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3 ヒ ト胃 型 物 質 の ガ ス ク ロ マ ト (377)
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10 1) Kabat, E. A.: Blood Group Substances, Their Chemistry and Immunochemistry, Academic Press, New York, 1956 ( , ). 2) Watkins, W. M. : Blood-Group Specific Substance, in Glycoproteins, Their Composition, Structure and Function, edited by Gottschlk, A., Elsvier Publ. Co., Amsterdam, London, New York, 1966 ( ). 3) Iseki, S. : Glycosidases and Serological Changes in Blood Group Substances, in Blood and Tissue Antigens, edited by Aminoff, D., Academic Press, 1970 ( ). 4) Matsui, M., Okada, M., Imanari, T., and Tamura, Z. : Gas chromatography of trifluoroacetyl derivatives of alditols and trimethylsilyl derivatives of aldonolactones, Chem, Pharr. Bull., 16(7) : , 6) Tamura, Z., Imanari, T., and Arakawa, Y. : Gas chromatography of principal hexosamines, Chem. Pharm. Bull., 16(9) , ) Benclich, A., Kabat, E. A., and Bezer, A. E. : Immunochemical studies on blood groups. III. Properties of purified blood group A substances from individual hog stomach linings, J. Exp. Med., 83(6) , ) Morgan, W. T. J. and King, H. K. : Studies in immunochemistry. 7. The isolation from hog gastric mucin of the polysaccharide-amino acid complex possessing blood group A specificity, Biochem. J., 37(5) : , ) Svernnerholm, L. : Quantitative estimation of sialic acids. II A colorimetric resorcinolhydrochloric acid methods, Biochim. Biophys, Acta, 24(3) : , ) Spiro, R. G.: Analysis of sugars found in glycoproteins, in Methods in Enzymology, Vol. VIII, Complex Carbohydrates, edited by Neufeled, E. F. and Ginsburg, V. : Academic Press, New York and London, 1966 (15-16). 14) Masamune, H., Yoshizawa, Z., and Masukawa, A.: Comparison of the group carbohy
11 drate of gastric cancer with the corresponding carbohydrate of gastric mucosa, Tohoku J. Exp. Med., 58(3-4) : , ) Schrager, J. and Oates, M. D. G. : The carbohydrate components of hydrolysates of gastric secretion and extracts from mucus glands of the gastric body mucosa and antrum, Biochem. J., 106(2) : , ) Masamune, H. and Tsuiki, S. : Biochemical studies on carbohydrates. CCX. A systematic fractionation of pig gasric mucosa with respect to Molish-positive hexosaminecontaining components, Tohoku J. Exp. Med., 67(2-3) : , ) Hiyama, N. : Chemical and serological studies on blood group active mucopolysaccharides, in Biochemistry and Medicine of mucopolysaccharides, edited by Egami, F. and Oshima, Y., Maruzen Co. Ltd., Tokyo, 1962 ( ). 19) Nemoto, T. and Yosizawa, Z: A glycopeptide and glycosaminoglycan-peptides isolated from rabbit gastric mucosa, J. Biochem,, 66(5) : , ) Masamune, H., Yosizawa, Z., and Tokita. K.: Biochemical studies on carbohydrates. CXCVI. Comparison among blood group A, B and O mucopolysaccharides from human gastric juices, Tohoku J. Exp. Med. 65(2-3) : , ) Schrager, J: Chromatographic observations of human gastric mucopolysaccharides, Nature, 198(4883) : , ) Gheorghiu, Th. und Oette, K.: Die Gaschromatographische Analyse von Zuckern und Zuckerderi vaten aus dialysierbaren Substanzen biologischer Flussigkeiten, J. Chromatog., 48(3) : , 1970.
12 CONSTITUENT SUGAR ANALYSIS OF PHENOL-INSOLUBLE BLOOD GROUP SUBSTANCES FROM HUMAN GASTRIC MUCOSA BY AN IMPROVED GAS CHROMATOGRAPHY SHIGERU YAMAMOTO Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan (Director : Prof. Shoei Iseki) Gas chromatographic determination of neutral sugars reduced by NaBH4 as well as aminosugars N-acetylated with acetic anhydride and reduced by NaBH4 were improved in a system using trifluoroacetyl derivatives of their sugar alcohols in which a glass column was packed with 2 % Silicone XF-1105 on Gas Chrom P. Constituent sugars in blood group substances from human gastric mucosa were quantitatively determined by the improved method. In addition to fucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine as major component sugars, ribose, xylose, mannose, and glucose were confirmed to be minor component sugars in all samples with blood group activities and in sub-fractions separated from phenol-insoluble B-active glycoproteins, too. Sialic acid was colorimetrically found to be present in tiny amounts in all samples tested but not uronic acids.
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