RNA produced by RNA Ambion
RNA 2014 10 produced by RNA Ambion Total RNA P6 PureLink RNA Mini Kit 10 preps 12183020 9,700 PureLink RNA Mini Kit 50 preps 12183018A 30,000 PureLink RNA Mini Kit 250 preps 12183025 126,000 PureLink RNA Micro Kit 50 preps 12183016 42,000 TRIzol Reagent 100 ml 15596026 25,700 TRIzol Reagent 200 ml 15596018 45,500 TRIzol LS Reagent 100 ml 10296010 25,500 TRIzol LS Reagent 200 ml 10296028 45,000 TRIzol Max Bacterial RNA Isolation kit TRIzol Reagent 100mL + Enhancement Reagent 20mL 16096020 33,900 TRIzol Max Bacterial RNA Isolation kit TRIzol Reagent 200mL + Enhancement Reagent 40mL 16096040 50,700 TRIzol Plus RNA Purification System 50 preps 12183555 52,500 MagMAX -96 Total RNA Isolation Kit 96 rxns AM1830 62,500 MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 77,900 MagMAX -96 Viral RNA Isolation Kit 50 rxns AM1939 42,700 MagMAX -96 Viral RNA Isolation Kit 96 rxns AM1836 64,800 MagMAX Total Nucleic Acid Isolation Kit 100 rxns AM1840 115,800 MagMAX -96 Blood RNA Isolation Kit 96 rxns AM1837 69,100 mrna P12 Poly A Purist Kit 6 rxns AM1916 MicroPoly A Purist Kit 20 rxns AM1919 FastTrack 2.0 mrna Isolation Kit 6 rxns K159302 FastTrack 2.0 mrna Isolation Kit 18 rxns K159303 Poly A Purist MAG Kit 1 kit AM1922 94,400 Dynabeads mrna Purification Kit* 2 ml 61006 Dynabeads mrna DIRECT Kit* 5 ml 61011 Dynabeads mrna DIRECT Kit* 10 ml 61012 Dynabeads mrna DIRECT Micro Kit* 2 ml 61021 FastTrack MAG 96 mrna Isolation Kit 2 96 preps K158096 153,300 FastTrack MAG Maxi mrna Isolation Kit 6 preps K158002 102,900 FastTrack MAG Micro mrna Isolation Kit 12 preps K158001 45,200 mrna Catcher PLUS Kit 1 96 preps K157002 42,000 mrna Catcher PLUS Kit 10 96 preps K157003 352,800 MICROBExpress Bacterial mrna Enrichment Kit 20 rxns AM1905 72,300 * P14 TaqMan Gene Expression Cells-to-CT Kit 40rxns 100PCRs 4399002 77,700 TaqMan Gene Expression Cells-to-CT Kit 100rxns 500PCRs AM1728 153,300 TaqMan Gene Expression Cells-to-CT Kit 400rxns 2,000PCRs AM1729 588,000 TaqMan PreAmp Cells-to-CT Kit 40rxns 500PCRs 4387299 289,800 TaqMan Fast Cells-to-CT Kit 100rxns 500PCRs 4399003 153,300 TaqMan MicroRNA Cells-to-CT Kit 100rxns 500PCRs 4391848 199,500 Power SYBR Green Cells-to-CT Kit 40rxns 100PCRs 4402953 72,500 Power SYBR Green Cells-to-CT Kit 100rxns 500PCRs 4402954 128,100 Power SYBR Green Cells-to-CT Kit 400rxns 2,000PCRs 4402955 488,300 Fast SYBR Green Cells-to-CT Kit 100rxns 500PCRs 4402956 128,100 Fast SYBR Green Cells-to-CT Kit 400rxns 2,000PCRs 4402957 493,500 Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr PAXgene or Tempus Blood RNA Tubes 50rxns 4449079 47,300 Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr PAXgene Blood RNA Tubes Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr Tempus Blood RNA Tubes 200rxns 4449082 164,900 200rxns 4449080 162,800 Ambion Single Cell-to-CT Kit 50rxns 4458237 178,500 Ambion Single Cell-to-CT Kit 400rxns 4458236 1,029,000 mirna P18 mirvana mirna Isolation Kit 40 rxns AM1560 52,800 mirvana PARIS Kit 40 rxns AM1556 60,700 MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 77,900 TaqMan MicroRNA Cells-to-CT Kit 100 rxns 500 PCRs 4391848 199,500 RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues 40 rxns AM1975 58,000
