Medium, Aqueous C0563 15 ml * 2 8 1.44 1.47 Ultramount Permanent Mounting Medium, Aqueous S1964 15 ml DAB AEC Fast Red 1. 2. 3 4 3. 4. 2 3 /overnight 60 70 30 Ancillaries for IHC IHC Solution TEL : 03-5802-2583 FAX : 03-5802-2584 119
IHC in situ hybridization www.dako.jp IHC Solution Ancillaries for IHC Pepsin S3002 6 x 250 to 500 ml, in situ hybridization 1 500mL 0.2mol/L in situ hybridization 1 250mL 0.2mol/L * 20 37 37 10 30, in situ hybridization Dako Pen S2002 1 unit, " ", Silanized Slides S3003 100 slides, in-situ hybridization Triethoxyaminopropylsilane,, * Hydrophilic Silanized Slides Proteinase K K S3004 2mL S3020 150 tests, 15 ml/1100 tests, 110 ml, in situ hybridization 20mg/mL K * K 2 8 K 0.4mg/mL K 40µL 0.05mol/L ph7.5 7.7 2mL in situ hybridization K 0.8mg/mL K 80µL 0.05mol/L ph7.5 7.7 2mL * K 20 2mL K 6 15, in situ hybridization S30200.4mg/mL 6 15 in situ hybridization Hydrophilic Silanized Slide S4103 100 slides 120 : (9) : :
Universal Label Printer Link DL412 1 unit Autostainer Link, Artisan Link 3,000 100-240V, 50-60Hz 2.1kg 190mm x 260mm x 190mm Labeling Systems IHC Solution Autostainer Link Artisan Link Slide Label Kit, Large Flap S3417 3000 labels Autostainer Link 1 3,000 Slide Label Kit, Small Flap DL213 1500 labels Artisan Link 1 1,500 TEL : 03-5802-2583 FAX : 03-5802-2584 121