Agilent Infinity ELSD Agilent 6 Infinity ELSD Agilent 9 Infinity ELSD 6 8 ELSD 9 LC/MS ELSD
Agilent Infinity ELSD ELSD ( ) ( ºC ) W x D x H mm LED ELSD ELSD ELSD UV UV LC/MS RSD % Column: Agilent Pursuit C8, x.6 mm, µm Eluent: Water: Acetonitrile :6 Flow rate:. ml/min Injection volume: µl ELS detection: neb. = ºC, evap. = ºC, gas flow =. SLM Sample: Tertiary aminols Column: C8, x.6 mm, µm Eluent A: Water +. % Formic acid Eluent B: Acetonitrile +. % Formic acid Gradient: 9 % B in min Flow rate:. ml/min Injection volume: µl ELS detection: neb. = ºC, evap. = ºC, gas flow =. SLM
ELSD ELSD UV ELSD. Prednisone. Dexamethasone Column: Agilent Polaris C8, x.6 mm, µm Eluent: Water: Acetonitrile : Flow rate:. ml/min Injection volume: µl ELS detection: neb. = ºC, evap. = ºC, gas flow =. SLM UV detection: 8 nm Agilent ELSD UV, 8 nm RI RI Sample: Carbohydrates Column: Hi-Plex Ca, x mm Eluent: Water Flow rate:.6 ml/min Temperature: 8 ºC Injection volume: µl. Melezitose. Maltose. Glucose. Mannose. Fructose 6. Ribitol (adonitol) 6 Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM Agilent ELSD RI Detector LC/MS : MS LC/MS MS ELSD Column: Eluent A: Eluent B: Flow rate: Injection volume: µl Pursuit XRs C8, x mm, µm. % formic acid in water. % formic acid in acetonitrile. ml/min. ( pg)
ºC FAME C6 FAME ELSD Agilent ELSD ºC (C) Sample: Fatty Acid Methyl Esters mix (C8 to C) Column: C8, x. mm, µm Eluent A: Acetonitrile Eluent B: Dichloromethane Isocratic: 7/ A/B Flow rate:. ml/min Injection volume: µl Detection: neb. = 8 ºC, evap. as shown, gas flow =.6 SLM C6: C6: C C8 C9 C C C6: C8 C ELSD C ELSD C UV RI ELSD ELSD. Cholesterol. Phosphatidylethanolamine. Phosphatidylcholine. Sphingomyelin. Lysophosphatidylcholine Column: DIOL, x. mm, µm Eluent A: IPA/Hexane/Water/ Ammonium Hydroxide 7.8//// Eluent B: Gradient: IPA/Hexane/Water/ Ammonium Hydroxide.8//8/. % B in 7 min, 8 min hold, % B in min, min hold Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = 8 ºC, gas flow =. SLM
UV ELSD Sample: Biodiesel Column: Cyano, x.6 mm, µm Eluent A:. % Acetic acid in Hexane Eluent B:. % Acetic acid in Methyl tert-butyl Ether Gradient: % A, hold min, 8 % B in min, hold min, 8 % B in min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = 9 ºC, gas flow =.8 SLM ELSD UV Diglycerides Triglycerides Monoglycerides Fatty acids Time (min) Sample: Sesame oil Column: C8, x. mm, µm Eluent A: Acetonitrile Eluent B: Dichloromethane Gradient: % B hold min, % B in min, 9 % B in min Flow rate: µl Detection: neb. = 6 ºC, evap. = ºC, gas flow =.6 SLM
DP ELSD DP DP DP DP 9 DP 88 DP 7 DP 6 DP DP DP Sample: Light corn syrup (Malto Oligosaccharides, Dp -Dp 9) Column: Hi-Plex Na, x 7.7 mm Eluent: Water Flow rate:. ml/min Injection volume: µl Temperature: 7 ºC Detection: neb. = ºC, evap. = 7 ºC, gas flow =. SLM Agilent ELSD RI. Sucrose. Glucose. Fructose. Sorbitol Sample: Apple and orange juice Column: Hi-Plex Ca, x 7.7 mm Eluent: Water Flow rate:.6 ml/min Injection volume: µl Apple Orange Temperature: 8 ºC Detection: neb. = ºC, evap. = ºC, gas flow =. SLM 6 6
ELSD. Citric acid. Tartaric acid. Malic acid. Glucose. Fructose 6. Succinic acid 7. Glycerol Sample: Column: Eluent: Flow rate: Australian Shiraz Hi-Plex H, x 7.7 mm. % TFA in Water. ml/min 6 7 Injection volume: µl Temperature: 8 ºC Detection: neb. = ºC, evap. = ºC, gas flow =. SLM. Maltitol. Ribitol. Iso-erythritol. Glycerol. Arabitol 6. Dulcitol 7. Sorbitol 6 7 Sample: Sugar alcohols Column: Hi-Plex Ca, x. mm Eluent: Water Flow rate:.6 ml/min Injection volume: µl Temperature: 8 ºC Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM 7
C ELSD. Thiamine. Pyridoxine HCI. Vitamin C. Nicotinic acid. Pyridoxine derivative 6. Niacinamide 7. Thiamine derivative 8. Riboflavin 6 Sample: Water Soluble Vitamins mix Column: C8, x.6 mm, µm Eluent A: Water Eluent B: Acetonitrile Gradient: % A in min, % B in min 7 8 Flow rate:.6 ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM 6 B B ELSD. Vitamin B. Biotin Sample: Nutritional supplements Column: PLRP-S, x.6 mm, µm Å Eluent A:. % TFA in water Eluent B:. % TFA in ACN Gradient: 99 % B in min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM 8
ELSD. Methyl paraben. Ethyl paraben. Propyl paraben. Butyl paraben. Heptyl paraben C Sample: Parabens Column: Agilent Pursuit C8, x. mm, µm Eluent A: Water Eluent B: Acetonitrile C Gradient: 7 % B in min, 7 9 % B in min Flow rate:. ml/min Injection volume: µl C ELS detection: neb. = ºC, as shown, gas flow =. SLM 9...6.8 Gas control on Agilent ELSD ( ) Gas control off Column: Agilent Pursuit C8, x. mm, µm Eluent A: Water Eluent B: Acetonitrile Gradient: 9 % B in min Flow rate:. ml/min Injection volume: µl (every min) Detection: neb. = ºC, evap. = ºC 6 8 9
UV ELSD Sample: PEG 6 Column: C8, x.6 mm, µm Eluent A: Water Eluent B: Acetonitrile Gradient: % B in min Flow rate:. ml/min Injection volume:. µl Detection: neb. = ºC, evap. as shown S/N - S/N - 6 S/N - 9. C (Gas. SLM) C (Gas. SLM) C (Gas. SLM). DMSO ELSD DMSO. Adenosine monophosphate. DMSO. Acetaminophen. Caffeine 6. Acetanilide 6. Phenacetin Column: C8, x.6 mm, µm Eluent A: Water Eluent B: Acetonitrile Gradient: % B in min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow as shown Time (min).
