QIAGEN Product News 1May 2014 Pick Up n QIAxpert n REPLI-g Single Cell DNA/RNA Sample & Assay Technologies
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- ゆき ちゅうか
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1 QIAGEN Product News 1May 214 Pick Up n QIAxpert n REPLI-g Single Cell DNA/RNA Sample & Assay Technologies
2 Pick Up Get True Insight into Your Sample Quality QIAxpert QIAxpert DNA RNA DNA RNA QIAxpert 2 16 QIAxpert QIAxpert QIAxpert DNA RNA 2 µl 16 2 PC QIAxpert QIAxpert 2 µl 2 QIAxpert QIAxpert 2 QIAGEN Product News Issue 1/214
3 Pick Up DNA RNA QIAGEN * * A Absorbance (1 mm) B Absorbance (1 mm) Wavelength (nm) QIAxpert QIAxpert Export USB QR 2 HTML CSV Microsoft Excel A 26 A 26 /A 28 A 26 / A 23 HTML Wavelength (nm) RNA A B RNA gdna RNA DNA B RNA Cat. no. QIAxpert Instrument QIAxpert QIAxpert Slide-4 (25) 1 2 * ,45, , * QIAxpert Slide-4 (25) Cat. no. 997 QIAxpert QIAGEN Product News Issue 1/214 Sample & Assay Technologies 3
4 Pick Up Compare to our current method... the high quality results obtained... Luting Song, BGI, China ゲノムカバー率 (%) % REPLI-g Single Cell Kit (1 cell) REPLI-g Single Cell Kit (1 cell) 97.39% 99.96% REPLI-g Single Cell Kit (1 cells) gdna REPLI-g Single Cell Kit (1 cells) gdna エラー率.43%.22%.35% キメラ.8%.6%.11% インデル率.5%.2%.1% DNA REPLI-g Single Cell DNA NGS Illumina MiSeq DNA REPLI-g Single Cell Kit 2 µg DNA 3 bp 1 µg A gdna REPLI-g Single Cell DNA * B DNA REPLI-g DNA * Burrows-Wheeler Alignment cut-off 1x coverage bio-bwa.sourceforge.net REPLI-g Single Cell DNA REPLI-g Single Cell Kit WGA MDA 4 µg >1 kb DNA UV DNA REPLI-g Single Cell Kit 1, 1 DNA DNA I have tried the kit and successfully performed single cell whole genome amplification. The DNA obtained allowed me to reliably detect allele-specific point mutations in a colorectal adenocarcinoma cell line. The excellent yield and sequence representation of the amplified DNA really helped me to advance my work in single-cell genomics. Dr. Masafumi Muratani Research Associate, Cancer Therapeutics & Stratified Oncology, Genome Institute of Singapore (GIS) REPLI-g Single Cell Kit (24) ,5 REPLI-g Single Cell Kit (96) , QIAGEN Product News Issue 1/214
5 Pick Up REPLI-g WTA Single Cell Kit RNA mrna MDA WTA NGS RNA mrna poly A + RNA REPLI-g WTA Single Cell Kit 1 1, REPLI-g RNA RNA in vivo REPLI-g WTA Single Cell Kit (24) , RPKM (3 cells) RPKM (average, 1 5 cells) 1 rrna mrna poly A + 3 R 2 1 = REPLI-g WTA Single Cell cdna Covaris S22 GeneRead Library Prep I Kit NGS MiSeq Instrument Illumina RNA Bowtie2 RNA RPKM reads per kilobase and million mapped reads 3 WTA 1 5 RPKM REPLI-g Cell WGA & WTA Kit DNA RNA mrna MDA gdna RNA NGS REPLI-g Cell WGA & WTA Kit WGA WTA 25 1 REPLI-g DNA RNA mrna poly A + REPLI-g SensiPhi DNA Polymerase PCR 1, * RNA mirna poly A + WGA (amplify DNA) Repair Ligation Whole genome amplification REPLI-g Cell WGA & WTA Procedure Cell sample (25 1 cells) Cell lysis Add WGA Ready Mix Add ligation mix Add REPLI-g SensiPhi amplification mix 6 min at 42 C 3 min at 95 C 3 min at 24 C 5 min at 95 C 2 h at 3 C 5 min at 65 C Add Lysis Buffer 5 min at room temperature 3 min at 95 C WTA (amplify RNA) gdna removal Reverse Transcription Ligation Whole transcriptome amplification Add gdna Wipeout Buffer 1 min at 42 C Add Quantiscript RT mix and primers* Add ligation mix Add REPLI-g SensiPhi amplification mix REPLI-g Cell WGA & WTA Kit (12) , Amplified gdna Amplified cdna QIAGEN Product News Issue 1/214 Sample & Assay Technologies 5
