Agilent 2100 1 DNA RNA
Lab-on-a-Chip Agilent 2100 1999 Agilent 2100 Lab-on-a-Chip Agilent 2100 RNA DNA SDS-PAGE Agilent 2100 Agilent 2100 Agilent 2100 : (1 4 µl) 30 21 CFR Part 11 Agilent 2100 Agilent 2100 1. 2. 3. Agilent 2100 ( ) 4. 1. 2. 3. 4. Agilent 2100 ( PC ) 2
1 Agilent 2100 / 2100 Lab-on-a-Chip DNA RNA 1 2100 Lab-on-a-Chip 2100 SDS-PAGE 2100 1. 5 µl 2. / 3. 30 RNA QC RIN Agilent 2100 5,000 qpcr RNA PCR/multiplex PCR RT-PCR DNA 1. 2. 3. 4. ( ) ( ) 1. 2. 1. 3. 4. 3
RNA RNA total RNA? mrna Cy5 RNA? RNA total RNA mrna RNA RNA Integrity Number (RIN) RNA 1 µl total RNA 50pg RNA RNA RIN total RNA mrna RNase Free RNase Free RNase Free EtBr) µl 10 RIN = 9.2 8 6 4 2 0 19 24 29 34 39 44 49 54 59 64 69 Intact total RNA [ ] 10 8 6 4 2 0 RIN = 5,8 Partially degraded RNA 19 24 29 34 39 44 49 54 59 64 69 [ ] RNA RNase Agilent 2100 () total RNA RNA Integrity Number (RIN) RNA 4
DNA DNA DNA DNA multiplex PCR PCR DNA (Laser Induced Fluorescence) 0.1 ng DNA / (2 ) 3 (25-1000bp 100-7500bp 100-12000bp ) PCR : PCR multiplex PCR RT-PCR PCR (EtBr) µl multiplex PCR PCR PCR RNA 1000 : 13 primer (99 955 bp 13 ) multiplex PCR QIAGEN ( ) 5
Protein Agilent 2100 SDS-PAGE Agilent 2100 ( ) 10 45 IQ OQ/PV 1 4 µl Protein 80 Protein 230 QA/QC 1 2 40 C 12 Agilent 2100 6
* * 2100 Agilent Lab-on-a-Chip Annexin V Caspase-3 / GFP 10 µl ( 20,000 2,500 ) 6 Agilent 2100 DNA RNA (GFP) Cy5 sirna Annexin V -3 DNA (DNA ) Annexin V (PS: ) PS Annexin V Cy5 Annexin V 7
Agilent 2100 RNA DNA Agilent 2100 total RNA RNA Integrity Number (RIN) (IQ OQ) 21 CFR Part 11 ( ) 1 48 (Web ) RNA DNA XML 48 ( 48 ) 8
Agilent 2100 Agilent 2100 IQ OQ/PV * ( ) (GLP) (GMP) Agilent 2100 DQ IQ OQ/PV 2100 21 CFR Part 11 2100 2100 (DQ) DQ 2100 2100 ISO/IEC Guide 22 CEN/ CENELEC EN 46014 (IQ) IQ Agilent 2100 2100 (OQ) OQ (PQ) PQ PQ 2100 *2001 "B" "C" 1 Dr. Ludwig Huber 9
Agilent 2100 1 Agilent 2100 Agilent 2100 (PC ) 1 Agilent 2100 1 PC 1 2 : LabChip Agilent 2100 * (IQ) (OQ) Agilent 2100 IQ OQ ( ) *2001 "B" "C" Agilent 2100 DNA RNA 10
: RNA DNA Agilent RNA 6000 Agilent RNA 6000 Agilent RNA 6000 Agilent RNA 6000 Agilent total RNA mrna Assay total RNA Assay mrna Assay Small RNA Assay 25 500 ng/µl 25 250 ng/µl 50 2000 pg/µl 5 500 ng/µl 25 250 ng/µl 50 5,000 pg/µl 250 5,000 pg/µl ( ) (S/N > 3) ( ) (S/N > 3) 20% CV 20% CV 30% CV 30% CV 25%CV () * 100 mm Tris 100 mm Tris 50 mm Tris 50 mm Tris 125 mm NaCl 125 mm NaCl 50 mm NaCl 50 mm NaCl 15 mm MgCl2 15 mm MgCl2 10% CV 10% CV 20% CV 20% CV 25% CV 30 30 30 30 30 12 / 12 / 11 / 11 / 11 / 1 µl 1 µl 1 µl 1 µl 1 µl 3 3 3 3 3 () * 6 150 nt 50 pg/µl(3<sn) 40 nt ) RNA 100 ng/µl Agilent DNA 1000 Assay Agilent DNA 7500 Assay Agilent DNA 12000 Assay 25 1,000 bp 100 7,500 bp 100 12,000 bp ± 5 bp 25 100 bp ± 5% 100 1,000 bp ± 5% 100 1,000 bp ± 5% 100 500 bp ± 15% 1,000 7,500 bp ± 15% 1,000 12,000 bp ± 10% 500 1,000 bp ** ± 10%* ± 10% ± 15% ** 5% CV 5% CV 5% CV ** 20% CV 20% CV 25% CV ** 25 500 bp: 15% CV 500 1,000 bp: 5% CV 100 1,000 bp: 10% CV 1,000 7,500 bp: 5% CV 100 1,000 bp: 15% CV 1,000 12,000 bp: 10% CV 0.1 50 ng/µl 0.5 50 ng/µl 0.5 50 ng/µl KCL NaCl 250 mm MgCl2 15 mm KCL NaCl 250 mm MgCl2 15 mm KCL NaCl 250 mm MgCl2 15 mm 35 30 30 12 / 12 / 12 / 1 µl 1 µl 1 µl 3 () 3 () 3 () * 70 bp ** Agilent Protein 80 Assay Agilent Protein 230 Assay 5 80 kda 14 230 kda 10% 10% * 10% CV (CA BLG) 10% CV (BSA CA ) * 3% CV (CA BLG) 3% CV (BSA CA ) (S/N > 3) 6 ng/µl CA (15 ng/µl BSA) PBS 10 ng/µl (CA ) 6 ng/µl CA (15 ng/µl BSA) PBS 30 ng/l (BSA) 0.5 M NaCl (30 ng/µl BSA 0.5 M NaCl ) 0.5 M NaCl 60 2,000 ng/µl CA PBS 15 2,000 ng/µl CA 30 2,000 ng/µl BSA PBS 6 4,000 ng/µl CA BLG 6 5,000 ng/µl CA 15 5,000 ng/µl BSA PBS 20% CV (CA BLG) 20% CV (BSA CA ) 30 25 10 / 10 / 4 µl 4 µl 3 () 3 () CA = BSA = BLG = * Agilent Assay 30 6/ 10 µl 4 C 3 11
Agilent 2100 10 kg (22 lbs) ( x x ) 290 162 412 mm (11.4 6.4 16.2 ) 100 240 VAC 50 60 Hz 60 VA 5 40 C IEC EN CSA UL 2 1 : www.agilent.com/chem/labonachip () www.agilent.com/chem/jp () : 0120-477-111 Copyright 2007 Agilent Technologies February 1, 2007 5989-6043JAJP