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- ゆきさ さわなか
- 7 years ago
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Transcription
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13 ,939 (26 79) 10,939 5,267 5,672 3,756 7,183 1) 108 2,896 2) 152 3,292 3) ) ) NPO 7 153
14 Web Le Message
15 () 14:00 15:30 E A E COD 2 3 C C
16 () 13:3016:50 21 () () () () PM2.5 () 1,3-[(2,3)]() ( 1 ) 41 ( 2 ) 41 12
17 H RKB H H KBCJ:COM 1) 7 H H27. 7 SFTS 9 H27. 9!? 10 H27.12,! 11 H28. 3 PM2.5 2)() ()
18 1 3
19 1 3 E
20 1 3
21 1 3
22 1 3
23 LAS4-t- 2, , , (LAS)4-t- 2, ,229 1
24 1 3 4 p H 68 PCB , , ,2-62 1, , ,1, ,1, TOC , , ,229 2 No. 1 No.1 2 No.2 3 No No.1 7 No No.1 11 No.2 12 No.3 13 No No.1 17 No No.1 20 No
25 t- A 4-t- A BOD ph , ) 4 16 () PM , ,398 1 ph 2 ph
26 1 3 ph ,2-1, PM 2.5 PM
27 () () , , , , , ,003 PCB SS
28
29 ,263 1, ,833 1, ,2633,
30
31 HIV HIV 3,172 3,172 1,539 1, ,890 5, HIVHIV-1HIV , HIV ,664 3,353 3,722 3,461 3, , ,539 IgA IgG 134 1, (9%) (88%) (4%) (10%) 1, (87%) (3%) ,550 1,468 1,677 1,633 1,539 IgA IgA 11% 11% 9% 9% 9% IgG IgG 18% 14% 13% 13% 10% ,867 1, EIA EIA
32 ,243 6, , ,100 8,270 2, TPHA RPR ,
33 , , , , , , , ,467 ( ) 4
34 PCB ' 4,6321,200' 58 36' 13,9208,640' 24 1' 1, ' 9 0' 8 0' 408 0' ' 20,697'9,912' ' 0' 560' 0' 9' 1' 719' 79' 10' 6' 820' 492' 12' 0' 960' 0' 8' 4' 89' 44' 46' 11' 3,148' 615' ppm 3 PCB 0.01ppm
35 1 3 3 P C B (168) (11505) (78) (58) (233) (12) () () (531) () (9912) (615) (1) () () () () (56) () (7) (2) () () () () () () () () () () () () () () () () () () () () () () () (7) (495) () () () (3) () () () () () (492) () () () () () () () () () () (6) (47) () () () (1) () () () () () (44) () () () () () () () () (2) () (1) (152) () () () () () () () () (72) (79) (1) () () () () () () () () () (1) (3) (3) () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () (8) (1232) (6) (4) (1) () () () (21) () (1200) () () () () () () () () () () () (54) (8746) (13) (8) (1) (8) () () (69) () (8640) () () () () () () () () (7) () () (40) (500) (12) (8) (162) () () () (318) () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () (11) (43) (14) (6) (11) () () () (12) () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () (28) (231) (30) (32) (58) () () () (111) () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () () (8) (44) () () () () () () () () () () () () () () () (44) () () () () (4) (12) () () () () () () () () () () () () () () () (12) () () () () () () () () () () () () () () () () () () () () () () () () () ()
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41 /2 No. (1) 0.01 No. (61) 0.01 (62) 0.01 (2) 0.01 (63) 0.01 (3) 0.01 (64) 0.01 (4) 0.01 (65) 0.01 (5) 0.01 (66) 0.01 (6) 0.01 (67) 0.01 (7) 0.01 (68) 0.01 (8) 0.01 (69) 0.01 (9) 0.01 (70) 0.01 (10) 0.01 (71) 0.01 (11) 0.01 (72) 0.01 (12) 0.01 (73) 0.01 (13) 0.01 (74) 0.01 (14) 0.01 (75) 0.01 (15) 0.01 (76) 0.01 (16) 0.01 (77) 0.01 (17) 0.01 (78) 0.01 (18) 0.01 (79) 0.01 (19) 0.01 (80) 0.01 (20) 0.01 (81) 0.01 (21) (82) 0.01 (22) 0.01 (83) 0.01 (23) 0.01 (84) 0.01 (24) 0.01 (85) 0.01 (25) 0.01 (86) 0.01 (26) 0.01 (87) 0.01 (27) 0.01 (88) 0.01 (28) 0.01 (89) 0.01 (29) 0.01 (90) 0.01 (30) 0.01 (91) 0.01 (31) 0.01 (92) 0.01 (32) 0.01 (93) 0.01 (33) 0.01 (94) 0.01 (34) 0.01 (95) 0.01 (35) 0.01 (96) 0.01 (36) 0.01 (97) 0.01 (37) 0.01 (98) 0.01 (38) 0.01 (99) 0.01 (39) 0.01 (100) 0.01 (40) 0.01 (101) 0.01 (41) 0.01 (102) 0.01 (42) 0.01 (103) 0.01 (43) 0.01 (104) 0.01 (44) 0.01 (105) 0.01 (45) 0.01 (106) 0.01 (46) 0.01 (107) 0.01 (47) 0.01 (108) 0.01 (48) 0.01 (109) 0.01 (49) 0.01 (110) 0.01 (50) 0.01 (111) 0.01 (51) 0.01 (112) 0.01 (52) 0.01 (113) 0.01 (53) 0.01 (114) 0.01 (54) 0.01 (115) 0.01 (55) 0.01 (116) (56) 0.01 (117) 0.01 (57) 0.01 (118) 0.01 (58) 0.01 (119) 0.01 (59) 0.01 (120) 0.01 (60) 0.01 (121) ppm
42 /2 No. No. (122) 0.01 (183) 0.01 (123) 0.01 (184) 0.01 (124) 0.05 (185) 0.01 (125) 0.01 (186) 0.01 (126) 0.01 (187) 0.01 (127) 0.01 (188) 0.01 (128) 0.01 (189) 0.01 (129) 0.01 (190) 0.01 (130) 0.01 (191) 0.01 (131) 0.01 (192) 0.01 (132) 0.01 (193) 0.01 (133) 0.01 (194) 0.01 (134) 0.01 (195) 0.01 (135) 0.01 (196) 0.01 (136) 0.01 (197) 0.01 (137) 0.01 (198) 0.01 (138) 0.01 (199) 0.01 (139) 0.01 (200) 0.01 (140) 0.01 (201) 0.01 (141) 0.01 (202) 0.01 (142) 0.01 (203) 0.01 (143) 0.01 (204) 0.01 (144) 0.01 (205) 0.01 (145) 0.01 (206) 0.01 (146) 0.01 (207) 0.01 (147) 0.01 (208) 0.01 (148) 0.01 (209) 0.01 (149) 0.01 (210) 0.01 (150) 0.01 (211) 0.01 (151) 0.01 (212) 0.01 (152) 0.01 (213) 0.01 (153) 0.01 (214) 0.01 (154) 0.01 (215) 0.01 (155) 0.01 (216) 0.01 (156) 0.01 (217) 0.01 (157) 0.01 (218) 0.01 (158) 0.01 (219) 0.01 (159) 0.01 (220) 0.01 (160) 0.01 (221) 0.01 (161) 0.01 (222) 0.01 (162) 0.01 (223) 0.01 (163) 0.01 (224) 0.01 (164) 0.01 (225) 0.01 (165) 0.01 (226) 0.01 (166) 0.01 (227) 0.01 (167) 0.01 (228) 0.01 (168) 0.01 (229) 0.01 (169) 0.01 (230) 0.01 (170) 0.01 (231) 0.01 (171) 0.01 (232) 0.01 (172) 0.01 (233) 0.01 (173) 0.01 (234) 0.01 (174) 0.01 (235) 0.01 (175) 0.01 (236) 0.01 (176) 0.01 (237) 0.01 (177) 0.01 (238) 0.01 (178) 0.01 (239) 0.01 (179) 0.01 (240) 0.01 (180) 0.01 (181) 0.01 (182) ppm
43 No. No. (1) 0.01 (37) 0.01 (2) 0.01 (38) 0.01 (3) 0.01 (39) 0.01 (4) 0.01 (40) 0.01 (5) 0.01 (41) 0.01 (6) 0.01 (42) 0.01 (7) 0.01 (43) 0.01 (8) 0.01 (44) 0.01 (9) 0.01 (45) 0.01 (10) 0.01 (46) 0.01 (11) 0.01 (47) 0.01 (12) 0.01 (48) 0.01 (13) 0.01 (49) 0.01 (14) 0.01 (50) 0.01 (15) 0.01 (51) 0.01 (16) 0.01 (52) 0.01 (17) 0.01 (53) 0.01 (18) 0.01 (54) 0.01 (19) 0.01 (55) 0.01 (20) 0.01 (56) 0.01 (21) 0.01 (57) 0.01 (22) 0.01 (58) 0.01 (23) 0.01 (59) 0.01 (24) 0.01 (60) 0.01 (25) 0.01 (61) 0.01 (26) 0.01 (62) 0.01 (27) 0.01 (63) 0.01 (28) 0.01 (64) 0.01 (29) 0.01 (65) 0.01 (30) 0.01 (66) 0.01 (31) 0.01 (67) 0.01 (32) 0.01 (68) 0.01 (33) 0.01 (69) 0.01 (34) 0.01 (70) 0.01 (35) 0.01 (71) 0.01 (36) 0.01 (72) 0.01
44 ppm ppm ppm 1/ / / / / / / / / / / / / / / / / / / /
45 No. 17 ppm ppm ppm 1/
46 1 3
47 ,207 10, ,261 10,360 ph ( 4) , ,207 10, N,N-N,N
48 1 3 6 N,N ph COD DO SS
49 , , , ,564
50
51 (22) PCR ELISA4 2 A SFTS PCR D68PCR A 8 5 SFTS D
52 () ()
53 ,360 2, ,902 2, % 1 2.0% 3 8.6% IgG25 25 IgM