6 Sample Technologies Sample collection Purification Amplification cdna synthesis Analysis Sample Technologies QIAcube HT System 96 DNA RNA mirna QIAcube HT 96 DNA RNA mirna QIAcube HT QIAGEN QIAcube HT QIAcube HT Kits QIAcube HT QIAcube HT system, Priority* ,9, * 3 QIAcube HT system, PrioPLUS Cat. no QIAGEN Product News Issue 1/214
7 QIAamp 96 DNA QIAcube HT Kit DNA QIAcube HT M 5 µl 1 µl 15 µl 2 µl DNA QIAamp 96 DNA QIAcube HT Kit DNA 96 QIAamp DNA QIAamp 96 DNA QIAcube HT Kit QIAcube HT µl DNA 2 µl DNA 7 µl.8 1x TBE M HindIII Sample Technologies QIAamp 96 DNA QIAcube HT Kit (5) , RNeasy 96 QIAcube HT Kit QIAcube HT RNA RNA mirna RNA RNA RNA RNeasy 96 QIAcube HT Kit QIAcube HT RNA mirna RNA 96 RNeasy RNA yield (µg) Starting material (mg) 2 25 RNA RNAlater mg RNeasy QIAcube HT Kit RNeasy 96 QIAcube HT total RNA Tissue V1.QSP Protocol QIAcube HT RNA RNA 1 µl RNase RNA RNeasy 96 QIAcube HT Kit (5) , QIAGEN Product News Issue 1/214 Sample & Assay Technologies 7
8 The AllPrep procedure AllPrep DNA/RNA/miRNA Universal Kit QIAcube Cells or tissue DNA mirna Sample Technologies On-column DNase digestion, including mirna rebinding Wash Total RNA For fatty tissue: chloroform extraction of flow-through Proteinase K digestion in the presence of ethanol Add ethanol Lyse and homogenize Bind genomic DNA Genomic DNA Wash On-column Proteinase K digestion RNA DNA RNA mirna AllPrep DNA/RNA/miRNA Universal Kit DNA mirna RNA DNA RNA/miRNA DNA RNA/miRNA Eluted RNA, including mirna Elute Bind total RNA Wash Elute AllPrep DNA/RNA/miRNA Universal Kit (5) , Eluted DNA Wash Deparaffinization solution FFPE Deparaffinization Solution DNA RNA Deparaffinization Solution FFPE Proteinase K Deparaffinization Solution FFPE Deparaffinization Solution EpiTect Plus Bisulfite Kit QIAamp DNA FFPE Tissue Kit RNeasy FFPE Kit mirneasy FFPE Kit QIAsymphony RNA Kit QIAsymphony DNA Mini Kit QIAsymphony DSP DNA Mini Kit Deparaffinization Solution (16 ml) ,5 Deparaffinization Solution (5 ml) , 8 QIAGEN Product News Issue 1/214
9 QIAamp Fast DNA Stool Mini Kit QIAcube QIAamp Fast DNA Stool Mini Kit gdna InhibitEX Buffer QIAcube PCR Sample QIAamp Fast DNA Stool Mini Kit DNA InhibitEX Buffer PCR Suspend in InhibitEx Buffer Sample Technologies PCR PCR QIAamp Fast DNA Stool Mini Kit InhibitEX Buffer PCR DNA C T value Standard column purification QIAamp Fast DNA Stool Mini Kit Fully automatable on the QIAcube Digest proteins Bind Wash 2x Sample Elute PCR QIAamp Fast DNA Stool Mini Kit 6 DNA C T PCR PCR QIAamp Fast DNA Stool Mini Kit DNA DNA ready for downstream applications QIAamp Fast DNA Stool Mini Kit (5) , 23, QIAGEN Product News Issue 1/214 Sample & Assay Technologies 9
