" " "
" n " n n " n
" n n n " n n n n
" n LGPSSKQTGKGW-SRIWDN! + +! LN-ITKSAGKGAIMRLGDA! " n -------TGKG--------!! -------AGKG--------!
" n w w w " n w w
" " "
11
12
" n w w w " n w w
A! M! O! A! I! M! S! S! AIM-S! A-MOS!
A! I! M! S! A! M! O! S!
1 2 3 4! A I M S! A M O S!
A T A T G C C T T! 0-1 -2-3 -4-5 -6-7 -8-9! A -1 1 0-1 -2-3 -4-5 -6-7! A -2 0 1 1 0-1 -2-3 -4-5! G -3-1 0 1 1 1 0-1 -2-3! C -4-2 -1 0 1 1 2 1 0-1! C -5-3 -2-1 0 1 2 3 2 1! T -6-4 -2-2 0 0 1 2 4 3! G -7-5 -4-2 -1 1 0 1 3 4! T -8-6 -4-3 -1 0 1 0 2 4!
ATATGCCT-T! A-A-GCCTGT! A T A T G C C T T! 0-1 -2-3 -4-5 -6-7 -8-9! A -1 1 0-1 -2-3 -4-5 -6-7! A -2 0 1 1 0-1 -2-3 -4-5! G -3-1 0 1 1 1 0-1 -2-3! C -4-2 -1 0 1 1 2 1 0-1! C -5-3 -2-1 0 1 2 3 2 1! T -6-4 -2-2 0 0 1 2 4 3! G -7-5 -4-2 -1 1 0 1 3 4! T -8-6 -4-3 -1 0 1 0 2 4!
ATGCCT-T! AAGCCTGT! A T A T G C C T T! 0 0 0 0 0 0 0 0 0 0! A 0 1 0 1 0 0 0 0 0 0! A 0 0 1 1 1 0 0 0 0 0! G 0 0 0 1 1 2 1 0 0 0! C 0 0 0 0 1 1 3 2 1 0! C 0 0 0 0 0 1 2 4 3 2! T 0 0 1 0 1 0 1 2 5 4! G 0 0 0 1 0 2 1 1 4 5! T 0 0 1 0 2 1 2 1 3 5!
Ala 1! Arg -5 7! Asn -5-1 7! Asp -5-2 2 8! Cys -5-4 -2-4 13! Gln -5 1 0 0-3 7! Glu -5 0 0 2-3 2 6! Gly -5-3 0-1 -3-2 -3 8! His -5 0 1-1 -3 1 0-2 10! Ile -5-4 -3-4 -2-3 -4-4 -4 5! Leu -5-3 -4-4 -2-2 -3-4 -3 2 5! Lys -5 3 0-1 -3 2 1-2 0-3 -3 6! Met -5-2 -2-4 -2 0-2 -3-1 2 3-2 7! Phe -5-3 -4-5 -2-4 -3-4 -1 0 1-4 0 8! Pro -5-3 -2-1 -4-1 -1-2 -2-3 -4-1 -3-4 10! Ser -5-1 1 0-1 0-1 0-1 -3-3 0-2 -3-1 5! Thr -5-1 0-1 -1-1 -1-2 -2-1 -1-1 -1-2 -1 2 5! Trp -5-3 -4-5 -5-1 -3-3 -3-3 -2-3 -1 1-4 -4-3 15! Tyr -5-1 -2-3 -3-1 -2-3 2-1 -1-2 0 4-3 -2-2 2 8! Val -5-3 -3-4 -1-3 -3-4 -4 4 1-3 1-1 -3-2 0-3 -1 5! A R N D C Q E G H I L K M F P S T W Y V!
" n n n n w w n n w
" n n n w w n
" n n " n n n n n
" Woese, C. R., Kandler, O. and Wheelis, M. L. (1990) Towards a natural system of organisms: Proposal for the domains, Archaea, Bacteria and Eukarya. Proc. Natl. Sci. USA, 87, 4576-4579. " Gibbs, A. J. and McIntyre, G. A. (1970) The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences. Eur. J. Biochem., 16, 1-11. " Needleman, S. B. and Wunsch, C. D. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol., 48, 443-453. " Smith, T. F. and Waterman, M. S. (1981) Identification of common molecular subsequences. J. Mol. Biol., 147, 195-197. " Gotoh, O. (1982) An improved algorithm for matching biological sequences. J. Mol. Biol., 162, 705-708. " Dayhoff, M. O., Schwartz, R. M. and Orcutt, B. C. (1978) A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure, Vol. 5, Suppl. 3, pp. 345-352. " Henikoff, S. and Henikoff, J. G. (1992) Amino acid substitution matrices from protein blocks. Proc. Natl. Acad. Sci. USA, 89, 10915-10919. 29
" n n " n n " n n " n n
31
" n n " n n n n " n n n
" n n n n " n
プロテオーム インタラクトーム解析と バイオインフォマティクス " プロテオーム n SWISS-2DPAGE ２次元電気泳動 質量分析 " インタラクトーム n n n n n 相互作用している 結合し ている タンパク質のペア を網羅的に調べる 酵母２ハイブリッド 質量分析 タンパク質チップ インタラクトームとも言う " 類似発現タンパク質のクラス " タリング タンパク質相互作用ネット ワーク構築 34
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" " n n " n n