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1 N AMY BD BBLCRYSTAL N/H 1960 BD BBLCRYSTAL N/H NeisseriaHaemophilus BD BBLCRYSTAL N/H 4-A 2-A 1-A 4-B 2-B 1-B 4-C 2-C 1-C 4-D 2-D 1-D 4-E 2-E 1-E 4-F 2-F 1-F 4-G 2-G 1-G 4-H LYSALA N--ALA-PRO-ALA ALA-ALA-PHE -AMC GLY-PRO -D- g/ L L L L N L L L L D D D
2 2-H 1-H 4-I 2-I 1-I 4-J 2-J 1-J p-n-p- -p- p-n-p- o-n-p--d- g/ D p L---p p o--d L BD BBLCRYSTAL N/H Aggregatibacter actinomycetemcomitans Aggregatibacter segnis 1 Cardiobacterium hominis 1 Eikenella corrodens Gardnerella vaginalis Aggregatibacter aphrophilus Haemophilus ducreyi Haemophilus haemoglobinophilus 1 Haemophilus haemolyticus Heamophilus influenzae Haemophilus parahaemolyticus 1 Haemophilus parainfluenzae Kingella denitrificans Kingella kingae Moraxella atlantae Moraxella catarrhalis Moraxella lacunata 1 Moraxella nonliquefaciens Moraxella osloensis Psychrobacter phenylpyruvica 1 Neisseria cinerea 1 Neisseria elongata Neisseria flavescens 1 Neisseria gonorrhoeae Neisseria lactamica Neisseria meningitidis Neisseria mucosa Neisseria sicca Neisseria subflava Neisseria weaveri 1 Oligella ureolytica 1 Oligella urethralis Pasteurella multocida Suttonella indologenes 1=BBLCRYSTAL10 2
3 2BD BBLCRYSTAL N/H 4A FCT 2A FHO 1A FPR 4B FSE 2B LYS-ALA FLA 1B FTR 4C 2C 1C 4D N--ALA-PRO-ALA ALA-ALA-PHE FPH FNS FAA FTA D FAR 1D -AMC FOR 4E FGL 2E GLY-PRO FGP 1E -D- FBG 4F SAC 2F MTT 1F CAR 4G 2G PYO MTB ph G DIS 4H RBL 2H LEV 1H p-n-p- PHC p I -p- GGL p I 1I p-n-p- o-n-p--d- PHO OPG p J URE ph J REZ 6 1J ORN ph 2 3
4 3BD BBLCRYSTAL N/H 4A FCT 2A FHO FCT FCT 1A FPR FCT FCT 4B FSE FCT FCT 2B LYS-ALA FLA FCT FCT 1B FTR FCT FCT 4C FPH FCT FCT 2C N--ALA-PRO-ALA FNS FCT FCT 1C ALA-ALA-PHE FAA FCT FCT 4D FTA FCT FCT 2D FAR FCT FCT 1D -AMC FOR FCT FCT 4E FGL FCT FCT 2E GLY-PRO FGP FCT FCT 1E -D- FBG FCT FCT 4F SAC / / 2F MTT / / 1F CAR / / 4G PYO / / 2G MTB / / 1G DIS / / 4H RBL / / 2H LEV / / 1H p-n-p- PHC 4I -p- GGL 2I p-n-p- PHO 1I o-n-p--d- OPG 4J URE / / 2J REZ / 1J ORN / 2 8 BBLCRYSTAL BBLCRYSTAL ANR, GP, RGP, N/H BD BBLCRYSTAL N/H
5 BBLCRYSTAL ANR, GP, RGP, N/H % 1.0mL # mL BBLCRYSTAL N/H 3 3 5
6 CO % 2 N/H BBLCRYSTAL 3 BBL- CRYSTAL N/H F J 2 UV A E 4A BD BBLCRYSTAL 4A A B C D E F G H I J *4A 6
7 BBLCRYSTAL BBLCRYSTAL ANR, GP, RGP, N/H mL 20 1 KCl 7.50g CaCl N BBLCRYSTAL N/H 1 CO % #3.0BBLCRYSTAL BBLCRYSTAL 5TSA 5% MTM NYCV G. vaginalis GC %BD BBLCRYSTAL Manual of Clinical Microbiology 117 1BBLCRYSTAL N/H Moraxella (Branhamella) catarrhalis ATCC BBLCRYSTAL N/H 4 BBLCRYSTAL N/H 1 2 1J BBLCRYSTAL N/H
8 4BD BBLCRYSTAL N/H 4 8 4A 2A 1A 4B 2B 1B 4C 2C 1C 4D 2D 1D 4E 2E 1E 4F 2F 1F 4G 2G 1G 4H 2H 1H 4I 2I 1I 4J 2J 1J LYS-ALA N--ALA-PRO-ALA ALA-ALA-PHE -AMC GLY-PRO -D- p-n-p- -p- p-n-p- o-n-p--d- FCT FHO FPR FSE FLA FTR FPH FNS FAA FTA FAR FOR FGL FGP FBG SAC MTT CAR PYO MTB DIS RBL LEV PHC GGL PHO OPG URE REZ ORN + v v v v + + v Moraxella (Branhamella) catarrhalis ATCC V
9 5BD BBLCRYSTAL N/H 4 9 LYS-ALA N--ALA-PRO-ALA ALA-ALA-PHE -AMC GLY-PRO -D- p-n-p- -p- p-n-p- o-n-p--d- FCT FHO FPR FSE FLA FTR FPH FNS FAA FTA FAR FOR FGL FGP FBG SAC MTT CAR PYO MTB DIS RBL LEV PHC GGL PHO OPG URE REZ ORN Haemophilus aphrophilus ATCC Neisseria lactamica ATCC Kingella denitrificans ATCC Haemophilus influenzae ATCC V
