423 昭 和60年6,月28日 Fig. Panel 4.3, are œ; A B as SO and after follows; (slow B 1 are Microphotographs processed preincubation FG twitch for at (fast twitch oxidative a ph 4.0. cross ATPase Panel glycolitic fiber), stained myosin C fiber), ; X; capillaries, section reaction: is processed FOG for (fast preincubation SDH twitch 3 Fiber rent Mean: Muscle fiber type composition (C) and fiber type area composition (A) in diaphragm, soleus, plantaris and extensor digitorum longus (EDL) muscles rat. diaphragm. after at reaction. a ph The oxidative symbols glycolitic fiber), C. Fig. Fig. 2 A CAの cross types sectional in 4 average different are means }standard area cross fiber muscles sectional fiber types. diffe- rat. area Values deviations. 絶 対 値 は,各 筋 で1 10本 の範 囲 で あ っ た が, いず れ の筋 にお いて も筋 線 維 タイ プ の違 い に よ る差 は 認 め られ な か っ た(Fig.4).横 423 隔 膜 のCA値 は,ひ らめ

Table 1 Number muscle fibers and capillaries per 1 mm2 and capillaries to fiber (C/F) ratio in rat diaphragm, soleus, plantaris and extensor digitorum longus (EDL) muscles. Values are means } standard deviations Fig. 4 Number capillaries around each fiber type (CA) in 4 muscles rat. Values are means }standard deviations. Fig. 5 Number capillaries around each fiber type relative to fiber area (CA/area) in 4 muscles rat. Values are means }standard deviations.

Table 2 The different variables for each fiber type male and female rat's diaphragm. % fiber: fiber type composition, % area: fiber type area composition, CA: number capillaries around each fiber type, CA/area: number capillaries around each fiber type relative to fiber area 1000ƒÊm2 Table 3 Number muscle fibers and capillaries per 1 mm2 and capillaries to fiber (C/F) ratio in male and female rats. Values are means }standard deviations *: p<0.05 ***: p<0.001

Fig. 6 Changes fiber cross sectional area each fiber type (A), percentage different fiber types (B) and area percentage different fiber types and interstitum (inter, C) with increasing body weight in rat diaphragm, soleus and extensor digitorum longus (EDL) muscles. Values are means }standard deviations. Fig. 7 Changes muscle fiber density and capillary density per 1 mm2 (A), number capillaries around each fiber type (B) and number capillaries around each fiber type (CA) relative to fiber area 1000 Đm2 (C) with increasing body weight in rat three muscles. Values are means }standard deviations.

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kžó t 429 THE FIBER TYPE COMPOSITION AND THE CAPILLARITY OF THE RAT DIAPHRAGM Nobukazu TAMAKI Department Physiology, School Medicine, Showa University (Director: Pr. Santa ICHIKAWA) Abstract \ The fiber type composition and capillary supply in rat diaphragm were compared with those different hindlimb muscles. And n, effects sex difference and growth on se characteristics diaphragm were studied. Muscle samples obtained from anestized rat were rapidly frozen and sliced transversely in a cryostat. The sections were stained histochemically by succinate dehydrogenase method and myosin adenosine triphosphatase method after preincubation at ph 4.3 to typify fibers. The capillaries were stained by myosin adenosine triphosphatase method after preincubation at ph 4.0. The following results were obtained. The greater part rat diaphragm was composed fast twitch fibers, as well as fast muscle. The capillary supply in diaphragm was richer than that hindlimb muscles studied. For fiber type composition, re were no differences between male and female rats. The muscle capillarity in female rats were more richer than that male rat. The growth causes changing properties motoneurons and new capillary formation in diaphragm. Since diphragm was already adapted to a highly repetitive and low resistance type activity, it assumed that histochemical and morphological characteristics muscle fibers were related to functional requirements diaphragm. :3ŒŽ27 ú,1985 l