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PAH? ZORBAX Eclipse Plus Eclipse Plus LC (PAH) C18 ZORBAX Eclipse Plus ZORBAX 2

ZORBAX Eclipse Plus LC Eclipse Plus Eclipse Plus LC ph ECLIPSE PAH C18 ZORBAX Eclipse Plus ( ) C18 Eclipse Plus HPLC HPLC Eclipse Plus 1. () 2. ( ) : 60/ 40 Eclipse Plus C18 Ace C18 SunFire C18 XBridge C18 Discovery HS C18 Luna C18 (2) Gei C18 XTerra MS C18 3

ZORBAX Eclipse Plus Eclipse Plus ZORBAX Eclipse C18 C8 Eclipse PAH µm HT (RRHT) Eclipse Plus Eclipse Eclipse Plus ZORBAX ZORBAX Eclipse Plus TEA : : :20% MeOH 80% 20 mm ph 7.0 = 1 ml/ :UV 24 ph (ph 2 9) ZORBAX Eclipse Plus ph 2.7 0.1% /ACN 1 Eclipse Plus C18 4.6 x 7 mm 3. µm 1. 2. 3. 2 3 4 6 N=7800 TF: 1.00 0 2 4 6 8 10 12 14 16 18 C18 4.6 x 7 mm 3. µm N=700 TF: 1.12 7 N=7800 TF: 1.0 N=4300 TF: 1.74 0 2 4 6 8 10 12 14 16 18 Eclipse Plus 3. µm 1. 2. 3. 4.. 6. 7. 0 2 4 6 8 ph 4.8 NH 4 AC/ACN 1 6 2 7 4 3 6 0 2 4 8 ph 7 NaPO 4 /ACN 7 1 3 2 6 ph ph Eclipse Plus 4 0 2 4 6 8 : : Eclipse Plus C18 4.6 x 100 mm µm : 10 10 90% : UV 24 nm 4

ZORBAX Eclipse ECLIPSE ZORBAX Eclipse Plus ZORBAX Eclipse Plus µm~7 µm ZORBAX RRHT Eclipse Plus µm 4.6 X 0 mm µ 1 Tf= 1.00 O O N + Cl OCH 3 OCH 3 0 0. 1 1. 2 2. 4.6 X 10 mm µ Tf= 1.06 : : 0%ACN: 0% 8mM K2HPO4 (ph7) : 1.0mL/ : 0.4mg/mL 2.0 µl: UV 24 nm 2? µm ZORBAX Eclipse Plus RRHT RRHT mau 80 60 40 20 0 231 bar 2. µm ZORBAX RRHT ZORBAX RRHT Eclipse Plus C18 2.1 x 30 mm µm N: 400 TF: 1.04 Rs 4,3 : 1.62 36% MeCN 64% 0.7 ml/l 2 µl 220 nm : 0. ml/ RRHT 0.4 ml/ (Luna) 1. 2 2(4 ) 2. 2(3) 3. 4. 2(4n ). 0.12 mg/ml mau 80 1 2 3 4 6 7 8 Phenomenex Luna HST C18 (2) 2.0 x 30 mm 2. µm 60 40 20 0 160 bar N: 3800 TF: 1.12 Rs 4,3 : 1.47 1 2 3 4 6 7 8 ZORBAX RRHT Luna HST C18 (2) 18% : Eclipse Plus C18 Luna HST C18 (2)

ZORBAX (RRHT) : 120 (Ultrafast) RRHT LC ZORBAX RRHT LC HPLC 20 9% LC LC/MS 120 1 mm 10 mm 2.1 mm 4.6 mm 12 ZORBAX ZORBAX Eclipse Plus C18 C8 PAH Eclipse XDBC18 C8 StableBond C18 C8 CN Phenyl AQ SBPhenyl SBAQ ExtendC18 ZORBAX Eclipse Plus RRHT µm Agilent 1200 RRLC LC20 LC 60% 600 30 60,000 Web Agilent 1200 Rapid Resolution LC (RRLC) ZORBAX RRHT µm LC All the performance. All the time. 6

