pp. Na H Na H ph Na H NHE B CHP NHE NHE CHP NMR NHE NHE pcold-gst ph Na Na H Na H Na H Na H antiporter Nha Na H Na H Na H exchanger NHE C C C The regulation mechanism of Na H exchanger Kokoro Hayashi and Chojiro Kojima Laboratory of Biophysics, Graduate School of Biological Sciences, Nara Institute of Science and Technology NAIST, Takayama, Ikoma, Nara, Japan B calcineurin B homologous protein, CHPNHE NHE C CHP NHE NHE C CHP NMR NHE C CHP CHP NHE NHE Na H NHE NHE Na H Na NHE NHE NHE NHE NHE NHE NHE NHE NHE NHE NHE NHE NHE
NHE NHE NHE NHE NHE NHE NHE NHE NHE NHE ph phi Na NHE a phi phi NHE NHE ph NHE ph NHE NHE C C Na H NhaA NHE NHE a NHE b NHE PIP CHP B ERM CaM - - ROCK I Rho I NIK Nck CaII II.
NHE C NHE C C NHE C b NHE C Rho I ROCK I C PKC Nck NIK CaM CHP ERM PIP NHE CHP CHP NHE NHE phi NHE CHP CHP ph B CHP CHP CHP CHP CHP N GlyCHP CHP CHP CHPCHP NHE CHP CHP NHE ph ph ph ph CHP CHP CHP α B NHE C CHP NMR CHP NHE NHE CHP EF CHP EF N EF EF C EF EF CHP EF EF CHP NHE CHP NHEnM NHE CHP CHP N N C C CHP N C CHP B CHP CHP CHP unique region CHP NHE NHE phi CHP NHE NMR CHP NHE NMR CHP NHE NHE pcold-gst NHE NMR CHP NMR NMR CHP NHEkDa NMR PDB NMR kda NMR NMR CHP NHE C N NMR H H C N NOESY CYANA nuclear Overhauser effect NOE CANDID
９ ２ ８ 生化学 第８ ０巻 第１ ０号 図２ CHP NHE１ペプチド複合体の 立体構造 CHP の N-ローブを青色 C-ローブ をピンク色 CHP-ループを灰色 NHE１ペプチドを緑色で示す NHE ペプチドは ５ １ ６ ５ ４ ０ の領域を表 示 し た 左 CHP１ NHE１ペ プ チ ドの溶液構造 PDB ID ２E３ ０ 右 CHP２ NHE１ペ プ チ ド の 結 晶 構造 PDB ID ２BEC CHP-ルー プの点線は構造が決定されていない 領域 図３ CHP１と CHP２の表面電荷マップ CHP NHE１複合体における CHP の表面電荷を示す 正電荷は青 負電荷は赤 緑色のリボン構造は NHE１ペプチドを示す 黄色い点線は CHP１と CHP２で電荷が反転している領域 a CHP１ NHE１ペ プチド複合体 b CHP２ NHE１ペプチド複合体
NMR NOE Güntert kda NMR NMR CHP NHE residual dipolar coupling, RDC NMR RDC NH NH RDC CHP NHE NMR CHP NHE NMR CHP NHE CHP NHE NMR CHP NHE X CHP NHE CHP NHE CHP NHECHP CHP NHE CHP NHE NHECHP NHE NHE α CHP CHP C NHE N CHP N NHE C NHE CHP CHP CHP NHE C N CHP CHP CHP NHENHE C CHP CHP NHE CHP NHE NHE α C C O CHP α NH CHP NHE NHE CHP CHP CHP CHP CHP NHE CHP NHE ph NHE NhaA ph NHE IL b RCHP CHP phi CHP CHP NHE IL CHP NHE ph CHP NHE b NHE CHP NHE CHP NHE pcold-gst NHE pcold-gst NHE C S- GST pcold I pcold I N His cspa lac operator a IPTG
pcold a pcold I b pcold-gst pcold I His GST pcold-gst b pcold-gst pcold I His Factor Xa human rhinovirus C protease HRV C GST HRV C GST PreScission protease pcold-gst pcold-gst kojima@bs.naist.jp NHE pcold-gst N C His N His a b NHE NMR c NMR NHE CHP α CHP NHE CHP NHE α
NHE MrkDaa GST-NHE SDS-PAGE S P W E GST-NHE b GST NHE SDS-PAGE NHE c MHz NMR NHE H- NHSQC NHE CHP PIP ERM CHP
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