0315_StemCell_J.indd
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- まれあ さわまつ
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1 STEM CELL PORTFOLIO driving breakthroughs in research through innovations in process
2 10 Go to lifetechnologies.com/stemcell 2
3 Contents ECM Culture Differentiate Engineer Analyze 3
4 KnockOut Serum Replacement (SR) ESC/iPSC KnockOut SR300 ESC/iPSC KnockOut SR ESC/iPSCFBS KnockOut SRKnockOut DMEM KnockOut DMEM/F-12 1 FBS Go to KnockOut SR XenoFree CTS ESC/iPSC xeno-free KnockOut SR XenoFreeKnockOut SRhESC/hiPSC xeno-freehesc CELLstart CTS KnockOut SR GF Cocktail 2 Go to 1. ESC/iPSC 100 DMEM Culture system Basal media Complete media Supplements Feeders and extracellular matrices KnockOut DMEM Human ESC/iPSC ESC ESC FBS KnockOut Serum Replacement Serum + feeders Serum-free + feeders Serum-free + feeder-free Xeno-free + feeders (human) Xeno-free + feeder-free Mouse ESC/iPSC KnockOut DMEM KnockOut DMEM/F-12 KnockOut DMEM KnockOut DMEM/F-12 KnockOut DMEM KnockOut DMEM StemPro hesc SFM ESC-Qualified FBS KnockOut SR KnockOut SR XenoFree KnockOut SR XenoFree + KnockOut SR Growth Factor Cocktail Mouse embryonic fibroblasts Mouse embryonic fibroblasts Geltrex hesc-qualified/ CELLstart CTS Human foreskin fibroblasts CELLstart CTS CELLstart CTS 1. ESC ESC FBS KnockOut SR DMEM KnockOut DMEM D3 ESC LIF 7 ESC Serum + feeders Serum-free + feeders KnockOut DMEM DMEM/F-12 KnockOut DMEM ESC-Qualified FBS KnockOut SR Mouse embryonic fibroblasts Mouse embryonic fibroblasts 4
5 KnockOut SR Growth Factor Cocktail ESC/iPSC KnockOut SR Growth Factor Cocktail GFC ESC/iPSC ESC/iPSC2 KnockOut SR KnockOut SR XenoFree CTS ESC/iPSC KnockOut DMEM ESC/iPSC KnockOut DMEM ESC ipsc DMEM ESC/iPSCKnockOut SR KnockOut SR XenoFree CTS ESC FBS L-L- GlutaMAX Go to KnockOut DMEM/F-12 ESC/iPSC KnockOut DMEM/F-12 DMEM/F-12ESC ipsc ESC/iPSC hesc L- KnockOut SR MEF Kit and Gibco Mouse Embryonic Fibroblasts (MEFs) ESC/iPSC Gibco Mouse Embryonic Fibroblast MEF KnockOut Serum Replacement ICR 13.5 E13.5 2P2 80% Go to A B C 2. KnockOut SR Growth Factor Cocktail ips ipscgeltrex 20% KnockOut SR KnockOut SR Growth Factor Cocktail 2 mg/mla 40B DAPI 100C SSEA4 100 KnockOut SR XenoFree CELLstart CTS xeno-free substrate KnockOut SR GF Cocktail ipsc ipsc 5 5
6 StemPro hesc SFM hesc/hipsc StemPro hesc SFMhESC/hiPSC 3StemPro hesc SFMGeltrex RGF Matrix Geltrex hesc-qualified CELLstart CTS hesc/ipsc Go to MesenPRO RS Medium MSC2% MesenPRO RS DMEM + 10% FBS MSC 2% FBS10 20% FBS Go to StemPro MSC Serum Free Medium (SFM) MSC StemPro MSC SFM MSC DMEM + 10% FBS p63 CFU-F 6 MSC Go to StemPro MSC SFM XenoFree MSC xeno-free StemPro MSC SFM XenoFree MSC ADSCDMEM + 10% MSC FBSStemPro MSC SFM XenoFree CELLstart Substrate 6 MSC Go to 3. StemPro hesc SFM hesc StemPro hesc SFMhESC3 StemPro hesc SFM25 BG02 6
