Key words: Nocardia asteroides, Nocardiosis, Minocycline
Table 1. Relationship between date of detection in culture and number of colony of N. asteroides isolated from the patients in various media.
Table 2 Nocardial isolates of 8 patients. LLL= left lower lobe, BUL= bilateral upper lobe, LUL= left upper lobe
Table 3. Susceptibility of N. asteroides isolated from the patients.
Table 4. Nocardia asteroides infections reported in Japan since 1969.
84: 337-347, 1961. 9) Staneck, J. L., Roberts, G. D.: Simplified approach to identification of aerobic actinomy- cetes by thin-layer chromatography. Appl. Microbiol., 28: 226-231, 1974. 13) Berd, D.: Nocardia asteroides. A taxonomic study with clinical correlations. Am. Rev. Resp. Dis., 108: 909-917, 1973. 14) Tsukamura, M.: Numerical taxonomy of the genus Nocardia. J. Gen. Microbiol., 56: 265-287, 1969. 15) Kurup, P. V., Randhawa, H. S., Gupta, N. P.: Nocardiosis: a review. Mycopathologia et Mycologia applicata., 40: 193-219, 1970. 17) Bobbitt, O. B., Friedman, I. H. and Lupton, C.: Nocardiosis, report of three cases. New Eng. J. Med., 252: 893-897, 1955. 18) Williams, D. M., Krick, J.A. and Remington, J. S.: Pulmonary infection in the compromised host. Part I. Am. Rev. Resp. Dis., 114: 359-394, 1976. 19) Bach, M. C., Sabath, L. D., Finland, M.: Susceptibility of Nocardia asteroides to 45 3) Young, L. S., Armstrong, D., Blevins, A. and antimicrobial agents in vitro. Antimicrob. Ag. Chemoth., 3: 1-8, 1973. Lieberman, P.: Nocardia asteroides infection complicating neoplastic disease. Am. J. Med., 20) Lerner, P. I., and Baum, G. L.: Antimicrobial susceptibility of Nocardia species. Antimicrob. 50: 356-366, 1971. Ag. Chemoth., 4: 85-93, 1973. 4) Goodman, J. S. and Koenig, M. G.: Nocardia infections in a general hospital. Ann. New 21) Bach, M. C., Monaco, A. P. and Finland, M.: York Acad. Sci., 174: 552-567, 1970. Pulmonary nocardiosis. Therapy with minocycline and with erythromycin plus 5) Palmer, D. L., Harvey, R. L. and Wheeler, ampicillin. J. K.: Diagnostic and therapeutic considerations in Nocardia asteroides infection. Medicine, 53: 391-401, 1974. 6) Frazier, A. R., Rosenow, E. D. and Roberts, G. D.: Nocardiosis, a review of 25 cases occurring during 24 months. Mayo Clin. Proc., 50: 657-663, 1975. 7) Hasty, T. S., McDurmont, C., Ajello, L., et al.: Prevalence of nocardia asteriodes in sputa examined by a tuberculosis diagnostic laboratory. J. Lab. & Clin. Med., 58: 107-114, 1961. 8) Georg, L. K., Ajello, L., McDurmont, et al.: The identification of Nocardia asteroides and Nocardia brasiliensis. Am. Rev. Resp. Dis., J. A. M. A., 224: 1378-1381, 1973.
The Isolation of Nocardia asteroides and its Infection Cases Akio Kobayasi, Setuko Kubo and Masako Araki Department of Laboratory Medicine, Chiba University Hospital, Chiba, Japan N. asteroides was recovered from 8 patients during recent two-year period. In two patients who had been transplanted the kidneys, this microorganism was confirmed to be the pathogen causing the lesions of the lung and the chest wall. The both patients recovered by the treatment of minocycline with surgical drainage. On the other hand, the organism isolated was considered to be transient colonization in the other two patients. In the remaining 4 patients, N. asteroides isolated was suspected to be pathogenic. A patient with AML had N. asteroides repeatedly in her sputa, and the organism was eliminated rapidly by the treatment of minocycline, but she was superinfected by the other bacteria. Repeated isolations and reappearring of N. asteroides after discontinuation of the therapy were observed in a patient with bronchiectasis, but it never became manifested. The 2 patients having abnormal findings of chest roentogenogram had isolation of the organisms only once, but the follow up was failed. The colonies of N. asteroides mostly became recognizable within 3 days after inoculation of the specimens onto blood agar plates. Minimal inhibitory concentration of minocycline to the organisms isolated Erythromycin, ampicillin and trimethoprim-sulfomethoxazole had higher MIC compared with minocycline. The Japanese literatures describing 14 cases of N. asteroides infection since 1969 were reviewed.