111-24500035-N-05 21300AMY00324000 20116 2 20099 1 BD BBLCRYSTAL E/NF BD BBLCRYSTAL E/NF 4-A 4-B 4-C 4-D 4-E 4-F 4-G 4-H 4-I 4-J 2-A 2-B 2-C 2-D 2-E 2-F 2-G 2-H 2-I 2-J 1-A 1-B 1-C 1-D 1-E 1-F 1-G 1-H 1-I 1-J p-n-p- p-n-p--- p-n-p-- p-n-p- p-n-p- p-n-p-- p-n-p- p-n-p-- p-n-p-n- L---p- g/ L- 1750.0 3.5 D- 1500.0 3.5 1397.5 3.5 500.0 3.5 L- 1500.0 3.5 D- 1750.0 3.5 D- 900.0 3.5 1250.0 3.5 D- 750.0 3.5 myo- 650.0 3.5 p- 12.5 p---d- 12.5 p---d- 12.5 p---d- 31.0 L-p- 35.0 10.0 p---d- 15.0 p---l- 15.0 p- 15.0 p---d- 10.0 p--n---d- 20.0 ---p- 15.0 71.0 25.0 p--dl- 50.0 150.0 100.0 6.4 350.0 6.4 400.0 6.4 750.0 6.4 75.0 L 750.0 5.0 0.25 L- 250.0 5.0 1
1BD BBLCRYSTAL E/NF Acinetobacter baumannii Acinetobacter lwoffii Aeromonas caviae Aeromonas hydrophila Aeromonas sobria Aeromonas veronii Agrobacterium tumefaciens Bergeyella zoohelcum Brevundimonas diminuta Brevundimonas vesicularis Burkholderia gladioli Burkholderia cepacia Burkholderia pseudomallei Cedecea davisae Cedecea lapagei Cedecea neteri Chromobacterium violaceum Chryseobacterium gleum Chryseobacterium indologenes Chryseomonas luteola Citrobacter amalonaticus Citrobacter freundii Citrobacter koseri Edwardsiella hoshinae Edwardsiella tarda Elizabethkingia meningoseptica Empedobacter brevis Enterobacter aerogenes Enterobacter asburiae Enterobacter cancerogenus Enterobacter cloacae Enterobacter gergoviae Enterobacter sakazakii Escherichia coli Escherichia coli serogroup O111 Escherichia coli serogroup O157 Escherichia coli AD Escherichia fergusonii Escherichia hermanii Escherichia vulneris Ewingella americana Flavimonas oryzihabitans Grimontia hollisae Mannheimia haemolytica Myroides odoratus Hafnia alvei Klebsiella oxytoca Klebsiella pneumoniae ssp. ozaenae Klebsiella pneumoniae ssp. pneumoniae Klebsiella pneumoniae ssp. rhinoscleromatis Kluyvera ascorbata Kluyvera cryocrescens Leclercia adecarboxylata Moellerella wisconsensis Morganella morganii Pantoea agglomerans Pasteurella aerogenes Pasteurella multocida Photabacterium damselasubsp. damsela Plesiomonas shigelloides Proteus mirabilis Proteus penneri Proteus vulgaris Providencia alcalifaciens Providencia rettgeri Providencia rustigianii Providencia stuartii aeruginosa fluorescens putida stutzeri Rahnella aquatilis Raoultella ornithinolytica Salmonella Choleraesuis Salmonella enterica subsp. arizonae Salmonella Paratyphi A Salmonella species Salmonella Typhi Salmonella gallinarum Serratia ficaria Serratia fonticola Serratia liquefaciens Serratia marcescens Serratia odorifera 1 Serratia odorifera 2 Serratia plymuthica Serratia rubidaea Shewanella putrefaciens Shigella dysenteriae Shigella species Shigella sonnei Sphingobacterium multivorum Sphingomonas paucimobilis Stenotrophomonas maltophilia Tatumella ptyseos ibrio alginolyticus ibrio cholerae ibrio fluvialis ibrio metschnikovii ibrio mimicus ibrio parahaemolyticus ibrio vulnificus Yersinia enterocolitica group Yersinia pestis Yersinia pseudotuberculosis Yokenella regensburgei Misc. Gram Negative Bacilli 1 http://www.dsmz.de/index.htm 1 E/NF 1 12 123 Alcaligenes faecalis Methylobacterium species Achromobacter piechaudii Moraxella lacunata Achromobacter xylosoxidans subsp. denitrificans Moraxella osloensis Achromobacter xylosoxidans subsp. xylosoxidans Ochrobactrum anthropi Bordetella bronchiseptica Oligella urethralis Palstonia () pickettii alcaligenes CDC group I C-2 fluorescens 2 Delftia acidovorans mendocina Comamonas testosteroni pseudoalcaligenes Eikenella corrodens putida 2 2 2