7 StemPro LipoMAX Supplement xeno-free StemPro LipoMAX StemPro LipoMAX ADSC 3 50% MSCADSC ADSC Go to Synth-a-Freeze Medium Synth-a-Freeze 10%DMSO Synth-a-Freeze 1 MSC ESC ipsc Synth-a-Freeze StemPro NSC SFM StemPro NSC SFMhNSC 4 NSC NSC StemPro NSC SFMNSC Go to Neurobasal and Neurobasal -A Media Neurobasal Medium Neurobasal -A Medium Go to StemPro -34 SFM CD34+ SFM StemPro -34 SFM StemPro -34 SFM Go to 4. StemPro NSC SFM 7
8 StemPro EZPassage Tool StemPro EZPassage 1 hesc/hipsc Go to to watch the StemPro EZPassage Tool video. 5. StemPro EZPassage hesc/hipscstempro EZPassage Gibco TrypLE Select CTS Invitrogen TrypLE Select CTS Go to StemPro Accutase StemPro Accutase heschnsc 6 hesc Go to A B 6. StemPro Accutase 1 ml 10 A B 8
9 Alexa 594-A Alexa 594-A Dynabeads SSEA-4 Dynabeads SSEA-4SSEA-4+ ESC 7 hesc Go to Dynal CD34 Progenitor Cell Selection System CD34+ CD34+ DC Dynabeads in vitro Dynabeads ISO 9001:2008 Dynabeads CTS Class 100 Dynabeads cgmp Dynabeads CTS ISO 13485:2003 Dynabeads Dynabeads Invitrogen Dynal Dynabeads ISO 9001:2008 ISO 13485: CFR 820 US cgmp DynaMag CTS Dynabeads Dynabeads CD3/CD28 CTS T FSC-A (x 1,000) FSC-A (x 1,000) 7. hesc BG01 Dynabeads SSEA-4 SSEA-4 9
10 in vitro HDFa HDFn HEKa HEKn HEMa HEMn Go to Gibco Human Neural Stem Cells (H9-Derived) StemPro NSC SFM StemPro Human Adipose-Derived Stem Cell Kit ADSC ADSC ADSC MesenPRO RS Gibco Rat Fetal Neural Stem Cells NSC 75% Gibco Mouse (C57BL 6) Mesenchymal Stem Cells 8C57BL/6 Gibco Rat (SD) Mesenchymal Stem Cells 8Sprague-Dawley SD StemPro Rat Alk Phos Expressing Mesenchymal Stem Cells ROSA26 Fisher 3448 Go to 8. ELF 97 Endogenous Phosphatase Kit StemPro Rat Alk Phos Expressing Mesenchymal Stem Cell 10
11 Geltrex hesc-qualified Geltrex hesc-qualifiedstempro hesc SFM hescpcr Geltrex hesc-qualified hesc/hipsc CELLstart CTS xeno-free CELLstart CTS xeno-free CELLstart CTS hesc/hipschmschnsc 9 hesc/hipsc Children s Memorial Research Center E. Seftor CELLstart CTS StemPro hesc SFMhESCH Go to Natural Mouse Laminin A B 9. MSCCELLstart CTS 4 4 MSC CELLstart CTS StemPro MSC SFM XenoFree 9 B A 11
12 StemPro Accutase TrypLE Select CTS TrypLE Express StemPro Accutase TrypLE TrypLE ESC ipsc NSC TrypLE Select CTS TrypLE Express TrypLE Select CTS PSC MSC NSC HSC HSC 100 ml 100 ml 100 ml 500 ml 5 L 12
13 Dispase Collagenase IV StemPro EZPassage ESC ipsc Clostridium histolyticumpro-x-glyc- ProX StemPro EZPassage ESC ipsc 5 g 1 g 10 13
14 ECM 1998ESC Gibco Gibco ECMin vitro Laminin Fibronectin Collagen Geltrex Geltrex Engelbreth-Holm-Swarm ECM EHS Engelbreth-Holm- hesc/hipsc Swarm EHS Matrigel ESC ipsc Geltrex NSC PSC MSC NSC HSC 1 mg I 10 ml I 20 ml24 hesc LDEV 14
15 CELLstart CTS AlgiMatrix Gelatin Vitronectin CELLstart CTS AlgiMatrix xeno- 3 free CELLstart CTS 0.1% Recombinant Vitronectin hesc hipsc hmsc 1 Natural Human hnsc MEF AlgiMatrix MEF 2 ml ml 100 mg 15