RNase P20 Conical Tubes 15 ml racked 500 tubes AM12500 44,100 Conical Tubes 50 ml racked 200 tubes AM12501 22,300 Nuclease-Free Water not DEPC-treated1 500 ml 500 ml AM9930 13,000 DEPC-Treated Water 1 100 ml 100 ml AM9915G 5,500 RNase-free Microfuge Tubes 1000 0.5 ml AM12300 16,500 RNase-free Microfuge Tubes 500 1.5 ml AM12400 8,900 RNase-free Microfuge Tubes 500 2 ml AM12425 11,000 Non-stick RNase-free Microfuge Tubes 500 0.5 ml AM12350 11,000 Non-stick RNase-free Microfuge Tubes 250 1.5 ml AM12450 8,000 Non-stick RNase-free Microfuge Tubes 250 2 ml AM12475 11,000 Elution Tubes 100 2 ml AM12480 15,500 RNase-free Tips, 200 L Size 10 8 12 / AM12650 19,800 RNase-free Tips, 1000 L Size 10 100 / AM12660 20,100 Barrier Filter Tips, 1 ml Size 10 100 / AM12665 28,700 Barrier Filter Tips, 200 L Size 10 8 12 / AM12655 28,700 Barrier Filter Tips, 100 L Size 10 8 12 / AM12648 28,700 Barrier Filter Tips, 20 L Size 10 8 12 / AM12645 28,700 Barrier Filter Tips, 10 L Size-Pipetman 10 8 12 / AM12640 29,700 Barrier Filter Tips, 10 L Size-Eppendorf 10 8 12 / AM12635 28,700 Thin-walled, Dome Cap, RNase-free PCR Tubes 1000 0.5 ml AM12250 22,100 Thin-walled, Frosted Lid, RNase-free PCR Tubes 1000 0.5 ml AM12275 19,800 Thin-walled, Frosted Lid, RNase-free PCR Tubes 1000 0.2 ml AM12225 28,700 RNaseZap Solution 1 bottle 250 ml AM9780 11,300 RNaseZap Solution 6 bottles 6 250 ml AM9782 51,100 RNaseZap Solution 1 box 4 L AM9784 108,200 RNaseZap Wipes 100 AM9786 11,300 RNaseZap Wipes Refill 3 100 AM9788 30,600 RNAlater Solution 100 ml AM7020 16,800 RNAlater Solution 500 ml AM7021 54,600 RNAlater Solution 50 1.5mL AM7022 40,400 RNAlater Solution 20 5mL AM7023 40,400 RNAlater Solution 250 ml AM7024 31,500 RNAlater -ICE Solution 25 ml AM7030 17,500 RNAlater -ICE Solution 10 25mL 4427575 66,600 THE RNA Strange Solution 10 tubes AM7000 7,800 THE RNA Strange Solution 50 ml AM7001 7,800 RNA P22 Qubit 2.0 Fluorometer Q32866 183,800 Qubit 2.0 Quantitation Starter Kit 1 Kit Q32871 197,700 Qubit 2.0 Quantitation Lab Starter Kit 1 Kit Q32872 813,800 Qubit dsdna BR Assay Kit 100 assays Q32850 10,500 Qubit dsdna BR Assay Kit 500 assays Q32853 35,000 Qubit dsdna HS Assay Kit 100 assays Q32851 10,500 Qubit dsdna HS Assay Kit 500 assays Q32854 35,000 Qubit ssdna Assay Kit 100 assays Q10212 10,500 Qubit RNA Assay Kit 100 assays Q32852 10,500 Qubit RNA Assay Kit 500 assays Q32855 35,000 Qubit RNA BR Assay Kit 100 assays Q10210 10,500 Qubit RNA BR Assay Kit 500 assays Q10211 35,000 Qubit Protein Assay Kit 100 assays Q33211 10,500 Qubit Protein Assay Kit 500 assays Q33212 35,000 Qubit Assay Tubes 500 tubes Q32856 6,500 2014 10 www.lifetechnologies.com/tc Not for use in diagnostic procedures. 