2BD BBLCRYSTAL E/NF 4-A ARA 4-B MNS 4-C SUC 4-D MEL 4-E 4-F RHA SOR ph 4-G MNT 4-H ADO 4-I GAL 4-J INO 2-A 2-B 2-C p-n-p- p-n-p--- p-n-p-- PHO BGL NPG p- 2-D PRO p- 2-E p-n-p- BPH 2-F 2-G 2-H 2-I p-n-p- p-n-p-- p-n-p- p-n-p-- BXY AAR PHC GLR p- 2-J p-n-p-n- NAG 1-A L---p- GGL p- 1-B ESC 1-C PHE 1-D URE ph 1-E GLY ph 1-F CIT ph 1-G MLO ph 1-H TTC 1-I 1-J ARG LYS ph 3
3BD BBLCRYSTAL E/NF 4-A ARA / / 4-B MNS / / 4-C SUC / / 4-D MEL / / 4-E RHA / / 4-F SOR / / 4-G MNT / / 4-H ADO / / 4-I GAL / / 4-J INO / / 2-A p-n-p- PHO 2-B p-n-p--- BGL 2-C p-n-p-- NPG 2-D PRO 2-E p-n-p- BPH 2-F p-n-p- BXY 2-G p-n-p-- AAR 2-H p-n-p- PHC 2-I p-n-p-- GLR 2-J p-n-p-n- NAG 1-A L---p- GGL 1-B ESC / / 1-C PHE / 1-D URE / / 1-E GLY / / 1-F CIT / / 1-G MLO / / 1-H TTC / 1-I ARG / / 1-J LYS / / 5 24 1 40 60% BD BBLCRYSTAL Manual of Clinical Microbiology BD BBLCRYSTAL E/NF 20 4
2.20.1mL 1 NaCl8.50g 3-0.8372g BBL CRYSTAL E/NF 20 20 2 1 CO 2 3537 4060%BBLCRYSTAL / BBLCRYSTAL BBLCRYSTAL 5% BBL DMACA BBL BD BBLCRYSTAL E/NF 225 BBLCRYSTAL 10 1 2 225 BBLCRYSTAL 10 1 2 225 BBLCRYSTAL E/NF BD BBLCRYSTAL E/NF 225 1525 BBLCRYSTAL E/NF 24 1 1 1 1 2 5 23mm 1 45 5
3 4 1015 5 6 2 2 7 3 3 8 1 4 4 9 2 5 5 1 3537 1824 1 10 2 5 CO 2 4060% 2 E/NF 3537 1820 6 6 6
3 BBLCRYSTAL E/NF 7 7 BD BBLCRYSTAL 421 0 10 A B C D E F G H I J 4 2 1 5 4 6 0 2 4 4 3 7 1 BBLCRYSTAL 1BBLCRYSTAL E/NF Klebsiella pneumoniae ATCC 33495 2 3 5 1525 3 BBLCRYSTAL E/NF 435 53537 1820 6BBLCRYSTAL BBLCRYSTAL E/NF 7 4 5 7
8 4BD BBLCRYSTAL E/NF 4-A 4-B 4-C 4-D 4-E 4-F 4-G 4-H 4-I 4-J 2-A 2-B 2-C 2-D 2-E 2-F 2-G 2-H 2-I 2-J 1-A 1-B 1-C 1-D 1-E 1-F 1-G 1-H 1-I 1-J p-n-p- p-n-p--- p-n-p-- p-n-p- p-n-p- p-n-p-- p-n-p- p-n-p-- p-n-p-n- L---p- ARA MNS SUC MEL RHA SOR MNT ADO GAL INO PHO BGL NPG PRO BPH BXY AAR PHC GLR NAG GGL ESC PHE URE GLY CIT MLO TTC ARG LYS Klebsiella pneumoniae ATCC 33495
9 5BD BBLCRYSTAL E/NF 4-A 4-B 4-C 4-D 4-E 4-F 4-G 4-H 4-I 4-J 2-A 2-B 2-C 2-D 2-E 2-F 2-G 2-H 2-I 2-J 1-A 1-B 1-C 1-D 1-E 1-F 1-G 1-H 1-I 1-J ARA MNS SUC MEL RHA SOR MNT ADO GAL INO PHO BGL NPG PRO BPH BXY AAR PHC GLR NAG GGL ESC PHE URE GLY CIT MLO TTC ARG LYS Escherichia coli ATCC 25922 Acinetobacter lwoffii ATCC 17925 Proteus vulgaris ATCC 8427 Enterobacter cloacae ATCC 35030 aeruginosa ATCC 35032
3 E/NF 30 96.3100% BD BBLCRYSTAL E/NF 99.6% 1 BD BBLCRYSTAL E/NF 169 45 N=169 BD BBLCRYSTAL E/NF 16396.4% 16798.8% 21.2% 2 BD BBLCRYSTAL E/NF 3 299 96.7% 289 23 16 291 96.9%282 2 8 1 HI, HB, HC 2 BD BBLCRYSTAL E/NF E/NF 1 BD BBLCRYSTAL E/NF 3 AD 1 10
5% 3 225 12 20 11
OFBM CDCc-2 OFBM Alcaligenes faecalis Ochrobactrum anthropi Bordetella bronchiseptica Alcaligenes xylosoxidans subsp. xylosoxidans Alcaligenes xylosoxidans subsp. denitrificans Alcaligenes piechaudii OFBM OFBM OFBM 42 mendocina fluorescens Burkholderia () pickettii putida pseudoalcaligenes 1 1 Methylobacterium subsp. OFBM1% Comamonas acidovorans pseudoalcaligenes alcaligenes Methylobacterium subsp. Methylobacterium subsp. Comamonas testosteroni Eikenella corrodens Moraxella lacunata Oligella urethralis Moraxella osloensis 1.Gilardi, G, L., Identification of Glucose-Nonfermenting Gram-Negative Rods, 1/90 2.Manual of Clinical Microbiology, 5th Edition, American Society for Microbiology, Washington, D.C., 1991 BDBD 960-21521 TEL.0120-8555-90 FAX.024-593-5761 1 TEL.0120-8555-90 107-0052 4-15-1 www.bd.com/jp/ 12