2010年度 タカラバイオ ディスカウントキャンペーン

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2 p.2 p.3 5 p.6 9 p p.19 p.25 p p p.31 p.31 p p p p.24 1

3 40%OFF (RR037A+RR420A) (RR037A+RR820A) (RR037A+RR091A) (RR036A+RR420A) (RR036A+RR820A) (RR036A+RR091A) (RR047A+RR420A) (RR047A+RR820A) (RR047A+RR091A) 2

4 SYBR Premix Ex Taq (Tli RNaseH Plus) SYBR Premix Ex Taq II (Tli RNaseH Plus) PrimeScript RT reagent Kit with gdna Eraser (Perfect Real Time) 3

5 MightyAmp DNA Polymerase Ver.2 MightyAmp for Card MightyAmp for FFPE TaKaRa DEXPAT Easy In-Fusion HD Cloning Kit 4

6 plvsin-cmv plvsin-ef1 Tough Decoy mirna-blocking Expression Vector Synthetic sirna Quantitation Core Kit 5

7 Taq TaKaRa Ex Taq TaKaRa LA Taq TaKaRa LA Taq with GC Buffer SpeedSTAR HS DNA Polymerase TaKaRa Taq PrimeSTAR PrimeSTAR HS DNA Polymerase PrimeSTAR GXL DNA Polymerase PrimeSTAR Max DNA Polymerase EmeraldAmp PCR Master Mix SapphireAmp Fast PCR Master Mix MightyAmp DNA Polymerase Ver.2 MightyAmp for Card MightyAmp for FFPE NucleoSpin Extract II 6

8 TaKaRa Ex Taq TaKaRa Ex Taq Hot Start Version TaKaRa LA Taq TaKaRa LA Taq with GC Buffer PrimeSTAR HS DNA Polymerase TaKaRa Ex Taq TaKaRa Ex Taq (Mg 2+ free Buffer) TaKaRa Ex Taq Hot Start Version TaKaRa LA Taq TaKaRa LA Taq with GC Buffer TaKaRa LA Taq Hot Start Version TaKaRa Taq TM TaKaRa Taq TM Mg 2 free Buffer TaKaRa Taq TM Hot Start Version SpeedSTAR HS DNA Polymerase TaKaRa Z-Taq Pyrobest DNA Polymerase PrimeSTAR GXL DNA Polymerase PrimeSTAR Max DNA Polymerase PrimeSTAR HS DNA Polymerase PrimeSTAR HS (Premix) PrimeSTAR HS DNA Polymerase with GC Buffer 7

9 EmeraldAmp PCR Master Mix SapphireAmp Fast PCR Master Mix MightyAmp DNA Polymerase Ver.2 MightyAmp for Card MightyAmp for FFPE 20%OFF NucleoSpin Extract II 8

10 TaKaRa PCR Thermal Cycler Dice Gradient TaKaRa PCR Thermal Cycler Dice Standard 650, ,000 TaKaRa PCR Thermal Cycler Dice mini 400,000 TaKaRa PCR Thermal Cycler Dice Gradient 650, ,000 TaKaRa PCR Thermal Cycler Dice Standard 550, ,000 TaKaRa PCR Thermal Cycler Dice mini 400, ,000 9

11 Step-1 NucleoSpin RNA II NucleoSpin RNA XS Step-2 PrimeScript RT Step-3 Perfect Real Time Step-4 SYBR Premix Ex Taq (Tli RNaseH Plus) SYBR Premix Ex Taq II (Tli RNaseH Plus) SYBR Premix DimerEraser (Perfect Real Time) SYBR Premix Ex Taq GC (Perfect Real Time) Thermal Cycler Dice Real Time System II 10

12 NucleoSpin RNA II NucleoSpin RNA XS 11

13 CellAmp Direct RNA Prep Kit for RT-PCR Real Time CellAmp Direct Prep Kit for RT-PCR Real Time & Protein Analysis CellAmp Whole Transcriptome Amplification Kit Real Time Ver.2 SimplePrep TM reagent for DNA 12

