Life Technologies NEXT 2011 CONTENTS New /Attune Attune Acoustic Focusing Cytometer P2 PRODUCT REVIEW 3100Mb! Ion PGM P6 10 No.9 E-Gel Go! & E-Gel Imager P3 MicroSEQ Ver.3.0 P7 microrna mirvana mirna mimics and inhibitors P4! P9 VILO SuperScript VILO Master Mix Gibco P5 P8 TECHNICAL REVIEW Qubit Protein Assay Kit PBS P10 SYBR Countess INFORMATION P5 P8 P12 P12 P12
Rat anti-mouse CD19 PE Cy5.5 Rat anti-mouse CD8 AF488 Mouse anti-mouse NK1.1 PE NEW /Attune Attune Acoustic Focusing Cytometer Acoustic focusing technology CV 10 * */ A 10 6 A. T and B Cell Subpopulations B 10 6 B. CD3+ gated CD4 and CD8 Subpopulations C 10 6 C. CD11 and NK1.1 Subpopulations 10 5 10 5 10 5 10 4 10 4 10 4 10 3 10 3-10 3-10 3-10 3 10 3 10 4 10 5 Hamster anti-mouse CD3e Alexa Fluor 647 10 3-10 1-10 3-10 3 10 3 10 4 10 5 10 6 Rat anti-mouse CD4 PE-Cy 7-10 3 10 4 10 5 10 6 Rat anti-mouse CD11c APC-Cy 7 1. / Attune Acoustic Focusing Cytometer6 C57BL/6J6 (A) CD3e Alexa Fluor 647CD19 PE-Cy 5.5 (B) CD4 PE-Cy 7CD8 Alexa Fluor 448 (C) CD11c APC-Cy 7NK1.1 PE CD11b PE BL2-H GR-1 Pacific Orange VL3-H 2. 3. Pro Syn Euk Fluospheres Attune Acoustic Focusing Cytometer / Attune Acoustic Focusing Cytometer, RED LASR / 1 4445315 1 4469120 2
NEW E-Gel Go! & E-Gel Imager E-Gel Go! E-Gel ImagerE-Gel Blue Light UV E-Gel Go! 10μl 4 15 1% 2% 2 50bp 2kb E-Gel EX 1ng DNA E-Gel Go! Base 82X125X43mm E-Gel Go! 1% Gels 10pack G441001 6,000 E-Gel Go! 1% Gels 20pack G442001 10,000 E-Gel Go! 2% Gels 10pack G441002 6,000 E-Gel Go! 2% Gels 20Pack G442002 10,000 E-Gel Go! Base 1 G4400 75,000 E-Gel Go! 1% Starter Kit 1Kit G4401ST 80,000 E-Gel Go! 2% Starter Kit 1Kit G4402ST 80,000 E-Gel Go! Portable Battery Pack 1 G4405 40,000 E-Gel Go! Car Adaptor 1 G4444 12,000 E-Gel Go! Travel Kit G4405 G4444 1Kit G4400TK 50,000 Starter kit10base 1kb Plus DNA E-Gel Imager 3 Universal UV/SYBR Qdot 625 E-Gel Go!E-Gel EX E-Gel 48 E-Gel 96 E-Gel Imager UV Light Base 1 4466602 128,000 E-Gel Imager Blue Light Base 1 4466603 127,000 E-Gel Imager Adaptor Base 1 4466604 50,000 E-Gel Imager Band Excision Kit 1Kit 4466605 40,000 E-Gel Imager Universal Filter 1 4466606 30,000 E-Gel Imager Qdot 625 Filter 1 4466607 30,000 E-Gel Imager UV/SYBR Filter 1 4466608 30,000 E-Gel Imager Quantitation Dongle 1 4466610 30,000 E-Gel Imager with UV Light Base 1 4466611 700,000 E-Gel Imager with Blue Light Base 1 4466612 700,000 E-Gel Imager with E-Gel Adaptor 1 4466613 600,000 4466604 4466613E-Gel Go! BaseE-Gel ibase/e-gel Safe Imager 35.6cm X30.5cm X21cm 100-240V,50/60Hz,0.6A 540 UV Light/Blue Light Base 11.9cm X30.5cm X21cm 100-240V,50/60Hz,0.6A 540 3