2014 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Printed in Japan. AMB127-C1410IH 108-0023 4-2-8 0120-477-392 TEL 03-6832-6980 FAX 03-6832-9584 TEL 03-6832-9300 FAX 03-6832-9580 www.lifetechnologies.com jptech@lifetech.com facebook.com/lifetechnologiesjapan @LifetechJPN
RNA Ambion Confidence comes naturally Ambion 20RNA RNA RNA 2
4 6 Total RNA 12 mrna 14 18 mirna 20 RNase 22 RNA 3
RNA Ambion RNA RNA RT-PCR qpcr RNA RNA PureLink RNA Mini Kit PureLink Pro 96 Total RNA Purification Kit MagMAX -96 Total RNA Isolation Kit 24 High Throughput Web : Total RNA purification P6 www.lifetechnologies.com/totalrna mirna solutions P18 www.lifetechnologies.com/mirvana Cells-to-CT kits P14 www.lifetechnologies.com/cellstoct NEW Single Cell-to-CT kits P17 www.lifetechnologies.com/sc2ct RNA purification products selection guide www.lifetechnologies.com/rnaselection Ambion Technical Resources www.lifetechnologies.com/rnaexperts F www.lifetechnologies.com/rnapreps MagMAX FFPE Total Nucleic Acid Isolation Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE 1 50 Low-Middle Throughput 17 High Throughput 16 Low Throughput 4
TRIzol Plus RNA Purification System 1 50 Low-Middle Throughput PureLink Pro 96 Total RNA Purification Kit MagMAX -96 for Microarrays Total RNA Isolation Kit 24 High Throughput TRIzol Plus RNA Purification System MagMAX Total Nucleic Acid Isolation Kit Cells-to-CT kits 10 10 5 cells Single Cell-to-CT Kit Single cell PureLink RNA Mini Kit 1 50 Low Throughput PureLink Pro 96 Total RNA Purification Kit MagMAX -96 Total RNA Isolation Kit 24 High Throughput 24 or PCR PCR PCR FFPE 500 µl LCM 1 50 Low-Middle Throughput 24 High Throughput RNA Single Cell-to-CT Kit PureLink RNA Micro Kit 3 ml TRIzol LS Reagent MagMAX -96 Blood RNA Isolation Kit mirvana mirna Isolation Kit or RNA PureLink Total RNA Blood Kit Tempus tubes Stabilized Blood-to-CT kits LeukoLOCK Kit MagMAX for Stabilized Blood Tubes kits Tempus kits 5
Total RNA PureLink RNA Mini Kit Total RNA PureLink RNA Mini Kit Total RNA 1PureLink RNA Mini Kit 1 RNA RNA RNase 20RNA 1,000 µg RNA RNA PureLink 20 RNARNA DNA RT-PCR qpcr cdna Mini KitMidi Kit Total RNA23 PureLink RNA Mini Kit 1 PureLink RNA Mini Kit 1 4 RNA SPIN 3 10 8 200 mg 250 mg 0.2 ml 5 x 10 8 10 9 1,000 2 5 RNA RNA (µg) 900 800 700 600 500 400 PureLink RNA Mini Kit Qiagen Q RNA RNeasy Midi Kit RNA Midi Kit Q RNA Mini Kit 300 200 SPIN 3 RNA 100 0 25 100 200 Tissue (mg) 2 PureLink RNA Mini Kit: 200 mg PureLink RNA Mini Kit Q Kit Mini Midi25 mg 100 mg 200 mg RNA Total RNA PureLink RNA Mini Kit Q Midi Kit PureLink RNA Mini Kit www.lfetechnologies.com/rnamini 6