14 PrimeScript RT reagent Kit (Perfect Real Time) PrimeScript RT Master Mix (Perfect Real Time) PrimeScript RT reagent Kit with gdna Eraser (Perfect Real Time) H32 M37 Rsp18 H14 H

15 SYBR Premix Ex Taq TM II (Tli RNaseH Plus) SYBR Premix Ex Taq TM (Tli RNaseH Plus) SYBR Premix DimerEraser (Perfect Real Time) SYBR Premix Ex Taq TM GC (Perfect Real Time) 14

16 SYBR qrt PCR Set 1 (RR037A+RR420A) SYBR qrt PCR Set 2(RR037A+RR820A) SYBR qrt PCR Set 3(RR037A+RR091A) SYBR qrt PCR Set 4(RR036A+RR420A) SYBR qrt PCR Set 5(RR036A+RR820A) SYBR qrt PCR Set 6(RR036A+RR091A) SYBR qrt PCR Set 7(RR047A+RR420A) SYBR qrt PCR Set 8(RR047A+RR820A) SYBR qrt PCR Set 9(RR047A+RR091A) 40%OFF SYBR Premix Ex Taq II (Tli RNaseH Plus) SYBR Premix DimerEraser (Perfect Real Time) SYBR Premix Ex Taq (Tli RNaseH Plus) SYBR Premix Ex Taq GC (Perfect Real Time) SYBR Premix Ex Taq TM II (Perfect Real Time) SYBR Premix Ex Taq TM (Perfect Real Time) SYBR Premix Ex Taq TM II 5 ml, ROX plus SYBR Premix Ex Taq TM II 5 ml SYBR Premix Ex Taq TM 5 ml, ROX plus SYBR Premix Ex Taq TM 5 ml 15

17 One Step SYBR PrimeScript PLUS RT-PCR Kit Perfect Real Time One Step SYBR PrimeScript RT-PCR Kit Perfect Real Time One Step SYBR PrimeScript RT-PCR Kit II Perfect Real Time 16

18 PrimeScript RT reagent Kit (Perfect Real Time) PrimeScript RT Master Mix (Perfect Real Time) PrimeScript RT reagent Kit with gdna Eraser (Perfect Real Time) Premix Ex Taq TM Perfect Real Time PrimeScript RT-PCR Kit Perfect Real Time One Step PrimeScript RT-PCR Kit Perfect Real Time 17

19 Thermal Cycler Dice Real Time System Thermal Cycler Dice Real Time System II 3,750,000 3,100,000 Thermal Cycler Dice Real Time System II MRQ 3,900,000 3,200,000 Thermal Cycler Dice Real Time System Single 2,850,000 1,980,000 Thermal Cycler Dice Real Time System Single MRQ 3,000,000 2,080,000 96well Hi-Plate for Real Time Sealing Film for Real Time 0.2 ml Hi-8-Tube ml Hi-8-Flat Cap ml 8-strip tube, individual Flat Caps 8 18

20 NucleoSpin RNA II NucleoSpin RNA XS Fruit-mate for RNA Purification Yeast Processing Reagent (for total RNA preparation) High-Salt Solution for Precipitation (Plant) Gen Oligotex -dt30<super> mrna Purification Kit (From Total RNA) Gen High Recovery TaKaRa RECOCHIP TaKaRa DEXPAT TaKaRa DEXPAT Easy NucleoSpin Plasmid QuickPure 19

21 PrimeScript RTase PrimeScript II 1st strand cdna Synthesis Kit TaKaRa Ex Taq PrimeSTAR HS DNA Polymerase PrimeSTAR Max DNA Polymerase PrimeSTAR GXL DNA Polymerase PrimeScript One Step RT-PCR Kit Ver.2 PrimeScript High Fidelity RT-PCR Kit DNA Ligation Kit Mighty Mix DNA Ligation Kit LONG In-Fusion HD Cloning Kit Mighty TA-cloning Reagent Set for PrimeSTAR Mighty Cloning Reagent Set Blunt End T-Vectors E. coli HST08 Premium Competent Cells E. coli JM109 Competent cells E. coli HST08 Premium Electro-Cells NuSieve GTG Agarose NuSieve 3:1 Agarose 100 bp DNA Ladder 100 bp DNA Ladder Dye Plus 1 kb DNA Ladder 20

