2015 Nov % 7 00 ~ h 3 d I /R mg /kg h 10% 400 mg /kg Longa MCAO 4 10 mg /kg 2 h 0. 2 ml /100 g 6 h 23 ~ 25
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1 1208 CN /R ISSN http / /www. cjcpt. com 2015 Nov & SD 50 1 mg /kg 3 mg /kg mg /kg middle cerebral artery occlusion MCAO 2 h 2 h 6 h 6 h HE CA1 enzyme linked immunosorbent assay ELISA 3 neuronal specific enolase NSE S100-β neuron-specific enolase NSE S100-β NSE S100-β P < P < S100-β R A WS0119 M5 Tel sdyx91@ 126. com Tel dugh@ imm. ac. cn Sigma A S100-βELISA NSE ELISA EOS450D Molecular Devices CA USA spectramax 1. 2 SPF SD ~ 300 g SCXK
2 2015 Nov % 7 00 ~ h 3 d I /R mg /kg h 10% 400 mg /kg Longa MCAO 4 10 mg /kg 2 h 0. 2 ml /100 g 6 h 23 ~ h 6 h 10 mg /kg h HE 200 A B C 10 mg /kg 2 4% PBS 2 h 6 h 4% 48 h 5 μm HE 10 mg /kg CA NSE S100-β r /min 10 min S100-β ELISA NSE ELISA NSE S100-β 1. 5 ± x ± s ANOVA Student-Newman-Keuls post hoc P < h HE HE 1 A B C 10 mg /kg
3 1210 Chin J Clin Pharmacol Ther 2015 Nov CA1 NSE P < P < 3 CA mg /kg S100-β P < P < h CA1 10 mg /kg CA NSE S100-β h CA1 NSE S100-β P < mg /kg 200 A B C 10 mg /kg 1 6 h NSE S100-β x ± s n = 10 NSE ng /ml S100-β pg /ml 1 mg /kg 3 mg /kg 10 mg /kg ± ± c ± ± e ± f ± ± c ± e ± e ± f c P < e P < f P < CA1 5 NSE α- αα ββ γγ αβ αγ 5 γγ NSE NSE 7 NSE NSE S100-β 6 h 24 h
4 2015 Nov S100-β imaging J. Stroke Gao M Liu R Zhu SY et al. Acute neurovascular u- nit protective action of pinocembrin against permanent S100-β cerebral ischemia in rats J. J Asian Nat Prod Res S100-β / Gao M Zhu SY Tan CB et al. Pinocembrin protects NSE S100-β the neurovascular unit by reducing inflammation and 19 extracellular proteolysis in MCAO rats J. J Asian 2 h 6 h Nat Prod Res Wang SB Pang XB Gao M et al. Pinocembrin protects rats against cerebral ischemic damage through sol- NSE S100-β uble epoxide hydrolase and epoxyeicosatrienoic acids 2 h J. Chin J Nat Med h S100-β 11 Marangos PJ Schmechel DE. Neuron specific enolase a clinically useful marker for neurons and neuro- NSE endocrine cells J. Annu Rev Neurosci Marquardt G Setzer M Szelenyi A et al. Prognostic relevance of serial S100b and NSE serum measurements in patients with spinal intradural lesions J. Neurol Res Gazzolo D Abella R Marinoni E et al. Circulating biochemical markers of brain damage in infants complicated by ischemia reperfusion injury J. Cardiovasc Hematol Agents Med Chem Zhao G Zhang W Li L et al. Pinocembrin protects the brain against ischemia-reperfusion injury and reverses the autophagy dysfunction in the penumbra area J. Molecules Meng F Wang Y Liu R et al. Pinocembrin alleviates memory impairment in transient global cerebral ischemic rats J. Exp Ther Med Wu CX Liu R Gao M et al. Pinocembrin protects brain against ischemia / reperfusion injury by attenuating endoplasmic reticulum stress induced apoptosis J. Neurosci Lett Longa EZ Weinstein PR Carlson S et al. Reversible middle cerebral artery occlusion without craniectomy in rats J. Stroke Ⅱ MCAO IL-17 IL-6 J Pulsinelli WA. Selective neuronal vulnerability morphological and molecular characteristics J. Prog Brain Res Bardutzky J Shen Q Henninger N et al. Characterizing tissue fate after transient cerebral ischemia of varying duration using quantitative diffusion and perfusion 14 Barone FC Clark RK Price WJ et al. Neuron-specific enolase increases in cerebral and systemic circulation following focal ischemia J. Brain Res Oksanen T Tiainen M Skrifvars MB et al. Predictive power of serum NSE and OHCA score regarding 6- month neurologic outcome after out-of-hospital ventricular fibrillation and therapeutic hypothermia J. Resuscitation Vinores SA Herman MM Rubinstein LJ et al. Electron microscopic localization of neuron-specific enolase in rat and mouse brain J. J Histochem Cytochem Florio P Abella R Marinoni E et al. Biochemical markers of perinatal brain damage J. Front Biosci Schol Ed NSE J Kaca-Orynska M Tomasiuk R Friedman A. Neuronspecific enolase and S 100B protein as predictors of outcome in ischaemic stroke J. Neurol Neurochir Pol