10 3N/H %BD BBLCRYSTAL N/H 95.9% BD BBLCRYSTAL N/H 1 Neisseria gonorrhoeae BBL BD BBLCRYSTAL N/H TSA, MTM, NYC, V, GC BD BBLCRYSTAL N/H BD BBLCRYSTAL N/H 1 BBLCRYSTALi ii iii 3 10
11 Balows, A., W.J. Hausler, Jr., K.L. Herrmann, H.D. Isenberg, and H.J. Shadomy (ed.) Manual of clinical microbiology, 5 th ed. American Society for Microbiology, Washington, D.C. 2Baron, E.J., L.R. Peterson, and S.M. Finegold Bailey and Scott s diagnostic microbiology, 9 th ed. Mosby- Year Book, Inc., St. Louis. 3Bronfenbrenner, J., and M.J. Schlesinger A rapid method for the identification of bacteria fermenting carbohydrates. Am. J. Public Health. 8: Cowan, S.T., and K.J. Steel Manual for the identification of medical bacteria. 2 nd ed. Cambridge University Press, Cambridge. 5Edberg, S.C., and C.M. Kontnick Comparison of -glucuronidase-based substrate systems for identification of Escherichia coli. J. Clin. Microbiol. 24: Enriquez, L.A., and N.E. Hodinka The development of a test system for the rapid differentiation of Neisseria and Haemophilus. J. Clin. Microbiol. 18: Ferguson, W.W.,and A.E. Hook, Urease activity of Proteus and Salmonella organisms. J. Lab. Clin. Med. 28: Hartman, P.A Miniaturized microbiological methods. Academic Press, New York. 9Kämpfer, P., O. Rauhoff, and W. Dott Glycosidase profiles of members of the family Enterobacteriaceae. J. Clin. Microbiol. 29: Killian, M., and P. Bulow Rapid diagnosis of Enterobacteriaceae 1: detection of bacterial glycosidases. Acta Pathol. Microbiol. Scand. Sect. B. 84: Knapp, J.S., and R.J. Rice Neisseria and Branhamella, p In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (ed.), Manual of clinical microbiology, 6 th ed. American Society for Microbiology, Washington, D.C. 12MacFaddin, J.F Biochemical tests for identification of medical bacteria, 2 nd ed. Williams & Wilkins, Baltimore. 13Maddocks, J.L., and M. Greenan Rapid method for identifying bacterial enzymes. J. Clin. Pathol. 28: Manafi, M., W. Kneifel, and S. Bascomb Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol. Rev. 55: Mandell, G.L., R.G. Douglas, Jr. and J.E. Bennett Principles and practice of infectious diseases, 3 rd ed. Churchill Livingstone Inc., New York. 16Mangels, J.,I. Edvalson, and M. Cox Rapid identification of Bacteroides fragilis group organisms with the use of 4-methylumbelliferone derivative substrates. Clin. Infect. Dis. 16(54): Moncla, B.J., P. Braham, L.K. Rabe, and S.L. Hiller Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol. 29: Murray, P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (ed.) Manual of clinical microbiology, 6 th ed. American Society for Microbiology, Washington, D.C. 11
12 BD BD TEL FAX
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111-245130-N-03 21400AMY00158000 20099 1 BD BBLCRYSTAL N/H BD BBLCRYSTAL N/H 4-A g/ 7--4-0.175 2-A 4-- 1.501 1-A L--7--4-1.766 4-B L--7--4-1.4935 2-B LYSALA --7--4-2.237 1-B L--7--4-2.295 4-C L--7--4-2.182
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