22 T4 DNA Ligase T4 Polynucleotide Kinase T4 DNA Polymerase T4 RNA Ligase Alkaline Phosphatase E. coli C75 Alkaline Phosphatase Calf intestine Ribonuclease H RNase H TaKaRa DNA Ligation Kit LONG DNA Ligation Kit Mighty Mix DNA Ligation Kit Ver.2.1 DNA Ligation Kit Ver.1 DNA Blunting Kit Mighty Cloning Reagent Set (Blunt End) Mighty TA-cloning Kit Mighty TA-cloning Reagent Set for PrimeSTAR T-Vector pmd20 T-Vector pmd19 Simple E. coli HST08 Premium Competent Cells E. coli HST04 dam /dcm Competent Cells E. coli JM109 Competent Cells E. coli DH5 Competent Cells E. coli HB101 Competent Cells E. coli HST08 Premium Electro-Cells E. coli JM109 Electro-Cells E. coli DH5 Electro-Cells E. coli HB101 Electro-Cells 21

23 PrimeScript 1st strand cdna Synthesis Kit PrimeScript II 1st strand cdna Synthesis Kit PrimeScript Reverse Transcriptase Random Primer (hexadeoxyribonucleotide mixture; pd (N) 6 ) Random Primer (nonadeoxyribonucleotide mixture; pd (N) 9 ) Oligo(dT) 15 Primer PrimeScript High Fidelity RT-PCR Kit PrimeScript II High Fidelity RT-PCR Kit PrimeScript II High Fidelity One Step RT-PCR Kit PrimeScript RT-PCR Kit PrimeScript One Step RT-PCR Kit Ver.2 PrimeScript One Step RT-PCR Kit Ver.2 (Dye Plus) PrimeScript PrimeScript 1st strand cdna Synthesis Kit 22

24 In-Fusion HD Cloning Kit w/cloning Enhancer In-Fusion HD Cloning Kit w/nucleospin In-Fusion HD Cloning Kit 5 5 5In-Fusion HD Enzyme Premix & dh2o 23

25 SMARTer RACE cdna Amplification Kit 20%OFF SMARTer PCR cdna Synthesis Kit SMARTer Pico PCR cdna Synthesis Kit In-Fusion SMARTer Directional cdna Library Construction Kit SMART cdna Library Construction Kit SMART mrna Amplification Kit 24

26 Matchmaker Gold Yeast Two-Hybrid System Matchmaker Gold Yeast One-Hybrid Library Screening System Make Your Own Mate & Plate Library System Two-Hybrid Yeast Media Set 2 Yeast Media Set 2 Plus One-Hybrid Yeast Media Set 1 Yeast Media Set 1 Plus 20%OFF YPDA Broth SD/-Leu/-Trp Broth YPDA with Agar SD/-Leu/-Trp with Agar SD/-Trp Broth SD/-His/-Leu/-Trp Broth SD/-Trp with Agar SD/-His/-Leu/-Trp with Agar SD/-Leu Broth SD/-Leu/-Trp/-Ura Broth SD/-Leu with Agar SD/-Leu/-Trp/-Ura with Agar SD/-His Broth SD/-Ade/-His/-Leu/-Trp Broth SD/-His with Agar SD/-Ade/-His/-Leu/-Trp with Agar SD/-Ura Broth SD/-His/-Leu/-Trp/-Ura Broth SD/-Ura with Agar SD/-His/-Leu/-Trp/-Ura with Agar 25

27 Aat II Ban II HgiJ II Acc I BciT130 I EcoR II, Mva I Acc II FnuD II Bcn I Cau II Acc III BspM II Bgl I Afa I Rsa I Bgl II Afl II Bgl II Alu I Bln I Avr II Aor13H I BspM II, Acc III BmeT110 I Ava I Aor51H I Eco47 III BmgT120 I Cfr13 I, Asu I Apa I Bpu1102 I Esp I Apa I Bsp1286 I Sdu I ApaL I Bsp1407 I Bal I BspT104 I Asu II, Nsp V BamH I BspT107 I HgiC I BamH I BssH II BseP I 26

