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1 Jpn. J. Oral Biol., 39: , Effect of reserpine on pilocarpine-induced salivary secretion in mice Hiroshi Saito Department of Pharmacology, School of Dentistry, Iwate Medical University (Chief: Prof. Tadanobu Itoh) Chuodori, Morioka 020, Japan Key words: reserpine/pilocarpine-induced salivation/mouse Abstract: Effects of reserpine on the response to salivation induced by a parasympathomimetic, pilocarpine, in mice were examined. 1. The amount of saliva and the duration of salivation were significantly increased in a dose-dependent the response to pilocarpine-induced salivation 12 hours after the administration of reserpine (1mg/kg) showed a significant decrease compared to the control, whereas the salivary response 7 days after the administration of reserpine recovered to the same level as the control. 2. In response to pilocarpine-induced salivation, the increased salivary response 30 min after administration of reserpine (1mg/kg) was inhibited with a parasympathomimetic blocking agent, atropine, but not 3. The acetylcholine levels in the submandibular and sublingual glands of mice 30 min or 12 hours after the administration of reserpine did not differ from the control levels of the mice 12 hours after reserpine administration showed a significant increase 4. In the reserpinized mice, food consumption, water intake, whereas the choline levels in the glands., and body weight gain showed a reduction in the 1st and 2nd days after reserpine administration, but were recovered 7 days after administration. These findings suggest that in mice receiving a single administration of reserpine, the pilocarpine-induced salivary response displays a time-dependent biphasic magnitude: a dose-dependent increase 30 min afte r the administration of reserpine, and a decrease 12 hours after the administration of reserpine. In addition, this increase in the pilocarpine-induced salivary response suggests that the reactivity of the parasym pathetic nervous system accelerated, because its inhibition was dependent on the dosage of parasym pathomimetic

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10 adrenergic sialogogue-induced salivation in mice ) Mural, S., Miyate, H., Saito. H., Nagahama, H., Masuda, Y. and Itoh, T.: Simple determination of acetylcholine and choline within 4 min by HPLC -ECD and immobilized enzyme column in mice Marier, S.H.: Is calcium the final mediator exocytosis in the rat parotid gland? J. Pharmacol. 11) Rand, M.J. and Jurevics, H.: The pharmacology 12) McMahon, F.G.: Rauwolfia alkaloids and deriv- atives. In: Managment of Essential Hyperten- Futura Publishing Co. Inc., Mount Kisco, New 2) Martinez, J.R., Quissell, D.O., Wood, D.L. and Giles, M.: Abnormal secretory response to parasympathomimetic and sympathomimetic stimulation from the submaxillary gland of rats treated with reserpine. J. Pharmac. Exp. Ther. 194: 384 3) Jirakulsomchok, K.D., Yu, J.H. and Schneyer, C. D.: Secretory responses to autonomic stimulation of rat salivary glands following reserpine treat- 4) Jha, R.J. and Kelkar, V.V.: Effect of drugs interacting with adrenergic mechanisms and of adrenalectomy on pilocarpine-induced salivation 5) Potter, P.E., Meek, J.L. and Neff, N.H.: Acetylcholine and choline in neuronal tissues measured by HPLC with electrochemical detection. J. Neu- 6) Richter, W.: Estimation of anticholinergic drug effect in mice by antagonism against pilocarpine -induced salivation. Acta. Pharmacol. Toxical. 7) Mural, S., Saito, H., Masuda, Y., Nakamura, K., Yoshida, H. and Itoh, T.: A modified method for quantitative measurements of cholinergic and 9) Putney, J.W., Jr. Weiss, S.J., Leslie, B.A. and of rauwolfia alkaloids. In: Handbook of Experimental Pharmacology. (edited by Gross, F.) 39: York, ) Anand, B.K., Dua, S. and Malhotra, C.L.: Effects of reserpine on blood-pressure responses evoked from the hypothalamus. Brit. J. phar- 14) Malhotra, C.L. and Pundlik, P.G.: The effect of reserpine on the acetylcholine content of different areas of the central nervous system of the dog. 15) Malhotra, C.L. and Das, P.K.: Effect of reserpine on the acetylcholine content of the heart, the ileum and the hypothalamus of the dog. Brit. J. 16) Malpica, J.F., Jurupe, H. and Campos, H.A.: Action of reserpine and tyramine on the acetylcholine content of brain stem, heart and blood of ) Fathi, M.M. and Asaad, A.M.: Effect of tranquilizers on the total acetylcholine content and acetylcholinesterase activity in the brain tissue of arvicanthis niloticus. Comp. Biochem. Physiol.

11 Watts, J.S.: Comparative action of reserpine, aminyl cholesterol on the pilocarpine-induced tetrabenazine and chlorpromazine on central salivary response and salivary gland acetylcholine and choline level in mice. Jpn. J. Oral. size and lacrimation in rabbit and on salivation parasympathetic activity: Effects on pupillary in 21) Bianchi, C. and Beani, L.: Reserpine and the neuromuscular junction. J. Pharm. Pharmac. 18: 22) Malpica, J.F., Jurupe, H. and Campos, H.A.: Actions of reserpine and tyramine on the acetylcholine content of brain stem, heart and blood of ) Fathi, M.M.: Effect of drugs on brain acetylcholine level in gerbillus pyramidum. Comp. Bio- 24) Bodanskie, D.F., Sulser, F., Brodie, B.B. and 25) Martinez, J.R., Adelstei, E. Quissell, D. and Barbero, G.J.: The chronically reserpinized rat as a possible model for cystic fibrosis. I. Submaxillary gland morphology and ultrastructure. Pediat. Res. 26) Leibowitz, S.R.: Brain catecholaminergic mechanisms for control of hunger. In "Hunger: Basic Mechanisms and Clinical Implication" (Novin, D.,

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