STEM CELL PORTFOLIO driving breakthroughs in research through innovations in process
10 Go to lifetechnologies.com/stemcell 2
Contents 4 8 9 10 16 18 21 26 10 11 12 14 ECM Culture Differentiate Engineer Analyze 3
KnockOut Serum Replacement (SR) ESC/iPSC KnockOut SR300 ESC/iPSC KnockOut SR ESC/iPSCFBS KnockOut SRKnockOut DMEM KnockOut DMEM/F-12 1 FBS Go to www.invitrogen.com/ksr KnockOut SR XenoFree CTS ESC/iPSC xeno-free KnockOut SR XenoFreeKnockOut SRhESC/hiPSC xeno-freehesc CELLstart CTS KnockOut SR GF Cocktail 2 Go to www.invitrogen.com/ksrxf 1. ESC/iPSC 100 DMEM Culture system Basal media Complete media Supplements Feeders and extracellular matrices KnockOut DMEM Human ESC/iPSC ESC 80 60 40 20 0 ESC FBS KnockOut Serum Replacement Serum + feeders Serum-free + feeders Serum-free + feeder-free Xeno-free + feeders (human) Xeno-free + feeder-free Mouse ESC/iPSC KnockOut DMEM KnockOut DMEM/F-12 KnockOut DMEM KnockOut DMEM/F-12 KnockOut DMEM KnockOut DMEM StemPro hesc SFM ESC-Qualified FBS KnockOut SR KnockOut SR XenoFree KnockOut SR XenoFree + KnockOut SR Growth Factor Cocktail Mouse embryonic fibroblasts Mouse embryonic fibroblasts Geltrex hesc-qualified/ CELLstart CTS Human foreskin fibroblasts CELLstart CTS CELLstart CTS 1. ESC ESC FBS KnockOut SR DMEM KnockOut DMEM D3 ESC LIF 7 ESC Serum + feeders Serum-free + feeders KnockOut DMEM DMEM/F-12 KnockOut DMEM ESC-Qualified FBS KnockOut SR Mouse embryonic fibroblasts Mouse embryonic fibroblasts 4
KnockOut SR Growth Factor Cocktail ESC/iPSC KnockOut SR Growth Factor Cocktail GFC ESC/iPSC ESC/iPSC2 KnockOut SR KnockOut SR XenoFree CTS ESC/iPSC KnockOut DMEM ESC/iPSC KnockOut DMEM ESC ipsc DMEM ESC/iPSCKnockOut SR KnockOut SR XenoFree CTS ESC FBS L-L- GlutaMAX Go to www.invitrogen.com/knockout KnockOut DMEM/F-12 ESC/iPSC KnockOut DMEM/F-12 DMEM/F-12ESC ipsc ESC/iPSC hesc L- KnockOut SR MEF Kit and Gibco Mouse Embryonic Fibroblasts (MEFs) ESC/iPSC Gibco Mouse Embryonic Fibroblast MEF KnockOut Serum Replacement ICR 13.5 E13.5 2P2 80% Go to www.invitrogen.com/mef A B C 2. KnockOut SR Growth Factor Cocktail ips ipscgeltrex 20% KnockOut SR KnockOut SR Growth Factor Cocktail 2 mg/mla 40B DAPI 100C SSEA4 100 KnockOut SR XenoFree CELLstart CTS xeno-free substrate KnockOut SR GF Cocktail ipsc ipsc 5 5
StemPro hesc SFM hesc/hipsc StemPro hesc SFMhESC/hiPSC 3StemPro hesc SFMGeltrex RGF Matrix Geltrex hesc-qualified CELLstart CTS hesc/ipsc Go to www.invitrogen.com/stempro/hesc MesenPRO RS Medium MSC2% MesenPRO RS DMEM + 10% FBS MSC 2% FBS10 20% FBS Go to www.invitrogen.com/mesenpro StemPro MSC Serum Free Medium (SFM) MSC StemPro MSC SFM MSC DMEM + 10% FBS p63 CFU-F 6 MSC Go to www.invitrogen.com/stempro/msc StemPro MSC SFM XenoFree MSC xeno-free StemPro MSC SFM XenoFree MSC ADSCDMEM + 10% MSC FBSStemPro MSC SFM XenoFree CELLstart Substrate 6 MSC Go to www.invitrogen.com/mscxenofreeee 3. StemPro hesc SFM hesc StemPro hesc SFMhESC3 StemPro hesc SFM25 BG02 6