10 Assay Technologies Sample collection Purification cdna synthesis Amplification Analysis Assay Technologies C T Detection frequency (%) Calculated Experimental 1 1 Copy number 3 ng DNA 3 ng DNA.3 ng DNA.3 ng DNA 3 pg DNA Copy number A QuantiNova Probe PCR Kit DNA CFTR ng gdna C T pg gdna 6 B QuantiNova Probe PCR Kit PCR 1 1 duplex 3 1 QuantiNova Probe PCR Kit PCR PCR PCR cdna gdna singleplex duplex 1 DNA QuantiNova Probe PCR Kit 1 QuantiNova Probe PCR Kit passive reference ROX ROX QuantiNova Probe PCR Kit QuantiNova Probe PCR Kit (1) , QuantiNova Probe PCR Kit (5) ,5 QuantiNova Probe PCR Kit (25) , 1 QIAGEN Product News Issue 1/214
11 QuantiNova SYBR Green PCR Kit SYBR Green qpcr 1 QuantiTect Primer Assay QuantiNova SYBR Green PCR Kit SYBR Green qpcr PCR PCR 1 cdna gdna C T value ng 3 ng.3 ng.3 ng 3 pg Copy number Detection frequency (%) 8 6 Calculated Experimental Assay Technologies 1 QuantiNova SYBR Green PCR Kit 1 DNA QuantiNova SYBR Green PCR Kit SYBR Green PCR QuantiNova SYBR Green PCR Kit QIAGEN QuantiNova QuantiNova QuantiNova SYBR Green PCR Kit PCR Copy number 1 A QuantiNova SYBR Green PCR Kit ng DNA IL1R2 C T 1 1, C T B QuantiNova QuantiNova SYBR Green PCR Kit (1) ,5 QuantiNova SYBR Green PCR Kit (5) , QuantiNova SYBR Green PCR Kit (25) , QIAGEN Product News Issue 1/214 Sample & Assay Technologies 11
12 Assay Technologies ~2 DNA samples from different populations Type-it CNV Reference Assay Type-it CNV Reference Assay 192 CNV qpcr 18 CNV A B CNV 1 C T GOI GOI 1 1 GOI GOI CNV Type-it CNV Probe PCR +qc Kit Type-it CNV SYBR Green PCR Kit CNV Copy Number Variation CNV CNV CNV QIAGEN Type-it Type-it CNV Type-it CNV PCR +qc Kits SYBR Green Probe A Calculated GOI copy number B CNV reference Type-it CNV Reference Assay Single-copy gene False loss False gain No change +1 copy 1 copy Copy number of CNV quantification reference Copy number, per diploid human genome C T change (reference assay) GOI copy number (real) 18-copy element (Type-it CNV Reference) Single-copy gene; not changed Single-copy gene; copy number +/ 1 GOI copy number (calculated) CNV calling for GOI NC: Correct NC: Correct NC: Correct NC: Correct Loss: False Gain: False Type-it CNV Probe PCR +qc Kit (1) ,5 Type-it CNV Probe PCR +qc Kit (4) , Type-it CNV SYBR Green PCR +qc Kit (1) ,5 Type-it CNV SYBR Green PCR +qc Kit (4) ,5 Type-it CNV SYBR Green PCR Core Kit (4) , QIAGEN Product News Issue 1/214
13 Type-it HRM PCR Kit Rotor-Gene Type-it HRM Discovery Kit HRM High Resolution Melt SNP PCR EvaGreen HRM QIAGEN PCR キット プロトコールに準拠 時間 至適化なしで PCR 解析成功 実験系の構築 時間 PCR 条件の至適化 時間 煩雑な至適化後のみ PCR 解析が可能 Type-it HRM PCR Kit HRM HRM PCR Rotor-Gene Q Rotor-Gene 6 LightCycler 48 Applied Biosystems 75 Fast System HRM PCR Rotor-Gene Type-it HRM Discovery Kit Rotor-Gene Q HRM PCR QIAGEN PCR QIAGEN PCR PCR Normalized fluorescence Assay Technologies Type-it HRM PCR Kit (1) , Type-it HRM PCR Kit (4) , Type-it HRM PCR Kit (2) , Rotor-Gene Type-it HRM Discovery Kit ,5 A/T Class IV SNP Type-it HRM PCR Kit GYS1 SNP rs227938type-it HRM PCR Kit Normalized melting curve Class IV SNP 3 Rotor-Gene Q PCR PCR Rotor-Gene Q 1/2 ±.2 Rotor-Gene Q HRM QIAGEN Product News Issue 1/214 Sample & Assay Technologies 13
