Yeast Two-Hybrid System Kit N-G Wako & TranSort
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1 Yeast Two-Hybrid System Kit N-G Wako & TranSort
2 l (small unilamellar vesicle : SUV) DNA l l l l l l l 12ml35mm (4080 ) 37CO2 (5) 3 DNA0.252g50l DNA 440l2.520l 3.DNA 15DNA- l l l l l l 522mlPBS1.9ml 6DNA-24 37CO2 (5) 72mlPBS (35mm) Total lipids2mol Obika, S., Yu, W., Shimoyama, A., Uneda, T., Miyashita, K., Doi, T. and Imanishi, T. : Bioorg. & Med. Chem. Lett., 7, 1817 (1997) l (TMAG) DNA DNADNA DNA (35mm40) 1) Nobuyuki, H. and Kunio, Y. : J. Clin. Biochem. Nutr., 7, 175 (1989) 2) Mebio,26 (1992) 3) ) 12No.15 () 57 (1994) 5) 12 (No.13), 77 (1994) 6) 14 (No.4), 85 (1996) 7) Endoh, D. et al. : Jpn. J. Vet. Res., 43, 109 (1995)
3 (DOGS) DNA DNA 50 1) Jean-Paul Behr, et al. : Proc. Natl. Acad. Sci. USA., 86, 6982 (1989) 2) Corman, C. M. et al. : Mol. Cell. Biol., 2, 1044 (1982) 3) Chen, C. and Okayama, H. : Mol. Cell. Biol., 7, 2745 (1987) 4) Tsukamoto, M. et al. : Nature Genetics, 9, 243 (1995) l in vivoin vitro in vivo ) Otmane, B., Frank, L. et al. : Proc. Natl. Acad. Sci. USA., 92, 7297 (1995) 2) Regi, L., Yves, M. et al. : Molecular and Cellular Neuroscience, 7, 239 (1996) 3) Noguiezhellin, P. et al. : Proc. Natl. Acad. Sci. USA, 93, 4175 (1996) 4) Boussif, O. et al. : Gene Therapy, 3, 1076 (1996) DNA Calf thymus 1) 12, 184 (1994) 2) BIOTHRAPY, 8, 1265 (1994) 3) Kaneda, Y., Iwai, K. and Uchida, T. : J. Biol. Chem., 264, (1989) 4) Kaneda, Y., Iwai, K. and Uchida, T. : Science, 243, 375 (1989) 5) Kaneda, Y., Morishita, R. and Tomita, N. : J. Molec. Medicine, 73, 289 (1995) 1mgml 6) Morishita, R. et al. : Proc. Natl. Acad. Sci. USA, 92, 5855 (1995) 7) Yoshida, M. et al. : Proc. Natl. Acad. Sci. USA, 92, 9570 (1995) 8) Tomita, N. et al. : Gene Therapy, 3, 477 (1996) 9) Isaka, Y. et al. : Nature Medicine, 2, 418 (1996) 10) (), 104 (1996)
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6 DupLEX-A TM FieldSong 1) Gal4p two-hybrid LexA Two-Hybrid- (Bait) (Target) DupLEX-A TM DNA LexA Acid BlobB42LexA LexA B42 B42 false positive B42 Bait B42 HA tagbaitpotential positive Bait Bait BaitLex A l 1) Fields, S. and Song, O. : Nature, 340, 245 (1989) 2) Chien, C. T. et al. : Proc. Natl. Acad. Sci. USA, 88, 9578 (1991) 3) Gyuns, J. et al. : Cell, 75, 791 (1993) (DKT-100) DupLEX-A Yeast Two-Hybrid System Kit 1kit 88,000
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9 1 for 7days in 16hr light and 8hr darkness per day 2 for 7days in complete darkness 1) Maruyama, K. and Sugano, S. : Gene, 138, 171 (1994) 2) Tokunaga, K., Taniguchi, H., Yoda, K., Shimizu, M. and Sakiyama, S. : Nucleic Acids Res., 14 (6), 2829 (1986) 3) Leader, D. P., Gall, I., Campbell, P. and Frischauf, A. -M. : DNA, 5, 235 (1986) 4) Peter, B., Man, Y. M., Begg, C. E., Gall, I. and Leader, D. P. : J. Mol. Biol., 203 (3), 665 (1988) 5) in preparation l A. niger (AATase) Alcohol Acetyltransferase ph AATase CoA E. C. : Aspergillus niger 1.5 Umg llllllllllllllllllllllllllllllllllllllllllllllllll 200munit 30,000 1Toshitaka, M. et al. : Bioscience Biotechnology Biochemistry, 57, 2094 (1993) 2) Yoshioka, K. et al. : Agric. Biol. Chem., 46, 2155 (1982)
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