Yeast Two-Hybrid System Kit N-G Wako & TranSort

Yeast Two-Hybrid System Kit N-G Wako & TranSort

l (small unilamellar vesicle : SUV) DNA l l l l l l l 12ml35mm 110 5 21824 (4080 ) 37CO2 (5) 3 DNA0.252g50l DNA 440l2.520l 3.DNA 15DNA- l l l l l l 522mlPBS1.9ml 6DNA-24 37CO2 (5) 72mlPBS 8 50400 (35mm) Total lipids2mol Obika, S., Yu, W., Shimoyama, A., Uneda, T., Miyashita, K., Doi, T. and Imanishi, T. : Bioorg. & Med. Chem. Lett., 7, 1817 (1997) l (TMAG) DNA DNADNA DNA (35mm40) 1) Nobuyuki, H. and Kunio, Y. : J. Clin. Biochem. Nutr., 7, 175 (1989) 2) Mebio,26 (1992) 3) 4-108391 4) 12No.15 () 57 (1994) 5) 12 (No.13), 77 (1994) 6) 14 (No.4), 85 (1996) 7) Endoh, D. et al. : Jpn. J. Vet. Res., 43, 109 (1995)

(DOGS) DNA DNA 50 1) Jean-Paul Behr, et al. : Proc. Natl. Acad. Sci. USA., 86, 6982 (1989) 2) Corman, C. M. et al. : Mol. Cell. Biol., 2, 1044 (1982) 3) Chen, C. and Okayama, H. : Mol. Cell. Biol., 7, 2745 (1987) 4) Tsukamoto, M. et al. : Nature Genetics, 9, 243 (1995) l in vivoin vitro in vivo 100200 1) Otmane, B., Frank, L. et al. : Proc. Natl. Acad. Sci. USA., 92, 7297 (1995) 2) Regi, L., Yves, M. et al. : Molecular and Cellular Neuroscience, 7, 239 (1996) 3) Noguiezhellin, P. et al. : Proc. Natl. Acad. Sci. USA, 93, 4175 (1996) 4) Boussif, O. et al. : Gene Therapy, 3, 1076 (1996) DNA Calf thymus 1) 12, 184 (1994) 2) BIOTHRAPY, 8, 1265 (1994) 3) Kaneda, Y., Iwai, K. and Uchida, T. : J. Biol. Chem., 264, 12126 (1989) 4) Kaneda, Y., Iwai, K. and Uchida, T. : Science, 243, 375 (1989) 5) Kaneda, Y., Morishita, R. and Tomita, N. : J. Molec. Medicine, 73, 289 (1995) 1mgml 6) Morishita, R. et al. : Proc. Natl. Acad. Sci. USA, 92, 5855 (1995) 7) Yoshida, M. et al. : Proc. Natl. Acad. Sci. USA, 92, 9570 (1995) 8) Tomita, N. et al. : Gene Therapy, 3, 477 (1996) 9) Isaka, Y. et al. : Nature Medicine, 2, 418 (1996) 10) (), 104 (1996)

l DNA, RNASYBR Green DNA, RNA1,000 DNARNA 300nm10 SYBR Green 0.6 (DNA, RNA) glyoxalated RNA25100 SSCPSYBR Green ex300nm495nm em537nm 500l (DMSO) 10,000 ( S-7569) Extractor TM 1 l 1 l 2 l l 203010 200pgM13mp19RF DNA 1,000 (DMSO) 100 ex475nm ( 254nm) em499nm 300nm 254nm SYBR DX SYBR GreenGold (S-7569)

l 200A260 RiboGreen TM RNA Quantitation Reagent 1ml 1,000 20TE 25ml 1ngml1gml Ribosomal RNA Standard (100gml) 5200l ATP ex480nmem520nm 200l 200pg RiboGreen TM DNA DNaseRNA l 2ml100pgml ssdna A26010,000 100pgml1gml ATP DNARNA OliGreen ssdna Quantitation Reagent 20TE Oligonucleotide Standard (100gml) 200 1ml 25ml 1ml 200l 1ngml l l l

DupLEX-A TM FieldSong 1) Gal4p two-hybrid LexA Two-Hybrid- (Bait) (Target) DupLEX-A TM DNA LexA Acid BlobB42LexA LexA B42 B42 false positive B42 Bait B42 HA tagbaitpotential positive Bait Bait BaitLex A l 1) Fields, S. and Song, O. : Nature, 340, 245 (1989) 2) Chien, C. T. et al. : Proc. Natl. Acad. Sci. USA, 88, 9578 (1991) 3) Gyuns, J. et al. : Cell, 75, 791 (1993) 535-53451 (DKT-100) DupLEX-A Yeast Two-Hybrid System Kit 1kit 88,000

Java TM Web Macintosh WindowsUNIXWeb Protein Adviser () Epitope Adviser ()

mrna5' cdna 1) primer extension S1 nuclease mapping5'race () PCR primer extensions1 nuclease mapping mrna Cap Site cdna TM 1RC2TGP1 5'RACE 1st PCR2RC2TGP2 5' Cap Site cdna TM mrna5',, nested PCR nested PCR DNA 1st PCR DNA (Cap Site cdna TM ) Cap Site cdna TM, Mouse Testis-actin Cap Site Huntingcontrol primercp1cp2tgp1tgp2 nested PCR 2) ( 80) (3 ) 3 -actin -actin-actin -actin-actin-actin nested PCR 3 -actin-actin 3) 4) -actin 74-actin73 5) Cap Site cdna TM Cap Site Hunting mrna 5'

