ORYZA POLYAMINE ORYZA POLYAMINE -P -PC -LC 1. Ver.1.0 MM
ver. 1.0MM 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504 (1983). 2) Ha.C. et al., The natural polyamine spermine functions directly as a free radical scavenger. Proc. Natl. Acad. Sci. USA 95, 11140-45 (1998). 3) Fujisawa S. et al., Kinetic evaluation of polyamines as radical scavengers. Anticancer Res. 25, 965-70 (2005). 4) Soda K. et al., Spermine, a natural polyamine, suppresses LFA-1 expression on human lymphocyte. J. Immunol. 175, 237-45 (2005). 5) Zhang M. et al., Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells:a counterregulatory mechanism that restrains the immune response. J. Exp. Med. 185, 1759-68 (1997). 6) Gugliucci A. et al., The polyamines spermine and spermidine protect proteins from structural and functional damage by AGE precursors: a new role for old molecules?life Sci. 72, 2603-16 (2003). 7) Gugliucci A., Alternative antiglycation mechanisms: are spermine and fructosamine-3-ki nase part of a carbonyl damage control pathway? Med. Hypotheses 64 2603-16 (2005). 8) Soda K., Polyamine intake, dietary pattern, and cardiovascular disease. Med. Hypotheses. 75,299-301 (2010). 9) De la Pena N.C. et al., Inhibition of platelet aggregation by putrescine, spermidine, and spermine in hypercholesterolemic rabbits. Arch. Med. Res.75, 299-301 (2000). 1
ver. 1.0MM 10)Ramot Y. et al., Polyamines and hair: a couple in search of perfection. Exp. Dermatol. 19, 784-90 (2010). 11) Ramot Y., Spermidine promotes human hair growth and is a novel modulator of human epithelial stem cell functions. Plos One, 6,1-11(2011). 2. 1 2 20 1 H N NH H 2 N 2 H 2 N N H H N NH2 H 2N NH 2 1. ODC 2 12,13) 3 14) 4 2
ver. 1.0MM ODC S- 2. 3. 4. 3
ver. 1.0MM 12) Yoshinaga K. et al., Age-related changes in polyamine biosynthesis after fasting and refeeding. Exp. Gerontol. 28, 565-72 (1993). 13) Scalabrino G. et al., Polyamines in mammalian ageing: an oncological problem, too? A review. Mech. Ageing Dev. 26, 149-64 (1984). 14) Soda K. et al., Long-term oral polyamine intake increases blood polyamine concentrations. J Nutr. Sci. Vitaminol. 55, 361-6 (2009). 5 5 4
ver. 1.0MM 3. 1 6 6. Atiya A. M. et al., Polyamines in foods: development of a food database. Food Nutr. Res. 55,3402 (2011) = 352 1000 250 (diamine oxidase) 5
ver. 1.0MM 6
ver. 1.0MM 4. (1) 7
ver. 1.0MM 8 40~60 % 8. 96 510 3 / ( 0.33 %)110100 µg/ml 100 µl 24 MTT Assay p0.01 p0.05 mean±sen=3) (µg/ml ) 8
ver. 1.0MM 9. 24 510 4 / ( 0.33 %)110100 µg/ml 500µL 1~2 Total RNA c DNA PCR (2) 9
ver. 1.0MM 10. (3) () 1 0.005% 10 2060 2 1 1 2 87 % 11 10
ver. 1.0MM 1 0.005 % 1,3- ph () :0.33 % (%) 2.0 8.0 5.0 4.0 6.0 2.0 0.1 0.1 4.0 6.0 0.1 61.18 1.52 0.005 % 11. 11
ver. 1.0MM (4) Normal ( 5 ) ( 6 ) ( 7 ) ( 8 ) ( 9 ) (10) (11) (12) 12
ver. 1.0MM 5. 1 80 ( 13) 1 100 1 100 6. ph 1 ( 14). 13
ver. 1.0MM 7. -P 50 ~ 100 mg / 8. -P 100 g 6.3 g 9.0 g (6.25) 1.0 g 34.4 g 40.8g 100g (+++) 225 kcal 8.5g 68.8mg * 4; 9; 4; 2 -P : 24 5 17 No. 120508128-001-01 9. (1) 535 23 5 17 14
ver. 1.0MM (2) (LD 50 ) 30 g 5 ICR, 2,000 mg/kg 23±2 50±10% 14 LD 50 2,000 mg/kg (3) Salmonella typhimuriumta100ta98 Ames 19.52,500 µg/plate 10. 11. -P () 5 kg -PC () 5 kg -LC ) 5kg 12. 15
ver. 1.0MM 13. P INCI PC ORYZA SATIVA (RICE) EXTRACT DEXTRIN CITRIC ACID LC BG WATER BUTYLENE GLYCOL ORYZA SATIVA (RICE) EXTRACT CITRIC ACID 16
ver. 1.0MM 0.2 % (HPLC ) 10.0 % (1 g1052 ) (1) 20 ppm () Pb (2) 1 ppm ( 3 B ) As 2 O 3 1 10 3 g () 1 10 2 g ( ) (BGLB ) 53 % 30 % 17 % 100 % 17
ver. 1.0MM 0.2 % (HPLC ) 10.0 % (1 g1052 ) (1) 20 ppm ( 2 ) Pb (2) 1 ppm ( 3 ) As 2 O 3 1 10 2 g () 1 10 2 g ( ) (BGLB ) 53 % 30 % 17 % 100 % 18
ver. 1.0MM 0. 004 % (HPLC ) (1) 10 ppm ( 2 ) Pb (2) 1 ppm ( 3 ) As 2 O 3 1 10 2 g () 1 10 2 g ( ) (BGLB ) 89.14 % 10 % 0.65 % 0.21 % 100 % 19
ver. 1.0MM OEM 493-8001 TEL(0586)86-5141() FAX(0586)86-6191 URL/http://www.oryza.co.jp/ E-mail: info@oryza.co.jp 101-0041 1-24-10 5F TEL(03)5209-9150 FAX(03)5209-9151 E-mail: tokyo@oryza.co.jp * * 2012 5 21