ELSD Agilent ELSD - 6. -aminopropanol. dexpanthenol Column: C8, x.6 mm, µm Eluent A:. % HFBA in Water Eluent B:. % HFBA in CAN Gradient: 9: A:B Flow rate:. ml/min Injection volume: µl mv Time (min) 6 8 Detection: neb. = ºC, evap. = 6 ºC, gas flow =. SLM UV ( ) ELSD. Streptomycin. Neomycin Column: C8, x.6 mm, µm Eluent A:. % Pentafluoropropionic acid (PFPA) in MeOH Eluent B:. % PFPA in 7 mm ammonium formate Gradient: : A:B Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = 8 ºC, gas flow =. SLM Time (min) 8 PEA ELSD.,6,9 Trioxaundecane. Phenylethylamine Column: C8, x. mm, µm Eluent A:. % TFA in Water Eluent B:. % TFA in Acetonitrile Gradient: -9 % in min Flow rate:. ml/min Injection volume: µl Detection: neb. = 6 ºC, evap. = ºC, gas flow =. SLM Time (min)
LC/MS ELSD ELSD LC/MS LC/MS LC/MS ELSD ELSD LC/MS. Haloperidol. Haloperidol metabolite I. Haloperidol metabolite II ( µg of each on column) Column: Agilent Pursuit XRs C8, x. mm, µm Eluent A:. % Formic acid in Water Eluent B:. % Formic acid in Acetonitrile Gradient: in 7 min, 8 in s, hold s, 8 in s Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = 8 ºC, gas flow =.9 SLM. 7. LC/MS Agilent ELSD. 7. Peak description MS detection (FS) S/N ELS detection S/N ELS detection sensitivity compared to MS detection. Haloperidol,8 7, 8x. Metabolite I,8 68, x. Metabolite II,6, x
C B UV UV UV Agilent ELSD UV Column: HILIC, x.6 mm, µm Eluent A: mm Ammonium acetate, ph 6 Eluent B: Acetonitrile Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =. SLM. Betaine Chinese lycium Betaine standard UV ELSD. Cimifugoside. Actein. 7-deoxyactein Column: C8, µ.6 mm, µm Eluent A:.% Formic acid in water Eluent B: Acetonitrile Gradient: % B in min, 6 % B in min, 6 % B in min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =. SLM UV detection: nm Agilent ELSD UV, nm Time (min) 6
. Hypericin. Hyperforin. Adhyperforin Agilent ELSD Column: C8, x.6 mm, µm Time (min) Eluent A:. % Ammonium formate, adjusted to ph. Eluent B: Acetonitrile Gradient: 9 % B in min, hold min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM. Daidzein. Genistein UV UV ELSD Column: C8, x.6 mm, µm Eluent A: Eluent B: 88// Water/MeOH/ % Acetic acid 98/ MeOH/ % Acetic Acid Time (min) Gradient: % B in min, % B in 8 min, 7 % in 6 min, 7 % in. min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM
Tween UV (RI) UV ELSD Sample: Tween at mg/ml Column: Eluent A: PLRP-S Å, x.6 mm, µm Water Time (min) 6 Eluent B: Acetonitrile Gradient: % B in min Flow rate:. ml/min Injection volume: µl Detection: neb. = ºC, evap. = ºC, gas flow =.6 SLM ( ) ELSD ELSD RI mv 7 Time (min) Cetrimide 6 8 Sample: Cetrimide Column: C8, x.6 mm, µm Eluent A:. % HFBA in Water Eluent B:. % HFBA in Acetonitrile Gradient: 9: A:B Flow rate:. ml/min Injectionvolume: µl Detection: neb. = ºC, evap. = 6 ºC, gas flow =. SLM
SFC/ELSD PEG (SFC) HPLC ELS SFC SFC/ELSD SFC (FID) Agilent ELSD ELSD Agilent ELSD SFC SFC/ELSD 6 7 6 counts counts counts PEG Time (min) PEG 6 Time (min) PEG Time (min) -77- www.agilent.com/chem/jp Agilent Technologies, Inc., Published in Japan, February, 99-9JAJP