54 (139) 52 ( 14) 40 ( 5) 83 (17) 97 (18) 76 (13) 96 (25) 57 (14) 55 ( 6) 30 ( 5) 43 ( 5) 55 ( 5) 65 (12) 227 ( 8) 12 ( 4) 49 ( 1) 35 ( 3) 288 ( 13) 1,360 (168) 11 ( 0) 0 ( 0) 4 ( 0) 1 ( 0) 28 ( 0) 96 (14) 16 ( 0) 0 ( 0) 4 (0) 1 ( 0) 20 ( 0) 81 ( 5) 7 ( 0) 3 ( 0) 4 ( 0) 4 ( 0) 28 ( 1) 129 (18) 27 ( 1) 2 ( 1) 5 ( 0) 2 ( 0) 20 ( 0) 153 (20) 13 ( 0) 0 ( 0) 4 ( 0) 2 ( 0) 26 ( 2) 121 (15) 86 ( 6) 0 ( 0) 4 ( 0) 6 ( 2) 22 ( 0) 214 (33) 17 ( 0) 3 ( 2) 4 ( 1) 2 ( 0) 27 ( 2) 110 (19) 15 ( 0) 0 ( 0) 4 ( 0) 1 ( 0) 19 ( 0) 94 ( 6) 2 ( 1) 0 ( 0) 4 ( 0) 0 ( 0) 25 ( 2) 61 ( 8) 18 ( 0) 2 ( 0) 4 ( 0) 0 ( 0) 25 ( 3) 92 ( 8) 12 ( 0) 0 ( 0) 4 ( 0) 2 ( 0) 27 ( 1) 100 ( 6) 3 ( 0) 2 ( 1) 4 ( 0) 14 ( 1) 21 ( 2) 109 (16)
55 PCB ,217 PCB ,360 1/2% 5/2% No.22,4,5,2',4',5'-hexachlorobiphenyl ) No.12,4,5,3',4'-pentachlorobiphenyl ) 2No.2 No.5 (2,3,4,5,3',4'-hexachlorobiphenyl )% LC/MS GC/MS LC-MS/MS 15 4, , PCB PCB ppb 0.30ppb GC-MS/MS GC-MS/MS LC-MS/MS 50%200%RSD30
56 1 3 2 No SEMEDSFT-IR ph SEMEDSFT-IR SEMEDS FT-IR SEMEDSFT-IR LAS FT-IR SEMEDSFT-IR ph FT-IR SEMEDS SEMEDS SEMEDS No , , ,217
57 1 3
58 1 3 No O157,O Unicapsula seriolae PM
59 1 3 No. GC-MS/MS PCR IC NS1 ELISA IgM PCR 10 IC NS1 14 ELISA IgM PCR 23 PFOS PFOA PFOSPFOA PFOS PFOA ,894kg 18 LAS LAS A
60 1 3C11C12C10C13 C PM2.5 PM PM PM2.5 SO 2-4 OC NH + 4 NH + 4 SO 2-4 NO %1 10 O157,O O157 O26 O ESBL TEM+CTX-M-1 1 O ESBL CTX-M-1 1 Kudoa septempunctata Unicapsula seriolae U.seriolae Percoll PBS 0.1Tween80 PBS 1500rpm rpm copy/g Tween /g 1.8 Percoll 1.2 Tween copy/g, /gk.septempunctata copy/g /g 23 7 PM2.5 PM PM PM2.5 SO 2-4 OC NH + 4 NH + 4 SO 2-4 NO %1 27
61 1 3 QuEChERS QuEChERS 58% 2 79% LAS 25 LAS A C11-LAS 40% LAS BHCDDT 129
62 1 3
63 N,N- Environmental Survey and Monitoring of Chemicals in FY2015 (N,N-Dimethylacetamide) Sayaka YAMASHITA and Tomoko TANABE Environmental Science Section, Fukuoka City Institute of Health and Environment 27 N,N- 26 C-4 N,N- Key Words Environmental Survey and Monitoring of ChemicalsN,N- N,N-Dimethylacetamide GC-MS 27 N,N- N,N PRTR 475,787684,064kg/ 90% 1 PRTR N,N- POPs 1,876kg/ 17 8
64 1 3 1 N,N- C 4 H 9 NO CAS ,000,000 mg/l hpa N,N C N,N- -d 9 CDN isotopes 4- PCB PCB PCB Waters Sep-Pak AC-2 GC-MS GC Agilent 6890NMS JMS-Q1000GC GC-MS 2 2 GC-MS ThermoFisher Scientific TR-WAX 30m0.25mm, 0.25m 50 (5min)-3 /min-80-5 /min /min-200 (3min) min 1 L He (1.0 ml/min) (m/z) (m/z) N,N N,N--d mL N,N- -d 9 2.0mg/L 50L 10mL 10mL 10mL Waters Sep-pak AC-2 20mL 10mL 3000rpm20min 30min 2mL 4-2.0mg/L 50L 2.0mL GC-MS 2
65 1 3 N,N- 0.15g/L GC-MS 7 MDL MQL MDL 0.012g/LMQL 0.031g/L g/L 7 GC-MS IDL IDL IDL 0.63g/L 4 (MDL) (MQL) MDL MQL (g/l) (%) (g/l) (g/l) (%) n=7 N,N- m/z 87 3 (IDL) IDL IDL S/N (g/l) (%) (g/l) (g/l) n=7 N,N- m/z 87 3 IDL 4 MDL N,N- 0.30g/L GC-MS 5 5 N,N- 100% 100% 5 (g/l) (%) (%) (%) n=7
66 1 3 N,N- m/z 87 5 C-4 N,N- 6 N,N- m/z 87 6 C-4 26 PRTR N,N N,N- C-4 N,N- N,N- C-4 N,N- N,N PRTR
67 Evaluation of River Environment by Bottom Fauna in Fukuoka City (Mikasa Riverin 2015) Tetsuya SHIMIZUChiho NITTA and Kazuyuki UEO Environmental Science SectionFukuoka City Institute of Health and Environment ASPT ASPT Key Words freshwater area bottom fauna Mikasa River (868.7m) 20.7km ASPT
68 mL 70 3 phdobodsst-nt-pec 7 ASPT ASPT 1 10 TS ASPT ASPT=TS/n TS: n: ASPT ASPT ASPT 5 m 2m 20 25cm 5765cm/s