StemPro LipoMAX Supplement xeno-free StemPro LipoMAX StemPro LipoMAX ADSC 3 50% MSCADSC ADSC Go to www.invitrogen.com/stemcell/msc Synth-a-Freeze Medium Synth-a-Freeze 10%DMSO Synth-a-Freeze 1 MSC ESC ipsc Synth-a-Freeze StemPro NSC SFM StemPro NSC SFMhNSC 4 NSC NSC StemPro NSC SFMNSC Go to www.invitrogen.com/stempro/nsc Neurobasal and Neurobasal -A Media Neurobasal Medium Neurobasal -A Medium Go to www.invitrogen.com/neurobasal StemPro -34 SFM CD34+ SFM StemPro -34 SFM StemPro -34 SFM Go to www.invitrogen.com/stempro34 4. StemPro NSC SFM 7
StemPro EZPassage Tool StemPro EZPassage 1 hesc/hipsc 5 611 Go to www.invitrogen.com/stempro/ezpassage to watch the StemPro EZPassage Tool video. 5. StemPro EZPassage hesc/hipscstempro EZPassage Gibco TrypLE Select CTS Invitrogen TrypLE Select CTS Go to www.invitrogen.com/gibcocts StemPro Accutase StemPro Accutase heschnsc 6 hesc Go to www.invitrogen.com/accutase A B 6. StemPro Accutase 1 ml 10 A B 8
Alexa 594-A Alexa 594-A Dynabeads SSEA-4 Dynabeads SSEA-4SSEA-4+ ESC 7 hesc Go to www.invitrogen.com/dynal Dynal CD34 Progenitor Cell Selection System CD34+ CD34+ DC Dynabeads in vitro Dynabeads ISO 9001:2008 Dynabeads CTS Class 100 Dynabeads cgmp Dynabeads CTS ISO 13485:2003 Dynabeads Dynabeads Invitrogen Dynal Dynabeads ISO 9001:2008 ISO 13485:2003 21 CFR 820 US cgmp DynaMag CTS Dynabeads Dynabeads CD3/CD28 CTS T 10 5 10 5 10 4 10 4 10 3 10 3 10 2 10 2 10 1 10 1 10 0 50 100 150 200 250 FSC-A (x 1,000) 10 0 50 100 150 200 250 FSC-A (x 1,000) 7. hesc BG01 Dynabeads SSEA-4 SSEA-4 9
in vitro HDFa HDFn HEKa HEKn HEMa HEMn Go to www.invitrogen.com/primarycells Gibco Human Neural Stem Cells (H9-Derived) StemPro NSC SFM StemPro Human Adipose-Derived Stem Cell Kit ADSC ADSC ADSC MesenPRO RS Gibco Rat Fetal Neural Stem Cells NSC 75% Gibco Mouse (C57BL 6) Mesenchymal Stem Cells 8C57BL/6 Gibco Rat (SD) Mesenchymal Stem Cells 8Sprague-Dawley SD StemPro Rat Alk Phos Expressing Mesenchymal Stem Cells ROSA26 Fisher 3448 Go to www.invitrogen.com/stemcell/msc 8. ELF 97 Endogenous Phosphatase Kit StemPro Rat Alk Phos Expressing Mesenchymal Stem Cell 10
Geltrex hesc-qualified Geltrex hesc-qualifiedstempro hesc SFM hescpcr Geltrex hesc-qualified hesc/hipsc CELLstart CTS xeno-free CELLstart CTS xeno-free CELLstart CTS hesc/hipschmschnsc 9 hesc/hipsc Children s Memorial Research Center E. Seftor CELLstart CTS StemPro hesc SFMhESCH9 60 10 Go to www.invitrogen.com/3d-cellculture Natural Mouse Laminin A B 9. MSCCELLstart CTS 4 4 MSC CELLstart CTS StemPro MSC SFM XenoFree 9 B A 11
StemPro Accutase TrypLE Select CTS TrypLE Express StemPro Accutase TrypLE TrypLE ESC ipsc NSC TrypLE Select CTS TrypLE Express TrypLE Select CTS PSC MSC NSC HSC HSC 100 ml 100 ml 100 ml 500 ml 5 L 12
Dispase Collagenase IV StemPro EZPassage ESC ipsc Clostridium histolyticumpro-x-glyc- ProX StemPro EZPassage ESC ipsc 5 g 1 g 10 13
ECM 1998ESC Gibco Gibco ECMin vitro Laminin Fibronectin Collagen Geltrex Geltrex Engelbreth-Holm-Swarm ECM EHS Engelbreth-Holm- hesc/hipsc Swarm EHS Matrigel ESC ipsc Geltrex NSC PSC MSC NSC HSC 1 mg I 10 ml I 20 ml24 hesc LDEV 14