16 Gibco Gibco Gibco FGF-basic Full Length CTS LIF BMP-4 SCF CTS BDNF EPO IL-2 IL-4 IL-7 CTS TNF- CTS Go to B-27 Supplement B-27 Supplement50 Gibco B-27 Supplement xeno-free N-2 Supplement N-2 SupplementPNS CNS G-5 Supplement G-5 Supplement Neurobasal Go to Gibco MSC StemPro MSC SFM MesenPRO RS MSC FBS cgmp PCR StemPro Adipogenesis Differentiation Kit 10 StemPro Chondrogenesis Differentiation Kit StemPro Osteogenesis Differentiation Kit Go to O 16
17 Gibco Gibco Cell Therapy Systems (CTS ) Gibco Cell Therapy Systems CTS 21 CFR Part 820 Quality System RegulationISO 13485/ISO 9001 Gibco Gibco 2 cgmp cgmpgibco Cell Culture Custom Media Services10,000 L 1,000 L Advanced Granulation Technology AGT 7,500 kg Gibco MediaExpress SM cgmp Gibco cgmp SDS-PAGE >95% SDS-PAGE ED 50 PD-Direct PD-Direct Bioprocess Services LAL Limulus Amoebocyte Lysate <0.1 g/mg 2. Gibco Gibco cgmp cgmp Gibco I21 CFR Part 864, subpart CcGMP cgmp Custom Products Services 17
18 Jump-In TI expression Jump-In TI Gateway R4 attp GOI 1 20 GPCR Go to Gateway Cloning Gateway Gateway 1 99% 1 DNA Go to Jump-In TI Gateway 21R4 attpjump-in TI 2R4R4 attp >95% 11 R4 attp Zeocin SV40 pa PhiC31 attb Ampicilin UMS HSV TK pjti /Zeo 6597 bp Hygromycin Phi C31 Integrase R4 att P Step I UMS Zeo TK-Hyg puc ori Amp puc ori HSV TKPA Amplicilin attr2 ccdb Cm R attr1 puc ori pjti R4 DEST 5583 bp EF1a Multisite Gateway cloning GOI puc ori Expression construct EF1a R4 att B Step II Retargeting (+) R4 integrase R4 attb R4 att R Ampicilin UMS Amp puc ori GOI EF1a Zeo TK-Hyg puc ori Amp R4 att L 11. Jump-In TI HEK 293 CHO-S I1 phic31 2 Hyg3456a Hyg qpcrb II7R4 8 Zeo/G418/Bsd 9 18
19 Lentiviral ipsc Reprogramming Particles ipsc hoct4 hsox2 hklf4 hc-myc hlin28 hnanog CMV-GFP hoct4-gfpipsc Go to Neon Transfection System DNA sirna 80% Neon Transfection 12 Neon Transfection 3 24 Go to pebna DEST Vector Kit pebna-destmultisite Gateway Protocol Exchange protocolexchange.community.invitrogen.com Epstein-BarroriP EBNA-1 B Go to BacMam Technology BacMam Go to Neon Transfection % % ES H Neon Transfection 19
20 BacMam EBNA ASC StemPro TARGET hesc BG01V Kit Jump-In TI Gateway BG01V Pseudo attp 3 PhiC31 pjti/zeo Hyg ptk Zeo R4-attP R4 pjti/eg ptk pef1a pef11 SMA AFP Hyg Zeo GFP R4-attL R4-attR 13. BG01VR4 phic31 StemPro TARGET BG01V Zeocin R4 attp R4 attb EF1a MultiSite Gateway DESTR4 TARGET R4 attb attp Zeocin EF1a Zeocin MultiSite Gateway DEST StemPro BG01V/EG EmGFP 14. EF1a-EmGFP StemPro TARGET BG01V 22 GFP3 GFP 20
21 Alkaline Phosphatase Live Stain (AP Live Stain) ESiPS Go to lifetechnologies.com/aplivestain Cell Line Contamination TaqMan PCR Go to Genomic Instability DNA STR SNP DNA Gene Expression TaqMan mrna 4 ml1 PCR 1 TaqMan PCR1 TaqMan Human Mouse Stem Cell Pluripotency Assay TaqMan Array 384 TaqMan ArrayApplied Biosystems 7900HT ViiA 7 PCR Go to Go to AP Live Stain MEFH9 21
