本体/01‐近泉・中村・水谷
|
|
- あきみ にかどり
- 5 years ago
- Views:
Transcription
1 Mem. Fac. Agr., Ehime Univ. Saccharomyces cerevisiae Hong mei ZHAO, Yudai SHIRAISHI, Hiroki TANAKA and Yasuo WATANABE : Purification of Glycerol Dehydrogenases from Saccharomyces cerevisiae and the Substrate Specificity of Bacterial Enzymes Abstract We have obtained the dehydrogenases being specific for mannitol and arabitol with the gene cloning and over-expression in yeast cells, in order to measure the content of various sugar alcohols by using the dehydrogenases. In this study, we carried out the isolations of glycerol dehydrogenases from Saccharomyces cerevisiae and determined its substrate specificities. Target proteins were purified by affinity chromatography with the addition of tag at N-terminus or C-terminus by gene recombination. The obtained enzymes showed the low specificity for sugar alcohols. However, we found the bacterial enzymes that showed the high specificity for glycerol in commercial products. These glycerol dehydrogenases could be available in the enzymatic analysis of glycerol content. polyhydroxyalkane D D DD β Onishi van Zyl and Prior D D D foods/index.html Onishi van Zyl and Prior Onishi Iwaki et al.watanabe et al. Watanabe et al.zygosaccharomyces rouxii Saccharomyces cerevisiae Iwaki et al.z. rouxii van Zyl et
2 al. Tang et al. de Figueroa and Lucca S. cerevisiae ORF YEL070w Watanabe et al. Minamihara and Suzuki Kluyveromyces lactis D S. cerevisiae Dujon et al. Gelperin et al. S. cerevisiae GCY1 YPR1 N C S. cerevisiae GCY1 YPR1 ORF BG His peghs ORF S S-Powder - ura Difco g g Ile g Val g Ade g Arg g His g Leu g Lysg Met g Phe g Trp g Tyr g SC ura SC - ura ml S-Powder - ura g g SC - ura ml S-Powder - ura g gg YP ml Difco gbecton Dickinson g g SC - ura GCY1 YPR1 SC - ura ml ml SC - ura ml OD nm YP ml SC - ura ml ml ml OD nm g g mlmm KCl mm phml
3 Saccharomyces cerevisiae g Vibrogen- Zellumuhle g g μm Bio-Rad Profinia ml IMAC Bio-Rad ml GST Bio- Rad Profinia Profinia μlμl μl Bio-Rad TGX V ORIOLE Bio-Rad SDS-PAGE ma PVDF HA Open Biosystems Invitrogen Western Breeze ml ml mm phml M ml M ml mm NAD ml ml U nm S. cerevisiae GCY1 YPR1 S. cerevisiae S. cerevisiae GCY1 YPR1 Costenoble et al. S. cerevisiae ORF BG Gelperin et al. BG GAL1 Gelperin et al. GCY1 YPR1 GCY1 GCYYPR1 YPR ph ph Cheng et al.hallborn et al.linlink et al.stein et al. Wong et al. GCY YPR GCY YPR His GCY1 YPR1 BG ORF His HA Protein A ZZ C GCY YPRBio-Rad
4 His Ni Profinia A B GCY1 C YPR1 A BC D nm Ni mlnm Profinia IMAC nm AB C D A D B GCY1 GCY D C YPR1 GCY1 GCY SDS-PAGE GCY1 KDa His KDa KDa D HA GCY YPR D GCY1 Profinia SDS MA B C D Profinia
5 Saccharomyces cerevisiae GCY1 YPR1 Profinia A GCY1 B YPR1 MA B C D Profinia GCY1 YPR1 A GCY1 B YPR1 D His GCY YPR Ni His GCY YPR GCY
6 Cellulomonas sp. G Enterobacter aerogenes G A Cellulomonas sp. GB Enterbacter aerogenes G YPR GST YPR1 pegh ORF N S GSTHis YPR1 GST GST-YPR GST-YPR A Cellulomonas sp. G B Enterobactor aerogenes G Oliveira et al.dominguez et al. Saccharomyces cerevisiae Kluyvermyces lactis S. cerevisiae N C Cheng H, Jiang N, Shen A, and Feng YMolecular cloning and functional expression of D-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli. FEMS Microbiol. Lett. Costenoble R, Valadi H, Gustafsson L, Niklasson C, and Franzen CJ Microaerobic glycerol formation in Saccharomyces cerevisiae. Yeast
7 Saccharomyces cerevisiae de Figueroa LIC and Lucca MEProdction of polyols by omsmotolerant yeasts. Food Microbiol. Protocols Dominguez KBH, Toth IV, et al.sequential injection kinetic flow assay for monitoring glycerol in a sugar fermentation process by Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. Dujon B et al.genome evolution in yeasts. Nature Gelperin DM, White MA, et al. Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes Developm. Hallborn J, Walfridsson M, Penttila M, Keranen S, and Hahn- Hagerdal B A Short-chain dehydrogenase gene from Pichia stipites having D-arabitoldehydrogenase activity. Yeast Iwaki T, Kurono S, Yokose Y, Kubota K, Tamai Y, and Watanabe Y Cloning of glycerol- -phosphate dehydrogenase gene ZrGPD1 and ZrGPD2 and glycerol dehydrogenase genes ZrGCY1 and ZrGCY2 from the salt-tolerant yeast Zygosaccharomyces rouxii. Yeast Lin ECCAn inducible D-arabitol dehydrogenase from Aerobacter aerogenes. J.Biol.Chem. Link T, Lohaus G, Heiser I, Mendgen K, Hahn M, and Voegele RTCharacterization of a novel NADP + dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae. Biochem. J. Minamihara T and Suzuki MProperties of sorbitol dehydrogenase from Pseudomonas sp. KS-E and comparison with other sorbitiol dehydrogenases. J. Ferment. Bioeng. Oliveira HM, Segundo MA, et al.kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection. J. Agric. Food Chem. Onishi HOsmophillic yeast. Adv. Food Res. Stein R, Gross W, and Schnarrenberger CCharacterization of a xylitol dehydrogenase and a D-arabitol dehydrogenase from the thermo- and acidophilic red alga Galdieria sulphuraria. Planta Tang XM, Kayingo G, and Prior BA Functional analysis of the Zygosaccharomyces rouxii Fps1p homologue. Yeast van Zyl PJ and Prior BAWater relations of polyol accumulation by Zygosaccharomyces rouxii in continuous culture. Appl. Microbiol. Biotechnol. van Zyl PJ, Kilian SG, and Prior PATheroleofan active transport mechanism in glycerol accumulation during osmoregulation by Zygosaccharomyces rouxii. Appl. Microbiol. Biotechnol. Watanabe Y, Nagayama K, and Tamai YExpession of glycerol -phosphate dehydrogenase gene CvGPD1 in salt-tolerant yeast Candida versatilis in stimulated by high concentrations of NaCl. Yeast Watanabe Y, Takechi Y, Nagayama K, and Tamai Y Overexpression of Saccharomyces cerevisiae mannitol dehydrogenase gene (YEL w) in glycerol synthesisdeficient S. cerevisiae mutant. Enzyme Microbial. Technol. Watanabe Y, Tsuchimoto S, and Tamai YHeterologous expression of Zygosaccharomyces rouxii glycerol phosphate dehydrogenase gene ZrGPD1 and glycerol dehydrogenase gene ZrGCY1 in Saccharomyces cerevisiae. FEMS Yeast Res. Wong B, Murray JS, Castellanos M, and Croen KD D-arabitol metabolism in Candida albicans: Studies of the biosynthetic pathway and the gene that encodes NAD - dependent D-arabitol dehydrogenase. J. Bacteriol. Food Function
Introduction ur company has just started service to cut out sugar chains from protein and supply them to users by utilizing the handling technology of
Standard PA-Sugar Chain Catalogue Masuda Chemical Industries Co., LTD. http://www.mc-ind.co.jp Introduction ur company has just started service to cut out sugar chains from protein and supply them to users
More information™·”õ/sec3_p63_84/fiü“eflÅ
Section3 64 1 65 Section 3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 Babara E. Ainsworth et al. Med. Sci. Sports. Exevc.1993 66 67 Section 3 1 10 9 8 1 2 3 4 7 6 5 6 7 5 1 2 3 4 68 69 Section 3 2 70 Section 3
More information名称未設定-1
Study on Clinical Factors Affecting the Fungal Culture Test - Relevance of Dry Mouth - Yoshiko YAMAMURA, Yukihiro MOMOTA, Hideyuki TAKANO, Koichi KANI, Katsumi MOTEGI, Fumihiro MATSUMOTO, Masayuki AZUMA
More information19) Pearson, J., Southam, G. G. and Holley, R. A.: Survival and transport of bacteria in egg washwater. Appl. Environ. Microbiol., 53, 2060-2065 (1987). 20) Poovaiah, B. W.: Role of calcium in prolonging
More informationyakugaku-kot.ppt
2009 Masaaki Kotera kot@kuicr.kyoto-u.ac.jp 2 I II / A () B1 () B2 B12 C () D A D () () () () DNA 5- http://www.genome.jp/kegg/pathway.html KEGG PATHWAY Database Xenobiotics biodegradation http://www.genome.jp/kegg/pathway.html
More informationFig. 1. Structures of NM394, NAD-358 and NAD-245 Fig. 2. Typical HPLC chromatograms of NM394 in human plasma by organic solvent extraction method (a): Blank plasma (b): Plasma spiked with NM394 and internal
More information日本消化器外科学会雑誌第29巻第9号
Table 1 Oligonucleotide primers used for RT-PCR and internal probes used for Southern blot hybridization Cytokine Primer Sequence (5'-3') 5' 3' Internal probe s' 3' Internal probe 5' 3' Internal probe
More informationEPSON LP-8900ユーザーズガイド
3 4 5 6 7 8 abc ade w p s 9 10 s s 11 p 12 p 13 14 p s 15 p s A B 16 w 17 C p 18 D E F 19 p w G H 20 A B 21 C s p D 22 E s p w 23 w w s 24 p w s 25 w 26 p p 27 w p s 28 w p 29 w p s 30 p s 31 A s B 32
More informationJAMSTEC Rep. Res. Dev., Volume 12, March 2011, 27 _ 35 1,2* Pb 210 Pb 214 Pb MCA 210 Pb MCA MCA 210 Pb 214 Pb * 2
JAMSTEC Rep. Res. Dev., Volume 12, March 2011, 27 _ 35 1,2* 1 1 1 1 210 Pb 210 Pb 214 Pb MCA 210 Pb MCA MCA 210 Pb 214 Pb 2010 10 4 2010 12 10 1 2 * 237-0061 2-15 046-867-9794 ogurik@jamstec.go.jp 27 210