CELLstart CTS AlgiMatrix Gelatin Vitronectin CELLstart CTS AlgiMatrix xeno- 3 free CELLstart CTS 0.1% Recombinant Vitronectin hesc hipsc hmsc 1 Natural Human hnsc MEF AlgiMatrix MEF 2 ml 624 96 100 ml 100 mg 15
Gibco Gibco Gibco FGF-basic Full Length CTS LIF BMP-4 SCF CTS BDNF EPO IL-2 IL-4 IL-7 CTS TNF- CTS Go to www.invitrogen.com/growthfactors B-27 Supplement B-27 Supplement50 Gibco B-27 Supplement xeno-free N-2 Supplement N-2 SupplementPNS CNS G-5 Supplement G-5 Supplement Neurobasal Go to www.invitrogen.com/stemcell/nsc Gibco MSC StemPro MSC SFM MesenPRO RS MSC FBS cgmp PCR StemPro Adipogenesis Differentiation Kit 10 StemPro Chondrogenesis Differentiation Kit StemPro Osteogenesis Differentiation Kit Go to www.invitrogen.com/stempro/mscdiff 10. O 16
Gibco Gibco Cell Therapy Systems (CTS ) Gibco Cell Therapy Systems CTS 21 CFR Part 820 Quality System RegulationISO 13485/ISO 9001 Gibco Gibco 2 cgmp cgmpgibco Cell Culture Custom Media Services10,000 L 1,000 L Advanced Granulation Technology AGT 7,500 kg Gibco MediaExpress SM cgmp Gibco cgmp SDS-PAGE >95% SDS-PAGE ED 50 PD-Direct PD-Direct Bioprocess Services LAL Limulus Amoebocyte Lysate <0.1 g/mg 2. Gibco Gibco cgmp cgmp Gibco I21 CFR Part 864, subpart CcGMP cgmp Custom Products Services 17
Jump-In TI expression Jump-In TI Gateway R4 attp GOI 1 20 GPCR Go to www.invitrogen.com/jumpin Gateway Cloning Gateway Gateway 1 99% 1 DNA Go to www.invitrogen.com/gateway Jump-In TI Gateway 21R4 attpjump-in TI 2R4R4 attp >95% 11 R4 attp Zeocin SV40 pa PhiC31 attb Ampicilin UMS HSV TK pjti /Zeo 6597 bp Hygromycin Phi C31 Integrase R4 att P Step I UMS Zeo TK-Hyg puc ori Amp puc ori HSV TKPA Amplicilin attr2 ccdb Cm R attr1 puc ori pjti R4 DEST 5583 bp EF1a Multisite Gateway cloning GOI puc ori Expression construct EF1a R4 att B Step II Retargeting (+) R4 integrase R4 attb R4 att R Ampicilin UMS Amp puc ori GOI EF1a Zeo TK-Hyg puc ori Amp R4 att L 11. Jump-In TI HEK 293 CHO-S I1 phic31 2 Hyg3456a Hyg qpcrb II7R4 8 Zeo/G418/Bsd 9 18
Lentiviral ipsc Reprogramming Particles ipsc hoct4 hsox2 hklf4 hc-myc hlin28 hnanog CMV-GFP hoct4-gfpipsc Go to www.invitrogen.com/lenti Neon Transfection System DNA sirna 80% Neon Transfection 12 Neon Transfection 3 24 Go to www.invitrogen.com/neon pebna DEST Vector Kit pebna-destmultisite Gateway Protocol Exchange protocolexchange.community.invitrogen.com Epstein-BarroriP EBNA-1 B Go to www.invitrogen.com/vectors BacMam Technology BacMam Go to www.invitrogen.com/bacmam 12. Neon Transfection % % ES 53 97 54 90 88 96 88 96 H9 29 99 3. Neon Transfection 19
BacMam EBNA ASC StemPro TARGET hesc BG01V Kit Jump-In TI Gateway BG01V 13 3 14 Pseudo attp 3 PhiC31 pjti/zeo Hyg ptk Zeo R4-attP R4 pjti/eg ptk pef1a pef11 SMA AFP Hyg Zeo GFP R4-attL R4-attR 13. BG01VR4 phic31 StemPro TARGET BG01V Zeocin R4 attp R4 attb EF1a MultiSite Gateway DESTR4 TARGET R4 attb attp Zeocin EF1a Zeocin MultiSite Gateway DEST StemPro BG01V/EG EmGFP 14. EF1a-EmGFP StemPro TARGET BG01V 22 GFP3 GFP 20
