CT MRI CSFCSF CSF CSF CSF CSF CSF (cisternal puncture) CSF 20-23G 90 (20-23G) : CSF <0.2ml/kg CSF
5 CSF 6 CSF 7 CSF CSF CDV CDV GFAP CSF Pandy test NSE MBP LDH 6.2 16.3 mu/ml 0-5 /µl AST 3.4 26.3 mu/ml CK 0 69.7 mu/ml <25 mg/dl<45 mg/dl GLU 34 140.9 mg/dl Pandy test Cl - 122.3 134.7 meq/l 1.004-1.006 Ca 2+ 2.5 3.7 meq/l ph 7.3-7.4 Albumin 10-20 mg/dl NSE 3.9 21.8 ng/ml <1.4 cmh 2 O MBP 0 1.3 ng/ml (n=30) 14
0µL 50µL Pandy test50µl 50µL 200-300µL 500µL CDVFCoV 100-500µL GFAP 200µL NSE300µL MBP600µL HVA ph50µl 100µL LDH10µL AST10µL CK 10µL Glu10µL Cl - 30µL Ca 2+ 10µL Albumin500µL CSF 30 CSF
/ / I D y m M F ( ) mg/dl <25 mg/dl Pandy test 1.004-1.006 ph 7.3-7.4 WBC /µl RBC /µl M= % N= % Ly= % Eo= % Bas= % LDH mu/ml 6.2-16.3 AST mu/ml 3.4-26.3 CDV CK mu/ml 0-69.7 Glucose mg/dl 30-140.9 Glucose mg/dl CSF/ % Cl - meq/l 122.3-134.7 Ca 2+ meq/l 2.5-3.7 Albumin mg/dl 10-20 NSE ng/ml 3.9 21.8 MBP mu/ml 0 1.3 CDV S-100 HVA mu/ml 98.212.4 CDV CSF: FIP CSF: GFAP Version
CSF CSF ()(10h15days) CSF () >500/µL CSF 30 CSF Fuchs-Rosenthal Neubauer Neubauer Deep 1/10mm 1mm A C B D (A+B+C+D) 4 10 = (mm 3 )(µl) 1mm CSF 500 1 100 1 WBC RBC 0-5/µL 0-3/µL CSF Cytospin 3mL 5mL CSF CSF 0.30.5mL 2530 Wright-Giemsa ()
Cytocrip Slide Clip Cytocrip Slide Clip 100µL CSF Sample Chamber 750rpm64G 5 Wright-Giemsa Chamber Cytospin 1000rpm 55G 11% 48! GME FIP
CSF <25 mg/dl<45 mg/dl Pandy test 30mg/dL 1) 23mL 2) CSF 12 3) 3 4) 1.004-1.006 ph ph ph 7.3-7.4 34 ( S.intermedius)PCR 200mmH 2 O CSF CSF CSF 1.3cmH 2 O=mmHg <1.4cm H 2 O
CSF CSF CSF LDH ASTCKGLU(Na C1 - Ca 2+ ) (LDH) LDH CSF LDH CSF LDH 510% 90% 10% CDV LDH LDH LDH CSF ;(LDH2.3.4 ) 6.2-16.3 mu/ml (12-55mU/mL; Abate et al.) () LDH-1 LDH-2 LDH-3 LDH-4 LDH-5 47.3±7.3 28.8 3.6 13.7±2.6 4.8±2.0 9.0±7.8 (%) Mean±S.D. (n=30) (AST) AST CSF AST Abate CDV AST CDV AST CDV MRI AST 3.4-26.3 mu/ml (9-46 mu/ml; Abate et al.) CDV (CK) CK M() B()CSF CK MRI
150 mu/ml CSF CK CK (100 150mU/mL) CK-BB 0-69.7 mu/ml CDV (GLU) CSF GLU 60-80CSF GLU CSF GLU MRI CDV CSF GLU MRI CDV CSF GLU CSF 34-140.9 mg/dl (50-105 mg/dl; Benjamin) 60-80% (Cl - ) CSF Cl - Donan 1/3 CSF Cl - Cl - Cl - CSF Cl - CSF CSF CSF Cl - Cl - MRI 130meq/L Cl - 122.3-134.4 meq/l (131-138 meq/l; Feldman)
(Ca 2+ ) Ca2 2+ CSF Ca 2+ CSF 2.5-3.7 meq/l BBB CSF 10-20 mg/dl; Sarjoner BBB CSF CSF CSF (NSE)NSE S-100 (MBP) CDV MBP (NSE) NSE γ 1% NSE CSF NSE MRI NSE
