Symposium mmHg Tyrode Na channel optical mapping system APD dispersion connexin Sprague Dawley rats male 8 weeks old

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あけなおかみこ
7 years ago
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1 26 Symposium 41 Connexin43 40mmHg SD rat n Tyrode Na channel optical mapping system OMP action potential duration dispersion APD dispersion connexin43 Cx OMP burst pacing 5 APD dispersion connexin43 5 APD dispersion connexin43 1, Bonpei Takase, et al. : Significant role of action potential duration dispersion and connexin 43 in lethal arrhythmogenesis in hemorrhagic shock heart: optical mapping analysis 1060

2 Symposium mmHg Tyrode Na channel optical mapping system APD dispersion connexin Sprague Dawley rats male 8 weeks old g n 32 ketamine hydro chloride 5mg/kg abdominal aorta catheter abdominal aorta catheter Total 30 blood loss n 8 1 optical mapping analysis 37 Tyrode CaCl 2 [2], NaCl [140], KCl [4.5], dextrose [10], MgCl 2 [1], and HEPES [10, ph 7.4], in mmol/l Tyrode Na di 4 ANEPPS 15 mol/ L 40mL butanedione monoxime Wako Chemical, Tokyo, Japan, 20 mm optical mapping analysis high quality charge couple 1061

3 28 Symposium 41 Recording images by CCD camera Cardiac imaging(right ventricle) Cardiac imaging(left ventricle) di 4 ANEPPS BDM Normal sinus rhythm Conduction velocity pattern(pacing) 2 optical mapping system device CCD camera Leica , Geneva, Switzerland 4 ms APD commercialized software Ultima 6006 Sei Media, Inc., Tokyo, Japan 2 5 5mm APD APD APD APD 60ms APD APD APD dispersion ms APD dispersion burst pacing, 5, 50, 100 V 40ms interval, 20 trains voltage 3 4A 2 connexin43 4 paraformaldehyde phosphate buffer solution Wako Pure Chemical Industries Ltd ethanol paraffin connexin43 anti connexin43 monoclonal antibody 1 2,000, Sigma Aldrich, St. Louis, USA gap junction connexin43 3 APD dispersion connexin43 2 ANOVA Bonferroni post hoc 1062

4 Symposium Action potential duration dispersion APDd Maximum APD minimum APD Normal control Mild Moderate Severe Impairment 3 optical mapping system action potential duration dispersion 2 p 0.05 A 2 1 optical mapping analysis APD dispersion 4B 3 3 optical mapping system ms ms 39 3ms B C D E 4 burst pacing 1063

5 30 Symposium 41 Normal control Shock+5% アルブミン Shock+ 生理食塩水 Shock+ 洗浄赤血球 5 Normal control Shock+5% アルブミン Shock+ 生理食塩水 Shock+ 洗浄赤血球 6 action potential duration dispersion ms APD dispersion 3 normal control normal control vs 14 2ms vs 13 3ms, NS 5 3 moderate severe impairment pattern APD dispersion 34 27ms 38 9ms p 0.05 APD

6 Symposium Normal control Shock alone Shock+5% アルブミン / 生理食塩水洗浄赤血球 100μm 100μm 100μm 7 connexin43 6 burst pacing 4A 5 4C D 4E 2 connexin43 connexin43 7 connexin43 connexin43 5 connexin43 3 APD gap junction connexin optical mapping system burst pacing 5 optical mapping system APD dispersion gap junction connexin

7 32 Symposium 41 4, 7, optical mapping system gap junction connexin43 1 Rubin LJ. Introduction: Diagnosis and management of pulmonary arterial hypertension: ACCP evidence based clinical practice guideline. Chest 2004;126:7S 10S. 2 Kuriyama T. Clinical aspect of precapillary pulmonary hypertension, Nihon Kyobu Sikkan Gakkai Zasshi Jpn Respir Disease 1992;30: Umar S, Lee JH, da Lange E, Iorga A, Partow Navid R, Bapat A, et al. Spontaneous ventricular fibrillation in right ventricular failure secondary to chronic pulmonary hypertension. Circ Arrhythm Electrophysiol 2012;5: Koura T, Hara M, Takeuchi S, Ota K, Okada Y, Miyoshi S, et al. Anisotropic conduction properties in canine atria analyzed by high resolution optical mapping: preferential direction of conduction block changes from longitudinal to transverse with increasing age. Circulation 2002;105: Miyazaki K, Hori S, Inoue S, Adachi T, Bessho M, Kuwahira I, et al. Characterization of energy metabolism and blood flow distribution in myocardial ischemia in hemorrhagic shock. Am J Physiol 1997;273:H Sasano C, Honjo H, Takagishi Y, Uzzaman M, Emdad L, Shimizu A, et al. Internalization and dephosphorylation of connexin43 in hypertrophied right ventricles of rats with pulmonary hypertension. Circ J 2007;71: Nagami Y, Kinoshita M, Takase B, Ogata Y, Saitoh D, Kikuchi M, et al. Liposome encapsulated hemoglobin transfusion rescues rats undergoing progressive hemodilution from lethal organ hypoxia without scavenging nitric oxide. Ann Surg 2008;248: Shono S, Kinoshito M, Takase B, Nogami Y, Kaneda S, Ishihara M, et al. Intraosseous transfusionj with liposome encapsulated hemoglobin improves mouse survival after hypohemoglobinemic shock without scavenging nitric oxide. Shock 2011; 35: Adachi T, Hori S, Miyazaki K, Nakagawa M, Inoue S, Ohnishi Y, et al. Inhibition of nitric oxide synthesis aggravates myocardial ischemia in hemorrhagic shock in constant pressure model. Shock 1998;9: Nakagawa M, Hori S, Adachi T, Miyazaki K, Inoue S, Suzuki M, et al. Adenosine triphosphate sensitive potassium channels prevent extension of myocardial ischemia to subepicardium during hemorrhagic shock. Shock 2008;30:

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