More information研究成果報告書(基金分)
N- GlcNAc β-1,4 GlcNAc GlcNAc DNA Neisseriaceae Chitiniphilus shinanonensis SAY3 T SAY3 GlcNAc C. shinanonensis SAY3 DNA SAY3 ORF pcold, pet GlcNAc HPLC pmo130 SAY3 SAY3 15 SAY3 DNA GC 67.6% 4.16 x 10
More information[Nippon Nogeikagaku Kaishi
[ Nippon Nageikagaku Kaishi Vol. 72, No. 11, pp. 1333 `1344, 1998 l Key words: methylotrophic yeast; gene expres sion; C1-metabolism; peroxisome Cellular Functions of the Methylotrophic Yeast: Their Molecular
More informationFig.1 Structural formulas of 6059-S(I) and decarboxylated product (11) Fig.2 Standard curve of 6059-S by agar well method Medium:Trypto-soy agar Diluent:0.1M Phosphate buffer,ph 7.0 Fig.3 Standard curves
More informationThe function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Database Center for Life Science Online Service Key words Shigey
The function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Key words Shigeyuki Kawai, Chikako Fukuda, Kousaku Murata E-mail : kawais@kais.kyoto-u.ac.jp, chikakko@kais.kyotou.ac.jp,
More information10中西_他.indd
Streptomyces mobaraensis N N N Sm b Sm N N Sm Sm Sm ' ' Sm Sm ak K k K bk k K Sm Sm a b S. mobaraensis a b a b Sm b b bb b b Sm Sm S. lividans ab Sm ab Sm ab ab ab ab SmSm S. lividansab S. lividans ab
More informationTox
CytoTox 96 Non-Radioactive Cytotoxicity Assay No. TB163J 2000 8 G1780 I. 2 II. 2 III. A. 4 B. CytoTox 96 Assay 4 IV. A. 5 B. 5 C. LDH 5 V. A. 7 B. 8 C. LDH 8 D. 9 VI. A. 11 B. 12 VII. 13 VIII. 14 IX. A.
More information1) AlP: a-naphthyl phosphate 10 mg, diazo blue B 20 mg Clark and Lub's buffer ph 9.2 2) AcP: a-naphthyl phosphate 10 mg, diazo 3) 0-Est: 0-naphthyl ac
1) AlP: a-naphthyl phosphate 10 mg, diazo blue B 20 mg Clark and Lub's buffer ph 9.2 2) AcP: a-naphthyl phosphate 10 mg, diazo 3) 0-Est: 0-naphthyl acetate 10 mg, diazo blue B 20 mg, Michaelis buffer Malic
More informationA, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et al., 1994; Shi et al., 1995 CTB C
2004 Analysis of the expression of cholera toxin B-subunit gene 1065010 2004 8 26 1 A, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et
More information304 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.0, -*. -*3 (,**1) 58 * ** *** : * : ** *** Development of Sorting System Based on Potato Starch C
304 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /., No.0, -*. -*3 (,**1) 58 * ** *** : * : ** *** Development of Sorting System Based on Potato Starch Content Using Visible and Near-Infrared Spectroscopy
More informationCHEMOTHERAPY APR Fig. 1 Chemical structure of cefotetan (CTT, YM09330)
CHEMOTHERAPY APR. 1982 Fig. 1 Chemical structure of cefotetan (CTT, YM09330) VOL.30 S-1 CHEMOTHERAPY Fig. 2 Comparison of standard curves of CTT on various test organisms by cylinder plate method Column
More information988 CHEMOTHERAPY NOV. 1971
988 CHEMOTHERAPY NOV. 1971 VOL. 19 NO. 8 CHEMOTHERAPY 989 Effect of medium-ph and inoculum size on activity of SB-PC heart infusion agar, mcg/ml Sensitivity distribution of Staphylococci to SB-PC in surgical
More informationCandida albicans In Vitro Diagnostics (13)--D-Glucan Determination Reagents
D 012 Candida albicans In Vitro Diagnostics (13)--D-Glucan Determination Reagents DATA : 1 DATA : 2 3 DATA : UNGITEC 1 1 DATA : 20pg/mL 1020pg/mL 10pg/mL (cut off 20pg/mL) 90.2%(37/41) 100%(85/85) 96.8%(122/126)
More informationAqua Way Philic Aqua Way Cation Technology platform Cell-sheet engineering (1) Technology platform Cell-sheet engineering (2) 1) Collection of cells in a form of thin film 2) o suture required for transplantation
More information(Quality of Life; QL) QL (Nerve Growth Factor; NGF) NGF PC12 6- (6-HITC) NGF NGF NGF PTP1B 6-HITC PTP1B NGF PTP1B 6-HITC PTP1B 6-HITC NGF 6-HITC 6-HITC in vivo 464-8601 Tel: 052-789-4126 20-Hydroxyecdysone
More information秋田応用微生物研究会
13:00-13:10 13:10-13:30 13:30-13:50 (Tricholoma auratum) 13:50-14:10 14:10-14:30 14:30-14:50 14:50-15:10 α-msh 15:10-15:30 Lewis 15:30-15:50 15:50-16:10 16:10-16:30 (1) (2) ( ) (2) (1) (2) (3) (1) (2)
More information研究成果報告書
Trichoderma reesei Cel3A Cel1A Cel3C (BGL) BGL Cel3A Cel3C pnp-glc Cel1A BGL Cel1A BGL BGL Cel3A BGL Trichoderma reesei, which is well known to produce large amount of cellulases, expresses three β-glucosidases