14 Epigenetics Sample collection Purification & bisulfite conversion DNA Amplification Analysis EpiTect Fast Bisulfite Conversion Kits DNA FFPE 3 Bisulfite Epigenetics EpiTect Fast LyseAll Bisulfite Kit Whole blood Cultured cells or tissues DNA Bisulfite converted DNA FFPE tissues EpiTect Fast DNA Bisulfite Kit EpiTect Fast Bisulfite Kit DNA Bisulfite DNA DNA Bisulfite [FU] EpiTect Fast Supplier Z Supplier E [nt] EpiTect Fast FFPE Bisulfite Kit 99 3 Bisulfite gdnaffpe DNA DNA DNA Protect EpiTect Fast Bisulfite Conversion Kit 3 Bisulfite DNA Bisulfite EpiTect Fast Bisulfite Conversion Kits Bisulfite Solution 2 X 1 X DNA Bisulfite DNA Bisulfite DNA DNA DNA QIAGEN DNA Protect Buffer DNA DNA DNA Bisulfite DNA EpiTect Fast DNA Bisulfite Kit Bisulfite 1 µg DNA bp 6 kb 5 bp 1 kb Agilent RNA 6 Nano Chip 14 QIAGEN Product News Issue 1/214
15 EpiTect Fast DNA Bisulfite Kit EpiTect Fast 96 Bisulfite Kit QIAcube * EpiTect Fast DNA Bisulfite Kit DNA Bisulfite Bisulfite Solution 3 Bisulfite DNA Protect QIAGEN DNA EpiTect Fast DNA Bisulfite Kit 2 µl 1 ng 2 µg DNA 4 µl 1 5 ng DNA * EpiTect Fast FFPE Bisulfite Kit EpiTect Fast 96 FFPE Bisulfite Kit formalin-fixed paraffin-embedded FFPE EpiTect Fast FFPE Bisulfite Kit FFPE EpiTect Fast FFPE Lysis Kit DNA Bisulfite EpiTect Fast DNA Bisulfite Kit Bisulfite FFPE 1 µm QIAGEN DNA Protect Bisulfite DNA EpiTect Fast LyseAll Bisulfite Kit EpiTect Fast 96 LyseAll Bisulfite Kit EpiTect Fast LyseAll Bisulfite Kit EpiTect Fast LyseAll Lysis Kit DNA Bisulfite EpiTect Fast DNA Bisulfite Kit DNA DNA.5 2 µl pg DNA Epigenetics DNA Bisulfite EpiTect Fast Bisulfite Kit (1) ,5 EpiTect Fast DNA Bisulfite Kit (5) ,5 EpiTect Fast DNA Bisulfite Kit (2) , EpiTect Fast 96 Bisulfite Kit , FFPE Bisulfite EpiTect Fast FFPE Bisulfite Kit (5) ,5 EpiTect Fast FFPE Bisulfite Kit (2) , EpiTect Fast 96 FFPE Bisulfite Kit , Bisulfite EpiTect Fast LyseAll Bisulfite Kit (5) , EpiTect Fast LyseAll Bisulfite Kit (2) , EpiTect Fast 96 LyseAll Bisulfite Kit , QIAGEN Product News Issue 1/214 Sample & Assay Technologies 15
16 A B ESR1 A GeneGlobe PyroMark CpG Assay CpG CpG B ESR1 CpG 2 PyroMark CpG Assays DNA CpG GeneGlobe PyroMark CpG Assay CpG PyroMark CpG Assay 84, PyroMark CpG Assay (2) Varies 18, PyroMark CpG Assay 96 well (5) Varies 11,5 Epigenetics PyroMark CpG Assay 96 well (2) Varies 18, PyroMark Q24 Advanced Reagents Advanced CpG CpN DNA PyroMark Q24 Advanced DNA PyroMarkQ24 24 DNA PyroMark Q24 Advanced Reagents DNA PyroMark Q24 Advanced Reagents PyroMark Q24 Advanced Systems dntp PyroMark Q24 Advanced CpG Reagents PyroMark Q24 Advanced Reagents (4 x 24) ,5 PyroMark Q24 Advanced CpG Reagents (4 x 24) , 16 QIAGEN Product News Issue 1/214