69 ASPT 7.4 km 48m. 2028cm 1922cm/s /3 16 ASPT 7.3 km 28m. 1015cm ASPT 7.0 km 25m cm 4256cm/s , ASPT 6.0 km 17m. 820cm 40 51cm/s ASPT 5.5 ASPT ASPT 6 BOD BOD mg/L mg/l mg/l mg/L T-NT-P 2015 ASPT 8ASPT 9 ASPT ASPT 5.5 ASPT ASPT 7.4 ASPT ASPT 7 BOD mg/L 0.3mg/L T-N mg/L T-P mg/L 5 ph DO 10mg/L DOBODT-NT-P 10 BODT-NT-P 1995 BOD T-NT-P ASPT 2000 ASPT ASPT
70 ASPT
71
72 ASPT
73 1 3 5 ASPT ASPT (2015 ) ASPT ph DO (mg/l) BOD (mg/l) SS (mg/l) T-N (mg/l) T-P (mg/l) EC (ms/m)
74 ASPT
75 1 3
76 Heat Stroke Outbreak Risk Investigation in Indoors Ryoichi OHIRA, Eisuke UNO and Ayako NAKASHIMA Environmental Science Section, Fukuoka City Institute of Health and Environment WBGTWBGT WBGT WBGT Key Words heat stroke WBGT wet bulb globe temperature WBGT WBGT WBGT B 1 2 WBGT WBGT WBGT WBGT
77 WBGT WBGT 3 WBGT WBGT WBGT WBGT WBGT 25 WBGT 3 WBGT 4 WBGT WBGT WBGT WBGT WBGT WBGT 5 WBGT WBGT WBGT WBGT WBGT WBGT WBGT WBGT WBGT 5 WBGT WBGT WBGT WBGT 25
78 1 3 PM Monitor Investigation on Yellow Sand and PM 2.5 in Fukuoka City in 2013 Sayaka YAMASHITA, Keiko NAKAMUTA and Hiromi HAYASHI Environmental Science Section, Fukuoka City Institute of Health and Environment Environmental Conservation Section, Environmental Bureau Recycling-Based Society Planning Section PM PM PM PM 2.5 PM 2.5 PM 2.5 PM AlCaTi PM 2.5 SO 2-4 NO - 3 NH + 4 Pb Key Words monitor investigation yellow sand PM 2.5 allergy Pollen Componential analysis PM PM PM 2.5 PM m PM
79 1 3 PM PM PM PM PM PM PM % PM 2.5 PM2.5 PM2.5 LV-50 FRM-2000 Thermo Scientific PTFE Whatman Pall PM 2.5 PTFE % 24 1cm 2 Sunset Laboratory OCEC 1/4 10mL m PTFE Dionex ICS SO 2-4 NO - 3 Cl - NH + 4 Na + K + Ca 2+ Mg 2+ 8 Si PTFE 1/2 Perkin Elmer Multiwave3000ICP-MSAgilent 7700x NaAlKCaSc TiVCrMnFeCoNiCuZnAsSeRb MoSbCsBaLaCeSmHfTaWPbTh 29 Si PTFE X EDX-900HS 35 g/m 3 PM 2.5 PM 2.5 PM 2.5 PM 2.5 PM 2.5 PM ,974 1,1391,784 1,000 1
80 % % % % % %60 9.4% PM % 46.4% 36.9% 22.5% % km10 5 km19 7 km20 4 km 8 PM g/m PM g/m PM /4 3/5 3/8 3/9 3/19 3/20 5/9 5/12 5/13 5/14 5/21 5/22 5/23 5/24 5/25 5/29 5/30 PM ]
81 /cm PM PM ,500 PM % 6 7 PM PM 2.5 PM PM PM PM 2.5 PM 2.5 PM 2.5 4m PM m PM 2.5
82 PM PM 2.5 PM %PM % PM 2.5 PM PM2.5 PM 2.5 PM 2.5 PM g/m PM g/m 3 PM % 17.6% 4.2% SO %NH 4 25%NO %
83 1 3 PM PM % 9.2% Ca g/m g/m SiAlTi 1, ng/m Fe 440ng/m ] SO 4 NH 4 NO 3 Ca 2+ PM PM PM 2.5 SO 2-4 NO NH g/m 3 23 PM 2.5 Ca 2+ SiAlTi NiSe 3 PM 2.5 PbZnAs 3 1) PM PM ) ) PM 2.5 PM 2.5 1) PM 2.5 Ca 2+ 2) PM 2.5 SO 2-4 NO - 3 NH + 4 Pb 3 [ng/m 3 ] Si Al Ti Fe Ni Se Pb Zn As PM 2.5 1, PM
84 1 3 1) tml ) ) ) PM 2.5 PM )2013 6) PM ) PM ), Medicine 28 (8) : ) hikousakentouiinkai.html ) 25 ikityosahokokusyo.pdf ) ) ) ) ) ) PM ) 2013
85 * * Weight Survey According to Household Garbage Composition ( ) Takuro OKAMOTO*, Keisuke MOCHIDUKI, and Kenichi NONAKA Health and Environment Management Section, Fukuoka City Institute of Health and Environment *Animal Welfare and Management Center, Environmental Health Department, Public Health & Welfare Bureau Key Words household garbage composition, weight survey, Fukuoka city, municipal solid waste, charge for garbage