22 Protein Expression FACS Go to Western-SuperStar Immunodetection System Western-SuperStar Immunodetection Western-SuperStar sirna TaqMan Protein Assays TaqMan Protein Expression Assay TaqMan 5TaqMan Gene Expression MicroRNA (mirna) Assay mrna mirna16 TaqMan Protein Assays Open Kit TaqMan Protein Assay Go to Cell Lysis Protein Expression Sample Preparation Kit Binding TaqMan Protein Assay Kit 3 oligo 5 oligo connector epitope 1 Target epitope 2 Target 60 min 10 min 15 min TaqMan Fast Real-Time PCR Applied Bios ystems Fast Real-Time PCR System Data Analysis TaqMan Protein Assay Data Analysis Software v1.0 (beta1) Fluorescence High concentration Intermediate concentration Low concentration Background Fold change Cycle number Day Final 45 min 16. TaqMan Protein Assay 22
23 Qtracker Labels Qtracker Cell Labeling Kit Qdot Qtracker Go to Alexa Fluor Dyes Alexa Fluor 30,000 Alexa Fluor 18 Go to PrestoBlue Cell Viability Reagent 10 PrestoBlue 10PrestoBlue Z Go to A B Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor 17. Geltrex 96H9 AB PrestoBlue 23
24 Click-iT EdU Cell Proliferation Assay Kits Click-iT EdU DNAClick-iT EdU Click-iT EdUEdU 19 BrdU DNA Go to Premo Autophagy Sensors ready-to-use CellLight Premo Autophagy Sensor 20 Premo FUCCI Cell Cycle Indicator Premo FUCCI Cell Cycle Sensor FUCCI BacMam CellLight Reagents CellLight 21 CellLight BacMam BacMamBacMam 19. DNA Click-iT EdU Alexa Fluor 647 Imaging Kit Alexa Fluor 488Hoechst Cascade Biologics HASMC Premo Autophagy Sensor LC3B-GFP CellLight Golgi-RFP Hoechst CellLight CellLight Histone 2B-RFP CellLight MAP4-GFP 24
25 RNAi Gene Regulation with TaqMan mirna Assays microrna mirnamirna RNA mrna mrnamrnamirna Go to Gene Knockdown with sirna sirna Go to Epigenetic Monitoring methylseqr Bisulfite Conversion KitPCR Go to Applied Biosystems Attune Acoustic Focusing Cytometer Attune Acoustic Focusing Cytometer Attune Acoustic Focusing Cytometer1 22Attune Cytometer Go to Attune Acoustic Focusing Cytometer 25
26 Ordering Information ESC ipsc CELLstart CTS A ml Collagenase Type IV g Dispase g DMEM ml DMEM/F ml ESC Qualified FBS ml FGF-Basic (recombinant human) µg Geltrex hesc-qualified A ml Geltrex RGF Matrix ml KnockOut DMEM ml KnockOut DMEM/F ml KnockOut SR ml KnockOut SR GF Cocktail A ml KnockOut SR XenoFree A ml LIF (recombinant human) PHC µg StemPro Accutase A ml StemPro hesc SFM A kit StemPro EZPassage Tool TrypLE Express with Phenol Red ml Trypsin-EDTA ml B-27 Serum-Free Supplement (50X) ml B-27 Supplement Minus AO (50X) ml B-27 Supplement Minus Insulin (50X), liquid (Custom) SA 10 ml B-27 Supplement Minus Vitamin A (50X) ml B-27 Supplement XenoFree (50X) A11576-SA 10 ml N-2 Supplement (100X), liquid ml G-5 Supplement (100X), liquid ml FGF-basic (recombinant human) µg Geltrex hesc-qualified A ml Geltrex RGF Matrix ml KnockOut DMEM ml KnockOut DMEM/F ml KnockOut SR ml KnockOut SR GF Cocktail A ml KnockOut SR XenoFree A ml MesenPRO RS Medium kit Neurobasal -A Medium ml Neurobasal -A Medium, without Phenol Red ml Neurobasal Medium ml