28 Bst1107 I Sna I Bst P I BstE II, EcoO65 I Bst X I Cfr10 I Cla I Cla I Cpo I Rsr II Dpn I Dra I Aha III Dra I Aha III Eae I Cfr I Eam1105 I Eco52 I Xma III Eco81 I Sau I EcoO65 I Bst E II, Bst P I EcoO109 I Dra II EcoR I EcoR I EcoR V EcoR V EcoT14 I Sty I EcoT22 I Ava III Fba I Bcl I Fok I Hae II Hae III Hae III Hap II Hpa II, Msp I Hap II Hpa II, Msp I Hha I Hin1 I Acy I, Bbi II Hinc II Hind II Hinc II Hind II Hind III Hind III Hinf I Hinf I Hpa I Kpn I Kpn I Mbo I Sau3A I Mbo II Mf l I Xho II Mlu I Mlu I Msp I Hap II, Hpa II Msp I Hap II, Hpa II Mun I Mfe I Nae I Nco I Nco I Nde I Nhe I Not I Not I 27

29 Nru I Nsb I Avi II, Mst I PmaC I PshA I PshB I Vsp I Psp1406 I Acl I Pst I Pst I Pvu I Pvu II Pvu II Sac I Sac I Sac II Sal I Sal I Sau3A I Mbo I Sau3A I Mbo I Sca I Sfi I Sma I Sma I Smi I Swa I SnaB I Spe I Sph I Sse8387 I Sse8387 I Ssp I Stu I Taq I TthHB8 I Tth111 I Van91 I PflM I VpaK11B I Ava II Xba I Xba I Xho I Xho I Xsp I Bfa I, Mae I McrBC mrna Interferase -MazF 28

30 20 bp DNA Ladder Dye Plus 50 bp DNA Ladder Dye Plus 100 bp DNA Ladder Dye Plus 200 bp DNA Ladder Dye Plus 250 bp DNA Ladder Dye Plus 500 bp DNA Ladder Dye Plus 1 kb DNA Ladder Dye Plus Wide-Range DNA Ladder 50-10,000 bp Wide-Range DNA Ladder 100-2,000 bp Wide-Range DNA Ladder 100-5,000 bp 20 bp DNA Ladder 100 bp DNA Ladder 200 bp DNA Ladder 500 bp DNA Ladder 1 kb DNA Ladder 2.5 kb DNA Ladder -EcoT14 I digest -EcoT14 I/Bgl II digest -Bst P I digest -Hind III digest phy Marker X174 Hae III digest X174 Hinc II digest 29

31 20 bp 50 bp bp 1,500 1, bp 100 bp 200 bp 250 bp 500 bp 1 kb 2.5 kb 1,500 1, bp 4,000 3,000 2,500 2,000 1, bp 4,500 3,000 2,250 1,500 1, bp 5,000 4,000 3,000 2,000 1,500 1, bp kb kb Wide-Range 100-2,000 bp100-5,000 Wide-Range bp 2,000 1, bp 5,000 3,000 2,000 1,500 1, bp -EcoT14 I -Hind Ⅲ phy X174 Hae Ⅲ X174 Hinc Ⅱ 19,329 7,743 6,223 4,254 3,472 2,690 1,882 1, ,130 9,416 6,557 4,361 2,322 2, ,870 2,016 1,360 1, ,353 1, bp bp bp bp bp kda , Low High kda Broad kda NuSieve GTG Agarose NuSieve 3:1 Agarose SeaKem LE Agarose SeaKem Gold Agarose MetaPhor Agarose 20%OFF PBS Phosphate Buffered Salts Tablets TBE Tris-borate-EDTA powder Protein Molecular Weight Marker Low Protein Molecular Weight Marker High Protein Molecular Weight Marker Broad 30