Alkaline Phosphatase Live Stain (AP Live Stain) ESiPS Go to lifetechnologies.com/aplivestain Cell Line Contamination TaqMan PCR Go to www.appliedbiosystems.com/cell-contamination Genomic Instability DNA STR SNP DNA Gene Expression TaqMan mrna 4 ml1 PCR 1 TaqMan PCR1 TaqMan Human Mouse Stem Cell Pluripotency Assay 1 96 384 TaqMan Array 384 TaqMan ArrayApplied Biosystems 7900HT ViiA 7 PCR Go to www.appliedbiosystems.com/gene-expression Go to www.appliedbiosystems.com/genomic-instability 15. AP Live Stain MEFH9 21
Protein Expression FACS Go to www.appliedbiosystems.com/protein-expression Western-SuperStar Immunodetection System Western-SuperStar Immunodetection Western-SuperStar sirna TaqMan Protein Assays TaqMan Protein Expression Assay TaqMan 5TaqMan Gene Expression MicroRNA (mirna) Assay mrna mirna16 TaqMan Protein Assays Open Kit TaqMan Protein Assay Go to www.appliedbiosystems.com/taqman Cell Lysis Protein Expression Sample Preparation Kit Binding TaqMan Protein Assay Kit 3 oligo 5 oligo connector epitope 1 Target epitope 2 Target 60 min 10 min 15 min TaqMan Fast Real-Time PCR Applied Bios ystems Fast Real-Time PCR System Data Analysis TaqMan Protein Assay Data Analysis Software v1.0 (beta1) Fluorescence High concentration Intermediate concentration Low concentration Background Fold change 100 10 1 0.1 Cycle number 0.01 1 4 10 Day 14 28 Final 45 min 16. TaqMan Protein Assay 22
Qtracker Labels Qtracker Cell Labeling Kit Qdot Qtracker Go to www.invitrogen.com/qdots Alexa Fluor Dyes Alexa Fluor 30,000 Alexa Fluor 18 Go to www.invitrogen.com/alexa PrestoBlue Cell Viability Reagent 10 PrestoBlue 10PrestoBlue 2 1 4 3 5 6 8 7 9 10 11 12 13 14 15 16 17 18 10 17 Z Go to www.invitrogen.com/prestoblue A B 400 450 500 550 600 650 700 750 800 850 1. Alexa Fluor 350 2. Alexa Fluor 405 3. Alexa Fluor 430 4. Alexa Fluor 488 5. Alexa Fluor 514 6. Alexa Fluor 532 7. Alexa Fluor 546 8. Alexa Fluor 555 9. Alexa Fluor 568 10. Alexa Fluor 594 11. Alexa Fluor 610 12. Alexa Fluor 633 13. Alexa Fluor 647 14. Alexa Fluor 660 15. Alexa Fluor 680 16. Alexa Fluor 700 17. Alexa Fluor 750 18. Alexa Fluor 790 18. Alexa Fluor 17. Geltrex 96H9 AB PrestoBlue 23
Click-iT EdU Cell Proliferation Assay Kits Click-iT EdU DNAClick-iT EdU Click-iT EdUEdU 19 BrdU DNA Go to www.invitrogen.com/clickitedu Premo Autophagy Sensors ready-to-use CellLight Premo Autophagy Sensor 20 Premo FUCCI Cell Cycle Indicator Premo FUCCI Cell Cycle Sensor FUCCI BacMam CellLight Reagents CellLight 21 CellLight BacMam BacMamBacMam 19. DNA Click-iT EdU Alexa Fluor 647 Imaging Kit Alexa Fluor 488Hoechst 33342 20. Cascade Biologics HASMC Premo Autophagy Sensor LC3B-GFP CellLight Golgi-RFP Hoechst 33342 21. CellLight CellLight Histone 2B-RFP CellLight MAP4-GFP 24