86 /45L 1 45 /45L 1 22 /45L 2 9 H17.7 H18.1H18.1 H H H16.12 H23.12 H14 320,382 t H27 310,000 t H21 284,300 t H37 268,200 t kg ,000kg 21
87 g / 1,401, ,202 19,669 3, , ,142 49, ,414, ,162 16,408 3, , ,636 54, ,427, ,420 15,295 3, , ,515 53, ,438, ,321 14,108 3, , ,040 52, ,452, ,306 13,819 3, , ,189 49, ,463, ,589 12,179 3, , ,375 40, ,479, ,830 11,647 3, , ,370 39, ,492, ,901 11,709 3, , ,477 38, ,506, ,848 12,579 3, , ,125 37, ,519, ,622 14,184 3, , ,330 36, ,531, ,195 14,331 4, , ,668 34, / /
88 ,
89 , g/ g/ ( () () ()
90 t/ ( () 101, ,258 99, ,182 93,825 96,940 95,875 95,436 97,322 99, ,699 5,242 3,922 5,763 5,136 4,277 4,249 4,875 2,991 3,806 2,696 3,797 15,434 14,568 14,270 16,490 15,504 16,201 16,521 13,867 14,136 14,560 14, ,747 1,681 1,647 1,622 1,337 1,859 1,625 1,631 1,631 1,618 1,085 9,610 12,327 8,233 9,732 9,088 7,702 6,500 4,894 6,796 8,358 5,424 25,043 29,137 31,284 31,087 23,256 28,684 26,271 26,374 26,913 26,153 20,882 43,972 40,343 38,419 37,845 40,096 37,979 39,812 45,407 43,768 45,836 56,138 () 50,087 48,469 48,297 47,847 45,710 47,276 50,644 45,951 51,108 49,071 49,902 1,165 1,121 1,372 1,622 1,337 1,594 1,625 1,631 1,631 1,348 1, , , ,698 6,444 6,586 5,677 5,346 5,843 5,417 5,166 5,165 4,853 4,882 26,499 25,495 24,423 25,140 25,127 26,028 27,895 24,743 27,185 28,310 28,476 1,747 1,681 1, ,062 1, ,085 13,104 12,607 13,721 13,786 12,029 11,952 13,812 13,051 15,767 13,212 13,560 16,016 12,887 13,172 14,868 15,236 14,873 19,500 18,489 22,563 15,638 16,543 15,725 18,211 15,916 17,841 12,563 10,358 14,354 13,867 16,583 11,055 16, ,503 92,453 93,577 84,610 97,032 92,691 86,666 95,165 79,380 88,706 83,257 () 5,533 5,883 3,567 2,974 2,941 3,453 3,791 2,992 4,893 5,662 2,984 2,330 1,961 1,372 1,352 1,337 1,594 1,625 1,088 1,903 1,887 1,085 1,165 1, , ,359 1, ,038 2,521 1, ,062 1,083 1,360 1,631 2,427 1, g 5
91 g/
92 () () () () t/ () ,360 1,608 1,377 1,312 1,202 1,133 1, ,069 1, () 5,448 4,890 5,047 4,698 4,298 4,129 4,414 4,180 4,466 4,624 4, ,717 2,937 3,426 3,104 3,303 3,629 3,774 3,688 3,245 3,489 3, ,198 1,269 1, ,220 1,092 1,060 1, () 2,950 2,428 2,004 1,933 2,322 2,399 2,667 2,506 2,239 2,099 2, ,006 1,739 1,545 1,735 () 6,864 5,480 5,185 4,895 4,657 3,276 2,434 2,927 3,547 4,581 4, , , ,311 4,250 3,808 3,612 3,413 1, ,229 1,623 2,057 2,451 1,633 1,510 1, , ,447 1,
93 / 11 2, g/ g/
94
95 , g/24
96 ) ) ) 3R 3R Q&AH17~H26 1)
97 1 3 Research for the accurate grasp of number of measles patients - Detection of the virus from measles suspected cases - Hideomi FURUKAWA, Takahisa MATSUFUJI and Mamoru MIYASHIRO Health Science Section, Fukuoka City Institute of Health and Environment MV PCR 7 RV B19PVB HHV PCR RV36 PVB1928 HHV37 MV 7 MV B3 D9 IgM PCR Key WordsMV measles virus PCR polymerase chain reaction genotype IgM IgM antibodies WHO 2, WHO 2 measles elimination 3) 20 MV 100 Paramyxovirus Morbillivirus nm RNA MV A H clade genotype ), 2) D3D5 A 1) )
98 WHO PCR MV RV B19PVB HHV PCR IgM % PCR UR NP BL URNPBL ,750 G3,000 rpm G1,500 rpm 10 1mL 2mL PBS Ficoll-paque mL 1,300 G 20 PBS 2 PBMC PBMC PBS MEM 1,300 G2,500 rpm QIAamp Viral RNA mini kit RNA Invitrogen SuperScript 0 3 III Reverse Transcriptase RNA 15 DNase/RNase free water X First-Strand Buffer mM dntps M DTT 1.5 Random Primer /) 0.75 RNase inhibitor38 unit/ 1.0 SuperScript 0 3 III cdna crt-pcr PerfectShot 0 3 Ex TaqLoading dye mix N HA NS RT-PCR nested-pcr