PureLink RNA Micro Kit RNA PureLink RNA Micro KitLCM FACS RNA TRIzol Reagent 10 mg 15100 µg RNA Total RNA 85 Total RNA Yield (ug) 20 18 16 14 12 10 8 6 4 2 0 Liver Spleen Kidney Brain Heart Testes Rat Tissue Sample 1 Sample 2 Sample 3 3 PureLink RNA Micro Kit 5mg RNA RNA 10 l RNA 260nm 2 PureLink RNA Micro Kit Cells HeLa 5 10 5 7.5 µg HEK293 5 10 5 10 µg Rat liver 5 mg 17 µg Rat brain 5 mg 2 µg Rat spleen 5 mg 13.1 µg Rat heart 5 mg 1.8 µg Rat testes 5 mg 7.1 g 3 PureLink PureLink RNA Mini Kit Q Mini Kit Q Midi Kit PureLink RNA Micro Kit Q Micro Kit 200 mg 5 x 10 7 0.5 30 mg 10 7 20 250 mg 5 x 10 6 1 x 10 8 5 mg 5 x 10 6 5 mg 5 x 10 5 1,000 µg 100 µg 1,000 µg 100 µg 45 µg 20 20 60 15 30 40 30 100 µl 30 100 µl 300 500 µl 10 12 µl 10 14 µl Ordering information PureLink RNA Mini Kit 10 preps 12183-020 50 preps 12183-018A 250 preps 12183-025 PureLink RNA Micro Kit 50 preps 12183-016 7
Total RNA TRIzol Reagents TRIzol Reagents DNATotal RNA 4 Chomczynski Sacchi RNA TRIzol Reagents RNA RNA TRIzol ReagentsRNA 50 100 mg 5 x10 6 1 g >10 7 TRIzol Reagent 1 TRIzol Reagent Total RNADNA 4 TRIzol Reagents TRIzol 100 mg10 7 1 ml TRIzol LS 5 x 10 6 0.75 ml TRIzol Max Bacterial RNA Isolation Kit 10 8 Max Bacterial Enhancement Reagent 0.2 ml + TRIzol Reagent 1 ml RNA RNase RNase RNA RNA RNase Ordering information TRIzol Reagent 100 ml 15596-026 200 ml 15596-018 TRIzol LS Reagent 100 ml 10296-010 200 ml 10296-028 TRIzol Max Bacterial RNA Isolation kit TRIzol Reagent 100mL + Enhancement Reagent 20mL 16096-020 TRIzol Reagent 200mL + Enhancement Reagent 40mL 16096-040 www.lifetechnologies.com/trizol 8
TRIzol Plus RNA Purification System RNA TRIzol Plus RNA Purification SystemTRIzol Reagent PureLink RNA Mini Kit 4 TRIzol Plus RNA Purification System 1 RNA 55 Total RNAqPCR 1,000 µg Total RNA RNA Total RNAg 1,000 100 10 1 TRIzol Plus RNA Purification System Q Mini Kit 1 10 100 1,000 Starting material mg 5 TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Q Total RNA TRIzol Plus System 1,000 µg RNA Q 1/10 4 TRIzol Reagent RNA RNA TRIzol RNA SPIN SPIN RNA PureLink RNA RNA RNase RNA RNase RNA 5 TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Q Reagent+ Mini Kit TRIzol Reagent 100 ml + 50 Q Reagent 200 ml + 50 200 mg10 8 30 mg10 7 1,000 µg 100 µg 45 45 30 µl 30 100 µl Ordering information TRIzol Plus RNA Purification System 50 preps 12183-555 9
Total RNA MagMAX purification systems RNA MagMAX TM MagMAX TM 96 6 7MagMAX TM 10 5 RNA RT- PCR100 RNA RNA 1 C t 40 35 30 Slope = -3.7 ±0.1 R 2 = 0.996 20 50 µlrna ΔRn 25 20 0.1 10 3 10 4 10 5 10 6 Cell # 0.01 0 10 20 30 40 1. Cycle 2. RNA 7 Total RNA MagMAX TM -96 Total RNA Isolation Kit 100 2 x 10 6 K562 RNA 8 n=8 4 qrt-pcr 10 µl RNA Polymerase II mrna 8 n=8 Ct CV 3 3. 4. RNA 6 The MagMAX Isolation Kit 10
