Aqua Way Philic Aqua Way Cation
Technology platform Cell-sheet engineering (1)
Technology platform Cell-sheet engineering (2) 1) Collection of cells in a form of thin film 2) o suture required for transplantation 3) Creation of 3D tissue by piling up cell-sheets
Technology platform Cell-sheet engineering (3)
Aqua Way Philic Aqua Way Cation
Poly(-isopropylacrylamide) ydration n polymer solution Dehydration 2 Lower Critical Solution Temperature (LCST) at 32 C 2 C C C 3 C C 3 n Poly(-isopropylacrylamide (PIPAAm)
Poly(-isopropylacrylamide):PIPAAm; LCST = 32C 2 Lower Critical Solution Temperature (LCST) 10C 37C /
Aqua Way Philic Aqua Way Cation
Aqua Way Philic
Column: AquaWay "Philic" 150 mm4.6 mm I.D. Mobile phasewater Flow rate1.0 ml/min DetectionUV 254 nm Sample hydrocortisone, testosterone 0 Uracil
Aqua Way Philic
40 Column: Aqua Way Philic 150mm4.6mm I.D. Mobile phase66.7mm phosphate buffer (p7.0) Flow rate1.0ml/min DetectionUV 220nm Temperature40 Sample Serum (20µl) 10
2 Carbamazepine
C 2 5 primidone
3 C C 3 theophylline
3 C 3 C C C Propofol (2,6-di-isopropylphenol) logp=3.579 C 3 C 3 38g/mL
Absorbance 0 10 20 30 1.05 µg/ml) Column : PIPAAm hydrogel modified Retention Time(min) Eluent: 2 UV:263nm (5µg/mL) Injection volume :10µL column(4.6mm 50mm) Flow-rate : 1.0mL/min Temp : 15
67 mm 1 ml/min 40 pump LC column 20l 10 UV 1 BCA ( micro BCA Protein Assay )
2 Column: Aqua Way Philic 150mm4.6mm I.D. Mobile phase 66.7mM phosphate buffer (p7.0) Flow rate1.0ml/min Temperature40 DetectionUV 220nm Samplecarbamazepine
1 2 CYPcytochrome P450 2 C 3 3 C phenacetin 1 olumn: Aqua Way Philic 150mm4.6mm I.D. obile phase66.7mm phosphate buffer (p7.0) low rate1.0ml/min etectionuv 220nm emperature40 ample 1 acetaminophen 2 phenacetin drug-metabolizing enzyme CYP1A2 3 C acetaminophen metabolism
1 2 3 4 1 C 3 2 3 C 3 C C 3 C 3 C C 3 anhydrous caffeine ibuprofen 3 4 3 C CCCC 2 3 C Br bromvarerylurea 2 C 3 ethenzamide Column: AquaWay "Philic" 150mm4.6mm I.D. Mobile phase 66.7mM phosphate buffer (p7.0) Flow rate1.0ml/min DetectionUV 230nm Temperature40 Sample 1 anhydrous caffeine 2 ibuprofen 3 bromovalerylurea 4 ethenzamide
1 2+3 4 5 1 C 3 4 C 3 C 3 C 3 C 3 1 2 3 4 5 hydrocortisone logp 1.61 5 C 3 C 3 prednisolone acetate logp 2.40 testosterone logp 3.32 Column: AquaWay "Philic" 2/min 150 mm4.6 mm I.D. 40 Mobile phase water Flow rate1.0 ml/min DetectionUV 254 nm Sample 1 hydrocortisone 10 2 cortisone 3 prednisolone 4 prednisolone acetate 5 testosterone
1,2 3 PLC conditions Detection: UV (210 nm) Eluent: 0.9 w/v% acl solution Flow rate: 1.0 ml/min 10 1 2 3 40 0 10 20 Retention time (min) 1.Insulin chain A 79.97 µg/ml 2.β-Endorphin fragment 1-27 (60.44 µg/ml) 3.Insulin chain B 859.8 µg/ml 0 10 20 Retention time (min) Kanazawa, et al. Anal. Bioanal. Chem. 378, 46 (2004)
37
Aqua Way Philic Aqua Way Cation
Poly(IPAAm-co-BMA-co-DMAPAA) IPAAm/BMA=95/5, DMAPAA 7.5 mol% 9.5 LCST of terpolymer in distilled water + 9.0 pka 8.5 pk a.. 8.0 /. 7.5 7.0 10 20 30 40 50 60 Temperature ( C) Kikuchi, kano
Aqua Way Cation
Aqua Way Cation
-P -2C AMP 2 2 -P--P-2C ADP 2 -P--P--P-2C ATP Table Some properties of solutes M.W. pka log P* AMP ADP ATP 347.2 3.80-3.52 427.2 3.90-4.00 551.1 4.06-4.60 * P ; Partition coefficient of n-octanol/water system. Kikuchi, kano
ATP PIPAAm Kikuchi, kano
ADP / ADP 2 2 P P ADP P 2 P ADP + P 2 P
ADP + +ADP ADP ADP
10, 40 UV 97.2 (kda) 66.4 45.0 2.0 0.3 0.8 1.2 0.9 0.2 2.0 (0.3) Western blotting albumi microbca
+ + + + + + + + + + + + + + + + + + + 10 40
Aqua Way Cation Aqua Way Philic
Aqua Way Aqua Way Philic Aqua Way Cation
Aqua Way Aqua Way Philic vs DS Aqua Way Cation vs
Aqua Way ydrophobicity neutral ydrophilicity
Aqua Way