Neurobasal Medium, without Phenol Red ml StemPro Adipogenesis Differentiation Kit A kit StemPro Chondrogenesis Differentiation Kit A kit StemPro Osteogenesis Differentiation Kit A kit StemPro hesc SFM A kit StemPro LipoMAX A ml StemPro MSC-SFM A kit StemPro MSC-SFM XenoFree A kit StemPro NSC-SFM A kit StemPro -34 SFM kit StemPro Human Adipose Derived Stem Cells R vial StemPro Human Adipose Derived Stem Cell Kit R kit Synth-a-Freeze Medium A ml Mouse (C57BL 6) Mesenchymal Stem Cells S ml Rat (SD) Mesenchymal Stem Cells S ml Rat Fetal Neural Stem Cells N ml Rat Fetal Neural Stem Cell Kit N kit Human Neural Stem Cells (H9-Derived) N ml Human Neural Stem Cell Kit (H9-Derived) N kit StemPro Rat Alk Phos Expressing Mesenchymal Stem Cells R ml StemPro Human Adipose Derived Stem Cells R vial StemPro Human Adipose Derived Stem Cell Kit R kit AlgiMatrix 3D Culture System 6-well (+ 50mL Firming Buffer) A plate AlgiMatrix 3D Culture System 6-well (+ 50mL Firming Buffer) A plates AlgiMatrix 3D Culture System 24-well plate AlgiMatrix 3D Culture System 24-well plates AlgiMatrix 3D Culture System 96-well plate plate AlgiMatrix 3D Culture System 96-well plate plates AlgiMatrix Firming Buffer A ml AlgiMatrix Dissolving Buffer A ml Attachment Factor(Gelatin) S ml CELLstart CTS A ml Collagen I, Bovine A ml Collagen I, Rat tail A ml Fibronectin Human, Plasma mg Fibronectin Natural Human PHE mg Fibronectin, Bovine Plasma mg Geltrex hesc-qualified A ml Geltrex LDEV Free RGF Matrix A ml Geltrex RGF Matrix ml Natural Mouse Laminin mg GIBCO recombinant growth factors Activin A (human) PHG µg Activin R1b (human) PHC µg Angiopoietin-2 (human) PHC µg B-DNF (human) PHC µg BMP-2 (human) PHC µg BMP-4 (human) PHC µg BMP-7 (active) (human) PHC µg BMP-7 (inactive) (human) PHC µg Brain-Derived Neurotrophic Factor (BDNF) (human) µg CNTF (human) PHC µg Cripto (human) PHC µg CTGF (human) PHG µg EGF (human) PHG µg EGF (Murine, Natural) µg EPO (human) PHC IU FGF-10 (human) PHG µg FGF-16 (human) PHG µg FGF-17 (human) PHG µg FGF-18 (human) PHG µg FGF-19 (human) PHG µg FGF-4 (human) PHG µg FGF-6 (human) PHG µg FGF-7 (KGF) (human) PHG µg FGF-8 (human) PHG µg FGF-8b (human) PHG µg FGF-9 (human) PHG µg FGF-basic (full length) (human) PHG µg FGF-basic (full length) (human) PHG µg FGF-basic (full length) (human) PHG µg FGF-basic (full length) (human) PHG mg FGF-basic (human) µg FGF-basic, AA (human) PHG µg FGF-basic, AA (human) PHG µg FGF-basic, AA (human) PHG µg FGF-basic, AA (human) PHG mg Flt-3L (human) PHC µg GDNF (human) PHC µg IL-10 (human) PHC µg IL-2 CTS (human) CTP µg IL-4 CTS (human) CTP µg IL-6 (human) PHC µg IL-7 CTS (human) CTP µg LIF (human) PHC µg 26
