Key words: enteropathogenic Escherichia coli, eaea, aggr, asta

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1 Key words: enteropathogenic Escherichia coli, eaea, aggr, asta

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3 Table 1 Nucleotide sequences of PCR primers and DNA probes

4 Table 2-1 O: H Serotypes and virulence-related genes of E. coli strains isolated from age-matched controls in Saga 1) OUT O untypable 2) HUT H untypable 3) HNM no motility

5 Table 2-2 O: H Serotypes and virulence-related genes of E. coli strains isolated from cases in Saga

6 Table 3-1 Prevalence of eaea-positive E. coli in cases and age-matched controls in Saga and Aichi Table 3-2 Prevalence of aggr-positive E. coil and asta-positive E. coli in cases and agematched controls in Saga Table 4 Prevalence of virulence-related genes of O-serotypable E. coil in cases and age-matched controls in Saga and Aichi Fisher' s exact test (All p values are two-tailed)

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8 crobio11998; 36: ) Yamazaki M, Saito M, Inuzuka K, Shima S, Tani- waki H, Itoh K: eaea genes in Escherichia coli derived from Japanese patients with sporadic diarrhea. JJ A Inf D 1997; 71: ) Sakazaki R, Tamura K, Prescott LM, Bencic Z: Bacteriological examination of diarrheal stools in Calcutta. Indian J Med Res 1971; 59: ) Ratchtrachenchai OA, Subpasu S, Ito K: Investi- 1) Cravioto A, Gross RJ, Scotland SM, Rowe B: An adhesive factor found in strain of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Curr Microbiol 1979; 3: ) Nataro JP, Kaper JB, Browne RR, Prado V, Vial P, Levine MM: Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells. Pediatr Infect Dis J 1987; 6: ) Nataro JP, Kaper JB: Diarrheagenic Escherichia coli. J Clin Microbiol Rev 1998; 11: ) Donnenberg MS, Tacket CO, James SP et al.: Role of the eaea gene in experimental enteropathogenic Escherichia coli infection. J Clin Invest 1993; 92: ) Biswas R, Nelson EAS, Lewindon PJ, Lyon DJ, Sullivan PB, Echeverria P: Molecular epidemiology of Escherichia coli diarrhea in children in Hong Kong. J Clin Microbiol 1996; 34: ) Forestier C, Meyer M, Favre-Bonte S et al.: Enteroadherent Escherichia coli and diarrhea in children: a prospective case-control study. J Clin Microbiol 1996; 34: ) Nataro JP, Yikang D, Cookson S et al.: Heterogeneity of enteroaggregative Escherichia coli virulence demonstrated in volunteers. J Infect Dis 1995; 171: ) Gomes TAT, Vieira MAM, Abe CM, Rodrigues D, Griffin PM, Ramos SRTS: Adherence patterns and adherence-related DNA sequences in Escherichia coli isolates from children with and without diarrhea in Sao Paulo City, Brazil. J Clin Mi- gation on enteroaggregative Escherichia con infection by multiplex PCR. Bull Dept Med Sci 1997; 39: , 16) Gonzalez R, Diaz C, Mrino M, Cloralt R, Pequeneze M, Perez-Schael I: Age-specific prevalence of Escherichia coli with localized and aggregative adherence in Venezuelan infants with acute diarrhea. J Clin Microbiol 1997; 35: ) Adu-boie J, Frankel G, Bain C et al.: Detection of intimins ƒ, ƒà, ƒá and ƒâ, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbio11998; 36: ) Fernando NG, Eslava C, Villaseca JM et al.: In vitro effects of a high-molecular-weight heat-labile enterotoxin from enteroaggregative Escherichia coli. Infect Immun 1998; 66: ) Itoh Y, Nagano I, Kunishima M, Ezaki T: Laboratory investigation of enteroaggregative Escherichia coli Ountypeable: H 10 associated with a massive outbreak of gastrointestinal illness. J Clin Microbio11997; 35: ) Koopmans MP, Goosen ES, Lima AA et al.: Association of torovirus with acute and persistent diarrhea in children. Pediatr Infect Dis J 1997; 16:

9 The Prevalence of Virulence-related Genes, eaea, aggr and asta, of Localized and Aggregative-adherent Escherichia coli (EPEC and EAggEC) in Healthy Children and Age-matched Patients with Diarrhea Kazuo MORIYA1), Noriko SUMI1) Saga Prefectural Institute of Public Health Aichi Prefectural Institute of Public Health National Institute of Public Health The prevalence of virulence-related genes of localized-and aggregated-adherent Escherichia coli (EPEC and EAggEC), such as eaea, aggr and asta was compared between E. coli isolated from 0 to 5 year old children with and without diarrhea in Saga Prefecture. In the case of eaea, 233 cases in Aichi Prefecture were included. The subjects were 74 diarrheal patients from which no diarrheagenic bacteria were detected besides E. coli. The control subjects were 304 nursery school children without diarrhea, and E. coli was isolated from 278 children in which 105 strains were of 0-serotype. EaeApositive E. coli was isolated from nine (12.2%) Saga cases, 19 (8.2%) Aichi cases and 6 (5.7%) control subjects aggr -positive E. coli was isolated from 10 (13.5%) cases and 6 (5.7%) control subjects and asta -positive E. coli from 10 (13.5%) cases and 14 (13.3%) control subjects. No significant difference (p>0.05) was observed in the prevalence of eaea, aggr and asta between healthy and diarrheal children, even in age-matched and 0-serotypable E. coli limited comparisons. The pathogenicity of EPEC and EAggEC should be investigated, considering other known or unidentified factors.

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