5 1220 Chin J Clin Pharmacol Ther 2015 Nov Experimental study on effect of galanthamine reducing inflammatory reaction in sepsis YANG Yi YANG Jin ICU of the Third People's Hospital of Chongqing City Chongqing China ABSTRACT AIM To study the effect of brain cholinesterase inhibitors galanthamine on anti-inflammatory response in sepsis. METHODS 50 Male SD rats were randomly divided into sham operated SO group sepsis SEP group galanthamine Gal group atropine Atr group galanthamine + atropine Gal + Atr group. Sepsis model was produced by cecal ligation and puncture CLP. After 6 hours IL-6 IL-1β and arterial blood lactic levels in blood were determined and the tissues of lung and liver were harvested for histopathological analysis. RESULTS Compared with SO group IL-6 IL-1β and lactic levels in blood were increased P < and there were obvious pathologic changes in the lung and liver tissues in SEP group. Compared with SEP group IL-6 IL-1β and lactic levels in blood were all increased P < and there were more pathologic changes in the lung and liver tissues in Atr group. Compared with SEP group IL-6 IL-1β and lactic levels in blood were all decreased P < and the lung and liver tissue pathologic changes were slighter in Gal group. Atropine would offset such effects. CONCLUSION Galantamine might reduce the inflammation of sepsis by the cholinergic anti-inflammatory pathway. KEY WORDS sepsis cholinergic anti-inflammatory pathway galanthamine 櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂櫂 1211 Pinocembrin prevented brain acute injury induced by focal cerebral ischemia-reperfusion WU Cai-xia 1 DU Guan-hua 2 1 Shandong Medical College Linyi Shandong China 2 National Center for Pharmaceutical Screening Institute of Materia Medica Chinese Academy of Medical Sciences Beijing China ABSTRACT AIM The aim of this work was to verify neuroprotective and properties of Pinocembrin then provide a reference for further analysis of its mechanism on ischemic stroke. METHODS The study was carried out on 50 male Sprague-Dawley rats weighing g which were divided into five groups i control n = 10 ii I /R n = 10 iii I /R + Pinocembrin 1 mg /kg n = 10 iv I /R + Pinocembrin 3 mg /kg n = 10 v I /R + Pinocembrin 10 mg /kg n = 10. Focal cerebral ischemia / reperfusion rats were induced by middle cerebral artery occlusion MCAO for 2 h followed by 6 h reperfusion. Pinocembrin was administered in doses 1 mg /kg 3 mg /kg 10 mg /kg respectively at the same time of onset of reperfusion. Then the blood NSE and S-100β were determined in addition to observe the pathological change of hippocampus penumbra cortex and corpus striatum respectively. RESULTS Pinocembrin-treatment 3 mg /kg or 10 mg /kg significantly suppressed the levels of blood NSE and S-100β and improved the morphology of brain cortex striatum and hippocampus of acute focal cerebral ischemia reperfusion 10 mg /kg. CONCLUSION Pinocembrin has neuroprotective effects to prevent brain ischemia / reperfusion acute injury. KEY WORDS Pinocembrin acute cerebral ischemia reperfusion injury NSE S100-β
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