Key words: Vibrio cholerae, TCBS agar

Key words: Vibrio cholerae, TCBS agar

688 感 染 症学 雑 誌 Plate fig. 1 Colonies of KCP242 (K-4) strain V. cholerae on TCBS plates incubated at 37C. (This strain was isolated from the original strain (KC P242).) After 24 hrs 第53巻 第12号 び30 に お け る 発 育 状 況 を 観 察 し た. 成 1)形 態 学 的 試 験,生 績 化 学 的 性 状 試 験,生 物 型 の 決 定 試 験 及 び1血清 学 的 試 験 Tablel及 びTable2に 示 した ご と く,本 エ ル トー ル コ レ ラ菌 と 同 定 さ れ た.た コ レ ラ 菌 か ら は カ ヅ パ 型 フ ァ ー ジ3)は ず,非 溶 原 性 の コ レ ラ 菌 と 思 わ れ,他 菌は だ し,分 離 検 出 さ 才/ の菌 の カ ッ パ型 フー アジ に も この菌 は溶 菌 され ず 耐 性 で あつ た, 2)ペ プ ト ン水 に お け る 増 殖 実 験 Fig.1に 示 し た ご と く,pH7.2,pH8.6の ヘ フ トン 水 に お け る 各 菌 株 の 増 殖 曲 線 を み る と,pH 域 に よる影 響 は 各菌 株 に お いて ほ とん ど認 め られ After 36 hrs な か つ た.し か し,KCP242株,NIH35A3株 びMEl6株 の 増 殖 は 培 養 後9時 Fig. 48 間 に お い て,新 鮮 分 離 株 の そ れ と 比 較 し て 増 殖 が 悪 か つ た. 3)ロ After 及 ッ ト 番 号 の 異 な るTCBS培 1 The growth of V. cholerae ph 7.2 PH 8.6 hrs 地 上の 発 育 in peptone water

Table 1 Tests of morphology and biochemical activities The strains of KCP 242 (K-2) and KCP 242 (K-4) were isolated from the original strain(kcp 242). F: Fermentative

Table 2 Differentiation tests between V. cholerae and V. cholerae biotype El Tor and antiserum tests Table 3 Growth of V. cholerae strain on various TCBS agar (Incubated for 18 hrs at 37 Ž) Each strain was incubated in ph 7.2 peptone water for l6hrs at 37 Ž, and 0.1m1 of each dilution was inoculated into media. Numbers of organisms inoculated were 1.1 ~103 `6.9 ~103 at the 10-5 dilution.

Table 4 Growth of V. cholerae strain on various selective media (A) after 12 hrs (B) after 18 hrs (C) after 24 hrs *: Numbers of organisms inoculated werel.4 X 103 `5.3 x103 at the 10-5 dilution. The other remarks were same Table 3. (D) after 36 hrs

2) Miles, A. A., Misla, S. S. and Irwin, J.: Estimation of bactericidal power of the blood. J. Hyg., 38: 732-746, 1938. 6) John, P. and Graig, S.: Cholera in Guam. Symposium on cholera., 13-16, 1974.

A Strain of Vibrio cholerae Showing Undergrowth on TCBS Agar Hisao SAKAKIBARA, Iwao TANABE and Masami EMOTO Kobe Quarantine Station, Ministry of Health and Welfare, Kobe A strain of V. cholerae isolated from a cholera patient found in Kobe was proved to have the following charactristics: 1) The strain grew very slowly or was distinctly undergrown on TCBS agar which can well support the growth of V. cholerae organisms generally. It took 36-48 hrs before the formation of visible colonies (1-2 mm in diameter). At earlier stages of growth (about 18 hrs after the beginning of incubation at 37 Ž), the colonies had greenish tone, instead of yellowish tone as generally shown by the V. cholerae organisms. This was due to the lack of ability of decomposing sucroce. However, after 24 hrs of incubation, the colonies became yellowish. The same statement was made as to the results using peptone water. In general properties, this strain resembled those successively cultivated in peptone wa.ter. 2) Since there are V. cholerae strains which can grow very slowly on TCBS agar, it is absolutely necessary to use additionally other kinds of selective media, such as alkaline agar or Endo's agar, for the purpose of primary isolation of V. cholerae from natural sources. 3) Other characteristics such colony morphology, biochemical activities, sugar decomposition capacities and agglutinability against antiserum, etc. were essentially the same as those of typical V. cholerae. However, no Kappa type phage was isolated. Based on these data, we regarded this strain as classical El Tor vibrio of the continental type.