10 DDS New York Academy Renal Biochemistry (Elsevier) Glutathione (John Wiley & Sons) Antioxidants in Therapy and Preventive Medicine (Plenum) The Liver: Biology and Pathophysiology (Raven Press) NO SSC
Cu Zn Se A C E 2
1) 1) 1) 2) 3) 4) 1) 1) 1) 2) 3) 4) 1) ( )5 120 10 30 60 120 ( ) CR-300 135 ( ) RE-3305 2009 RVA X C A 1) 32 p.65-83 1985. Cu CuCl2 P 8 Cu CuCl2 1.0 mg% 30 60 90 120 min Cu 30 min 1/2 Cu CuCl2 Cu Cu
AIN93G 20 g/g Wistar 24 52 60 20 26 1, 2) 3) 2) 1) 2) 3) F344/N 3 17 AIN-93G 10 7 2 AIN-93G 7 Student t 5 3 1 3 1 4 1 A/G GOT 2 GPT ALP -GTP T3 T4 p = 0.069
3 ICR 1 kg 2 mg AIN-93G 1 AIN93G 10 HPLC Ca 1) 1) 1) 1) 1) 2) 2) 1) 1) 2) -3 FP FP FPH CHOL CHOL BA in vitro ] <> FP FPH 5 Wistar AIN93 50 FP FPH CHOL0.5% 0.1% 6 4 CHOL BA <In vitro > FP FPH CHOL BA FP FPH CHOL CHOL CHOL CHOL BA In vitro FP FPH BA CHOL FP BA CHOL FPH FP BA FP FPH CHOL CHOL BA BA FP FPH
Ca Mg Ca Mg AIN-93G AIN-93G Ca Mg 3 SD 4 9 14 23 28 Ca Mg Ca 9K(CBD9K) mrna Ca TRPV6 mrna Mg TRPM6 mrna Ca Ca Mg Mg Ca CBD9K mrna TRPV6 mrna TRPV6 mrna CBD9K mrna Mg Mg TRPM6 mrna TRPM6 mrna Ca Mg 1, 5) 2, 5) 3) 4) 1) ( ) 2) 3) 4) ( ) 5) 4, 5) TBT TBT Ca 2+ TBT TBT Ca 2+ 1. RCR-1 TBT 1 μm caspase-3 Propidium Iodide Ca 2+ TBT Ca 2+ Dulbecco s PBS (DPBS) BAPTA-AM 2. TBT Ca 2+ Ca 2+ Fura2-AM Ca 2+ calpain 3. JC-1 cytochrome c TBT RCR-1 caspase-3 Ca 2+ Ca 2+ calpain Ca 2+ BAPTA-AM Ca 2+ DPBS calpain cytochrome c TBT RCR-1 Ca 2+ 2
40 50 QOL PVEE PVEE HPLC Slc:ddY 9 OVX PVEE 20 mg/kg 8 Deoxyprydinoline DPD Tartarate Resistant Acid Phosphatase TRAP TRAP X CT TRAP mrna RT-PCR PVEE OVX TRAPmRNA DPD TRAP PVEE PVEE TRAP PVEE OVX PVEE 8 OVX PVEE PVEE 1) 2) 2) 2) 1) 2) ) Ca K Cu Mn Se medium chain fatty acids: MCT) Fe Zn Ca Cu BALB/C 40 1 10 C 3 1 50 100 150mL G-50 G-100 G-150 14 ICP-OES Fe Ca G-50 G-100 G-150
1) 1) 2) 1) 2) 1. 0.1mM NADH 2. NADPH NADPH 3. 0.1 0.5 mm 0.5 1 mm 2 mm 4. in vitro GSSG NADPH Ki 5 10 mm NAD(P)H
- 1) 1) 2) 1, 2) ArgRPseudomonas taetrolens NBRC 3460 D- 1) MALDI-TOF MS MALDI-TOF MS ArgR 2 A DTT B A B ArgR MALDI-TOF MS C47A/C73A-ArgR C47A/C73A-ArgR ArgR C47A/C73A-ArgR ArgR C47A/C73A-ArgR MALDI-TOF MS ArgR Cys47 Cys73 PLP ArgR ArgR 2 Cys Ala C47A/C73A-ArgR ArgR DTT Cys47-Cys73 ArgR ArgR Cys47-Cys73 ArgR 1) D. Matsui, T. Oikawa et al., (2009) Appl. Microbiol. Biotechnol., 83: 1045-1054. 1) 1) 2) 2) 1) 1) 2) K K1 K2 K3 4 K 4 SXR 4 K K1 K K K1 4 7 K K3 K K3 K K 58 mol/d K1 4 7 K3 (n = 4 5) 2 4 8 Sep-Pak Silica HPLC K K1 4 7 K1 7 2 4 K1 7 K3 4 K1 4 7 4 K 4 K3
- FAD FMN 2-2-CAA 2-2- 2-CAA 16S rrna 2-CAA 2-CAA 2-CAA Pseudomonas sp. YL 2-CAA 547 59,301 CAA67_YL CAA67_YL FAD 2-CAA H218O [18O] 2-CAA - 2--2-2- 2- FAD FADH2 CAA67_YL NAD(P)H AXXGXXG 205 211 NADH NADPH FAD NADH FAD CAA67_YL NADH FAD FADH2 2-1) 1) 1) 2) 2) 1) ( ) 2) 20 ppm 1 11 Wistar 220 g 2 1 1 4 2 2 1 mg /kg 0 4 3 9 Wistar 180 g 5 3 0 4 1 2 2 AUC 1 2 1 2 AUC