More information25 6 7 X (crystallizing ligand) Fv Fv 3.5 Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound
More information1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (
1. Precise Determination of BaAl2O4 Cell and Certification of the Formation of Iron Bearing Solid Solution. By Hiroshi UCHIKAWA and Koichi TSUKIYAMA (Central Research Laboratory, Onoda Cement Co., Ltd.,
More informationContinuous Cooling Transformation Diagrams for Welding of Mn-Si Type 2H Steels. Harujiro Sekiguchi and Michio Inagaki Synopsis: The authors performed
Continuous Cooling Transformation Diagrams for Welding of Mn-Si Type 2H Steels. Harujiro Sekiguchi and Michio Inagaki Synopsis: The authors performed a series of researches on continuous cooling transformation
More informationJ53-01
53 Bulletin of Hiroshima Jogakuin University : 126Dec. 2003 1 TAT 200310 Object relations in TAT analysis Kyoko YAMASHITA Abstract I carried out TAT with 54 female university students as subjects, and
More informationFA
29 28 15 1985 1993 The process of the labor negotiations of the Japan Professional Baseball Players Association, 1985 1993 ABE Takeru Graduate School of Social Science, Hitotsubashi University Abstract
More information内科96巻3号★/NAI3‐1(第22回試験問題)
µ µ α µ µ µ µ µ µ β β α γ µ Enterococcus faecalis Escherichia coli Legionella pneumophila Pseudomonas aeruginosa Streptococcus viridans α β 正解表正解記号問題 No. 正解記号問題 No. e(4.5) 26 e 1 a(1.2) 27 a 2
More informationuntitled
RYZA IL & FAT CHEMICAL C., LTD. SAKURA EXTRACT Ver.1.4MK Ver. 1.4MK 1) 1300 1929 1 Ver. 1.4MK Sakura 1-caffeoyl---D-glucopyranoside quercetin 3---D-glucopyranoside 1) :, 4, 11-22 (1995). 2 Ver. 1.4MK 3
More information474 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No.3,.1..2* (,**0) 24 Measurement of Deterioration of Frying Oil Using Electrical Properties Yoshio
474 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. / No.3.1..2 (0) 24 Measurement of Deterioration of Frying Oil Using Electrical Properties Yoshio Hagura Yoshihiro Sasaki and Kanichi Suzuki Graduate School
More informationCHEMOTHERAPY JUN Citrobacter freundii 27, Enterobacter aerogenes 26, Enterobacter cloacae 27, Proteus rettgeri 7, Proteus inconstans 20, Proteus
VOL. 32 S-4 CHEMOTHERAPY Fig. 1 Chemical structure of sodium cefoperazone Fig. 2 Chemical structure of sodium cefoperazone CHEMOTHERAPY JUN. 1984 Citrobacter freundii 27, Enterobacter aerogenes 26, Enterobacter
More informationHILIC UPLC/MS UPLC LC/ MS LC/MS HPLC 2-4 HPLC 4,5 HILIC / HILIC 80 ACQUITY UPLC Xevo QTof MS ACQUITY BEH HILIC HILIC TOF ESI 1.7 µm BEH HILIC UPLC HIL
HILIC UPLC/MS UPLC LC/ MS LC/MS HPLC 2-4 HPLC 4,5 HILIC / HILIC 80 ACQUITY UPLC Xevo QTof MS ACQUITY BEH HILIC HILIC TOF ESI 1.7 µm BEH HILIC UPLC HILIC UPLC HPLC 1 100 µl 900 µl 15 900 µl 500 µl 470 µl
More information5) Kawada T, Hagihara K, Iwai K (1986) Effects of capsaicin on lipid metabolism in rats fed a high fat diet. J Nutr 116 : 1272-8. 6) Kawada T, Watanabe T, Takaishi T, Tanaka T, Iwai K (1986) Capsaicin-induced
More information9 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.0,,/+,/0 (,**/) 251 * * E#ects of Microbial Transglutaminase on Melting Point and Gel property of G
9 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.0,,/+,/0 (,**/) 251 * * E#ects of Microbial Transglutaminase on Melting Point and Gel property of Gelatins Takahiko Soeda, Tomoko Kaneko*, Akiko Hokazono,
More information1272 CHEMOTHERAPY MAR. 1975
1272 CHEMOTHERAPY MAR. 1975 VOL. 23 NO. 3 CHEMOTHERAPY 1273 Fig. 2 Minimal inhibitory concentration of aminoglycosides against 50 strains of Klebsiella Fig. 1 Minimal inhibitory concentration of aminoglycosides
More information色覚(初組4).pm
733 3 1 520 1 8 4 0.2500 1 30012 1 AB 3 22 3 1 2 http://www.nig.ac.jp/labs/devgen/mou.html 734 1.1 360nm 830nm 380nm 780nm 540nm 580nm 660nm 540nm 660nm Log relative corneal sensitivity 0 1 2 400 500 600
More informationStudies on the Antigens Expressed on Trophoblasts Related to MLR-blocking Antibodies Shigenori GOTO Department of Obstetrics and Gynecology, Niigata University School of Medicine (Director: Prof. Shoshichi
More informationExtraction of Trehalose from Saccharomyces cerevisiae by High-Pressure Treatment Miwako KINEFucHI,* Akira YAMAZAKI* and Kouzi YAMAMOTO** * Research Institute, Echigo Seika Co., Ltd. (1-4-5, Gofukumachi,
