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9 2) Pearse, A. G. E.. Histochemistry, Churchill, London ) Joyet-Lavergne, P.: Bull. hist. appl., 5; 331, ) Chevremont, M. & M. Fr4ddricq: Arch. Biol., 54; 589, ) Bennett, H. S.: Anat. Rec., 110; , ) Barrnett, R. J. & Seligman, A. M.. J. Nat. Cancer. Inst., 13; 215, ) Barrnett, R. J.: Nat. Cancer. Inst., 13; 905, ) Barrnett, R. J. & Seligman, A. M.: J. Nat. Cancer. Inst., 14; , ) Pearse, A. G. E.. J. Hiatochem. Cytochem., 1; , ) Gomori, G.: Quart, J. Micro. Science, 97; 1-9, ) Serra, J. A.. Stain Technol., 21; 5, ) Liaon, L.; C. r. Soc. biol., 106, 41, 1931; Arch Biol., 41; 343, ) Henle, J.,: Z. ration. Med., 24, 143, ) Romien, M.; Bull. histol. appl., 2: 185, ) Serra, J. A. & A. Queiroz-Lopes: Portug. acts biol., 1: 51, ) Sakaguchi, S.: J. Biochem. Jap., 5: 25, 1925; 37, ) Baker, J. R.: Ibid., 97, 161, ) Glenner, G. G.: J. Histochem., 5, 297, ) Serra. J. A.,: Portug. acta biol., 1, 1, 1944; Naturw., 32, 46, ) Serra, J. A. & Queiroz-Lopes A.: Port. acta biol., 1: 111, 1945.
10 C. A.: J. Histochem. Cytochem., 1; 39, ) Burstone, M. S.. Ibid., 3; 32, ) Weiss, L. P., K. C. Tsou & A. M. Seligman: J. Hist. Cytochem., 2; 29-49, ) Voss, H.: Z. mikr. Anat. Forsch., 49, 51, ) Wartenberg, H.; Acts, Histochem., 3, 145, ) Lison, L.: Histoehemie et Cytochimie Ani males, 2, ed., Ganthier-Villars, Paris, ) Pearse A. G. E.. Histochemistry, Churchill, London, 801, ) Greenstein, J. P.: J. Natl. Cancer Inst., 3, 61, ) Sugiura, T. & Ono, T.. Gann, 48, 77, ) Patt, H. M., Tyree, E. B., Strauble, R. L. & Smith, D. E.: Science, 110, 213, ) Michaelis, L.: Biochem. Ztschr., 234: 139, ) Rapkine, L.. Ann. Physiol. Physicochim. biol., 7, 382, ) Bracket, J.: Arch. Biol., 51, 167, ) Brachet, J.: Chem. Embryology. Intersci., New York, ) Kawamura, N. & Dann, K.: J. Biophys. Biochem. Cytol., 4, 5, , ) Annau, E. & Gossy, B.. Z. f. Krebsforsch., 40, 572, ) Klein, G. & Ziesse, W.: Z. f. Krebsforsch., 37, 323, ) Roberts, E. & Frankel, S.. Cancer Res., 9, 645, ) Dunn, M. S., Feaver E. R., E. A. Murphy: Cancer Res., 9, 306, ) Sauerlich, H. E. & Bauman, C. A.: Cancer, Res., 11, 67, ) Smith, L. C. & Rossi, F. M.: Proc. Soc. Expe. Biol. Med., 87, 643, ) Kit, S. & J. Awapara: Cancer Res., 13, ) Potter, B.: J. Invest. Dermat., 1, 33, , 1959.