1 for 7days in 16hr light and 8hr darkness per day 2 for 7days in complete darkness 1) Maruyama, K. and Sugano, S. : Gene, 138, 171 (1994) 2) Tokunaga, K., Taniguchi, H., Yoda, K., Shimizu, M. and Sakiyama, S. : Nucleic Acids Res., 14 (6), 2829 (1986) 3) Leader, D. P., Gall, I., Campbell, P. and Frischauf, A. -M. : DNA, 5, 235 (1986) 4) Peter, B., Man, Y. M., Begg, C. E., Gall, I. and Leader, D. P. : J. Mol. Biol., 203 (3), 665 (1988) 5) in preparation l A. niger (AATase) 010-16731 Alcohol Acetyltransferase ph AATase CoA E. C. : 2. 3. 1. 84 Aspergillus niger 1.5 Umg llllllllllllllllllllllllllllllllllllllllllllllllll 200munit 30,000 1Toshitaka, M. et al. : Bioscience Biotechnology Biochemistry, 57, 2094 (1993) 2) Yoshioka, K. et al. : Agric. Biol. Chem., 46, 2155 (1982)

DCalcein-AM D D DHeLaHL605025,000 D100 Calcein-AM in DMSO PBS( ) Cell Counting Kit-FDMSOPBS () 50 ( ) 10l 30 (ex=485nm,em=535nm)

Q A Q &A l URL : http://www.dojindo.co.jp/ E-mail : info @ dojindo.co.jp 0120-021557 0120-489548 Q A Q A

MTT l 537-33452 539-33451 00-025 00-100 alamar Blue 25ml 100ml 18,500 44,500 51 Cr AM AM

LDH () (3.7mgvial) NAD 5ml10 DL- (50mgml) 55ml (1moll) 55ml 10 () 2 340700nm 260700nm 340700nm 696384 (065,000) (Gain 1255) Excel XFluor

l l sssssssssssssssssssssssssssssssssssss () () --D- ET-301 BL 0.2ml25 500ng (LPS) Wako l

l l l l l l sssssssssssssssssssssssssssssssssssss LED () () SLP 3037 299-28351 297-28651 291-28051 1,900,000 1,800,000 200,000 200,000 291-25751

O CH 3 O COOH H N O N N H O H CH 3 COOH B ( B) DNA 95 (HPLC) DMSO HO H 3 C CH 2 H 3 C H CH 3 CH 3 H H CH 3 H CH 3 COOH HIV 97 (HPLC) DMSO TNFNGF Fas -S-Fas 1 (SDS-PAGE) OH O O NS S N CH 3 CH 2 OH (IC501.1M) Ras 98 (TLC) DMSO, H 3 C CH 3 CH 3 O N H OH all-trans-retinoic Acid (RA) 97 (TLC), DMSO,

O H 3 C S H H F COOH G0G1HT29 HT-29cdks 99 DMSO () WAKO BIO WINDOW FAX: 06-201-5965 E-mail : biowin@wako-chem. co. jp ggggggggggggggggggggggggggggggg Fas Fas Fas l l l l l l 1) Trauth, B. C. et al. : Science, 245, 301 (1989) 2) Friesen, C. : Nature medicine, 2, 574 (1996)

p53in vitro p21 ras in vitro ras ELISAArg12p21, Val12p21, Asp12p21, Asp13p21 ras6080 neu c-erbb2neu WAF1p21in vitro p53waf1p21 (NMP) 417 NMPNMP MTT NMPSCID bcl-2in vitro bcl-2 FasAssay FasFas Fas ELISA p53in vitro

HPLC98.0 HPLC98.0 HPLC95.0 HPLC98.0 HPLC98.0 9-cis-Retinoic Acid all-trans-retinoic Acid 9,13-di-cis-Retinoic Acid Retinol Acetate Standard Solution -Carotene Lycopene (PTPase) CD45VHR G1S CD45IC50=54M VHRIC50=M (HPLC) 95.0 HO HO O O O 1) Hamaguchi, T. et al.: FEBS Lett., 372,54 (1995) 2) Fujii, S. et al.: Neurosci. Lett., 187,133 (1995)

k l SynPhase (TranStems) (TranSort) 96 () TranStems TranSort1050,000 Excel SynPhase Crown 1005,00010,000 1Central unit - low power 4. 9pin to 25pin adapter plug 2Antenna 5. Power supply 3RS232 cable 6. TranSort software (Windows95NT) 538-61871 KT-TS-I-XXX TranSort Package 1 981,000 535-61881 ST-TW-X-NAT TranStems 100 207,000

1020cm HYPER CLEAN () s s s soa s s 306-05735 HYPER CLEAN EE-3310 500ml 4,000 309-05725 HYPER CLEAN EE-3320 500ml 4,000 302-05715 HYPER CLEAN EE-6310 500ml 3,000

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MMP prommp-2 MMP-2MMP-3proMMP-9MMP-9MT- MMP (MMPs) 210 (12) 110 (W)100 (H)1mm (5) (10) 150ml A (10) 100ml B (10) 100ml (10) 25ml 5ml 100ml MMP (prommp-2mmp-2prommp-9) 0.2ml WAKO BIO WINDOW No.10 FEB. 1998 636-01321 YU-68001 1 60,000 133-12631 prommp-2 1ml 42,000 130-12641 prommp-9 1.5ml 42,000 0540-8605 12 106203-3741 0103-0023 513 1033270-8571 1092622-1005 1082285-6381 1052772-0788 1045476-2061 1048641-1271 1029858-2278 1022222-3072 1011271-0285