RNAi Gene Regulation with TaqMan mirna Assays microrna mirnamirna RNA mrna mrnamrnamirna Go to www.appliedbiosystems.com/gene-regulation Gene Knockdown with sirna sirna Go to www.appliedbiosystems.com/gene-knockdown Epigenetic Monitoring methylseqr Bisulfite Conversion KitPCR Go to www.appliedbiosystems.com/epigenetic-monitoring Applied Biosystems Attune Acoustic Focusing Cytometer Attune Acoustic Focusing Cytometer Attune Acoustic Focusing Cytometer1 22Attune Cytometer Go to www.appliedbiosystems.com/attune 22. Attune Acoustic Focusing Cytometer 25
Ordering Information ESC ipsc CELLstart CTS A1014201 2 ml Collagenase Type IV 17104-019 1 g Dispase 17105041 5 g DMEM 11885-084 500 ml DMEM/F-12 10565-018 500 ml ESC Qualified FBS 10439024 500 ml FGF-Basic (recombinant human) 13256029 10 µg Geltrex hesc-qualified A1048002 5 ml Geltrex RGF Matrix 12760021 5 ml KnockOut DMEM 10829018 500 ml KnockOut DMEM/F-12 12660012 500 ml KnockOut SR 10828028 500 ml KnockOut SR GF Cocktail A1058001 10 ml KnockOut SR XenoFree A1099202 500 ml LIF (recombinant human) PHC9461 100 µg StemPro Accutase A1110501 100 ml StemPro hesc SFM A1000701 1 kit StemPro EZPassage Tool 23181010 10 TrypLE Express with Phenol Red 12605-010 100 ml Trypsin-EDTA 15400-054 100 ml B-27 Serum-Free Supplement (50X) 17504-044 10 ml B-27 Supplement Minus AO (50X) 10889-038 10 ml B-27 Supplement Minus Insulin (50X), liquid (Custom) 0050129SA 10 ml B-27 Supplement Minus Vitamin A (50X) 12587-010 10 ml B-27 Supplement XenoFree (50X) A11576-SA 10 ml N-2 Supplement (100X), liquid 17502-048 5 ml G-5 Supplement (100X), liquid 17503-012 1 ml FGF-basic (recombinant human) 13256-029 10 µg Geltrex hesc-qualified A1048002 5 ml Geltrex RGF Matrix 12760021 5 ml KnockOut DMEM 10829018 500 ml KnockOut DMEM/F-12 12660012 500 ml KnockOut SR 10828028 500 ml KnockOut SR GF Cocktail A1058001 10 ml KnockOut SR XenoFree A1099202 500 ml MesenPRO RS Medium 12746-012 1 kit Neurobasal -A Medium 10888-022 500 ml Neurobasal -A Medium, without Phenol Red 12349-015 500 ml Neurobasal Medium 21103-049 500 ml Neurobasal Medium, without Phenol Red 12348-017 500 ml StemPro Adipogenesis Differentiation Kit A10070-01 1 kit StemPro Chondrogenesis Differentiation Kit A10071-01 1 kit StemPro Osteogenesis Differentiation Kit A10072-01 1 kit StemPro hesc SFM A1000701 1 kit StemPro LipoMAX A10850-01 5 ml StemPro MSC-SFM A10332-01 1 kit StemPro MSC-SFM XenoFree A10675-01 1 kit StemPro NSC-SFM A10509-01 1 kit StemPro -34 SFM 10639-011 1 kit StemPro Human Adipose Derived Stem Cells R7788-115 1 vial StemPro Human Adipose Derived Stem Cell Kit R7788-110 1 kit Synth-a-Freeze Medium A12542-01 50 ml Mouse (C57BL 6) Mesenchymal Stem Cells S1502-100 1 ml Rat (SD) Mesenchymal Stem Cells S1601-100 1 ml Rat Fetal Neural Stem Cells N7744-100 1 ml Rat Fetal Neural Stem Cell Kit N7744-200 1 kit Human Neural Stem Cells (H9-Derived) N7800-100 1 ml Human Neural Stem Cell Kit (H9-Derived) N7800-200 1 kit StemPro Rat Alk Phos Expressing Mesenchymal Stem Cells R7789-120 1 ml StemPro Human Adipose Derived Stem Cells R7788-115 1 vial