NEW microrna mirvana mirna mimics and inhibitors mirvana mirna mimics and inhibitorsmirna mirna mirvana mirna mimics mirna mirnamirna mirvana mirna inhibitors mirnamirna Sanger Database, in vivoin vivo ready, Fold Change 1.40 1.20 1.00 0.80 0.60 0.40 0.20 0.00 mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic Neg mirna mimic reporter 1 reporter 2 reporter 3 reporter 4 reporter 5 reporter 6 FW RV Neg mrna upregulation % 450 400 350 300 250 200 150 100 50 0 mir 122 mir122 mir122 mirna inhibitor NT Not transfected Neg1 Negative Control #1 NT Neg1 mir NT Neg1 mir NT Neg1 mir NT 122 122 122 AldoA Hfe2 Slc35a4 Lass6 Neg1 1 mirvana mirna mimic microrna mirvana mirna mimic microrna mirvana mirna mimic mirna mimic 1 microrna microrna 6 5-10 2 mirvana mirna Inhibitors in vivo mirna Balb-C mirvana mirna inhibitor mir122negative Control in vivoinvivofectamine 2.07 mg/kg 3 mirna inhibitor 4 AldoA, Hfe2, Slc35a4 and Lass6 mir122 mirna inhibitor Negative control mrna AldoA 3.5, Hfe2 2, Slc35a4 3, Lass6 4 mirvana mirna inhibitor Invivofectamine 2.0 Reagent mir122 mir122 mirvana mirna mimic mirvana mirna mimic 5nmol 4464066 58,000 mirvana mirna mimic 20nmol 4464067 178,000 mirvana mirna mimic, in vivo Ready 250nmol 4464070 510,000 Customer-defined mirvana mirna mimic 5nmol 4464068 98,000 Customer-defined mirvana mirna mimic 20nmol 4464069 180,000 Customer-defined mirvana mirna mimic, in vivo ready 250nmol 4464071 510,000 mirvana mirna mimic, Custom 20nmol 4464072 mirvana mirna mimic, Custom 250nmol 4464073 mirvana mirna mimics Library* 0.25nmol each 4464074 mirvana mirna mimics Library* 1nmol each 4464075 mirvana mirna inhibitor mirvana mirna inhibitor 5nmol 4464084 58,000 mirvana mirna inhibitor 20nmol 4464085 178,000 mirvana mirna inhibitor, in vivo ready 250nmol 4464088 510,000 Customer-defined mirvana mirna inhibitor 5nmol 4464086 98,000 Customer-defined mirvana mirna inhibitor 20nmol 4464087 180,000 Customer-defined mirvana mirna inhibitor, in vivo ready 250nmol 4464089 510,000 mirvana mirna inhibitor, Custom 20nmol 4464090 mirvana mirna inhibitor, Custom 250nmol 4464091 mirvana mirna inhibitor Library* 0.25nmol each 4464092 mirvana mirna inhibitor Library* 1nmol each 4464093 Negative Control, Positive Control * Sanger database v17 mirna 4 mirvana mirna mimics and inhibitors http://www.appliedbiosystems.jp/website/jp/store/pages.jsp
NEW VILO SuperScript VILO Master Mix SuperScript VILO Master MixVILOVILO RNA RNA cdna PCR 3 4 SuperScript PCR RNA 1pg 2.5μg 5020μl 11755050 38,000 SuperScript VILO Master Mix 25020μl 11755250 160,000 50020μl 11755500 290,000 SYBR TaqMan TaqMan 38,500 SYBR SYBR 15,000 PCRTaqMan? Fast SYBR Power SYBR TaqMan 50% 50% OFF 2011.9.20 2011.10.28 Fast TaqMan Fast Advanced Master Mix P2-XD7WEV Fast SYBR 4385612CAMP 77,000 38,500 Fast SYBR Green Master Mix,5mL 1 500TaqMan Fast Advanced Master Mix, 1mL 1100 TaqMan GeneExpression Assays, Extra Small 275* 20μL Power SYBR 4367659CAMP 77,000 38,500 Power SYBR Green Master Mix,5mL 1 500TaqMan Fast Advanced Master Mix, 1mL 1100 TaqMan GeneExpression Assays, Extra Small 275* 20μL **TaqMan http://www.appliedbiosystems.jp/! RNA PureLink RNA Mini kit RNA Qubit 2.0 Fluoromter http://www.invitrogen.jp 5
