Vol.7November 216 3 DNA QIAamp DNA Mini Kit QIAcube QIAamp DNA Mini Kit 2 M Lung Kidney Liver Skin and muscle Whole blood Buffy coat Lymphocytes M QIAcube DNA 48.5 kb QIAamp DNA Mini Kit (5) 5134 25, QIAamp DNA Mini Kit (25) 5136 111, QIAamp DNA Mini QIAcube Kit (24) 51326 129, 5 kb DNA DNA 3 µg 5 kb DNA DNA DNeasy Plant Mini Kit QIAcube DNA Absorbance 1. 3 3 µg DNA QIAcube DNeasy Dellaporta DNA CTAB DNeasy Plant Mini Kit (5) 6914 26,5 DNeasy Plant Mini Kit (25) 6916 119, DNeasy Plant Maxi Kit (24) 68163 41,5 CTAB. 22 26 28 32 Wavelength (nm) DNA Dellaporta CTAB DNA DNA 26 nm Sample to Insight
QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzol QIAzol Lysis Reagent (2 ml) 7936 38,5 MaXtract High Density (2 x 1.5 ml) 1 5 µl/tube 12946 28,5 MaXtract High Density (2 x 2 ml) 1 75 µl/tube 12956 27,5 MaXtract High Density (1 x 15 ml) 1 6 ml/tube 12965 23,5 MaXtract High Density (25 x 5 ml) 5 2 ml/tube 12973 2, MaXtract MaXtract High Density MaXtract MaXtract RNA RNA RNAlater RNA Stabilization Reagent A C 3 2 4 4 7 RT 24 37 B C 16 18 2 Number of freezethaw cycles RNAlater RNA Stabilization Reagent RNA RNAlater 2 8 GAPDH RNA A RNA RNA RNA B 2 25 RNA Cat. no. RNAlater RNA Stabilization Reagent (5 ml) 25 x 2 mg 7614 8,7 RNAlater RNA Stabilization Reagent (25 ml) 125 x 2 mg 7616 38,5 2 Product News Lite Vol. 7/216
AllPrep Kits Allprotect Tissue Reagent DNA/RNA/ AllPrep Kits DNA/RNA/ Allprotect DNA RNA RNA gdna gdna RNA AllPrep DNA/RNA AllPrep DNA/RNA Mini Kit (5) 824 6, AllPrep DNA/RNA Micro Kit (5) 8284 81,5 AllPrep DNA/RNA/Protein Mini Kit (5) 84 7, AllPrep DNA/RNA FFPE Kit (5) 8234 74, Allprotect Tissue Reagent 7 12 Fold change in transcript level 1 1.1.1 in vivo.1 2 h 4 h Allprotect 24 h 2 h 4 h PBS 24 h Allprotector DNA Allprotect Reagent RNA 25 Allprotect Reagent PBS Madh7 RT-PCR Allprotect Reagent RNA RNA Allprotect Allprotector 1 DNA RNA Allprotector DNA RNA Allprotector DNA RNA Allprotect Tissue Reagent (1 ml) 7645 72,5 Product News Lite Vol. 7/216 3
QIAGEN Plasmid Plus Kits Attractene Transfection Reagent Effectene Transfection Reagent DNA QIAGEN Plasmid Plus Kits QIAGEN Plasmid Plus Kit 2 * * * Midi/Maxi 2 Mega/Giga 4 5 pbluescript puc pgem pbr322 EU/µg DNA 1.4 1.2 1..8.6.4.2 CompactPrep QIAGEN Plasmid Plasmid Plus S P M DNA QIAGEN Plasmid Plus Midi Kit 2 35 ml 1 25 µg 5 ml 3 1 µg 25/1 12943/12945 35,/127, QIAGEN Plasmid Plus Maxi Kit 8 13 ml <1 mg <2 ml 5 25 µg 25/1 12963/12965 75,/284, QIAGEN Plasmid Plus Mega Kit 5 ml <2.5 mg 5 ml <2.5 mg 5 12981 32, QIAGEN Plasmid Plus Giga Kit 2.5 L <1 mg 2.5 L <1 mg 5 12991 68, QIAvac 24 Plus 1 24 Spin Column 19413 44,5 QIAvac Holder Mega Giga 19418 18, QIAvac Connecting System 24 VacValves 19419 137, QIAvac DNA Attractene Transfection Reagent Effectene Transfection Reagent Relative transfection efficiency (%) 12 1 8 6 4 2 Attractene Reagent L.4 µg DNA.5 µg DNA with enhancer.5 µg DNA without enhancer Transfection efficiency (1 4 x RLU) 5 4 3 2 1 Effectene Reagent Reagent L Reagent F Transfection reagent I. Clavereau, D. Petitprez, and I. Van Seuningen, Unité INSERM 377, Place de Verdun, Lille Cedex, France pgfp green fluorescent protein HepG2 FACS Attractene Effectene F9 48 Effectene www.qiagen.com/jp/transfection/celllistlist/ Attractene Transfection Reagent (1 ml) 315 55, Attractene Transfection Reagent (4 x 1 ml) 317 182, Effectene Transfection Reagent (4 x 1 ml) 31427 223, Effectene Transfection Reagent (1 ml) 31425 69,5 Effectene Transfection Reagent (1 ml) 112829 3,399, 4 Product News Lite Vol. 7/216