More informationuntitled
2010 12 12 2 RITE 1 C6 C5 RITE Sustainable Industry C2 C3 C4 2 1 take-off food non-food DuPont C 6 2013 Advanced fuels 3 in ( kl/y) POET Broin 2011 2011 C5C6 9.5 Novozymes Z. mobilis Vercipia (Verenium/BP)
More informationヒト血漿中オキシステロールの高感度分析法
High sensitive determination of oxysterols in human plasma using gas chromatography-mass spectrometry (GC-MS) Saori Nakagawa and Susumu Yamato Oxysterols, cholesterol oxidation products, have been important
More information448 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.+*,..2./- (,**/) 14 * * The E#ect of Peptides on the Physical Properties of Soy Protein Gel Takah
448 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /,, No.+*,..2./- (,**/) 14 * * The E#ect of Peptides on the Physical Properties of Soy Protein Gel Takahiko Soeda, Akiko Hokazono and Katutoshi Yamazaki* Department
More informationStudy on Application of the cos a Method to Neutron Stress Measurement Toshihiko SASAKI*3 and Yukio HIROSE Department of Materials Science and Enginee
Study on Application of the cos a Method to Neutron Stress Measurement Toshihiko SASAKI*3 and Yukio HIROSE Department of Materials Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa-shi,
More information<Original Papers> Genetic instability of Acetobacter pasteurianus revealed by whole genome analyses
Mem. Faculty. B. 0. S. T. Kinki University No. 27 : 29 38 (2011) Jatropha curcas L Jatropha integerrima (1) K.Y. Santosh, A.J. Asha, K. G.Phani, K. Phani, R.T. Prashant, K.S. Sanjeev, Singh, and
More information論文
*, 1 1 Selection of superior Frankia spp. for rapid cultivation of Alnus hirsuta seedlings Maki Saito *, 1 and Mikio Hasegawa 1 4 2 4 28 9 30 cm 30 cm 70 % 5 Abstract: In order to develop the rapid cultivation
More informationv 1 v 2 e g ˆ Š Œ Ž p š ~ m n u { i 1, i 2, i 3, i 4 } { i 1, i 5 } v 1 v 2 v 3 v 4 v 5 v 6 { i 1, i 2, i 4 } { i 1, i 2, i 3, i 5 } { i 1, i 3, i 4 }
DEIM Forum 2009 D2-1 COPINE: 112 86 2 1 1 E-mail: {seki,sesejun}@sel.is.ocha.ac.jp COPINE COPINE: Mining Networks Sharing Common Patterns Mio SEKI and Jun SESE Graduate School of Humanities and Sciences,
More informationA STUDY ON GENITAL SKIN ANDROGEN RECEPTOR BY DISPERSED CELL METHOD Yasukazu Sen and Masaaki Morioka Department of Urology, Kochi Medical School, Nankoku, Japan (Director: Prof. Yukitoshi Fujita) Yoshiteru
More information会報35号表紙.pdf
35 No. (2017 1 ) RNA 2017 2017 TV 365 365 chunky 26 RNA 2017 Congratulations26 30-1 - RNA 2017-2 - - 3 - - 4 - - 5 - - 6 - 2016 8 31 9 2 RNA 2016 1 RNA RNA RNA RNA RNA 1-7 - RNA RNA 3 3 7 31 RNA ncrna
More information【教】⑥久我直人先生【本文】/【教】⑥久我直人先生【本文】
Schön Schön Shulman Wilson transformation pedagogical content knowledge Schön Schön Schön Shulman Wilson pedagogical content knowledge Schön Schön Shulman Wilson pedagogical content knowledge Schön Shulman
More informationKishu saba-narezushi narezushi Spinacia oleracea
Kishu saba-narezushi narezushi Spinacia oleracea Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1 1 1 Sakuragaoka, Setagaya-ku, Tokyo 156 8502
More informationuntitled
1 1 2 CADLIVE E-CELL 3 GEM CE/MS MGF WT MGF 4 2 WT E-Cell MASK 5 CE-MS, CE- TOFMS LC-MS 24, 5 9 2 0 6 3 CE-MS CE MS Soga, T. et al. (2002) Anal Chem. 74, 2233-2239 7 CE-MS 859 452, 269, 86 8 4 LC-MS 9
More informationJ. Soc. Cosmet. Chem. Jpn. 7-chome, Edogawa-ku, Tokyo 132, Japan 2.1 J. Soc. Cosmet. Chem. Japan. Vol. 31, No
J. Soc. Cosmet. Chem. Jpn. 7-chome, Edogawa-ku, Tokyo 132, Japan 2.1 J. Soc. Cosmet. Chem. Japan. Vol. 31, No. 2 1997 167 Fig.-1 Balanced fiber method Fig.-2 The effects of perming and IG on breaking off
More information社会学部紀要 118号☆/6.藤原
March 4 3 989 98 988 0 990 03 9 9 97 3 98 988 9890 03 0 9 97 97 2 4 4 4 2 9 69 9 97 74972 68 8 9 96 97 98 99 960 March 4 96 962 D 963 964 96 8 2 966 8 if MASH 967 968 0 97 972 969 970 March 4 973 974 97
More informationWeb Web Web Web Web, i
22 Web Research of a Web search support system based on individual sensitivity 1135117 2011 2 14 Web Web Web Web Web, i Abstract Research of a Web search support system based on individual sensitivity