32 ⑪レンチウイルス関連 20~ plvsin-cmv/ef1α Vector パッケージングシステムほか 製 品 名 概 要 plvsin-cmv Neo Vector plvsin-cmv Hyg Vector plvsin-cmv Pur Vector plvsin-ef1α Neo Vector plvsin-ef1α Hyg Vector plvsin-ef1α Pur Vector Lenti-X HTX Packaging System Lenti-X HTX Ecotropic Packaging System Lenti-X qrt-pcr Titration Kit 容量 S I N 型レンチウイルスベクター CMVプロモーター搭載 SIN型レンチウイルスベクター EF1αプロモーター搭載 レンチウイルスパッケージングシステム VSV-G レンチウイルスパッケージングシステム Ecotropic 最短30秒で力価を簡易判定 レンチウイルス濃縮試薬 超遠心不要で 100倍に濃縮 Lenti-X Concentrator 20 µg 通常価格 キャンペーン価格 ,000 39, ,000 39, ,000 39, ,000 39, µg ,000 39,200 56,000 39,200 20回 , ,400 ラ 営 60回 , ,600 ラ 営 20回 , ,400 ラ 営 200回 ,000 76,000 20回 ,000 25,600 50回 ,000 55, 回 , , ml ,000 24, ml ,000 88, 力価測定キット qrt-pcr Lenti-X GoStix 製品コード 従来のLenti-X HT Packaging System 製品コード のLentiphos HTに替えて Xfect Transfection Reagent が含まれており より使いやすくなりました なお 従来品は在庫限りで販売を終了いたします 従来品もキャンペーン対象です ラ 営 ご購入に際してライセンス確認書が必要となります 営利施設の場合 ご購入前にライセンス 有償 を取得する必要があります plvsin-cmv/ef1αベクター 5 LTR ClontechのLenti-X HTXパッケージングシステムとの 組合せで高力価レンチウイルスを産生 調製した組換えレンチウイルスはほぼ全ての哺乳類細胞に 遺伝子導入が可能 3 LTR/ΔU3のSIN型ベクターで安全性が高い PBS ψ Ampr RRE puc ori plvsin-cmv cppt/ct S plvsin-ef1α IE or α 目的遺伝子を挿入 MCS 3 LTR/ΔU3 WPRE (or or ) tta Xfectによる トランスフェクション 実験例 ZsGreen1蛍光タンパク質遺伝子を 搭載したpLVSIN-CMV Pur Vector とLenti-X パッケージングシステム を用いて組換えレンチウイルス を 調製した レンチウイルス300 μlを用 いて ポリブレン法によりHT1080細 胞へ遺伝子導入を行った3日後の蛍光 顕微鏡写真を示す 力価 6X10⁵ ivp/ml Lenti-X HTX Packaging Mix Lenti-X 293T Cell Line ⑫ 抗体関連 容 量 製 品 名 Human IgG EIA Kit 製品コード 通常価格 キャンペーン価格 MK ,000 70,700 Mouse IgG EIA Kit 96回 2 MK ,000 70,700 Rat IgG EIA Kit 96回 2 MK ,000 70,700 96回 Anti-Human Oct4, Monoclonal 0.1 mg M221 60,000 42,000 Anti-Human Lin28, Monoclonal 0.1 mg M222 60,000 42,000 Anti-Human Sox2, Monoclonal 0.1 mg M223 60,000 42,000 表示価格はすべて税別です タカラバイオ ディスカウントキャンペーン 期間 2010年12月1日 水 2011年2月28日 月 ご注文分まで

33 microrna Archives Tough Decoy mirna-blocking Expression Vector 20%off small RNA Gel Extraction Kit NucleoSpin mirna 20%off small RNA Cloning Kit 20%off Mir-X mirna qrt-pcr SYBR Kit 20%off 20%OFF small RNA Gel Extraction Kit NucleoSpin mirna ssrna Ladder Marker sirna Ladder Marker small RNA Cloning Kit 20%OFF Mir-X mirna qrt-pcr SYBR Kit 32

34 20%OFF Tough Decoy mirna-blocking Expression Vector (pbasi-neo) Tough Decoy mirna-blocking Expression Vector (pbasi-pur) Tough Decoy mirna-blocking Expression Vector (psinsi-neo) 20%OFF Synthetic sirna Quantitation Core Kit 33

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