TopTaq Master Mix Kit QIAGEN Multiplex PCR Plus Kit QIAGEN OneStep Ahead RT-PCR Kit TopTaq Master Mix Kit 4 3' 5' TopTaq Stabilizer 5' 3' PCR TopTaq Master Mix Kit (25) 243 27, TopTaq DNA Polymerase TopTaq Stabilizer TopTaq Stabilizer Primer Template DNA PCR QIAGEN Multiplex PCR Plus Kit M 1.25 U 2.5 U 3.75 U 5 U Standard Supplier A II QIAGEN M x Hot Start Taq Factor MP PCR GC 2 Q-solution QIAGEN Multiplex PCR Plus Kit (3) 26151 11,5 QIAGEN Multiplex PCR Plus Kit (1) 26152 33,5 Fast PCR RT-PCR QIAGEN OneStep Ahead RT-PCR Kit 1 QIAGEN 1 1 1 GAPDH Competitor A 1 1 1 Competitor B 1 1 1 QIAGEN OneStep Ahead RT-PCR Kit (5) 22211 23,5 Hela RNA1 1 1 pg GAPDH 1 RT-PCR QIAGEN OneStep Ahead RT-PCR Kit 1 pg RNA QIAGEN OneStep Ahead RT-PCR Kit (2) 22213 71,5 QIAGEN OneStep Ahead RT-PCR Kit (2) 22216 629, Product News Lite Vol. 7/216 5
mirneasy Mini Kit Micro Kit PCR mirna lncrna mrna RNA mirneasy Mini Kit Micro Kit 1 QIAcube.8 mirneasy Precipitation mirneasy 18 nt RNA RNA mrna mirna lncrna mrna QIAGEN PCR mirneasy Mini Kit (5)* 2174 46, mirneasy Micro Kit (5)* 21784 47,5 OD.6.4.2 -.2 22 23 24 25 26 27 28 29 3 31 32 RNA Jurkat 1 x 1 6 T mirneasy Mini Kit RNA OD 26 nm 23 nm * mirneasy www.qiagen.com RT 2 Profiler PCR Array mirna miscript PCR System lncrna RT 2 lncrna PCR System 4 PCR PCR PCR 216 12 22 mrna RT 2 Profiler PCR Array mrna PCR lncrna RT 2 lncrna PCR Array lncrna PCR mirna miscript mirna PCR Array microrna PCR 96 2 PCR 2 + 96 6 PCR 6 + 384 96 x 4 1 PCR 1 + 144,5 86,7 261,5 156,9 111,5 66,9 www.qiagen.com/jppromo-pcrarraytrial 6 Product News Lite Vol. 7/216
mirneasy Serum/Plasma Kit mirna RNA mirneasy Serum/Plasma Kit (5) 2 µl 18nt mirna RNA 2 µl miscript mirna PCR Array mirneasy Serum/Plasma Spike-In Control mir-39 miscript PCR System mirna 14 µl QIAcube A Log1 p 5 4 3 2 1 B Log p 1.74 Down-regulation Up-regulation 9.49 8.24 6.99 5.74 4.49 3.24 1.99.74 RMRP PVT1 1 6 4 2 2 4 Log 2 NSCLC 2.18 1.18.18.82 1.18 2.82 3.82 4.82 5.82 Log 2 NSCLC Upregulated Unchanged Downregulated Downregulated Upregulated mirna lncrna mirneasy Serum/Plasma Kit non-small cell lung cancer NSCLC 2 µl RNA mirna lncrna NSCLC A Human Serum/Plasma miscript mirna PCR Array B RT 2 PreAMP Human RT 2 lncrna Cancer Pathway Finder PCR Array RNA Application Note www.qiagen.com/download-an-rna mirna www.qiagen.com/webinar-mirna qpcr mirna miscript mirna PCR Array www.qiagen.com/jpmiscriptpcrarray Cat. no. mirneasy Serum/Plasma Kit (5) QIAzol C. elegans mir-39 miscript Primer Assay 217184 62, mirneasy Serum/Plasma Spike-In Control C. elegans mir-39 mirna mimic 1 pmol/tube 21961 8,9 Product News Lite Vol. 7/216 7
QIAGEN 39 216 11 3 12 2 QIAGEN QIAGEN Check! www.qiagen.com/webinar-japan QIAprep QIAGEN QIAGEN QIAprep 2. Spin Column 1 12, www.qiagen.com/qiagen-club Tel 3-689-73 E-mail epromo-jp@qiagen.com Trademarks: QIAGEN, QIAamp, QIAcube, QIAzol, AllPrep, CompactPrep, DNeasy, Effectene, miscript, TopTaq (QIAGEN Group); FACS (Becton Dickinson and Company). RNAlater is a trademark of AMBION, Inc., Austin,Texas and is covered by various U.S. and foreign patents. Cat. no. 216 QIAGEN, all rights reserved. www.qiagen.com 14-54 3-13-1 Forefront Tower II Tel:3-689-73 Fax:3-5547-818 E-mail:techservice-jp@qiagen.com 232435 11/216