StemPro Human Adipose Derived Stem Cell Kit R7788-110 1 kit AlgiMatrix 3D Culture System 6-well (+ 50mL Firming Buffer) A10982-01 1 plate AlgiMatrix 3D Culture System 6-well (+ 50mL Firming Buffer) A10982-02 4 plates AlgiMatrix 3D Culture System 24-well 12684-023 1 plate AlgiMatrix 3D Culture System 24-well 12684-049 4 plates AlgiMatrix 3D Culture System 96-well plate 12684-015 1 plate AlgiMatrix 3D Culture System 96-well plate 12684-031 5 plates AlgiMatrix Firming Buffer A10915-01 50 ml AlgiMatrix Dissolving Buffer A11340-01 50 ml Attachment Factor(Gelatin) S-006-100 100 ml CELLstart CTS A1014201 2 ml Collagen I, Bovine A10644-01 10 ml Collagen I, Rat tail A10483-01 20 ml Fibronectin Human, Plasma 33016-015 5 mg Fibronectin Natural Human PHE0023 1 mg Fibronectin, Bovine Plasma 33010-018 1 mg Geltrex hesc-qualified A1048002 5 ml Geltrex LDEV Free RGF Matrix A1134301 5 ml Geltrex RGF Matrix 12760021 5 ml Natural Mouse Laminin 23017-015 1 mg GIBCO recombinant growth factors Activin A (human) PHG9014 5 µg Activin R1b (human) PHC9284 10 µg Angiopoietin-2 (human) PHC1524 10 µg B-DNF (human) PHC7074 10 µg BMP-2 (human) PHC7145 10 µg BMP-4 (human) PHC7914 5 µg BMP-7 (active) (human) PHC7204 10 µg BMP-7 (inactive) (human) PHC7104 10 µg Brain-Derived Neurotrophic Factor (BDNF) (human) 10908-010 5 µg CNTF (human) PHC7015 20 µg Cripto (human) PHC8014 10 µg CTGF (human) PHG0286 20 µg EGF (human) PHG0315 25 µg EGF (Murine, Natural) 53003-018 100 µg EPO (human) PHC2054 500 IU FGF-10 (human) PHG0204 25 µg FGF-16 (human) PHG0214 25 µg FGF-17 (human) PHG0224 25 µg FGF-18 (human) PHG0234 25 µg FGF-19 (human) PHG0244 25 µg FGF-4 (human) PHG0154 25 µg FGF-6 (human) PHG0174 25 µg FGF-7 (KGF) (human) PHG0094 10 µg FGF-8 (human) PHG0184 25 µg FGF-8b (human) PHG0274 10 µg FGF-9 (human) PHG0194 20 µg FGF-basic (full length) (human) PHG0264 10 µg FGF-basic (full length) (human) PHG0266 25 µg FGF-basic (full length) (human) PHG0261 100 µg FGF-basic (full length) (human) PHG0263 1 mg FGF-basic (human) 13256-029 10 µg FGF-basic, AA 10-155 (human) PHG0024 10 µg FGF-basic, AA 10-155 (human) PHG0026 50 µg FGF-basic, AA 10-155 (human) PHG0021 100 µg FGF-basic, AA 10-155 (human) PHG0023 1 mg Flt-3L (human) PHC9414 10 µg GDNF (human) PHC7045 10 µg IL-10 (human) PHC0105 10 µg IL-2 CTS (human) CTP0021 100 µg IL-4 CTS (human) CTP0041 100 µg IL-6 (human) PHC0065 10 µg IL-7 CTS (human) CTP0071 100 µg LIF (human) PHC9464 10 µg 26
LIF (human) PHC9461 100 µg Midkine (human) PHC7084 20 µg Neurturin (human) PHC7064 10 µg NT-4 (human) PHC7024 5 µg Oncostatin M (human) PHC5015 10 µg SCF c-kit Ligand (human) PHC2116 25 µg SDF-1a (human) PHC1344 10 µg Sonic Hedgehog C25II (mouse) PMC8034 10 µg TGF- (human) PHG0051 100 µg TGF- 1 (human) PHG9204 5 µg TGF- 2 (human) PHG9114 5 µg TNF- CTS (human) CTP3011 100 µg Wnt-1 (human) PHC1804 10 µg StemPro Accutase A11105-01 100 ml StemPro EZPassage Tool 23181-010 10 TrypLE Express with Phenol Red 12605-010 100 ml TrypLE Select