99 1 3 PCR N 450 ABI PRISM 310Applied Biosystems Neighbor-joining (NJ) 3 3 MVRV crt-pcr MV (N) 1st PCR MV (N) Nested PCR MV (HA) 1st PCR MV (HA) Nested PCR RV 1st PCR RV Nested PCR pmvgtf1m CGR TCT TAC TTY GAT CCR GC pmvgtr1 TTA TAA CAA TGA TGG AGG pmvgtf2m AGA YTA GGR CAR GAG ATG GT pmvgtr2 GAG GGT AGG CGG ATG TTG TT MHL1 MHR1 MHL2 MHR2 AAC GGA TGA TCC AGT GAT AG TTG AAT CTC GGT ATC CAC TC TAC CTC TCA TCT CAC AGA GG CAC CTA AGG CTA GGT TCT TC NSL F3 TCC TTG CGC CGA AGA CT NSL B3-6 AGA GGG GGT CCA CTT GAG F2 nest B2 nest CCA CTG AGA CCG GCT GCG A GCC TCG GGG AGG AAG ATG AC RVPVB19HHV PCR Multiplex PCR Assay Kit PCR 1 PCR 4 5 PCR cdna 5 1st PCR nested-pcr 1st PCRnested PCR PCR PCR DNase/RNase free water RVForwardReverse 1.0 PVB19ForwardReverse 0.4 HHVForwardReverse 0.4 Mix Mix PCR HHV HHVF ATA ATT GGC AAT GAA CAC CGT T 1st nested 762 bp HHVR GAT CCT TTT TGA GAT GCC CAA GG RV 1st 513 bp PVB19 1st 242 bp RV nested 363 bp E1P5 E1P8 GCC ATG CTA CCG TCG AAA TGC C GCA CAG CAA GCG AGT AAG CCA G PVB-1 CAC TAT GAA AAC TGG GCA ATA AAC PVB-2 AAT GAT TCT CCT GAA CTG GTC C E1P6 E1P7 GGC TGA AGT TCA AGA CAG TTC GC GCA GCC CAC TCC GCC CAG GTC PVB19 PVB-3 ATA AAC TAC ACT TTT GAT TTC CCT G nested 218 bp PVB-4 TCT CCT GAA CTG GTC CCG Temperature Time Cycle sec sec. 90 sec. 90 sec. 10 min. Hold 27 3 crt-pcr rt RT-PCR real-time RT-PCR MV RV crt-pcr rt RT-PCR MV RV rt RT-PCR 40 1
100 MVRV TaqMan Fast Virus 1-Step Master Mix Mx3005P PCR 7 Ct Ct Ct Ct 40 Ct N PCR 8 MV PCR PCR PVB19 PCR HHV PCR HHV PCR 19 MV PCR 7 PCR 1B3 4 D9 3 crt-pcr PCR 8 PCR URNPBL IgM EIA PCR IgM EIA IgM UR NP BL UR NP BL EIA IgM MV RV PVB MV crt-pcr HHV MV rt RT-PCR 7 rt RT-PCR MV Forward Primer MVN1139F TGG CAT CTG AAC TCG GTA TCA C MV Reverse Primer MVN1213R TGT CCT CAG TAG TAT GCA TTG CAA MV Probe MVNP1163P FAM - CCG AGG ATG CAA GGC TTG TTT CAG A - TAMRA RV Forward Primer NS(32-54)Fwd CCT AHY CCC ATG GAG AAA CTC CT RV Reverse Primer NS( )Rev AAC ATC GCG CAC TTC CCA RV Probe NS (93-106) Probe FAM - CCG TCG GCA GTT GG - MGB
101 1 3 MV PCR URNPBL UR NP BL IgM 56 IgM 19 IgM 3 IgM 4 1 IgM MV PCR MV 1 6
102 1 3 9 D9 MV PCR IgM / / / 56 IgM MV PCR 12 RV PCR 2 PVB19 PCR 4 HHV PCR 4 IgM PCR IgM PCR IgM MV PCR MV B3 D9 1 D3 D5 RV 1 6 RV PCR RV PVB19 PCR 7) HHV 8) PCR HHV RV 36 IgM MV PCR IgM MV PCR 12 RV IgM 5 PCR 102 MV PCR 95 PCR MV PCR RV PCR MV PCR 1 es.html pp B19 IASR Vol. 37 p. 9-10: Human herpesvirus 6 (HHV-6) Human herpesvirus 7 (HHV-7)
103 LC-MS/MS Analysis of Medicinal Substances in Health Foods by LC-MS/MS Akemi MUTA, Hironori TOWATARI, Michihiko MIYAMOTO, Megumi KAWASAKI and Etsuko MIYAZAKI Health Science Section, Fukuoka City Institute of Health and Environment N- 7 LC-MS/MS 6 65%~124% 27 Key Words LC-MS/MS medicinal substance health food 1) 25 LC-TOFMS 2) 4 3 N- 7 LC-MS/MS 6 M.W Toronto Research Chemicals. Inc M.W Toronto Research Chemicals. Inc