1) 2) 1) 2) (EPO) Biol Trace Elem Res 107: 289-292, 2005 HO-1 Sprague-Dawley 3 AIN-93G CONZn 4.5 mg/kg diet 4.5 ppm ZD Pair-Fed AIN-93G PF 3 10 4 HO-1 Ret Fisher PLSD 5 HO-1 CON ZD 3 Ret HO-1 CON ZD PF EPO EPO PF 21800073 Wistar 36 6 2 AIN93G 12.5 25 mg/kg 2 0.70 mg /ml 3.19 mg/ml 800 ml/kg 4 3 25 mg/kg 12.5 mg/kg 12.5 mg/kg
1, 4) 2) 3) 3) 4) 1) ( ) 2) 3) ( ) 4) TBT TBT 1. Wistar 6 170 g TBT 2.5 mg/kg body weight 24 96 CYH CYH 0.1 ppm 1 ppm 10 ppm 100 ppm TBT CYH 2. TBT 24 48 72 96 TBT GC/MS 3. Hematoxylin-Eosin HE TdT-mediated dutp-biotin Nick End Labeling (TUNEL) TBT CHY 100 96 0.1 10 ppm 50%GC/MS TBT TBT 24 TBT 24 96 TUNEL TBT 1) 2) 1) 1) 2) Rb Rb Rb Rb Rb Rb Rb Rb Rb 4 Wistar 62 8 Rb 0 4 8 25 50 100 200 1,000 ppm 46 Rb Williams 5% Rb Rb Rb 1 ppm Rb Rb 8 25 ppm 25 50 ppm 100 200 ppm Rb ppm Rb μg/g/ppm 0 4 6.6 4 8 7.8 8 25 4.4 25 50 10.7 50 100 12.9 100 200 7.8 200 1,000 6.1 Rb Rb 50 100 ppm Rb Rb50 100 ppm Rb50 100 ppm
4 26 1 50% 24 1 1 * 1) 2) 1) 1) 3) 1) 2) 3) 2000 1 400 μg 2006 2 5 45 33 7 29 400 μg/ 33 11 11 22 124 g 216 g 44.8% 41.4% 37.9% 27.6% 12 394 ± 113 μg 17 385 ± 103 μg 9 384 ± 120 μg 20 391 ± 102 μg 90% 400 μg 30%
1) 1) 2) 1) 2) 2010 50 μg/ 2 μg/ 5 μg/ 39 15 24 31.2 ± 4.0 FFQ L. plantarum ATCC 8014 50 100 50 4.5 N 4.5 N 100 μl 100 μl 37 18 610 nm 2001 2.5 ng/ml 2.9 ng/ml 2.8 ng/ml 2.3 ng/ml 18.8 μg/mgcre 24.3 μg/mgcre 13.2 μg/mgcre 14.6 μg/mgcre p < 0.05 36.5 μg 34.8 μg 35.2 μg 36.0 ± 7.2 μg/2010 1, 2) 1, 2) 2) 2) 1) 2) 75 10% 1) 60% 40% 2) (1) (1) Katayama M, Sugawa-Katayama,Y (2007) Effect of temperature on the diminution of retained arsenic in dried Hijiki, Sargassum fusiforme (harvey) Setchell, by water-soaking., J. Home Econ. Jpn, 58, 75-80. (2) Katayama M, Sugawa-Katayama Y, Yamaguchi Y, Murakami K, Hirata S (2008) Effect of temperature on the extraction of various arsenic compounds from dried Hijiki, Sargussum fusiforme by water-soaking as a pre-cooking process. Trace Nutrients Research 25: 134-138.
Se- GC-MS Se- - -Se- LC-MS g/g 125 45.685.2 0.1 g 0.1 ml 0.45 m LC-MS Applied Biosystems, API 3000 Cadenza CD-C18 Imtact/70/30 0 10/90 40 0.2 ml/min Inertsil ODS-3 GL Sciences /80/30 0 10/90 45 0.2 ml/min - -Se- LC-MS LC Se- - -Se- Se- - -Se- LC-MS 1) 2) 3) 4) 1) 1) 1) 2) 3) 4) 2 70 1998 1998 2008 10 2005 2 157 137 2005 77 78 NaKCa Mg P S ZnFe Cu Ca Na Zn Fe 40
1) 1) 2) 2) 3) 3) 1) 1, 2) 1) 1) 2) 3) 1, 2) 22.1 20 40 Klotho Klotho KO Klotho Klotho 500 g 550 g SD F 40 calorie restriction CR 2 1 4 Klotho real-time PCR Klotho SDS-PAGE F 511.4 ± 13.7 g 556.0 ± 15.5 g F 60 CR 529.3 ± 17.2 g 470.3 ± 9.8 g Real-time PCR Klotho m-rna F CR 1.5 SDS-PAGE Klotho F CR Klotho