17 Microbiome Sample collection Sample disruption Purification qpcr and qrt-pcr Analysis Microbial DNA qpcr Arrays PCR Microbial DNA qpcr Array 16s rrna DNA DNA DNA PCR Microbial qpcr Mastermix PCR Microbial DNA qpcr Arrays Varies Varies Sample A B C D E F G 73 H Host1 Host2 PanA/C PanB1 PanB2 PPC Sample Microbial DNA qpcr Array DNA pan-bacterial DNA pan-fungal DNA PCR 1. Microbial DNA qpcr Arrays * Antibiotic Resistance Genes (191) Food Testing: Poultry (111) Sepsis (193) Microbiome Bacterial Vaginosis (141) Food Testing: Seafood (112) Urinary Tract Infections (125) Custom Microbial DNA qpcr Arrays PCR Microbial DNA qpcr Assay Biodefense (121) Food Testing: Dairy (142) Food Testing: Meat (122) Food Testing: Vegetable (113) Intestinal Infections (143) Respiratory Infections (144) Vaginal Flora (192) Water Analysis (155) Custom user-selected assay panels * Custom Microbial DNA qpcr Array Custom Microbial DNA qpcr Array Inquire QIAGEN Product News Issue 1/214 Sample & Assay Technologies 17
18 C T LOD LLOQ y = x R² = Log target copy number vs LOD LLOQ LOD 95 LLOQ LOD 4 Microbial DNA qpcr Assays LLOQ Microbial DNA qpcr Assays Microbial DNA qpcr Assay Kits PCR qpcr Microbial DNA qpcr Assay PCR 2 PCR 1 µm 5'- 5 µm Microbial DNA qpcr Assay PCR Microbial qpcr Mastermixes Microbial DNA-Free Water Microbial DNA qpcr Assay Kits Microbial DNA Positive Control Positive PCR Control Microbial DNA-Free Water Microbial qpcr Mastermix Microbiome ΔC T ([metagenomic sample + synthetic template] synthetic template) Assay Micorbial DNA qpcr Assays Microbial DNA qpcr Assays C T PCR C T 35 C T C T C T C T 3 Microbial DNA qpcr Assays Microbial DNA qpcr Assays ,5 Microbial DNA qpcr Assay Kit , Microbial DNA qpcr Multi-Assay Kits PCR qpcr Microbial DNA qpcr Assay 5' Microbial DNA qpcr Multi-Assay Kits PCR PCR 2 PCR 1 µm 1 5' 5 µm Microbial DNA qpcr Assay qpcr Microbial DNA Positive Control Positive PCR Control Microbial DNA-Free Water Microbial qpcr Mastermix Microbial DNA qpcr Multi-Assay Kits Varies Varies 18 QIAGEN Issue 1/214
19 Supplemental Microbial qpcr Mastermix (Fluorescein) Supplemental Microbial qpcr Mastermix (ROX) Microbial DNA qpcr Assays Supplemental Microbial qpcr Mastermix ROX Fluorescein 5' PCR supplemental mastermix Microbial DNA qpcr Array Assay Kit Microbial qpcr Mastermix PCR DNA Taq Fluorescein ROX 5' Microbial DNA qpcr Assays Supplemental Microbial qpcr Mastermix Microbial DNA Positive Control Microbial DNA qpcr Assays Microbial DNA Positive Control Microbial DNA qpcr Assay Microbial DNA qpcr Assay DNA Microbial DNA Positive Control 1 Microbial DNA-Free Water Microbial DNA-Free Water Microbial DNA qpcr Microbial DNA-Free Water DNA UV DNA Microbial DNA qpcr Assay PCR Microbial DNA-Free Water PCR Microbial DNA qpcr Assay Array Microbiome Supplemental Microbial qpcr Mastermix (Fluorescein) , Supplemental Microbial qpcr Mastermix (ROX) , Microbial DNA Positive Control ,8 Microbial DNA-Free Water ,7 QIAGEN Product News Issue 1/214 Sample & Assay Technologies 19
20 Food Safety Testing pre-treatment Sample preparaiton Assay setup Detection mericon Sample Preparation Kits DNA CTAB DNA DNA PCR mericon Sample Preparation Kits mericon DNA Bacteria Bacteria Plus KitsDNeasy mericon Food Kit PCR DNA mericon PCR Assay Kits PCR GMO DNA mericon DNA Bacteria Kit DNA DNA DNeasy mericon 96 QIAcube HT Kit DNA mericon DNA Bacteria Plus Kit DNA DNeasy mericon Food Kit DNA Food Safety Testing mericon PCR Assays PCR 2 mericon mericon PCR mericon PCR n n n DNA DNeasy mericon Food Kit (5) QIAcube ,5 mericon DNA Bacteria Kit (1) , mericon DNA Bacteria Plus Kit (5) , DNeasy mericon 96 QIAcube HT Kit (5) QIAcube HT , 2 QIAGEN Product News Issue 1/214