27 LIF (human) PHC µg Midkine (human) PHC µg Neurturin (human) PHC µg NT-4 (human) PHC µg Oncostatin M (human) PHC µg SCF c-kit Ligand (human) PHC µg SDF-1a (human) PHC µg Sonic Hedgehog C25II (mouse) PMC µg TGF- (human) PHG µg TGF- 1 (human) PHG µg TGF- 2 (human) PHG µg TNF- CTS (human) CTP µg Wnt-1 (human) PHC µg StemPro Accutase A ml StemPro EZPassage Tool TrypLE Express with Phenol Red ml TrypLE Select CTS A ml Trypsin-EDTA ml Dynabeads SSEA-4* * D 1 kit DynaMag CTS Human Dermal Fibroblasts, adult (HDFa) C-013-5C 1 vial Human Dermal Fibroblasts, neonatal (HDFn) C-004-5C 1 vial Human Epidermal Keratinocytes, adult (HEKa) C-005-5C 1 vial Human Epidermal Keratinocytes, neonatal (HEKn) C-001-5C 1 vial Medium 106 M ml EpiLife Medium M-EPI- 500-CA 500 ml EpiLife Defined Growth Supplement (EDGS) S ml, 100 X Human Keratinocyte Growth Supplement (HKGS) S ml, 100 X Low Serum Growth Supplement (LSGS) S ml, 50 X Supplement S7 S ml Keratinocyte AOF Growth Kit A kit Neon Transfection System Neon Transfection System Starter Pack MPK5000S 1 Neon Transfection System MPK Neon Transfection System Kits Neon Transfection System Kit (100 µl) MPK kit Neon Transfection System Kit (10 µl) MPK kit Neon Transfection System accessories Neon Transfection System Pipette MPP100 1 Neon Transfection System Pipette Station MPS100 1 Neon Transfection Tubes 100 MPT100 1 Secondary antibody conjugates (selected) Host Label donkey anti-goat IgG (H+L) A11055 Alexa Fluor 488 goat anti-mouse IgG (H+L) A11001 Alexa Fluor 488 goat anti-mouse IgM (µ chain) A21042 Alexa Fluor 488 goat anti-rabbit IgG (H+L) A11008 Alexa Fluor 488 goat anti-rat IgG (H+L) A11006 Alexa Fluor 488 rabbit anti-mouse IgG (H+L) A11059 Alexa Fluor 488 Qtracker Cell Labeling Kits Qtracker 525 Cell Labeling Kit Q25041MP 1 kit Qtracker 565 Cell Labeling Kit Q25031MP 1 kit Qtracker 585 Cell Labeling Kit Q25011MP 1 kit Qtracker 605 Cell Labeling Kit Q25001MP 1 kit Qtracker 655 Cell Labeling Kit Q25021MP 1 kit Qtracker 705 Cell Labeling Kit Q25061MP 1 kit Qtracker 800 Cell Labeling Kit Q25071MP 1 kit PrestoBlue Cell Viability Reagent A ml PrestoBlue Cell Viability Reagent A ml Jump-In TI Platform A kit Jump-In TI Gateway A kit Jump-In TI Gateway A pebna DEST Gateway A kit ipsc Lentiviral ipsc Reprogramming Particles HOCT4 A ul Lentiviral ipsc Reprogramming Particles HSOX2 A ul Lentiviral ipsc Reprogramming Particles HKLF4 A ul Lentiviral ipsc Reprogramming Particles HC-MYC A ul Lentiviral ipsc Reprogramming Particles HNANOG A ul Lentiviral ipsc Reprogramming Particles HLIN28 A ul Lentiviral ipsc Reprogramming Particles CMV-GFP REPORTER A ul Lentiviral ipsc Reprogramming Particles OCT4-GFP REPORTER A ul 27
28 2012 Life Technologies Japan Ltd.All rights reserved.the trademarks mentioned herein are the property of Life Technologies Corporation or their respecitve owners. B-27 is a registered trademark of Southern Illinois University. Taqman is a registered trademark of Roche Molecular Systems,Inc. For research use only. Not intended for any animal or human therapeutic or diagnostic use GIB024-B1203OB
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