PRODUCT REVIEW 3100Mb! Ion PGM Ion PGM 1 Ion PGM 10Mb10100Mb 12011200bp 2012400bp denovo RNA 314 JAN 2011 10Mb 100bp 316 JUL 2011 100Mb 100bp 318* Q4 2011 1Gb 200bp Ion PGM Ion PGM 1 Ion Xpress Fragment Library Kit Ion OneTouch DNA Duo PCR 60% 3.5 70% 4 Ion PGM 30 bp 1 100bp 2 Torrent Suite v 1.4 Ion 314 chip 30Ion 316 chip 60 WORKFLOW TIMES 3.5 HOURS 3.5 HOURS Only minutes of hands-on time 2.5 HOURS Under one hour for short reads 1.0 HOURS The PGM takes the shortest time to generate genomic data. JUNJIE QIN BGI, CHINA ION KITS ION ONE-TOUCH SYSTEM ION PGM SEQUENCER TORRENT SERVER Ion PGM Nature 7 21NatureIon PGM Ion PGM CMOS IC 3 Postlight 2 O104 H4 Ion Personal Genome Machine Nature * www.appliedbiosystems.jp/website/pgm/nd.html 2011 10 21 6
PRODUCT REVIEW MicroSEQ Ver.3.0 MicroSEQ 4Version 3.0 MicroSEQ 3500 / 5 Specimen 211 0.6467% 211 and Curvibacter lanceolatus* ATCC 14669 Genetic Distance 0.31% Hydrogenophaga pseudoflava* ATCC 33668 Hydrogenophaga taeniospiralis* ATCC 49743 Hydrogenophaga flava* ATCC 33667 Hydrogenophaga palleronii* ATCC 17724 Malikia spinosa* ATCC 14606 Acidovorax konjaci* ATCC 33996 Variovorax paradoxus* ATCC 17713 211 Curvibacter lanceolatus* ATCC 14669 MicroSEQ 3500 BLAST MicroSEQ 4000! 5 16S 500 2020 16S 1262 D2 1114 100 2011 9 Fast PCR 4370653 4346479 PCR 4396787 4347482 MicroSEQ DNA MicroSEQ V3.0 BUNDLE 1 4448336 5,250,000 MicroSEQ V3.0 BUNDLE 3 4467661 7,980,000 MicroSEQ MicroSEQ V3.0 BUNDLE LITE 1 4467862 4,250,000 MicroSEQ V3.0 BUNDLE LITE 3 4467863 6,000,000 MicroSEQ MicroSEQ v2 MicroSEQ V3.0 3500 4452803 1,980,000 MicroSEQ V3.0 3130 4448335 1,980,000 5003 5 7
NEW Gibco Gibco ISO13485 www.invitrogen.com/growthfactors www.invitrogen.com/stemcell IGF-1, Recombinant Human 20μg PHG0078 14,000 IGF-1, Recombinant Human 100μg PHG0071 28,000 LIF, Recombinant Human 1000μg PHC9463 749,000 IL-9, Recombinant Human 10μg PHC0094 32,000 IL-9, Recombinant Human 100μg PHC0091 277,000 IL-28A, Recombinant Human 10μg PHC0284 28,000 IL-28B, Recombinant Human 10μg PHC0894 28,000 LEFTY, Recombinant Human 10μg PHC1714 19,000 HGF, Recombinant Human 10μg PHG0324 32,000 HGF, Recombinant Human 100μg PHG0321 277,000 CYSTATIN C, Recombinant Human 10μg PHP0044 15,000 HGH, Recombinant Human 10μg PHH0014 20,000 Countess 12 22 30 www.invitrogen.com/countess 100 P789274 Countess 50Trypan Blue 0.4% 2 ml 1 C10227 625,000 450,000 Countess Countess 2 Trypan Blue 0.4% 50 C10228 11,000 500 C10312 99,000 1,250 C10313 235,000 2,500 C10314 460,000 5,000 C10315 880,000 8