CTS A12859-01 100 ml Trypsin-EDTA 15400-054 100 ml Dynabeads SSEA-4* * 111-60D 1 kit DynaMag CTS 12102 1 Human Dermal Fibroblasts, adult (HDFa) C-013-5C 1 vial Human Dermal Fibroblasts, neonatal (HDFn) C-004-5C 1 vial Human Epidermal Keratinocytes, adult (HEKa) C-005-5C 1 vial Human Epidermal Keratinocytes, neonatal (HEKn) C-001-5C 1 vial Medium 106 M-106-500 500 ml EpiLife Medium M-EPI- 500-CA 500 ml EpiLife Defined Growth Supplement (EDGS) S-012-5 5 ml, 100 X Human Keratinocyte Growth Supplement (HKGS) S-001-5 5 ml, 100 X Low Serum Growth Supplement (LSGS) S-003-10 10 ml, 50 X Supplement S7 S-0175 5 ml Keratinocyte AOF Growth Kit A1051501 1 kit Neon Transfection System Neon Transfection System Starter Pack MPK5000S 1 Neon Transfection System MPK5000 1 Neon Transfection System Kits Neon Transfection System Kit (100 µl) MPK10096 1 kit Neon Transfection System Kit (10 µl) MPK1096 1 kit Neon Transfection System accessories Neon Transfection System Pipette MPP100 1 Neon Transfection System Pipette Station MPS100 1 Neon Transfection Tubes 100 MPT100 1 Secondary antibody conjugates (selected) Host Label donkey anti-goat IgG (H+L) A11055 Alexa Fluor 488 goat anti-mouse IgG (H+L) A11001 Alexa Fluor 488 goat anti-mouse IgM (µ chain) A21042 Alexa Fluor 488 goat anti-rabbit IgG (H+L) A11008 Alexa Fluor 488 goat anti-rat IgG (H+L) A11006 Alexa Fluor 488 rabbit anti-mouse IgG (H+L) A11059 Alexa Fluor 488 Qtracker Cell Labeling Kits Qtracker 525 Cell Labeling Kit Q25041MP 1 kit Qtracker 565 Cell Labeling Kit Q25031MP 1 kit Qtracker 585 Cell Labeling Kit Q25011MP 1 kit Qtracker 605 Cell Labeling Kit Q25001MP 1 kit Qtracker 655 Cell Labeling Kit Q25021MP 1 kit Qtracker 705 Cell Labeling Kit Q25061MP 1 kit Qtracker 800 Cell Labeling Kit Q25071MP 1 kit PrestoBlue Cell Viability Reagent A13261 25 ml PrestoBlue Cell Viability Reagent A13262 100 ml Jump-In TI Platform A10897 1 kit Jump-In TI Gateway A10896 1 kit Jump-In TI Gateway A10895 20 pebna DEST Gateway A10898 1 kit ipsc Lentiviral ipsc Reprogramming Particles HOCT4 A1357101 100 ul Lentiviral ipsc Reprogramming Particles HSOX2 A1357201 100 ul Lentiviral ipsc Reprogramming Particles HKLF4 A1357301 100 ul Lentiviral ipsc Reprogramming Particles HC-MYC A1357401 100 ul Lentiviral ipsc Reprogramming Particles HNANOG A1357501 100 ul Lentiviral ipsc Reprogramming Particles HLIN28 A1357601 100 ul Lentiviral ipsc Reprogramming Particles CMV-GFP REPORTER A1357701 100 ul Lentiviral ipsc Reprogramming Particles OCT4-GFP REPORTER A1357801 100 ul 27
2012 Life Technologies Japan Ltd.All rights reserved.the trademarks mentioned herein are the property of Life Technologies Corporation or their respecitve owners. B-27 is a registered trademark of Southern Illinois University. Taqman is a registered trademark of Roche Molecular Systems,Inc. For research use only. Not intended for any animal or human therapeutic or diagnostic use. 108-0023 4-2-8 www.lifetechnologies.com 564-0052 10-28 GIB024-B1203OB