SANRIKU SANRIKU KAUST

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... 3... 3... 6... 7 26... 7... 10 1.... 10... 10 SANRIKU 26... 21 SANRIKU 26... 24 KAUST 26... 27... 29 2.... 31... 37... 37... 38 2

2014 4 1 2011 3 PPA 2014 7 1964 50 1967 1972 Y 1 F1 1973 2 F2 3 F3 4 1974 F4 MB5 2 1 2009 2011 3 6,000 40 3

3 3 F4 MB5 1 2 F3 2014 3 F1 F2 2015 5 MB5 4 2014 King Abdullah University of Science and Technology KAUST 2 F4 2015 7 4

200 2 SANRIKU 1 1 1 SANRIKU 2014 8 2 1 F4 2015 8 5

1967 8 1972 4 1 F1 1973 3 2 F2 3 F3 4 1975 1 1978 9 1992 7 F4 2009 4 2009 3 MB5 23 2011 2011 3 2012 4 MB5 2F 2012 7 MB 2013 4 26 2014 2014 3 F3 2014 4 2014 7 KAUST MB5 4F 2014 8 2015 7 F1 F2 2015 7 2015 7 F4 MB5 SERC1 SERC2 6

26 23 2011 3 26 2014 4 A 4 B 6 2 1 1 26 26 2014 8 60 2 1 8 4 7 2 8 6 9 1 3 4 2 2 4 7

26 4 3 4 2 2 http://www.kitasato-u.ac.jp/mb/campus/movie.html 26 27 2015 1 1 27 26 27 27 26 8

1. 26 1. 26 9

1. 2011 3 11 ( ) 3TEAMS Tohoku Ecosystem-Associated Marine Science 10

4 http://www.i-teams.jp/j/about/index.html 47 40 2011 8 11 1. (1) (2) (3) 2. 3. 4. 5. http://www.kitasato-u.ac.jp/20110311/n20110811_54.html 2011 2012 11

3 1 2 3 4 5 1. ph COD DO DO a 2. 38 1 14 2 17 23 24 1 21 20 20 Perforatus perforatus 12

3. 2009 90 2014 5 16 29 1008 2013 2011 680 2014 8 3300 (n=6) DNA DNA 3 5. 2013 4 Alexandrium tamarense 2014 2012 2013 2012 1/10 2 2014 Alexandrium DA 13

4 2012 -- 44(11) 602-605 2012 2013/3/14 2013/3/14, 25 2013/3/27 24 2013/1/26 50 2012/11/16 24 2012/7/14 8 NCB 2013/3/9 8 NCB 2013/3/9., 75, 8-9. (2012) 2012 2012/10/7 14

2012/10/26 2013.3.14 2013/3/14 25 2013/3/27 2013/3/14 2012/10/26 6, 2013/3/14 2013/3/14 6 25 2013/3/29 4 N. navis-varingica 25 2013/3/29 2013. 3 3?., 2014.03.02.. 3 3? 15

., 2014.03.02. 2013 2013. 3 3?., 2014.03.02.. 3 3?., 2014.03.02. :, 26 2014.03.20, 26 3 2014.03.27 Balanus perforates, 2013 2013.03.30, MS 25 2014.06.08, 2, 2013,2013.10.28,,, 2013.11.29. 2013. 2013.9.21 16

.,, 2013.09.20.,, 2013.09.20. 3 3?., 2014.03.02.. 3 3?., 2014.03.02. 2013.06.08.. 3 3?., 2014.03.02.. 25 ( ),, 2013.06.08.. 26,, 2015.03.28. Nitzschia navis-varingica. 25,, 2013.09.21.. 3., 2014.03.02. 2014 17

Yuichiro Yamada, Shinnosuke Kaga, Yoshimasa Kaga, Kimiaki Naiki, Shiho Watanabe, Temporal and spatial variations in dissolved nutrients and chlorophyll a concentrations in Ofunato Bay, Iwate Prefecture: comparison of before and after the 2011 off the Pacific coast of Tohoku Earthquake. Journal of Oceanography ( ) 2014 2014.9.5 4 2015.3.1 a 4 2015.3.1 a 2014 2014.9.5 (2015). Perforatus perforatus PCR. Sessile Organisms, 32, 1-6, 2015 (2014).., 46, 72-77. 2015. 4 2015.3.1 2015.. 39,, 2015.3.21 2014. Perforatus perforatus. 2014.. 2014.09.05. Hamaguchi, H., Nogata, Y., Okano, K., Ogasawara, M., Tsuji, T., Nanba, N., Kado, R. A new alian barnacle Perforatus perforatus in northern Honshu, Japan Current distribution and its ecological properties, 2014 ICMCF Satellite Symposium in Singapore, 2014. 6 11. 4 2015.3.1 Perforatus perforatus.2015,, 2015.03.27. 18

2014.. 26 (2014/9/21) (2014). 26 (2014/11/8) 2014.. 26 2015/2/26 2015.. 26 2015/3/29 (2015).. 4 2015/3/1 2015. 4 2015/3/1 2014. TOMORROW. NHK BS 2014/8/20 ( ) 26 5 17 2014 3 1 2015. 26 ( ) 2014.5.17 :. 2015.3.1. 27 19