104 M.W Toronto Research Chemicals. Inc M.W M.W N- M.W M.W ~100 mg/l 5 mg/l HPLC HPLC HPLC US-5 H-103NR Agilent HP 1200 AB SCIEX 4000QTRAP 5 ml 50 ml rpm5 25 LC-MS/MS Table 1 Column Column temp. Mobile phase Table 1 LC-MS/MS condition for analysis of medicinal substances Waters Atlantis T3 35 A: 0.02%HCOOH B: 0.02%HCOOH in CH 3CN Gradient profile B: 0%(0 min)0%(0 min)80%(10 min) 80%(20 min)0%(20.1 min)0%(30 min) Flow rate Injection volume Ionization IS TEM Moitor ions 0.2 ml/min ESI, positive 5500V 600 Precursor ions Product ions CE CXP (m/z) (m/z) Sildenafil Vardenafil Tadalafil Xanthoanthrafil Fenfluramine N-nitrosofenfluramine Sibutramine mg 5 ml 5 10 ml rpm mg/l Fig ~0.1 mg/l ~1.000 Table 2 Recovery of medicinal substances from health foods Recovery(%) No.1 No.2 No.3 No.4 No.5 No.6 average Medicinal Substances contents capsule base contents capsule base Sildenafil Vardenafil Tadarafil Xanthoanthrafil Fenfluramine N-nitrosofenfluramine Sibutramine
105 mg/kg Table 2 65%~124% LC-MS/MS 27 1) HP 2) Sildenafil Vardenafil Tadalafil Xanthoanthrafil Fenfluramine N-nitrosofenfluramine Sibutramine Fig.1 Chromatograms of standard solutions of medicinal substances(0.05 mg/l)
106 Validation of Simultaneous Analysis of Elements in Mineral Water Hironori TOWATARI and Etsuko MIYAZAKI Health Science Section, Fukuoka City Institute of Health and Environment BCr MnCuZnAsSeCdBa Pb % RSD% RSD% Key Words mineral water elements validation ICP-MS vol% 1 ICP-MS 10 ADVANTEC ULTRAPURE WATER BCrMnCu SYSTEM cmtoc<1 ppb Zn As Se Cd 1.38 BaPb 10mL 1,000mL 2 B CrMnCuZnAsSeCdBaPb 1,000 mg/l Be CoGaIn TlY 1,000mg/L
107 B Be 100ng/mL Co 10ng/mL Ga 10ng/mL Y 1ng/mLIn 1ng/mLTl 10ng/mL mL 0.40mL 40mL 2 ICP-MS 2 ICP-MS ICP-MS He 3 ICP-MS ICP-MS Ba 9 Ba Ba 1
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109 GC-MS/MS Validation on Quick Analytical Method of Pesticide Residues in Milk by Gas Chromatography with Tandem Mass Spectrometry Masatoshi FUJII, Yukiko KATOU and Junko TSUNEMATSU Health Science Section, Fukuoka City Institute of Health and Environment BHCDDT 129 QuEChERS QuEChERS 58% 2 79% Key Words milk pesticide residue quick analytical method validation gas chromatography with tandem mass spectrometry(gc-ms/ms) PL2005 GC/MS GC/MSMix 7 20ppm QuEChERS Dr.Ehrenstorfer GmbH Riedel de Ha 0 5n mg/L 1mg/L n-1:1 C18 InertSep C181g/6mL 10mL -N- PSA
110 BOND ELUT LRC-PSA500mg n-1:1 10mL QuEChERS CH 4g 1g 1g 0.5g QuEChERS CHPSA400mgC18 400mg 1200mg Captiva ND Lipids Captiva ND Lipids 5A 451GC Scion TQ 250 DB-5MS+DG0.25mm i.d.30m0.25m 501min25/min12510/min 300 1mL/min 2L EI70eV mg/L 1 2
111 BHCDDT ppm ppm ppm 1/ % RSD<25%RSD<30% 0.005ppm S/N 10 BHCDDT 3 TIC ppm % 2 78% 79% % 120 BHCDDT ppm 128
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116 Studies on Daily Intake of Pesticides in Foods in Fukuoka City (2015) Saharu IBE, Natsumi MIYACHI and Junko TSUNEMATSU Health Science Section, Fukuoka City Institute of Health and Environment LC/MS GC/MS LC-MS/MS GC-MS/MS 2 ADI ADI Key Words pesticide daily intake ADI LC-MS/MS GC-MS/MS 27 LC-MS/MS GC-MS/MS 62 ADI 2022 ADI )
117 ) (g)* * mL 35 PL2005 LC/MS MIX g/mL 0.5mol/L 52.7g 30.2g 500mL 1mol/L ph 7.0 1L C18FF/C18 FF/DRY InertSep C18FF1g/3g 10mL / GC/NH 2 InertSep GC/NH 2 1g/1g 3:1 10mL 3 PL2005 GC/MS MIX 20g/mL 1: mol/L LC-MS/MS C18 FF/DRY LC-MS/MS GC/NH 2 LC-MS/MS 300 Agilent 1260 MS/MS)ABSCIEX TQ Bruker Daltonics scion TQ 67