ECLIPSE : ZORBAX Eclipse PAH 16 PAH (PAH) µm ( ) mau A 1 600 400 PAH 4.6 x 100 mm µm A 200 16 0 0 2. 7. 10 12. 1 17. 20 mau A 00 1 300 100 PAH 4.6 x 100 mm µm B 0 0 2. 7. 10 12. 1 17. 20 16 : Eclipse PAH 4.6 x 100 mm µm : A. 1. 2. 12. (k) 3. 4. 13. (a). 14. (a,h) 6. 7. 1. (ghi) 8. 16. (1,2,3cd) 9. (a) 10. 11. (b) : : 2 ml/ : %B 0.00 40% 0.66 40% 20 100% : A: B: ACN : UV 220 nm ZORBAX Eclipse Plus C18 4.6 x 0 mm µm 1. 2. 3. N: 8800 Pw: 0.014 TF: 1.20 N: 11600 Pw: 0.047 TF: 1.06 N: 11800 Pw: 0.093 TF: 1.02 0 1 2 3 4 Rapid Resolution Eclipse Plus C8 4.6 x 0 mm 3. µm N: 000 Pw: 0.019 TF: 1.09 N: 6600 Pw: 0.037 TF: 1.99 2.2 N: 6300 Pw: 0.061 TF: 0.98 : : : (30:70) : 2.0 ml/ : UV 230 nm : 30 : ACN (100 ACN 0.4 µm ) 0 1 2 3 4 RRHT C8 7

Eclipse Plus ZORBAX Eclipse Plus (mm) (µm) Eclipse Plus C18 USP L1 Eclipse Plus C8 USP L7 Eclipse Plus PAH ZORBAX Eclipse Plus Eclipse Plus ZORBAX Eclipse Plus LC 3. µm 9.4 x 20 4.6 x 20 4.6 x 10 4.6 x 100 4.6 x 0 4.6 x 10 4.6 x 10 4.6 x 100 4.6 x 7 4.6 x 0 4.6 x 30 4.6 x 100 4.6 x 0 4.6 x 30 3.0 x 20 3.0 x 10 3.0 x 10 3.0 x 10 3.0 x 100 3.0 x 100 3.0 x 0 2.1 x 20 2.1 x 10 2.1 x 10 2.1 x 10 2.1 x 100 2.1 x 0 2.1 x 0 2.1 x 30 2.1 x 100 2.1 x 0 2.1 x 30 4.6 x 12. 2.1 x 12. 3. 3. 3. 3. 3. 3. 3. 3. 3. 3. 3. 99990902 99993902 99996902 99946902 99963902 99994902 99961902 99933902 99943902 99936902 99964902 99941902 99931902 99993302 99963302 99994302 99961302 99964302 99941302 99701902 99763902 99794902 99793902 99746902 99743902 99733902 99764902 99741902 99731902 82090936 82112936 820888901 99990906 99993906 99996906 99946906 99963906 99961906 99933906 99943906 99936906 99964906 99941906 99931906 99993306 99963306 99961306 99964306 99941306 99701906 99763906 99793906 99746906 99743906 99733906 99764906 99741906 99731906 82090937 82112937 820888901 99990918 99993918 99996918 99963918 99961918 99943918 99964918 99941918 99931918 99990318 99790918 99701918 99793918 99764918 99741918 82090939 82112939 820888901 (0120477111 Eclipse Plus www.agilent.com/chem/jp ZORBAX 8

ZORBAX Eclipse LC ECLIPSE Eclipse LC Eclipse LC ph µm 7 µm 20 60% ZORBAX Rapid Resolution (RRHT) (600 bar) Eclipse LC Eclipse XDB ph (ph 2 ph 9) ZORBAX 4 Eclipse XDB ( 9894086EN) Eclipse XDB ZORBAX ZORBAX Eclipse XDB LC HPLC Eclipse XDB 1 9

ZORBAX RRHT µm LC ( : 600 bar 9,000 psi) LC Eclipse XDBC18 Eclipse XDBC8 SBC18 SBC8 SBCN ExtendC18 4.6 x 10 mm 4.6 x 100 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 3.0 x 0 mm 3.0 x 30 mm 3.0 x 20 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm 1.0 x 100 mm 1.0 x 0 mm 92897902 92797902 92497902 92697902 92897302 92797302 92497302 92697302 928700902 927700902 924700902 926700902 928600902 922600902 92897906 92797906 92497906 92697906 92897306 92797306 92497306 92697306 928700906 927700906 924700906 926700906 928600906 922600906 82997902 82897902 82797902 82497902 82697902 82997302 82897302 82797302 82497302 82697302 820700902 828700902 827700902 824700902 826700902 828600902 822600902 82997906 82897906 82797906 82497906 82697906 82997306 82897306 82797306 82497306 82697306 820700906 828700906 827700906 824700906 826700906 828600906 822600906 8299790 8289790 8279790 8249790 8299730 8289730 8279730 8249730 82070090 82870090 82770090 82470090 82860090 82260090 72897902 72797902 72497902 72697902 72897302 72797302 72497302 72697302 728700902 727700902 724700902 726700902 728600902 722600902 2007 ZORBAX RRHT µm ( : 400 bar 6,000 psi) LC Eclipse XDBC18 Eclipse XDBC8 SBC18 SBC8 ExtendC18 4.6 x 0 mm 2.1 x 0 mm 92297902 922700902 92297906 82297902 822700902 82297906 72297902 820901 4.6 x 0 mm 4.6 x 30 mm 4.6 x 1 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 1 mm 9297902 92397902 92197902 92700902 923700902 921700902 8297902 82397902 82197902 82700902 823700902 821700902 3 ZORBAX Eclipse LC : www.agilent.com/chem/jp 10

