J. Brew. Soc. Japan. Vol.78, No.6, p (1983) Identification of Asp. toxicarius and Some Other Aspergilli Taxonomic Studies on Japanese Industri

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1 J. Brew. Soc. Japan. Vol.78, No.6, p (1983) Identification of Asp. toxicarius and Some Other Aspergilli Taxonomic Studies on Japanese Industrial Strains of the Aspergilli (Part 35*) Hideya MURAKAMI, Masaaki INAIIASHI, and Toshio TANAKA Nippon Jozokyokai (Brewing Society of Japan) ** National Research Institute of Brewing (2-6 Takinogawa, Kita-ku, Tokyo 114) A strain NJK 4044 (=RMF 7133 of M. Christensen, IFO 31250) was a typical strain of Aspergillus toxicarius Murakami and produced aflatoxins remarkably by fluorescence test using APA-medium4). Two strains preserved for a long time under the name of A. candidus in a maker of tane-koji (mold starter) in Japan was proved to belong to A. oryzae brunneus. Among the 27 strains identified formerly with A. oryzae viridis about a half of them lost any green hue in their conidial heads when incubated at 30 for 25 days which were now placed in the other varieties, and a half of the remains kept this hue even after 100 days culture which showed characteristically broom-like to columnar types of conidial heads, and the results were shown in 102. Relations between the types and colors of conidial heads were shown in 104, and about 80% of the strains of broom-like to columnar types kept the green hue. The key character of the color of conidial heads, however, could not be replaced with that of the type of them from any view points. A type culture of A. oryzae viridis was assigned to a strain RIB 128 in 19711), which has also been called IFO 30113, ATCC and CBS and is preserved in Nippon Jozokyokai as NJK 128 since 1975 showing some metulae until now5,6). However, this strain was placed from now in A. oryzae oryzae because of its metulae, and then a lectotype, NJK 72, was determined as a correct type culture which was isolated by Murakami in 1953 from commercial tane-kojis for the brewing of sake and shoyu.

2 NJK 4044-RMF 7133 from Prof. Martha Chris- tensen, Univ. of Wyoming (1982), soil of Nebraska, Buffalo County, 55 miles s. of Miller ; a depth of 2. 0 meters in the exposed Peorian Loess of a roadcut, east-facing, U. S. A. (=IFO 31250)

村上稲橋田中;Asp.toxicariusほ 103 Mycological characteristics Abbreviations and signs : YG. yellowish BG, characters concerned. +-+++, the more remarkable In type of conidial heads ; A, globose.

4 村上稲橋田中;Asp.toxicariusほ 103 Mycological characteristics Abbreviations and signs : YG. yellowish BG, characters concerned , the more remarkable In type of conidial heads ; A, globose. B, radiate. vesicles ; A, globose. B, subglobose. C. spoon-like. Taxa: (a) oryzae. (b) か2 3菌 for the classification 株の同定 of some Aspergilli. brownish DG, dark W, white. -, lacking the characteristics corresponding with an increased number of +. B', loosely radiate. D, columnar. E, long columnar. In type of D, slim spoon. In type of conidia ; G, globose. 0, oval. viridis. (c) 104 brunneus. Relations (d) shown A.toxicarius, by number strains between the colors of conidial head in the having 103. no metulae in of the and types 27 strains s 102 and 分類する(viridis colummris)ことも考えられるが,以下のような理由もあって無理である (1) 型をkeyと Fig. 51 Crowded metulae of young cultures of A. toxicarius RMF 7133 of M. Christensen. (NJK 4044, IFO 31250) の分生子頭の型はすべて箒状(C) し球状(A) 放射状(B B')は D E型円柱状(D E)をなかった( 示 102) 分生子頭の色と型との関係について,102,103 からメトラをもつ株を除いた27株 104のようになる即ちC-D型は緑色味を保ち,A B型の約70%はの株の約80% 緑色味を失うので不安定な`緑色調'の代りに安定な`型'をkeyと 478 して and Fennell 13)のA. flavus 4818,NJK であるが30 2 3週 (この株は今回 1039)は明かに間で完全に緑色味を失う brunneusと同定された) (2)分生子頭の型と緑色味の関係について1966年上は84株の黄緑色株を見るとしたRaper columnaris(nrrl 村を調査したことがある14) この場合型を数値化することが困難なので便宜的に分生子頭の横径の大小によったのであるが,概してその小さい株は緑色味を保つ傾向を認めたものの,相関係数は-0.02で全く有意ではなかった (3)国税庁醸造試験所の糸状菌リスト均に醸協

5 1) H. Murakami : J.Gen. Appl. Microbiol., (1971) 11) H. Murakami, K. Hayashi and S. Ushijima : J. Gen. Appl. Microbiol., (1982) 12) Martha Christensen : Mycologia, Vol (1981) 13) K. B. Raper and D. I. Fennell : The Genus Aspergillus Williams & Wilkins Co., Baltimore, U. S. A. (1965),

Visual Evaluation of Polka-dot Patterns Yoojin LEE and Nobuko NARUSE * Granduate School of Bunka Women's University, and * Faculty of Fashion Science,

Visual Evaluation of Polka-dot Patterns Yoojin LEE and Nobuko NARUSE * Granduate School of Bunka Women's University, and * Faculty of Fashion Science, Bunka Women's University, Shibuya-ku, Tokyo 151-8523