6 MagMAX TM Kits: MagMAX -96 Total RNA Isolation Kit MagMAX -96 for Microarrays Total RNA Isolation Kit 2 x 10 6 5 x 10 7 5 mg 10 mg Plant RNA Isolation Aid 100 mg gdnaturbo DNase TM 96 96 mirna MagMAX -96 Viral RNA Isolation Kit MagMAX Total Nucleic Acid Isolation Kit MagMAX -96 Blood RNA Isolation Kit RNA DNA RNA DNA 50 400 µl 175 µl 300 mg 175 µl 300 mg 50 µl DNA 50 µl gdna 50 96 96 Ordering information MagMAX -96 Total RNA Isolation Kit 96 rxns AM1830 MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 MagMAX -96 Viral RNA Isolation Kit 50 rxns AM1939 96 rxns AM1836 5 x 96 rxns AMB18365 MagMAX Total Nucleic Acid Isolation Kit 100 rxns AM1840 MagMAX -96 Blood RNA Isolation Kit 96 rxns AM1837 www.lifetechnologies.com/magmax 11
mrna Total RNA Total RNAmRNA mrna Total RNA mrna 2 mrna Total RNA mrna dt/dt / 7 mrna dt/ Poly A Purist TM KitMicroPoly A Purist TM Kit rrna mrna 1 2mRNA FastTrack 2.0 mrna Isolation Kit mrnatotal RNA mrna FastTrack 2.0 mrna Isolation Kit mrna Total RNA mrna dt / Poly A Purist TM MAG Kit Poly A Purist TM Kit 45 8 1 mg Total RNA 80 30 µg Total RNA 7 mrna Total RNA mrna Poly(A)Purist MicroPoly(A)Purist Poly (A) Purist MAG Dynabeads mrna Purification MICROBExpress Bacterial mrna mrna FastTrack 2.0 mrna Isolation Dynabeads mrna DIRECT FastTrack MAG mrna Catcher PLUS dt Poly A RNA 96 mrna Total RNA 95rRNA 12
Dynabeads mrna Purification Kit for mrna Purification from Total RNA Preps Total RNAmRNA 15 5 µlmrna cdna Dynabeads mrna DIRECT TM 15 5 µlmrna cdna FastTrack MAG Kit mrna Total RNA dt FastTrack MAG Micro mrna Isolation Kit 50 mg Total RNA 10 6 50 mg FastTrack MAG Maxi mrna Isolation Kit 2,000 µg Total RNA 5 x 10 7 500 mg mrna Catcher TM PLUS Kit 100 ng 100 µg Total RNA 10010 6 4 mg 40 µl 80 ng MICROBExpress TM Bacterial mrna Enrichment mrnamrna mrna Total RNA16S 23S rrna 95% mrna Ordering information Poly(A)Purist Kit 6rxns AM1916 MicroPoly(A)Purist Kit 20rxns AM1919 FastTrack 2.0 mrna Isolation Kit 6rxns K1593-02 18rxns K1593-03 Poly(A)Purist MAG Kit 1 kit AM1922 Dynabeads mrna Purification Kit 2mL 610-06 Dynabeads mrna DIRECT Kit 5mL 610-11 10mL 610-12 Dynabeads mrna DIRECT Micro Kit 2mL 610-21 FastTrack MAG 96 mrna Isolation Kit 2 96 preps K1580-96 FastTrack MAG Maxi mrna Isolation Kit 6 preps K1580-02 FastTrack MAG Micro mrna Isolation Kit 12 preps K1580-01 mrna Catcher PLUS Kit 1 x 96 preps K1570-02 10 x 96 preps K1570-03 MICROBExpress Bacterial mrna Enrichment Kit 20rxns AM1905 13
Cells-to-CT kits RNA RT-PCR Cells-to-CT TM Kits RT-PCRRNA RNA 96 384 11 Cells-to-CT TM Kits TaqMan SYBR GreenPCR 8 8 Cell-to-CT TM Kits TaqMan Gene Expression Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit Application KitPCR master mix TaqMan Gene Expression Master Mix TaqMan PreAmp Reagents TaqMan Gene Expression Master Mix TaqMan Fast Universal PCR Master Mix 7 60 PCR 90 7 60 PCR 150 7 60 PCR 35 10 100,000 10 100,000 10 100,000 