PRODUCT REVIEW! Gateway Gateway BP, LR 1kbp 230,000 3 TOPO TOPO 230,000 3 250,000 4 Gateway Gateway 50 5 Gateway Gateway 200,000 4 160/bp 3 63,000 2 T7, pbad 500,000 5L 2 Bac-to-Bac 1,600,000 1L 7 FreeStyle FreeStyle 293 650,000 5L 1x10e4 10e6 TU/ml 560,000 6 1x10e8 PFU 680,000 7 BacMam 9,000 1,400,000 10 cdna total RNA 900,000 8 SelectScreen Lantha Screen Eu kinase Binding Assay SelectScreen Select Screen herg 250 IC50 ATP GPCR QT herg 5-100 mg 15 5 2 3,400/ 5mg, 90% 287,000 2 2 2 2 12 TEL 03-5753-3006 FAX 03-5753-3007 E-mail jpcustom invitrogen.com 9
TECHNICAL REVIEW Qubit Protein Assay Kit PBS QubitDNA RNA Qubit Protein Assay Kit Qubit Protein Assay Kit Qubit 2.0 Fluorometer Qubit 2.0 Fluorometer 3 Qubit 1 PBS PBSPBS TE buffer PBS5 20μL 200ul Qubit PBS PBS RFU 900 800 700 600 500 400 300 200 100 0 Water TE buffer PBS 5 μl 10 μl 20 μl 2 PBS Qubit Protein Assay TE buffer BSA PBS PBSPBS QubitPBS 0 μl TE buffer PBS PBS μg/ml 25 20 15 10 5 0 Sample A Sample B Sample C PBS 0 μl PBS 5 μl PBS 10 μl 3 Triton X-100 QubitSDS 0.2% Triton X-100 Tween 20 0.02%* Triton X-100 * 10μl Triton X-100 0.1% milli-q Triton X-100 Triton X-100 Triton X (RFU) 50000 45000 40000 35000 30000 25000 20000 15000 10000 5000 0 0 0.002 0.003 0.006 0.013 0.025 0.05 0.1 10μl Triton X-100 (%) 0 μg/ml 25 μg/ml 50 μg/ml 100 μg/ml 200 μg/ml 400 μg/ml 10
TECHNICAL REVIEW 4 Triton X-100 PBSQubit Protein Assay Triton X-100 Triton X10μl Triton X 10μl Qubit Triton X-100 Triton X Triton X 0.013% Qubit "Standard Incorrect" Triton X Qubit μg/ml 450 400 350 300 250 200 150 100 50 0 Sample 0 Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 TritonX 0 % 0.002 % 0.003 % 0.006 % Qubit Qubit Protein Assay Kit PBS 10μlTriton X-100 0.006% Qubit 2.0 Fluorometer 1 Q32866 170,000 100 Q33211 9,900 Qubit Protein Assay Kit 500 Q33212 32,700 Qubit Assay Tubes 500 Q32856 6,000 Qubit, Protein Assay Kit 100, Tube 1 Q33211NSK 180,000 Q.?? A. Q.? A. BSA Q.? A. DTT 2-mercaptoethanol 11
INFORMATION 70 10 35 http://www2.convention.co.jp/70jca/ American Society for Human Genetics ASHG 10 1214 Palais des Congres de Montreal http://www.ichg2011.org/ 56 11 11 912 http://www.jshg-56.jp/ http://www.appliedbiosystems.jp/website/jp/event/eventpage.jsp http://www.lifetechnologies.co.jp/sem/index.html QUIZ Life Technologies! 10Life Technologies Q? A! http://www.lifetechnologies.co.jp/quiz.htm 2011 10 24 9 40 2008 Invitrogen Corporation Applied Biosystems Inc Invitrogen Applied Biosystems PCR DNA http://www.lifetechnologies.co.jp 2011 9 The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. 2011, Life Technologies Japan Ltd. All rights reserved. Printed in Japan. 108-00234-2-8 TEL.03 6832 9300 FAX. 03 6832 9580 http://www.appliedbiosystems.jp http://www.invitrogen.jp