More informationb) Gram-negative bacteria Fig. 2 Sensitivity distribution of clinical isolates : E. coli Fig. 3 Sensitivity distribution of clinical isolates : Pseudomonas Fig. 1 Sensitivity distribution of clinical isolates
More information16_.....E...._.I.v2006
55 1 18 Bull. Nara Univ. Educ., Vol. 55, No.1 (Cult. & Soc.), 2006 165 2002 * 18 Collaboration Between a School Athletic Club and a Community Sports Club A Case Study of SOLESTRELLA NARA 2002 Rie TAKAMURA
More informationδδ 1 2 δ δ δ δ μ H 2.1 C 2.5 N 3.0 O 3.5 Cl 3.0 S μ
1961. (Received January 15, 2013) 1) 1. 2. ph 2) 3) 2) 2) 2001 4 6) 7) ph ph ph 1961 1962 1. 2 1 1 δδ 1 2 δ δ δ δ μ 1 1 1 1. H 2.1 C 2.5 N 3.0 O 3.5 Cl 3.0 S 2.5 1 1. μ 1 3. 1 2. 1963 1,4- δ δ 1 3 2 δ
More informationFig.1 Chemical structure of BAY o 9867
Fig.1 Chemical structure of BAY o 9867 CHEMOTHERAPY 43 Table 3 Antibacterial spectrum of gram negative bacteria Medium:Heart infusion agar (Nissui) Method:Agar dilution (Streak) CHEMOTHERAPY DEC 1985
More informationCHEMOTHERAPY JUNE 1987 Table1 Media used *BHIB, brain heart infusion broth (Difco); /3 -NAD, S -nicotinamidoadeninedinucleotide (Sigma Chemical Co.);
VOL.35 S-2 CHEMOTHERAPY Fig.1 Chemical structure of carumonam CHEMOTHERAPY JUNE 1987 Table1 Media used *BHIB, brain heart infusion broth (Difco); /3 -NAD, S -nicotinamidoadeninedinucleotide (Sigma Chemical
More informationuntitled
Tuber indicum Tuber indicum 20 -1- 30cm Ganoderma lucidum ( ) : (1982) Ganoderma japonicum Ganoderma lucidum Ganoderma neo-japonicumu 30cm : Ganoderma lucidum (1977) -2- -3- -4- -5- -6- -7- -8- -9- - -
More information;~ (Summary) The Study on the Effects of Foot Bathing on Urination Kumiko Toyoda School of Human Nursing, University of Shiga Prefecture Background Foot bathing is one of the important nursing care for
More informationuntitled
21 19 12 16 16 13 107 19 21 108 80ha 40 24 109 13 18 10 20 110 111 16 24 112 113 58 63 11 14 14 16 19 16 17 17 21 21 114 3.1 126 3.1 / 126 / 2.8 / 109 / 40 4445 550 360 305 18 18 19 115 200 45 10 116 117
More informationEVALUATION OF NOCTURNAL PENILE TUMESCENCE (NPT) IN THE DIFFERENTIAL DIAGNOSIS OF IMPOTENCE Masaharu Aoki, Yoshiaki Kumamoto, Kazutomi Mohri and Kazunori Ohno Department of Urology, Sapporo Medical College
More informationuntitled
Vol. 1 (2005) pp. 91-103 * ( 17 1 27 ) Approach from Bioelectronics to Bio-Interface Masashi YAOITA * Our studies for approach to bio-interface were fabrication of a needle type glucose sensor for continuous
More informationABSTRACT The Social Function of Boys' Secondary Schools in Modern Japan: From the Perspectives of Repeating and Withdrawal TERASAKI, Satomi (Graduate School, Ochanomizu University) 1-4-29-13-212, Miyamaedaira,
More informationKey words: bacterial meningitis, Haemophilus influenzae type b, Streptococcus pneumoniae, rapid diagnosis, childhood
Key words: bacterial meningitis, Haemophilus influenzae type b, Streptococcus pneumoniae, rapid diagnosis, childhood Fig.1 Distribution of the cases with bacterial meningitis by age and pathogens Chiba
More information00-Ł\”ƒ
Effect of Organic Substrates on the Transportability and Growth of Nursery Stock of Satsuma Mandarin cv. Isiji Katsuhiko NAKAMOTO, Naohisa MIYAWAKI and Mihoko HASEGAWA Reprinted from Bulletion of the Hiroshima
More information橡最終原稿.PDF
GIS Simulation analysis of disseminate of disaster information using GIS * ** *** Toshitaka KATADAJunsaku ASADA and Noriyuki KUWASAWA GIS GIS AbstractWe have developed the simulation model expressing the
More informationスポーツ選手における日常的トレーニングが味覚に及ぼす影響について
Study on the Type Partition of Hospital Meals in Japan. -Relationship between Foods and Cooking Techniques- Tomoko WATANABE *1, Kunio ISHII *1, Sachiko SUGIZAKI *1, Kanako MURAMATSU *1, Megumi HIGUCHI
More informationCHEMOTHERAPY Proteus mirabilis GN-79 Escherichia coli No. 35 Proteus vulgaris GN-76 Pseudomonas aeruginosa No. 11 Escherichia coli ML-1410 RGN-823 Kle
VOL. 29 NO.8 CHEMOTHERAPY 865 CHEMOTHERAPY Proteus mirabilis GN-79 Escherichia coli No. 35 Proteus vulgaris GN-76 Pseudomonas aeruginosa No. 11 Escherichia coli ML-1410 RGN-823 Klebsiella pneumoniae GN-69
More information三木研授業2009.key
( ) 1980 1.54A 1.54A ( ) 1A 0.98A 2.29A ( ) 2dsinθ = λ (2d hkl sinθ hkl = λ) ()? Bragg 2dsinθ = λ 2θ 1 d sinθ = λ 2d Bragg 2dsinθ = λ? Bragg? 2dsinθ = λ? λ 2 I t 3 λ 2 exp( µt) UW Arndt, J. Appl. Cryst.
