StepOnePlus / StepOne PCR
PCR Applied Biosystems StepOnePlus StepOne PCR 1
Features At A Glance StepOne StepOnePlus / 48 96 FAM/SYBR Green dyes VIC/JOE dyes ROX dyes NED/TAMRA dyes 1. VeriFlex Block StepOnePlus StepOne PCR 48 StepOne 96 StepOnePlus 1 PCR 1 PCR PCR 2 99.7 10 log 20 μl 10 RNase P StepOne StepOne 96 StepOne StepOnePlus PCR StepOne StepOnePlus 3 48 StepOne 4 96 StepOnePlus PCR LED FAM SYBR Green VIC JOE ROX SNP / StepOnePlus TAMRA 40 Fast PCR LCD USB E StepOnePlus VeriFlex PCR 6 StepOne StepOnePlus 2
StepOnePlus StepOne USB LAN Local Area Network E E StepOnePlus StepOne 5 LAN 4 1. 2. 3. 4. LAN 5. 2. 3. LAN 3 4. StepOne 5
StepOnePlus StepOne PCR 5 4 CT PCR SNP E & PowerPoint Excel jpeg 5. StepOne Multiple Plots 1 4 Multiple Plots SNP 6. StepOne PCR StepOne LAN 4
StepOnePlusStepOne FAMVIC Applied Biosystems TaqMan MGB VIC TAMRA Applied Biosystems SYBR Green I StepOnePlus TAMRATaqMan MGB SYBR Green I 1 StepOnePlus StepOne - - - CT CT StepOnePlus StepOne SNP microrna StepOnePlus StepOne Fast 2 Fast 40 PCR Fast 10 Amplification Plot Allele Discrimination Plot (SNP Assay: SNP Assay 1) 4.1 3.6 3.1 ΔRn 1 Allele 2 2.6 2.1 1.6 1.1 0.1 0.6 0.1 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycle 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Allele 1 Homozygous 1/1 Heterozygous 1/2 Homozygous 2/2 Undeterminded 5 7. PCR StepOne 10 18S rrna TaqMan 10 log 8. SNP CYP2C19*2 TaqMan SNP Genotyping Assays StepOnePlus
VeriFlex 96 StepOnePlusPCR 6 VeriFlex VeriFlex PCR PCR VeriFlex PCR 6 VeriFlex VeriFlex 96 PCR VeriFlex StepOnePlus StepOne PCR StepOnePlus StepOne5,000 10,000 DNA 99.7 StepOnePlus StepOne 7 10 log 3.4 Melt Curve 10 Multiplex Assays 2.9 Derivative Reporter (-Rn ) 2.4 1.9 1.4.9 ΔRn 1 0.1.4 -.1 0.01 65.0 70.0 75.0 80.0 85.0 90.0 95.0 Tm: 79.2 Temperature ( C) 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 Cycle 9. PCR 18S GAPDH 3 Tm 10. StepOnePlus TaqMan 18S rrna VIC TGF- FAM cdna 48 6
2 < height: 51.8 cm > < depth: 48.5 cm > height: 51.8 cm > < depth: 48.5 cm > < < > width: 24.6 cm < > width: 24.6 cm StepOne 48 3 StepOnePlus StepOnePlus 96 4 VeriFlex 7
PCR / StepOnePlus StepOne PCR PCR Ambion Applied Biosystems RNA RNA Invitrogen RNA StepOnePlus StepOne PCR 1 StepOnePlus StepOne StepOne StepOnePlus TaqMan MicroRNA Assays microrna TaqMan Applied Biosystems TaqMan MicroRNA Assays MicroRNA Human MouseRatArabidopsis Drosophila C. elegans www. thermofisher.com/taqman TaqMan SNP Genotyping Assays Applied Biosystems TaqMan SNP Genotyping Assays 450 250 HapMap SNPs 70,000 csnps 160,000 2,500 Drug Metabolism Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP TaqMan SNP Genotyping Assays www.thermofisher.com/taqman Primer Express Applied Biosystems Primer Express v3.0 TaqMan PCR SYBR Green I Primer Express StepOnePlus StepOne 8
TaqMan Gene Expression Assays 700,000 PCR Applied Biosystems TaqMan Gene Expression Assays TaqMan Assays PCR PCR Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays www.thermofisher.com/taqman TaqMan Gene Expression, SNP Genotyping, and MicroRNA Assays TaqMan Assays Gene Expression* SNP Genotyping MicroRNA TaqMan Yes Yes Yes Inventoried Made-to-Order Custom TaqMan Assays Yes Yes No Human > 2,024,000 > 4,000,000 > 300 Mouse > 179,000 > 10,000 > 240 Rat > 128,000 > 180 Drosophila melanogaster > 38,000 > 50 Arabidopsis thaliana > 95,000 > 40 C. elegans > 90,000 > 60 Canine > 6,000 N/A Rhesus macaque > 70 N/A * mirna Gene copy number Mitochondrial HapMap drug metabolism genotyping SNPTaqMan 11. 9
TaqMan Fast Advanced Master Mix TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix 96 StepOnePlus/StepOne PCR Material List 11 StepOnePlus/StepOne PCR MicroAmp 48-Well Optical Adhesive Film 25 4375928 MicroAmp 48-Well Optical Adhesive Film 100 4375323 MicroAmp 96-Well Optical Adhesive Film 25 4360954 MicroAmp 96-Well Optical Adhesive Film 100 4311971 MicroAmp Fast Optical 48-Well Reaction Plate 20 4375816 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) 20 4346906 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) 200 4366932 8 MicroAmp Fast 8-Tube Strip, 0.1 ml 125 4358293 MicroAmp Optical 8-Cap Strip 300 4323032 MicroAmp Fast 48-Well Tray 10 4375282 MicroAmp 96-Well Tray for Veriflex Blocks 10 4379983 MicroAmp 48-Well Base Adaptor 5 4375284 Power SYBR Green PCR Master Mix 5mL 4367659 TaqMan Gene Expression Master Mix 5mL 4369016 TaqMan Genotyping Master Mix 10mL 4371355 TaqMan Fast Advanced Master Mix 5mL 4444557 TaqMan Fast Virus 1-Step Master Mix 200 4444432 SuperScript VILO cdna Synthesis Kit 50 11754050 10
Ordering information StepOne PCR StepOnePlus PCR StepOne-01 StepOnePlus-01 PCR www.thermofisher.com/qpcr www.thermofisher.com/tc For Research Use only.not for use in diagnostic procedures. 2016 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems. Printed in Japan. SDS024-E1602OB 108-0023 4-2-8 jptech@thermofisher.com 0120-477-392 TEL 03-6832-6980 FAX 03-6832-9584 TEL 03-6832-9300 FAX 03-6832-9580 facebook.com/thermofisherjapan www.thermofisher.com @ThermoFisherJP