sirna Pre-PCR Pre-PCR100 64 Fast PCR PCR 40 Fast Ordering information TaqMan Gene Expression Cells-to-CT Kit 40rxns 100PCRs 4399002 100rxns 500PCRs 400rxns 2,000PCRs AM1728 AM1729 TaqMan PreAmp Cells-to-CT Kit 40rxns 500PCRs 4387299 TaqMan Fast Cells-to-CT Kit 100rxns 500PCRs 4399003 TaqMan MicroRNA Cells-to-CT Kit 100rxns 500PCRs 4391848 Power SYBR Green Cells-to-CT Kit 40rxns 100PCRs 4402953 100rxns 500PCRs 4402954 400rxns 2,000PCRs 4402955 14
7 10 min total 5x 5' 5' 3' 3' 3' mrna 5' RT primer 3' 5' cdna 1. 2. RT 3. PCR 1PBS 2 5 3 2 1 RT 2 RT65 PCR cdna 2 PCR PCR 8 Cells-to-CT TM Kit TaqMan MicroRNA Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit Stabilized Blood-to-CT Single Cell-to-CT mirna TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix TaqMan Gene Expression Master Mix 10 60 PCR 90 7 60 PCR 90 7 60 PCR 35 60 7 10 100,000 10 100,000 10 100,000 500 µl 1 10 TaqMan Micro RNA Assays Fast PCR PCR 40 Fast Total RNA mirna PAXgene Tempus TM Blood RNA Tubes RT-PCR Ordering information Fast SYBR Green Cells-to-CT Kit 100rxns 500PCRs 4402956 400rxns 2,000PCRs 4402957 Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (PAXgene or Tempus Blood RNA Tubes) Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (PAXgene Blood RNA Tubes) Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qpcr (Tempus Blood RNA Tubes) 50rxns 4449079 200rxns 4449082 200rxns 4449080 Ambion Single Cell-to-CT Kit 50rxns 4458237 400rxns 4458236 Cells-to-CT family www.lifetechnologies.com/cellstoct 15
Cells-to-CT kits RNA Cells-to-CT Cells-to-CT 8 RT-PCR RNA 9 RNA 10 Panel A. 18S rrna Cells-to-CT Lysate Purified RNA Cells-to-CT Lysate Purified RNA Panel B. GAPDH Cells-to-CT Lysate Purified RNA Panel C. ACTB 9 Cells-to-CT Kit RT-PCR HeLa RNA Cell-to-CT Kit 128 TaqMan Gene Expression Assays Cell-to-CT Kit CT Cell-to-CT Kit RT- 10 Cells-to-CT Kit RNA 10 100,000 HeLa RNA Taq- Man Gene Expression Master Mix TaqMan Human Endogenous Control Arrays Panel A~C Endogenous Control Arrays 16 3 Ambion Cells-to-CT TM PCR YouTubeAmbion Cells 33 31 29 27 RNA -3.69 R 2 = 1.00 TaqMan Gene Expression Cells-to-CT TM Kit -3.36 R 2 = 0.99 A -3.89 R 2 = 1.00 Q -3.96 R 2 = 0.99 C t 25 11 TaqMan Gene Expression Cells-to-CT TM Kit Hela 100 10,000 RNA TaqMan Gene Expression Cells-to-CT TM Kit Q A RNA PCR PPIA TaqMan Gene Expression Assay 3 Cells-to-CT TM Kit Kit RNA 10 100 RNA 23 21 19 17 15 10 E2 10 E3 10 E4 10 E5 16
Single Cell-to-CT Kit RT-PCR 5 13 14 Ambion Single Cell-to-CT Kit1-10 12 1. 2. 3. RT 4. 5. PCR 1. 10 Single Cell Solution 1 10 2. 5 1. 1 Stop Solution 2. 2 1. 4-5 RT Mix 2. 25 10 42 60 85 5 1. 11 PreAmp Mix 2. 95 10 95 15 60 4 PCR 1. Preamplified 1 20 PCR 12 Ambion Single Cell-to-CT Kit Single Cell-to-CT Kit 13 Single Cell-to-CT Kit 100 6.8 CT 14 10 1. Single Cell Lysis Buffer 25 5 2. PBS / 5 3. PBS RT 95 10 cdna 14 Pre-Amplification B2M GAPDH HPRT1 TGFB1 5 CT 17