ZORBAX Eclipse Rapid Resolution 3. µm HPLC Eclipse XDBC18 Eclipse XDBC8 Eclipse XDBCN Eclipse XDBPhenyl 4.6 x 10 mm 4.6 x 100 mm 4.6 x 7 mm 4.6 x 0 mm 4.6 x 30 mm 4.6 x 20 mm 3.0 x 10 mm 3.0 x 100 mm 2.1 x 10 mm 2.1 x 100 mm 2.1 x 0 mm 2.1 x 30 mm 2.1 x 20 mm 1.0 x 10 mm 1.0 x 0 mm 1.0 x 30 mm 0. x 10 mm 0. x 3 mm 0.3 x 10 mm 4.6 x 7 mm 3.0 x 7 mm 963967902 961967902 966967902 93967902 934967902 932967902 96394302 961967302 930990902 96173902 971700902 974700902 972700902 963600902 96600902 961600902 963967906 961967906 966967906 93967906 934967906 932967906 96394306 961967306 930990906 96173906 971700906 974700906 972700906 963600906 96600906 961600906 96396790 96196790 96696790 9639430 9617390 963967912 966967912 93967912 96394312 961967312 0648288 0648298 0648271 4.6 x 12. mm, 4pk 2.1 x 12. mm, 4pk 4.6 & 2.1 mm 1.0 x 17 mm, 3pk 799118344 799230344 021184 8209092 82112926 820888901 18921 799108344 021184 82090926 82112926 820888901 18921 8209093 8211293 820888901 82090927 82112926 820888901 (0120477111) ZORBAX Eclipse µm HPLC 2007 21.2 x 10 mm 21.2 x 100 mm 21.2 x 0 mm 9.4 x 20 mm 4.6 x 20 mm 4.6 x 10 mm 4.6 x 0 mm 3.0 x 20 mm 3.0 x 10 mm 2.1 x 10 mm 2.1 x 10 mm 0. x 20 mm 0. x 10 mm 0. x 3 mm 0.3 x 20 mm 0.3 x 10 mm 0.3 x 3 mm 4.6 x 20 mm 4.6 x 10 mm 17 x 7. mm 4.6 x 12. mm, 4pk 4.0 x 4 mm, 10pk 2.1 x 12. mm, 4pk Eclipse XDBC18 Eclipse XDBC8 Eclipse XDBCN Eclipse XDBPhenyl 97010902 970100902 97000902 990967202 990967902 993967902 94697902 990967302 993967302 993700902 960967902 0648288 0648287 0648296 0648269 0648291 0648297 7991188 7991189 021184 82021292 8209092 79911804 82112926 97010906 970100906 97000906 990967206 990967906 993967906 94697906 990967306 993967306 993700906 960967906 7991088 7991089 021184 820212926 82090926 79911804 82112926 99096790 99396790 99096730 99396730 99370090 96096790 8209093 8211293 990967912 993967912 990967312 993967312 993700912 960967912 82090927 82112926 ZORBAX Eclipse 7 µm HPLC 21.2 x 20 mm 21.2 x 10 mm Eclipse XDBC18 Eclipse XDBC8 Eclipse XDBCN Eclipse XDBPhenyl 97720102 97710102 97720106 97710106 11

ZORBAX Eclipse LC 40 ZORBAX Eclipse Plus Eclipse LC : 0120477111 www.agilent.com/chem/jp 0120477111 Agilent 1200 Rapid Resolution LC (RRLC) URL : www.agilent.com/chem/1200hplc:jp Web Agilent Technologies, Inc. 2007 Printed in Japan October 31, 2007 9896436JAJP