N BD BBL GP 1960 BD BBL GP BBL GP BD BBL GP 4-A 2-A -D- 1-A 4-B 2-B -D- 1-B 4-C 2-C -AMC 1-C N- - -D- 4-D 2-D -D- 1-D 4-E 2-E 1-

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1 N BD BBLGP 1960 BD BBL GPBBL GP BD BBL GP 4-A 2-A -D- 1-A 4-B 2-B -D- 1-B 4-C 2-C -AMC 1-C N---D- 4-D 2-D -D- 1-D 4-E 2-E 1-E 4-F 2-F 1-F 4-G 2-G 1-G 4-H p-n-p--d- 2-H p-n-p--d- 1-H -p- 4-I p-n-p- 2-I p-n-p--d- g/ D L L D L L L N---D D L D D D L * 4.5 D p--d p--d L-p L--p p p--d

2 1-I 4-J 2-J 1-J o-n-p--d- p-n-p--d- g/ o--d p--d L *L/ 1BD BBL GP Aerococcus urinae Aerococcus viridans Alloiococcus otitis Arcanobacterium pyogenes Arcanobacterium haemolyticum a Bacillus aneurinolyticus Bacillus badius Bacillus cereus Bacillus circulans Bacillus coagulans Bacillus firmus Bacillus freundenreichii Bacillus lentus Bacillus licheniformis Bacillus megaterium Bacillus pumilus Bacillus sphaericus Bacillus subtilis Brevibacillus brevis Brevibacillus laterosporus Corynebacterium bovis Corynebacterium diphtheriae Corynebacterium genitalium Corynebacterium jeikeium Corynebacterium kutscheri Corynebacterium propinquum Corynebacterium pseudodiphtheriticum Corynebacterium pseudogenitalium Corynebacterium pseudotuberculosis Corynebacterium renale group Corynebacterium striatum Corynebacterium ulcerans Enterococcus avium Enterococcus casseliflavus/gallinarum Enterococcus durans Enterococcus faecalis Enterococcus faecium Enterococcus hirae Enterococcus raffinosus Erysipelothrix rhusiopathiae Gardnerella vaginalis Gemella haemolysans Gemella morbillorum Globicatella sanguis Helcococcus kunzii Kocuria kristinae Kocuria roseus Kytococcus sedentarius Lactococcus gravieae Lactococcus lactis ssp. cremoris Lactococcus lactis ssp. hordniae Lactococcus lactis ssp. lactis Lactococcus raffinolactis Leifsonia aquatica Leuconostoc citreum Leuconostoc lactis Leuconostoc mesenteroides spp. mesenteroides Leuconostoc pseudomesenteroides Listeria grayi a Listeria ivanovii ssp. ivanovii Listeria monocytogenes Listeria murrayi Micrococcus luteus Micrococcus lylae Oerskovia species Paenibacillus alvei Paenibacillus macerans Paenibacillus polymyxa Paenibacillus thiaminolyticus Pediococcus damnosus Pediococcus parvulus Pediococcus pentosaceus Rhodococcus equi Rothia dentocariosa a Rothia mucilaginosa Staphylococcus aureus Staphylococcus auricularis Staphylococcus capitis Staphylococcus caprae Staphylococcus carnosus Staphylococcus cohnii ssp. cohnii Staphylococcus cohnii ssp. urealyticum Staphylococcus epidermidis Staphylococcus equorum Staphylococcus felis Staphylococcus gallinarum Staphylococcus haemolyticus Staphylococcus hominis Staphylococcus intermedius Staphylococcus kloosii Staphylococcus lentus Staphylococcus lugdunensis Staphylococcus pasteuri a Staphylococcus saccharolyticus Staphylococcus saprophyticus Staphylococcus schleiferi Staphylococcus sciuri Staphylococcus simulans Staphylococcus vitulinus Staphylococcus warneri Staphylococcus xylosus Streptococcus acidominimus Streptococcus agalactiae Streptococcus anginosus Streptococcus bovis Streptococcus constellatus Streptococcus criceti a Streptococcus cristatus Streptococcus dysgalactiae Streptococcus equi ssp. equi Streptococcus equi ssp. zooepidemicus Streptococcus equinus Streptococcus gordonii Streptococcus intermedius Streptococcus mitis Streptococcus mutans Streptococcus oralis Streptococcus parasanguis Streptococcus pneumoniae Streptococcus porcinus Streptococcus pyogenes Streptococcus salivarius Streptococcus sanguinis Streptococcus sobrinus Streptococcus uberis Streptococcus vestibularis Tetragenococcus solitarius Turicella otitidis a irgibacillus pantothenticus a 2

3 2BD BBL GP 4-A FCT 2-A -D- FGC 1-A FA 4-B FPH 2-B -D- FGS 1-B 4-C 2-C -AMC FPY FTR FAR C N---D- FGA 4-D FHO 2-D -D- FGN 1-D FIS 4-E TRE 2-E LAC 1-E MAB 4-F 2-F 1-F SUC MNT MTT ph G ARA 2-G GLR 1-G FRU 4-H 2-H p-n-p--d- p-n-p--d- BGL PCE p H -p- PLN p I 2-I 1-I p-n-p- p-n-p--d- o-n-p--d-p-n-p--d- PHO PAM PGO p J URE ph J ESC 10 1-J ARG ph 2 3