mirna mirna mirna Small RNA RNA mirnanon-coding RNA22 mrna mirna Small RNA mirna mirna RT-PCR 9 RNA 200 nt Small RNA mirvana mirna Isolation Kit RNA Total RNA RNA 200 nt mirna sirna shrna snrna 9miRNA mirvana mirna Isolation Kit mirvana PARIS Kit RNA mirna Total RNA DNA ü ü ü 10 3 10 7 0.5 250 mg 100 10 7 100 mg mirna RNA 30 mrna mirnasirna RNA Small RNA Ordering information mirvana mirna Isolation Kit 40 rxns AM1560 mirvana PARIS Kit 40 rxns AM1556 18
TaqMan MicroRNA Cells-to-CT Kit RNA mirna 10 100,000 10 RT-PCRTaqMan MicroRNA Assays RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues FFPE RNA RNA overnight ProteinaseK RT-PCR 50% MagMAX -96 for Microarrays Total RNA Isolation Kit TaqMan MicroRNA Cells-to-CT Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues ü ü ü ü Cells-to-CT TM Kit DNase 5 x 10 6 100 mg 10 100,000 420 µm RNA RNA RT-PCR TaqMan MicroRNA Assays FFPE RNA RNAovernight Proteinasek RT-PCR 50% RT-PCR PCR Ordering information MagMAX -96 for Microarrays Total RNA Isolation Kit 96 rxns AM1839 TaqMan MicroRNA Cells-to-CT Kit 100 rxns (500 PCRs) 4391848 400 rxns (2,000 PCRs) 4391996 RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues 40 rxns AM1975 19
RNase Ambion RNA Essentials Products RNase RNase RNase RNA 1 RNase 2 3RNase RNase DNase nuclease-free nuclease RI RNA3714 16 RNA RNaseZap Solution RNaseZap Wipes ElectroZap TM Solution RNase AWAY Reagent RNase RNaseZap Solution RNase RNase RNaseZap Wipes l / Hind III Markers A B RNaseZap Ordering information Conical Tubes (15 ml) (racked) 500 tubes AM12500 Conical Tubes (50 ml) (racked) 200 tubes AM12501 Nuclease-Free Water (not DEPC-treated) 500 ml AM9930 (1 x 500 ml) DEPC-Treated Water (1 x 100 ml) 100 ml AM9915G RNase-free Tip Tube RNase-free Microfuge Tubes 1,000 x 0.5 ml AM12300 RNase-free Microfuge Tubes 500 x 1.5 ml AM12400 RNase-free Microfuge Tubes 500 x 2.0 ml AM12425 Non-stick RNase-free Microfuge Tubes 500 x 0.5 ml AM12350 Non-stick RNase-free Microfuge Tubes Non-stick RNase-free Microfuge Tubes Elution Tubes RNase-free Tips, 200 μl Size RNase-free Tips, 1000 μl Size Barrier (Filter) Tips, 1 ml Size Barrier (Filter) Tips, 200 μl Size Barrier (Filter) Tips, 100 μl Size Barrier (Filter) Tips, 20 μl Size Barrier (Filter) Tips, 10 μl Size -Pipetman Barrier (Filter) Tips, 10 μl Size -Eppendorf Thin-walled, Dome Cap, RNase-free PCR Tubes Thin-walled, Frosted Lid, RNase-free PCR Tubes Thin-walled, Frosted Lid, RNase-free PCR Tubes RNaseZap Solution 250 x 1.5 ml AM12450 250 x 2.0 ml AM12475 100 x 2.0 ml AM12480 10 AM12650 (8 x 12 / ) 10 AM12660 (100 / ) 10 AM12665 (100 / ) 10 AM12655 (8 x 12 / ) 10 AM12648 (8 x 12 / ) 10 AM12645 (8 x 12 / ) 10 AM12640 (8 x 12 / ) 10 AM12635 (8 x 12 / ) 1,000 x 0.5 ml AM12250 1,000 x 0.5 ml AM12275 1,000 x 0.2 ml AM12225 RNaseZap Solution (1 bottle) 250 ml AM9780 RNaseZap Solution (6 bottles) 6 x 250 ml AM9782 28S rrna 18S rrna RNase RNaseZap Solution (1 box) 4 L AM9784 RNaseZap Wipe RNaseZap Wipes 100 AM9786 RNaseZap Wipes Refill 3 x 100 AM9788 20