More informationX線分析の進歩36 別刷
X X X-Ray Fluorescence Analysis on Environmental Standard Reference Materials with a Dry Battery X-Ray Generator Hideshi ISHII, Hiroya MIYAUCHI, Tadashi HIOKI and Jun KAWAI Copyright The Discussion Group
More information日本化学療法学会雑誌第57巻第1号
In vitro Streptococcus pneumoniae Escherichia coli in vitro Streptococcus pneumoniae Escherichia coli µs. pneumoniae E. coli µ Key words in vitrostreptococcus pneumoniae Escherichia coli Escherichia coli
More information.N..
Examination of the lecture by the questionnaire of class evaluation -Analysis and proposal of result at the first term of fiscal year - Kazuo MORI, Tukasa FUKUSHIMA, Michio TAKEUCHI, Norihiro UMEDA, Katuya
More informationCHEMOTHERAPY Fig. 1 Chemical structure of TE-031
CHEMOTHERAPY Fig. 1 Chemical structure of TE-031 CHEMOTHERAPY Table 1 Experimental groups for active systemic anaphylaxis in guinea pigs : Single dosage Fig. 2 Schedule for active systemic anaphylaxis
More informationGID1, Hiroaki KATO, Tomomi SATO and Miyako UEGUCHI-TANAKA: Gibberellin Receptor GID1: Gibberellin Recognition and Molecular Evolution
52 31-36 2010 2 GID1, Hiroaki KATO, Tomomi SATO and Miyako UEGUCHI-TANAKA: Gibberellin Receptor GID1: Gibberellin Recognition and Molecular Evolution Gibberellins GAs are phytohormones essential for many
More informationCHEMOTHERAPY Fig. 1 Body weight changes of pregnant mice treated orally with AM- 715 Day of sestation
CHEMOTHERAPY CHEMOTHERAPY Fig. 1 Body weight changes of pregnant mice treated orally with AM- 715 Day of sestation CHEMOTHERAPY Table 1 Preliminaly test of AM- 715 1): Mean } SD *: Significant difference
More informationJan THE JAPANESE JOURNAL OF ANTIBIOTICS XL-1 Table 1. Outline of administering doses, routes and sampling times *: 4 ml/hr/kg Bacillus subtilis
THE JAPANESE JOURNAL OF ANTIBIOTICS XL-1 Jan. 1987 Jan. 1987 THE JAPANESE JOURNAL OF ANTIBIOTICS XL-1 Table 1. Outline of administering doses, routes and sampling times *: 4 ml/hr/kg Bacillus subtilis
More information駒田朋子.indd
2 2 44 6 6 6 6 2006 p. 5 2009 p. 6 49 12 2006 p. 6 2009 p. 9 2009 p. 6 2006 pp. 12 20 2005 2005 2 3 2005 An Integrated Approach to Intermediate Japanese 13 12 10 2005 8 p. 23 2005 2 50 p. 157 2 3 1 2010
More information,,.,.,,.,.,.,.,,.,..,,,, i
22 A person recognition using color information 1110372 2011 2 13 ,,.,.,,.,.,.,.,,.,..,,,, i Abstract A person recognition using color information Tatsumo HOJI Recently, for the purpose of collection of
More information渡辺(2309)_渡辺(2309)
[ 29 p. 241-247 (2011)] ** *** ** ** Development of a nickel-based filler metal containing a small amount of silicon by WATANABE Takehiko, WAKATSUKI Ken, YANAGISAWA Atsusi and SASAKI Tomohiro Authors tried
More information後期化学_01_濃度
2011 ( ) 1 4 1 100 g g % * 1 100 g 1 g 1 % * 2 (1 g)/(100 g) = 0.01 = 1 % 100 g 100 g 99 g 100 g 1 g 1 % 2.5 g 50 g (2.5 g)/(50 g) = 0.050 = 5.0 % 100 ml g 1 %(w/w) wt% % %(w/v) % / 2 % SI 2 / % 100 ml
More informationTitle 歯性病巣の関連する皮膚疾患におけるビオチンの効用 Author(s) 高橋, 愼一 ; 川島, 淳子 ; 森本, 光明 ; 山根, 源之 Journal, (): - URL Right Posted at the Inst
Title 歯性病巣の関連する皮膚疾患におけるビオチンの効用 Author(s) 高橋, 愼一 ; 川島, 淳子 ; 森本, 光明 ; 山根, 源之 Journal, (): - URL http://hdl.handle.net/10130/526 Right Posted at the Institutional Resources for Unique Colle Available from
More information技術研究報告第26号
1) 2) 3) 250Hz 500Hz RESEARCH ON THE PHYSICAL VOLUME OF THE DYNAMIC VIBRATION RESPONSE AND THE REDUCTION OF THE FLOOR IMPACT SOUND LEVEL IN FLOORS OF RESIDENTIAL HOUSING Hideo WATANABE *1 This study was
More informationCHEMOTHERAPY aureus 0.10, Enterococcus faecalis 3.13, Escherichia coli 0.20, Klebsiella pneumoniae, Enterobacter spp., Serratia marcescens 0.78, Prote