4 4 4-A 2-A 1-A 4-B 2-B 1-B 4-C 2-C 1-C 4-D 2-D 1-D 4-E 2-E 1-E 4-F 2-F 1-F 4-G 2-G 1-G 4-H 2-H 1-H 4-I 2-I 1-I 4-J 2-J 1-J FCT FGC FA FPH FGS FPY FTR FAR FGA FHO FGN FIS TRE LAC MAB SUC MNT MTT ARA GLR FRU BGL PCE PLN PHO PAM PGO URE ESC ARG -D- -D- -AMC N---D- -D- p-n-p--d- p-n-p--d- -p- p-n-p- p-n-p--d- o-n-p--d- p-n-p--d- FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT / FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT FCT / / / / / / / / / / / 3BD BBL GP 28 BBL BBL ANR, GP, RGP, N/H

BBL GP 2 8 2 8 225 1525 1 1 2 1 3BBL ANR, GP, RGP, N/H

5 BBL GP BBL ANR, GP, RGP, N/H #0.5#0.5 a #0.5 b 0.85% 1.0mL# mL

6 CO % 2 BBL GP BBL 3BBL GP a EJ b UAD 4A BD BBL 4A A B C D E F G H I J *4A 6

7 BBL BBLANR, GP, RGP, N/H mL 20 1 KCl 7.50g CaCl 2 0.5g N g 2 1 CO % #0.5BBLBBL 1BBL GPStreptococcus pyogenes ATCC BBL GP J BBLGP

8 8 4-A 2-A 1-A 4-B 2-B 1-B 4-C 2-C 1-C 4-D 2-D 1-D 4-E 2-E 1-E 4-F 2-F 1-F 4-G 2-G 1-G 4-H 2-H 1-H 4-I 2-I 1-I 4-J 2-J 1-J FCT FGC FA FPH FGS FPY FTR FAR FGA FHO FGN FIS TRE LAC MAB SUC MNT MTT ARA GLR FRU BGL PCE PLN PHO PAM PGO URE ESC ARG * Streptococcus pyogenes ATCC * BD BBL GP TSA 18 20

9 9 4-A 2-A 1-A 4-B 2-B 1-B 4-C 2-C 1-C 4-D 2-D 1-D 4-E 2-E 1-E 4-F 2-F 1-F 4-G 2-G 1-G 4-H 2-H 1-H 4-I 2-I 1-I 4-J 2-J 1-J FCT FGC FA FPH FGS FPY FTR FAR FGA FHO FGN FIS TRE LAC MAB SUC MNT MTT ARA GLR FRU BGL PCE PLN PHO PAM PGO URE ESC ARG * * * Staphylococcus epidermidis ATCC Bacillus brevis ATCC 8246 Enterococcus faecalis ATCC Staphylococcus xylosus ATCC * BD BBL GP TSA 18 20

10 BBL GP1 BBL BBL GPTSA, PEACNA BBL GP BBL GP 1 BBLi ii iii Balows, A., W.J. Hausler, Jr., K.L. Herrmann, H.D. Isenberg, and H.J. Shadomy (ed.) Manual of clinical microbiology, 5 th ed. American Society for Microbiology, Washington, D.C. 2Baron, E.J., L.R. Peterson, and S.M. Finegold Bailey and Scott s diagnostic microbiology, 9 th ed. Mosby- Year Book, Inc., St. Louis. 3Bronfenbrenner, J., and M.J. Schlesinger A rapid method for the identification of bacteria fermenting carbohydrates. Am. J. Public Health. 8: Cowan, S.T., and K.J. Steel Manual for the identification of medical bacteria. 2 nd ed. Cambridge University Press, Cambridge. 10

11 5Edberg, S.C., and C.M. Kontnick Comparison of -glucuronidase-based substrate systems for identification of Escherichia coli. J. Clin. Microbiol. 24: Ferguson, W.W.,and A.E. Hook, Urease activity of Proteus and Salmonella organisms. J. Lab. Clin. Med. 28: Hartman, P.A Miniaturized microbiological methods. Academic Press, New York. 8Käpfer, P., O. Rauhoff, and W. Dott Glycosidase profiles of members of the family Enterobacteriaceae. J. Clin. Microbiol. 29: Killian, M., and P. Bulow Rapid diagnosis of Enterobacteriaceae 1: detection of bacterial glycosidases. Acta Pathol. Microbiol. Scand. Sect. B. 84: MacFaddin, J.F Biochemical tests for identification of medical bacteria, 2 nd ed. Williams & Wilkins, Baltimore. 11Maddocks, J.L., and M. Greenan Rapid method for identifying bacterial enzymes. J. Clin. Pathol. 28: Manafi, M., W. Kneifel, and S. Bascomb Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiol. Rev. 55: Mangels, J.,I. Edvaslon, and M. Cox Rapid identification of Bacteroides fragilis group organisms with the use of 4-methylumbelliferone derivative substrates. Clin. Infect. Dis. 16(54): Moncla, B.J., P. Braham, L.K. Rabe, and S.L. Hiller Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol. 29: Murray, P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (ed.) Manual of clinical microbiology, 6 th ed. American Society for Microbiology, Washington, D.C. 11

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N-03.indd 111-24514035-N-03 21300AMY00325000 20099 1 BD BBLCRYSTAL GP BD BBLCRYSTAL GP g/ 4-A 7--4-0.175 2-A -D- 4---D- 1.692 1-A L--7--4-1.7775 4-B L--7--4-2.182 2-B -D- 4---D- 1.6915 1-B L--7--4-1.4315 4-C L--7--4-2.3815