2015.3.29 26 2014.5.17. Romero MLJ, Relox J, Lundholm N, Yoshida K, Kobiyama A, Mashimo M, Ogawa H, Chen I-S, Haraguchi K, Kotaki Y Spatial distribution of ASP producing diatom Nitzschia navis-varingica along the warm current Kuroshio 26 2014.9.21 26 2014.9.21 Yuichi Kotaki, Nina Lundholm, Kenichi Yoshida, Atsushi Kobiyama, Masakazu Mashimo, Koichi Haraguchi: A survey on toxic diatoms Pseudo-nitzschia multiseries and Nitzschia navis-varingica after the 2011 Japanese tsunami, 16 th International Conference on Harmful Algae, Wellington, New Zealand, 2014. 10. 28. Marc Lawrence J. Romero, Yuichi Kotaki, Nina Lundholm, Atsushi Kobiyama, Juan Relox, Kenichi Yoshida, Masakazu Mashimo, I-Shiung Chen, Hisao Ogawa, Koichi Haraguchi: Geographical comparison in toxin profiles and genetic markers in the toxic diatom Nitzschia navis-varingica along the warm water current Kuroshio, 16 th International Conference on Harmful Algae, Wellington, New Zealand, 2014. 10.27. 2015.3.1. TEAMS: http://www.i-teams.jp/j/about/index.html http://www.kitasato-u.ac.jp/20110311/n20110811_54.html 20

SANRIKU 26 SANRIKU SANRIKU2011 3 24 SANRIKU 1 3 6 SANRIKU 21

1980 4 5 1990 1 2000 2010 2 22

SANRIKU 3 23

SANRIKU 26 23 2011 24 27 26 26 1 ( ) 24

ORAC CH 3 COOH H COOH COOH 2 N CH 3 H Alexandrium in vitro 25

26

KAUST 26 2009 King Abdullah University of Science and Technology ( KAUST) 2 100 KAUST KAUST 26 7 (2014.7) DNA 7.9km 2, 5.2km KSt1 KSt2 KSt3 3 26 7 2014.7 27 3 27

Md Shaheed Reza 15 2-2-3 26 3 1. KAUST 2 MB5 4 402 KAUST DNA 2. 26 8 KSt2 27 1 KSt1 KSt2 KSt3 3 3. DNA DNA DNA MiSeq KAUST KAUST 28

20 MBI 25 Pseudococcomyxa sp. KJ KJ 29

MBI 3. MBI 30

2. 4 PSP 1980 PSP Ogata et al., 1982 1961 PSP Alexandrium tamarense PSP A. tamarense A. catenella 10m A. tamarense A. catenella A. tamarense 31

A. catenella Kodama 1982 2000 A. catenella A. tamarense Sekiguchi et al., 2001 A. tamarense A. tamarense A. catenella A. catenella PSP Proctor 1975 Gonyaulax catenella A. catenella 32

Alexandrium A. tamarense Ogata et al., 1987 Proctor A. catenella A. tamarense A. tamarense A. tamarense Ogata et al., 1996 33

PSP 5 A.tamarense :676,500 cells/l, 2012 Alexandrium tamarense A. catenella 2012 14 0 3cm A. tamarense / A. catenella A. tamarense type 2012 9 A. tamarense type 1,000 cells/g 62,000 cells/g Protoperidinium Gonyaulax A. tamarense type A. tamarense A. tamarense type A. tamarense 2013 2014 Alexandrium 800 cells/g PSP 2012 2015 A. tamarense Kamiyama et al., 2014 2012 10 2 70cm 20 cm Alexandrium 34

PSP PSP Alexandrium A. tamarense : Alexandrium tamarense..44. 321-327 (2012) Kamiyama T, Yamauchi H, Nagai S, Yamaguchi M (2014) Differences in abundance and distribution of Alexandrium cysts in Sendai Bay, northern Japan, before and after the tsunami caused by the Great East Japan Earthquake. J. Oceanogr. 70: 185-195 Kodama M, Fukuyo Y, Ogata T, Igarashi T, Kamiya H, Matsuura F (1982) Comparison of toxicities of Protogonyaulax cells of various sizes. Bull. Japan. Soc. Sci. Fish. 48: 567 571 Ogata T, Kodama M, Fukuyo Y, Inoue T, Kamiya H, Matsuura F, Sekiguchi K, Watanabe S (1982) The occurrence of Protogonyaulax spp. in Ofunato Bay, in association with the toxification of the scallop Patinopecten yessoensis. Bull. Japan. Sci. Soc. Fish. 48: 563 566 Ogata T, Ishimaru T, Kodama M (1987) Effect of water temperature and light intensity on growth rate and toxicity change in Protogonyaulax tamarensis. Mar. Biol. 95: 217 220 Ogata T, Koike K, Nomura S, Kodama M (1996) Utilization of organic substances for growth and toxin production by Alexandrium tamarense. In: Yasumoto T, Oshima Y, Fukuyo Y (eds) Harmful and 35

Toxic Alagal Blooms. Intergovernmental Oceanographic Commission of UNESCO, Paris, pp 343 346 Sekiguchi K, Ogata T, Kaga S, Yoshida M, Fukuyo Y, Kodama M (2001) Accumulation of paralytic shellfish toxins in the scallop Patinopecten yessoensis caused by the dinoflagellate Alexandrium catenella in Otsuchi Bay, Iwate Prefecture, northern Pacific coast of Japan. Fisheries Sci. 67: 1157 1162 36

1 54 6 6 4 3 2 1 1 4 450 m 2 30 4 10 1,050 m 2 2 KAUST 4 4 3 3 2 1 37

022-0101 160-4 5 10 15 6 10 15 38

39