RNase RNA RNA RNA RNA RNA RNAlater RNA : RNaseRNA 371251 41-20RNA RNAlater Solution : cdna RNA RNA RNA RNAlater -ICE RNA RNA RNA RNA Storange Solution RNA 0.1mµ EDTA TE Buffer RNAlater Solution RNAlater Solution XenopusDrosophila RNAlater Solution RNaseRNA 37 C 4 C RNA RNA p53 GAPDH p53 RNAlater Solution RNAlater Solution 100 ml AM7020 RNAlater Solution 500 ml AM7021 RNAlater Solution 50 x 1.5 ml AM7022 RNAlater Solution 20 x 5 ml AM7023 RNAlater Solution 250 ml AM7024 15 RNAlater RNA GAPDH 5 RNAlater 3725 4p53 GAPDH RNAlater RNA RNase RNAlater -ICE Solution RNAlater -ICE Solution 25 ml AM7030 RNAlater -ICE Solution 10 25 ml 4427575 RNA Strage Solution THE RNA Strange Solution 10 tubes AM7000 THE RNA Strange Solution 50 ml AM7001 21
RNA Qubit 2.0 Fluorometer Qubit 2.0 Fluorometer DNA RNA RT-PCR(qPCR) Qubit 2.0 FluorometerMolecular Probes Qubit DNA RNA 17 Qubit 2.0 Fluorometer 0.01 10ng/ L 10 replicates of lambda DNA Qubit DNA HS Assay kit Qubit 2.0 Fluorometer Spectrophotometer Qubit 17DNA RNA Qubit 10 Qubit 10 pg/µl DNA 12.5 µg/ml 16 The Qubit Fluorometric Quantitation System 22
2-3 2 Protein Assay 15 Qubit 2.0 18 Qubit Qubit 2.0Fluorometer 10 Qubit Qubit dsdna HS Assay 0.2 100 ng 10 pg 100 ng/µl Qubit dsdna BR Assay 2 1,000 ng 100 pg 1 µg/µl Qubit ssdna Assay 1 200 ng 50 pg 200 ng/µl Qubit RNA Assay 5 100 ng 250 pg 100 ng/µl Qubit RNA BR Assay 20 1,000 ng 1 ng 1 µg/µl Qubit Protein Assay* 0.25 5 µg 12.5 µg 5 mg/ml Qubit Protein Assay 0.01% SDS2 µl Ordering information Qubit 2.0 Fluorometer Q32866 Qubit 2.0 Quantitation Starter Kit 1 Kit Q32871 Qubit 2.0 Quantitation Lab Starter Kit 1 Kit Q32872 Qubit dsdna BR Assay Kit 100 assays Q32850 500 assays Q32853 Qubit dsdna HS Assay Kit 100 assays Q32851 500 assays Q32854 Qubit ssdna Assay Kit 100 assays Q10212 Qubit RNA Assay Kit 100 assays Q32852 500 assays Q32855 Qubit RNA BR Assay Kit 100 assays Q10210 500 assays Q10211 Qubit Protein Assay Kit 100 assays Q33211 500 assays Q33212 Qubit Assay Tubes 500 tubes Q32856 Qubit www.lifetechnologies.com/qubit 23
Facebook! facebook.com/lifetechnologiesjapan 108-0023 4-2-8 TEL.03-6832-9300 FAX. 03-6832-9580 564-0052 10-28 TEL.06-6339-8165 FAX. 06-6339-8138 0120-650591 0120-477-392 FAX 0120-477-120 TEL 03-6832-6980 FAX 03-6832-9584 TEL 03-6832-6980 FAX 03-6832-9585 TEL 03-5753-3006 FAX 03-5753-3007 TEL 03-6832-9300 FAX 03-6832-9580 The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc. TRIzol is a registered trademark of Molecular Research Center, Inc. 2012 4 2012, Life Technologies Japan Ltd. All rights reserved. Printed in Japan. AMB126-A1209OB www.lifetechnologies.com/tc www.lifetechnologies.com