21 Animal & veterinary Testing pre-treatment Sample preparaiton Assay setup Detection QIAamp cador Pathogen Mini Kit QIAcube RNA/DNA DNA RNA/DNA DNA RNA PCR/RT-PCR QIAamp cador Pathogen Mini Kit RNA/DNA DNA PCR RT-PCR C T Bovine blood 1 Bovine blood 2 Ovine blood BVDV QIAamp cador Pathogen Mini Kit cador Pathogen 96 QIAcube HT Kit Ovine serum Bovine serum Kidney lysate Liver lysate cador Pathogen 96 QIAcube HT Kit RNA/DNA DNA 1 RNA DNA DNA QIAcube HT PCR/RT-PCR cador Pathogen 96 QIAcube HT Kit QIAcube HT RNA DNA DNA 96 QIAamp QIAamp cador Pathogen Mini Kit (5) , QIAamp cador Pathogen Mini Kit (25) , cador Pathogen 96 QIAcube HT Kit (5) , C T Bovine blood 1 Bovine blood 2 Ovine blood Salmonella Ovine serum Bovine serum n RNA virotype n DNA cador bactotype QIAamp cador Pathogen Mini Kit cador Pathogen 96 QIAcube HT Kit Kidney lysate Liver lysate RNA DNA BVDV Salmonella QIAcube HT cador Pathogen 96 QIAcube HT Kit QIAamp cador Pathogen Mini Kit RNA DNA A BVDV B Salmonella C T 3 replicates ± 1 SD Animal & veterinary Testing QIAGEN Product News Issue 1/214 Sample & Assay Technologies 21
22 Human Identity & Forensic Testing pre-treatment Sample preparaiton Assay setup Quantification STR analysis QIAGEN Quality Management System QIAGEN Quality Management System QMS QMS ISO 91 ISO CMDCAS ISO FDA 21CFR Part MPG BGB1IS.3146 IVDD 98/79 EC MDD 93/42/EEC Technical Cooperation Program TCP Certified Management System EN ISO 91 EN ISO Investigator QIAGEN Investigator Kit EO DNA CoA Investigator Kit ISO QIAGEN Human Identity & Forensic Testing QIAGEN forensic grade QIAGEN Forensic Grade 22 QIAGEN Product News Issue 1/214
23 Investigator Lyse&Spin Basket Kit µm Investigator Lyse&Spin Basket Kit EZ1 DNA Investigator Kit QIAsymphony DNA Investigator Kit QIAamp DNA Investigator Kit Sample Spin basket 2ml tube 1 ATL/PK lysis buffer Lysis Lysate separation Lyse&Spin Basket 2 ml 2 Lysate Investigator Lyse&Spin Basket Kit (5) , 2 ml tube 2 QIAamp DNA Investigator Kit DNA DNA QIAcube QIAamp DNA Investigator Kit DNA QIAamp DNA Investigator Kit (5) ,5 12 D3S1358 vwa D16S539 D2S D8S117 D21S11 D18S TH1 12 D19S43 8 FGA DNA STR QIAamp DNA Investigator Kit DNA GeneMapper ID Software v3.2.1 Applied Biosystems STR Human Identity & Forensic Testing QIAGEN Product News Issue 1/214 Sample & Assay Technologies 23
24 DNA DNA QIAamp DNA Blood Kits QIAamp DNA Mini Kit QIAamp DNA Micro Kit QIAamp DNA Stool Mini Kit QIAamp DNA Investigator Kit QIAamp MinElute Media Kit QIAamp Fast DNA Stool Mini Kit QIAamp DNA FFPE Tissue Kit QIAamp Circulating Nucleic Acid Kit QIAamp UCP Pathogen Mini Kit * n n n n n n n n DNA DNA n n n n n n n n CSF DNA n n n n n n n n n n n n * n n n n n n n n LMD * n n n n n n n n n n n n n n n n QIAamp UCP PurePathogen Blood Kit MagAttract HMW DNA Kit Gentra Puregene Blood Kit Gentra Puregene Buccal Cell Kit QIAamp MinElute Virus Kits QIAamp UltraSens Virus Kit PAXgene Blood DNA Kit PAXgene Tissue DNA Kit n * DNA DNA RNA RNA PAXgene Tissue Container 24 QIAGEN Product News Issue 1/214
