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6 sensitivity to Kathon(R) CG, Contact Dermatitis, 14: , Tosti, A., Manuzzi, P. and Padova, M.P.: Contact dermatitis to Kathon CG, Contact Dermatitis, 14: , Cardin, C.W., Weaver, J.E. and Bailey, P.: Dose-response assesments of Kathon(R) biocide. (II). Threshold prophetic patch testing, Contact Dermatitis, 15: 10-16, Gruverger, B., Persson, K., Bjorkner, B., 1. DeGroot, A.C., Lien, D.H. and Weyland, J.W. : Kathon(R) CG: Cosmetic allergy and patch test sensitization, Contact Dermatitis, 12: 76-80, DeGroot, A.C., Lien, D.H., Nater, J.P. and van Ketel, W.G.: materials and preservatives, 12: 87-92, Patch tests with fragrance Contact Dermatitis, 3. Weaver, J.E., Cardin, C.W. and Maibach, H. I. : Dose-response assessments of Kathon(R) biocide. (I). Diagnostic use and diagnostic threshold patch testing with sensitized humans, Contact Dermatitis, 12: , Maibach, H.I.: Diagnostic patch test concentration for Kathon CG, Contact Dermatitis, 13: , Bjorkner, B., Bruze, M., Dahlquist, I.,Fregert, S., Gruverger, B. and Persson, K.: Contact allergy to the preservative Dermatitis, 14: 85-90, Kathon(R) CG, Contact 6. Hjorth, N. and Roed-Petersen, J.: Patch test Bruze, M., Dahlquist, I. and Fregert, S.: Demonstration of Kathon(R) CG in some commercial products, Contact Dermatitis., 15: 24-27, Hannuksela, M.: Rapid increase in contact allergy to Kathon(R) CG in Finland, Contact Dermatitis, 15: , {ŠL À Y: Ú G ç wž ƃpƒbƒ`ƒeƒXƒg, ç w,19: , ƒ [ƒ EƒAƒ ƒh Eƒ} [ƒx EƒWƒƒƒpƒ Š Ž ïžðž. 13. Chan, P.K., Baldivin, R.C., Rarsons, R.D., Moss, J.N., Stiratelli, R., Smith, J.M. and Hayes, A.W.: Kathon biocide: manifestation of delayed contact dermatitis in guinea pigs is dependent on the concenration for induction and challenge, J.Invest. Dermatol., 81: , DeGroot, A.C., Bos, J.D., Jagtman, B.A., Bruynzeel, D.P., van Joost, T. and Weyland, J.W.: Contact allergy to preservatives- II, Contact Dermatitis, 15: , Lewis, P.G.: Contact allergy to preservative Kathon(R) CG (Letter to the editor), Contact Dermatitis, 14: , 1986.

7 Allergic Cosmetic Dermatitis from Kathon CG Kayoko Watanabe, Tetsuro Sugai and Fukiko Okuno Department of Dermatology, Osaka Kaisei Hospital (Chief : Dr. T. Sugai) Osaka, Japan 531 Key words : Kathon CG-biocide-isothiazoline-allergic contact dermatitis-hplc Kathon is one of the best preservatives, and Kathon WT is widely used in cooling tower water, papermill process water and air washers. Kathon CG was said to be used extensively in cosmetics and toiletries for 10 years in Europe and 5 years in the USA, though it has not been permitted to use in cosmetics and toiletries in Japan. Safety evaluation of Kathon CG was asked through the Kao Company by the Rohm and Haas, USA. Thus, a prophetic patch test was carried out in 149 patients with contact dermatitis. The test material supplied was an aqueous solution of Kathon CG of 100 ppm a. i. Finn-chamber and Scanpor-tape were used as units. Surprisingly, allergic reactions to Kathon CG were found in 3 female patients who used the same brand of European cosmetics ppm of 5-chloro-2-mehtyl-4-isothiazoline-3-one was detected with HPLC and GC/MS in a moisturizing cream which one of the patients brought out. The analytical reference standard of Kathon CG was found to contain the following1. 15% of the monochloro-compound, 0. 35% of 2-methyl-4-isothiazoline-3-one, 23. 0% of magnesium-salts and 75. 5% of water. Therefore, it was estimated that a total active component was ppm. In a patient out of the three, allergic reactions were seen with 4 products out of six which she brought out. It will be reasonable, thus, to consider that the use of Kathon CG should be limited to the rinse-off type cosmetics in a very low concentration.

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