aureus 0.10, Enterococcus faecalis 3.13, Escherichia coli 0.20, Klebsiella pneumoniae, Enterobacter spp., Serratia marcescens 0.78, Proteus mirabilis 3.13, Proteus vulgaris 1.56, Citrobacter freundii 0.39,
More informationJpn.J.Leprosy 67, (1998)
Jpn.J.Leprosy 67,287-291(1998) Fig.1. Macroscopic appearance of the cultured slides stained by the Ziehl-Neelsen method. Up to bottom: kept at 4 C, 18 weeks, cultured for 6 weeks, 8 weeks, 10 weeks, 12
More information1_alignment.ppt
" " " " n " n n " n " n n n " n n n n " n LGPSSKQTGKGW-SRIWDN! + +! LN-ITKSAGKGAIMRLGDA! " n -------TGKG--------!! -------AGKG--------! " n w w w " n w w " " " 11 12 " n w w w " n w w A! M! O! A!
More information1.WizardPlusSV日本語プロトコール
Wizard Plus SV Minipreps DNA Purification System No. TB281J 2001 7 A1270, A1330, A1340, A1460 A1470 Wizard Plus SV Minipreps DNA Purification System (a) DNA 30 20,000bp DNA Recombinant RNasin Ribonuclease
More informationR R S K K S K S K S K S K S Study of Samuhara Belief : Transformation from Protection against Injuries to Protection against Bullets WATANABE Kazuhiro Samuhara, which is a group of letters like unfamiliar
More information1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The Boston Public Schools system, BPS (Deferred Acceptance system, DA) (Top Trading Cycles system, TTC) cf. [13] [
Vol.2, No.x, April 2015, pp.xx-xx ISSN xxxx-xxxx 2015 4 30 2015 5 25 253-8550 1100 Tel 0467-53-2111( ) Fax 0467-54-3734 http://www.bunkyo.ac.jp/faculty/business/ 1 [1, 2, 3, 4, 5, 8, 9, 10, 12, 15] The
More information1: DTS r 1, r 2 v ρ(x) = π(r1 2 r2) 2 dr dt 1 v x (2) t=x/v DTS [2] wt% KCl %/ 2 3 5wt% NaCl 3wt% ( ) 2 45 NaCl 300Hz 4-1.3%/ [2]
5 2011 12 14 Distributed temperature sensor (DTS) technology is used widespreadly among many applications, such as temperature monitoring in plant engineering. The author has developped a novel DTS, capable
More informationKey words: E. coli O 157: H7, fosfomycin, verotoxin, mouse infection
Key words: E. coli O 157: H7, fosfomycin, verotoxin, mouse infection Table 1. Bacterial cell counts in feces of mice infected with Esclwrichia coli O 157: H7 NK2 before and during oral dosing with fosfomycin
More information酵素化学
1 2 3 DNA RNA 4 5 6 7 8 9 H 2 O 10 http://www.ultranet.com/~jkimball/biologypages/c/carbohydrates.html#starches http://www.wantashi.com/aroma/refre/page12.html 11 mono : di : 2 tri : 3 poly : 12 13 14
More informationLAGUNA LAGUNA 8 p Saline wedge at River Gonokawa, Shimane Pref., Japan Saline water intrusion at estuary r
LAGUNA8 67 78 2001 3 LAGUNA 8 p.67 78 2001 1 1 2 3 4 5 6 7 8 Saline wedge at River Gonokawa, Shimane Pref., Japan Saline water intrusion at estuary river and its relation to the underground water Observation
More information*.E....... 139--161 (..).R
A Preliminary Study of Internationalization at the Local Level: The Case of Aikawa Town in Kanagawa Prefecture, Japan FUKUSHIMA Tomoko and FUJISHIRO Masahito In recent years, as foreign residents increase
More information1) Asa, S. L., et al.: Immunohistochemical localization of neuron-specific enolase in the human hypophysis and pituitary adenoma, Arch. Pathol. Lab. Med., 108: 40 `43, 1984. 2) Chi, B. H. and Kim, R. C.:
More informationPurification and Properties of Acid Pyrophosphatase from the Potato Yasuko TANEMURA, Hiroshi WADA*, Takashi ITO*, Haruhito TSUGE* and Kazuji OHASHI* D
Purification and Properties of Acid Pyrophosphatase from the Potato Yasuko TANEMURA, Hiroshi WADA*, Takashi ITO*, Haruhito TSUGE* and Kazuji OHASHI* Department of Home Economics, Shotoku Gakuen Women's
More information