25 DNA DNA DNeasy Blood & Tissue Kit DNeasy Plant Mini and Maxi Kits QIAamp DNA Stool Mini Kit MagAttract HMW DNA Kit QIAamp Fast DNA Stool Mini Kit QIAamp DNA FFPE Tissue Kit Gentra Puregene Cell Kit Gentra Puregene Buccal Cell Kit Gentra Puregene Tissue Kit Gentra Puregene Yeast/Bact. Kit Gentra Puregene Mouse Tail Kit mericon DNA Bacteria Kit DNeasy mericon Food Kit n n n n n* n n* n* n* n n n n n n n n n n n n n n n n n n mericon DNA Bacteria Plus Kit 96 n n n n n n n n n n n n n n n n n DNeasy 96 Blood & Tissue Kit DNeasy 96 Plant Kit n * QIAGEN AllPrep DNA/RNA Kit DNA RNA AllPrep DNA/RNA/Protein Mini Kit QIAGEN Product News Issue 1/214 Sample & Assay Technologies 25
26 RNA RNA RNeasy Kits* RNeasy Fibrous Tissue Kits RNeasy Plus Universal Kits RNeasy Plus Micro Kit RNeasy FFPE Kit n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n n 5 mg n n n FFPE n PAXgene Bone Marrow RNA System RNAlater RNA Stabilization Reagent Allprotect Tissue Reagent DNA RNA FFPE TissueRuptor TissueLyser II n n * RNeasy Protect Kits RNeasy Plus Kits AllPrep Kits EZ1 RNA Tissue Mini Kit QIAsymphony RNA Kit RNeasy 96 Universal Tissue Kits mirneasy Kits PAXgene Tissue RNA Kit PAXgene Tissue mirna Kit EZ1 RNA Universal Tissue Kit mirna mirneasy FFPE Kit 26 QIAGEN Product News Issue 1/214
27 RNA RNA RNA DNA RNA RNA RNA RNA n* RNA RNA mirna RNA mirna DNA RNeasy Protect Cell Mini Kit PAXgene Blood RNA System RNeasy Protect Kits QIAcube RNeasy Protect Bacteria Kits n Allprotect Tissue Reagent RNAlater TissueProtect Tubes PAXgene Bone Marrow RNA System PAXgene Tissue System n n n n n RNAprotect Bacteria Reagent RNeasy Protect Saliva Mini Kit RNAlater RNA Stabilization Reagent RNAprotect Cell Reagent n n n n n n RNeasy Protect Animal Blood System Allprotect Tissue Reagent RNAlater RNA Stabilization Reagent n * QIAcube BioRobot Universal System QIAsymphony SP DNA QIAGEN Trademarks and Disclaimers QIAGEN Handbook User Manual Trademarks: QIAGEN, QIAcube, QIAamp, QIAsymphony, QuantiTect, AllPrep, BioRobot, cador, PDNeasy, Gentra, GeneGlobe, EpiTect, MagAttract, MinElute, mericon, Puregene, PyroMark, REPLI-g, RNAprotect, RNeasy, Rotor-Gene, Type-it, InhibitEX (QIAGEN Group); Agilent (Agilent Technologies, Inc.); Illumina, MiSeq (Illumina, Inc.); Applied Biosystems, ROX, GeneMapper, LightCycler, SYBR (Life Technologies, Inc.); PAXgene (PreAnalytiX GmbH); Excel, Microsoft (Microsoft Corporation). QR /QR Code RNAlater is a trademark of AMBION, Inc., Austin, Texas and is covered by various U.S. and foreign patents. 214 QIAGEN, all rights reserved QIAGEN Product News Issue 1/214 Sample & Assay Technologies 27
28 n n n Forefront Tower II Tel: n Fax: n techservice-jp@qiagen.com /214 Sample & Assay Technologies
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PCR 3 Mature mirna PCR 7 miscript mirna PCR Array C T 12 Mature mirna cdna 15 miscript mirna QC PCR Array miscript mirna PCR Array 10/2011
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PAXgene Tissue DNA Kitプロトコールとトラブルシューティング(2301605 06/2009)
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