11 A Histochemical Study on the Effect of the Antitumor Agent in Animal Tumor. Part I The Protein and Protein-Bound Amino-Base in Ascites Tumor Cell. By Kiyoshi Ishihara First Department of Surgery, Okayama University Medical School, Okayama, Japan. The protein and protein-bound amino-bases in the Ehrlich and Yoshida tumor cells were cytochemically examined. And the influences of the antitumor agents, i. e. Colchicine, Ni tromin, Sarcomycin and Mitomycin C on these tumor cells were also observed, and the following results were obtained. 1. The protein-bound SH showed the moderate reaction in cytoplasma and nucleus in both tumors. The nucleolus of the Ehrlich tumor cell showed a weak reaction, while that of the Yoshida tumor cell a remarkably positive reaction. By Colchicine administration, the stainability of SH was increased. 2. The protein-bound SH-SS reactions revealed almost the same localisation in both tu mors as the SH reaction, but the intensity of the reaction was stronger than that of SH. And the cytochemical pattern of SH-SS caused by the antitumor agents was almost the same as SH. 3. The protein-bound SS reaction was weak in both tumor cells. Generally, SS reaction diminished by the administration of the antitumor agents, especially by MitomycinC admi nistration to the Yoshida tumor, Nitromin and large amount of Colchicine to the Ehrlich tumor cells. Only by MitomycinC administration to the Ehrlich ascites tumor, the reaction of SH was increased. 4. In the Yoshida tumor, by the administration of all antitumor agents, the stainability of SH, SH-SS and SS were diminished, while in the Ehrlich tumor the changes of the staina bility were variable. 5. Tyrosine reaction was moderate in both tumor cells. The nucleus of the Ehrlich tumor showed the negative, and that of Yoshida tumor the slightly positive reaction. 6. The NH2 reaction was moderate in the cytoplasma of both tumors, and very strong in the nucleus. In the Yoshida tumor, the stainability was diminished by the administration of all antitumor agents, while in the Ehrlich tumor the reaction was diminished by the large amount of Colchicine, and the reaction was elevated by Nitromin and small dosis of Colchic ine. 7. The phenol and its compound reaction were moderate in the Ehrlich tumor and weak in the Yoshida tumor. By the administration of the antitumor agent, these reactions were generally diminished in both tumor cells, but Mitomycin C administration to the Ehrlich tumor these reactions were elevated.
12 Explanation of Figures I) Fig. 1-13: Ehrlich ascites tumor, ~1000. Fig. 1: SH-SS reaction, control sections; Fig. 2: NH2 reaction, control sections; Fig. 3: SH-SS reaction, Colchicine 50ƒÁ, after 6 hours; Fig. 4: NH2 reaction, Colchicine 50ƒÁ, after 6 hours; Fig. 5: SH-SS reaction, Colchicine 4ƒÁ, after 3 days; Fig. 6: SH reaction, Nitromin 0.5mg ~5; Fig. 7: SH-SS reaction, Nitromin 0.5mg ~5; Fig. 8: SS reaction, Nitromin 0.5mg ~5; Fig. 9: tyrosine reaction, Nitromin 0.5mg ~5; Fig. 10: SH reaction, Sarcomycin 5mg; Fig. 11: SS reaction, Sarcomycin 5mg; Fig. 12: SS reaction, Mitomycin C 40ƒÁ; Fig. 13: NH2 reaction, Mitomycin C 40ƒÁ. 2) Fig : Yoshida ascites tumor, ~ Fig. 14: SH-SS reaction, control sections; Fig. 15: tyrosine reaction, control sections; Fig. 16: NH2 reaction, control sections; Fig. 17: NH2 reaction, Colchicine 20ƒÁ; Fig. 18: SH-SS reaction, Nitromin 2mg; Fig. 19: phenol reaction, Nitromin 2mg; Fig. 20: phenol reaction, Sarcomycin 20mg; Fig. 21: SH reaction, Mitomycin C 0.2mg, after 9 hours; Fig. 22: tyrosine reaction, Mitomycin C 0.2mg, after 9 hours; Fig. 23: NH reaction, Mitomycin C 0.2mg, after 9 hours; Fig. 24: phenol reaction, Mitomycin C 0.2mg, after 24 hours.
13 動 物 腫 瘍 の 蛋 白基,各 種酵 素 お よび これ に及 ぼ す制 癌 剤 の影 響 に関 す る組 織 化 学 的 研究 石 原 論 文 附 図 37
14 38 石 石 原 原 論 文 清 附 図
15 動物 腫 瘍 の 蛋 白 基,名 種 酵 素 お よび これ に 及ぼ す 制 癌 剤 の影 響 に 関 す る組 織 化学 的研 究 石 原 論 文 附 図 39
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