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3 ヒ ト胃 型 物 質 の ガ ス ク ロ マ ト (377)

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10 1) Kabat, E. A.: Blood Group Substances, Their Chemistry and Immunochemistry, Academic Press, New York, 1956 ( , ). 2) Watkins, W. M. : Blood-Group Specific Substance, in Glycoproteins, Their Composition, Structure and Function, edited by Gottschlk, A., Elsvier Publ. Co., Amsterdam, London, New York, 1966 ( ). 3) Iseki, S. : Glycosidases and Serological Changes in Blood Group Substances, in Blood and Tissue Antigens, edited by Aminoff, D., Academic Press, 1970 ( ). 4) Matsui, M., Okada, M., Imanari, T., and Tamura, Z. : Gas chromatography of trifluoroacetyl derivatives of alditols and trimethylsilyl derivatives of aldonolactones, Chem, Pharr. Bull., 16(7) : , 6) Tamura, Z., Imanari, T., and Arakawa, Y. : Gas chromatography of principal hexosamines, Chem. Pharm. Bull., 16(9) , ) Benclich, A., Kabat, E. A., and Bezer, A. E. : Immunochemical studies on blood groups. III. Properties of purified blood group A substances from individual hog stomach linings, J. Exp. Med., 83(6) , ) Morgan, W. T. J. and King, H. K. : Studies in immunochemistry. 7. The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A specificity, Biochem. J., 37(5) : , ) Svernnerholm, L. : Quantitative estimation of sialic acids. II A colorimetric resorcinolhydrochloric acid methods, Biochim. Biophys, Acta, 24(3) : , ) Spiro, R. G.: Analysis of sugars found in glycoproteins, in Methods in Enzymology, Vol. VIII, Complex Carbohydrates, edited by Neufeled, E. F. and Ginsburg, V. : Academic Press, New York and London, 1966 (15-16). 14) Masamune, H., Yoshizawa, Z., and Masukawa, A.: Comparison of the group carbohy

11 drate of gastric cancer with the corresponding carbohydrate of gastric mucosa, Tohoku J. Exp. Med., 58(3-4) : , ) Schrager, J. and Oates, M. D. G. : The carbohydrate components of hydrolysates of gastric secretion and extracts from mucus glands of the gastric body mucosa and antrum, Biochem. J., 106(2) : , ) Masamune, H. and Tsuiki, S. : Biochemical studies on carbohydrates. CCX. A systematic fractionation of pig gasric mucosa with respect to Molish-positive hexosaminecontaining components, Tohoku J. Exp. Med., 67(2-3) : , ) Hiyama, N. : Chemical and serological studies on blood group active mucopolysaccharides, in Biochemistry and Medicine of mucopolysaccharides, edited by Egami, F. and Oshima, Y., Maruzen Co. Ltd., Tokyo, 1962 ( ). 19) Nemoto, T. and Yosizawa, Z: A glycopeptide and glycosaminoglycan-peptides isolated from rabbit gastric mucosa, J. Biochem,, 66(5) : , ) Masamune, H., Yosizawa, Z., and Tokita. K.: Biochemical studies on carbohydrates. CXCVI. Comparison among blood group A, B and O mucopolysaccharides from human gastric juices, Tohoku J. Exp. Med. 65(2-3) : , ) Schrager, J: Chromatographic observations of human gastric mucopolysaccharides, Nature, 198(4883) : , ) Gheorghiu, Th. und Oette, K.: Die Gaschromatographische Analyse von Zuckern und Zuckerderi vaten aus dialysierbaren Substanzen biologischer Flussigkeiten, J. Chromatog., 48(3) : , 1970.

12 CONSTITUENT SUGAR ANALYSIS OF PHENOL-INSOLUBLE BLOOD GROUP SUBSTANCES FROM HUMAN GASTRIC MUCOSA BY AN IMPROVED GAS CHROMATOGRAPHY SHIGERU YAMAMOTO Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan (Director : Prof. Shoei Iseki) Gas chromatographic determination of neutral sugars reduced by NaBH4 as well as aminosugars N-acetylated with acetic anhydride and reduced by NaBH4 were improved in a system using trifluoroacetyl derivatives of their sugar alcohols in which a glass column was packed with 2 % Silicone XF-1105 on Gas Chrom P. Constituent sugars in blood group substances from human gastric mucosa were quantitatively determined by the improved method. In addition to fucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine as major component sugars, ribose, xylose, mannose, and glucose were confirmed to be minor component sugars in all samples with blood group activities and in sub-fractions separated from phenol-insoluble B-active glycoproteins, too. Sialic acid was colorimetrically found to be present in tiny amounts in all samples tested but not uronic acids.

Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of

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