118 1 3牽 偏隠h冩,41,2016 燕 2 { 黨ra (LC-MS/MS) ra兆 * 麼な喘余 ra方 アセタミプリド, アルドキシカルブ, イミダクロプリド, カルバリル, クロキントセ 恰 18 ットメキシル, クロチアニジン, ジノテフラン, チアクロプリド, チアメトキサム, テブフェノジド, トリフルムロン, ノバルロン, ピリミカルブ, フェノブカルブ, フルフェノクスロン, ヘキシチアゾクス, メソミル, メトキシフェノジドアニロホス, インダノファン, オキサジクロメホン, クロリダゾン, クミルロン, 茅課 16 クロロクスロン, ジウロン, テブチウロン, ピリフタリド, フェノキサプロップエチル, フルフェナセット, フルリドン, ベンゾフェナップ, メタベンズチアズロン, モノリニュロン, ラクトフェンイプロバリカルブ, エポキシコナゾ`ル, カルプロパミド 3 * 和 があるもの ( ra ) は峠撹 28 定 5 埖 r泣において忽坪で鞠hがあるraを幣す. 燕 3 { 黨ra (GC-MS/MS) ra兆 * 麼な喘余 ra方 EPN, イソフェンホス, イソプロカルブ, エトプロホス, エトリムホス, 恰 10 クロロベンジレ`ト, フェンチオン, フェンバレレ`ト, フルシトリネ`ト, マラチオンアラクロ`ル, シハロホップブチル, ジメテナミド, シメトリン, チオベンカルブ, 茅課 13 ピリブチカルブ, ピリミノバックメチル E, ピリミノバックメチル Z, ピラフルフェンエチル, ブタクロ`ル, メトラクロ`ル, メフェナセット, レナシルチフルザミド, トルクロホスメチル 2 * 和 があるもの ( ra ) は峠撹 28 定 5 埖 r泣において忽坪で鞠hがあるraを幣す. 燕 4 LC-MS/MS の訳周 99匣悶クロマトグラフ 蛍裂カラム Waters 芙u Atlantis T3 C18 ( 2.1mm i.d 50 mm,3.0μm) カラム梁業 40 卞 嗜 A 匣 :5 mmol/l 瓮 鵐皀縫Ε B 匣 : アセトニトリル 卞 嗜狒楚 0.2 ml /min グラジエント訳周 0% B (0 min) 0% B (1 min) 90% B (20 min) 90% B(30 min) 0% B (30.1 min) 0% B (40 min) 廣秘楚 5μL 99 楚蛍裂 イオンスプレ` Rイオンソ`ス梁業 ( ポジティブ y協 ) 5.500V 650 ( ネガティブ y協 ) V
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120 1 3 7 GC-MS/MS No. Q1(m/z) Q3(m/z) CE(eV) No. Q1(m/z) Q3(m/z) CE(eV) 1 EPN E Z LC/MS 3) GC/MS 3) C18/DRY C18 FF/DRY 4) 10 5g10 ml25 ml 50 ml 20 ml 100 ml g 20mL0.5mol/L 20mL 10g LC-MS/MS 5L GC-MS/MS 2L 0.01g/g ppm
121 g/g 25 PEG ppm g/g0.017g/g 50kg ADI 10 ADI ADI LC-MS/MS GC-MS/MS (ADI) ADI
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135 ph ph ph2 ph ph , km 130m202 13km 170m300m263 8/31010/ ph ph mm ms/m mmol/m 2 S/cm mm ms/m mmol/m 2 S/cm 4/ 6-4/ / 5-10/ /13-4/ /13-10/ /20-4/ /19-10/ /27-5/ /26-11/ / 7-5/ / 2-11/ /11-5/ / 9-11/ /18-5/ /16-11/ /25-6/ /24-11/ / 1-6/ /30-12/ / 8-6/ / 7-12/ /15-6/ /14-12/ /22-6/ /21-12/ /29-7/ /28-1/ / 6-7/ / 4-1/ /13-7/ /12-1/ /21-7/ /18-1/ /27-8/ /26-2/ / 3-8/ / 1-2/ /10-8/ /8-2/ /17-8/ /15-2/ /24-8/ /22-2/ /31-9/ /29-3/ / 7-9/ / 7-3/ /14-9/ /14-3/ /24-9/ /22-3/ /28-10/ /28-4/
136 1 3 ph 7/278/1010/ mm ms/m mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 mmol/m 2 4/ 6-4/ /13-4/ /20-4/ /27-5/ / 7-5/ /11-5/ /18-5/ /25-6/ / 1-6/ / 8-6/ /15-6/ /22-6/ /29-7/ / 6-7/ /13-7/ /21-7/ /27-8/ / 3-8/ /10-8/ /17-8/ /24-8/ /31-9/ / 7-9/ /14-9/ /24-9/ /28-10/ / 5-10/ /13-10/ /19-10/ /26-11/ / 2-11/ / 9-11/ /16-11/ /24-11/ /30-12/ / 7-12/ /14-12/ /21-12/ /28-1/ / 4-1/ /12-1/ /18-1/ /26-2/ / 1-2/ /8-2/ /15-2/ /22-2/ /29-3/ / 7-3/ /14-3/ /22-3/ /28-4/
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（別添）安全性未審査の組換えDNA技術応用食品の検査方法_NIHS 最終版_YF
コムギ (MON71200 MON71100/71300 MON71700 MON71800) の検査方法 コムギ穀粒又は粉砕加工食品を検査対象として 1 検体から 2 併行で DNA を抽出精製する 得られた各 DNA 試料液を用いて 2 ウェル併行で定性リアルタイム PCR を実施する 1. DNA 抽出精製 1.1. 試料の洗浄 粉砕 ( 穀粒の場合 ) コムギ穀粒を試料 ( 重量 ) あたり
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平成26年度 化学物質分析法開発報告書
2,2-2,2 -Iminodiethanol Diethanol amine CAS 111-42-2 C 4 H 11 NO 2 105.1356-105.0790-28 C 1) 1.0881 g/cm 3 1)
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