3.9-21.8 ng/ml (<10 ng/ml: Hara et al.) (MBP) MBP CSF MBP CDV CDV CSF MBP MBP CDV CDV MRI MBP 0-1.3 ng/ml CDV S-100 S-100 S-100 CSF S-100 2 Creutzfeldt-Jakob HVA HVA 98.2912.42; Vaughn, DM.? CSF GFAP GFAP CSF GFAP CSF GFAP 90%
CSF CDV CDV CDV CDV CDV CSF CDV CDV CSF CDV CSF CDVCDV CDV CDV CDV CSF CDV CDV CDV CSF IgG 6 CSF IgG / IgG IgG CSF / CDV RT-PCR RT-PCR CDV CSF CDV RT-PCR CSF CDV CSF CSF (mg/dl) (g/dl)10 CDV CDV 1mL PCR IgG IgM CSF CSF 0.1mL 0.1mL IP ELISA CSF ELISA CSF RT-PCR IDEXX Laboratories CDV IgM IgG PHA CDV IgG IgM PHA IgGM CSF 0.3mL 0.3mL 0.3mL 0.5mL ELISA RT-PCR ELISA ELISA PHA RT-PCR RT-PCR PCR ELISA PHA ELISA
CSF FIP FCoV FIP FIP FIP FCoV FCoV FIP FIP FIP FCoV FIP FCoV FIP FCoV FIP FCoV FCoV FIP CSF FCoV CSF FCoV FIP FCoV CSF FCoV FIP CSF FCoV FCoV CSF 0.1mL ELISA 0.3mL PCR IPA IFA IDEXX Laboratories IPA CSF / CSF CSF 250300pg/ml 80 pg/ml MRI 250-300 pg/ml < 80pg/ml
CSF Pandy 1. O1iver, EJ., Lorenz, MD. and Kornegay, JN. Handbook of Veterinary NeurologyW.B Saunders Co., Philadelphia1997. 2. Nelson, RW., Couto, CG.. Small Animal Interna1 Medicine, Mosby, St Louis, 1998. 3. Delahunta, AD. Veterinary Neurology and Clinical Neurology. W.B.Saunders Co., Philadelphia, 1983. 4..... 1998. 5.,,.... 1998. 6.. Dr. Bagley... 2008. 7. Vandevelde, M. and Spana, J.S. Cerebrospinal fluid cytology in canine neurologic disease. Am. J. Vet. Res. 38(11):1827-1831.1977. 8. Abate, O., Bollo, E., Lotti, D.and Bo, S. Cytological, immunological and biochemical cerebrospinal fluid investigations in selected central nervous system disorders of dogs. J. Vet. Med. B, 45:73-85. 1998. 9. Summers, BA., Whitaker, JN. and Appe1, MJ. Demyelinating canine distemper encephalitis: measurement of myelin basic protein in cerebrospinal fluid. J. Neuroimmunol. 14(2):227-233. 1987. 10.,,, NSE S-100.52(2):141-147.1977. 11.,,,. Neuron Specific Enolase: NSE NSE.. 5:3-14. 1998. 12. Vandeverde, M., Kristensen, F., Kristensen, B., Steck, A.J. and Kihm, U. Immunological and pathological findings in demyelinating encephalitis associated with canine distemper virus infection. Acta. Neuropathol, 56(1):1-8.1982. 13. Vaughn, DM., Coleman, E., Simpson, ST., Whitmer, B. and Satjawatcharaphong, C. A rostrocaudal gradient for neurotransmitter metabolites and a caudorostal gradient for protein in canine cerebrospinal fluid. Am. J. Vet. Res. 49(12):2134-2137.1988. 14... InfoVets. 10(11):4-8. 2007. 15... InfoVets. 10(11):8-12. 2007. 16... InfoVets. 10(11):13-21. 2007.