Discover Life Science 216 1 11 216 12 22 Products and Services P2 5 P6 7 P811 P926 P1 P12 14 P15 P16 17 P18 PCRP19P22 24 P2 PCRP21 P25 RNAiP27 Vol.8
ProLong / SlowFade 3%OFF Invitrogen ProLong SlowFade Invitrogen Molecular Probes 24 6 ProLong Diamond ProLong Gold 34 SlowFade Diamond SlowFade Gold ProLong Diamond ProLong Gold 5%PBS Glycerol ProLongFITC 6ProLong HeLa FITC-phalloidinF432ProLong DiamondProLong Gold5%PBS/Glycerol 12 12 12 24 36 48 6 Invitrogen ProLong Live Antifade GFP HeLa U2OS 2 Em / Ex 15 Thermo Scientific ArrayScan VTI HCS Reader Cellomics Invitrogen LIVE / DEAD Red R3761 component B Invitrogen Click-iT Plus EdU C1639 Hoechst 33342 1 % ControlMedia only 6 Prolong Live in Media 1 ControlMedia only emgfp MitoTracker Green TagRFP MitoTracker Red MitoTracker Deep Red 6 35 25 35 25 8 8 7 55 6 12 8 7 7 6 19 18 14 12 12 Hoechst 3334275 8 Prolong Live in Media 23 % of Live Cells 1 8 6 4 2 Control 6 hours 12 hours 18 hours Time (Hours) 24 hours 3 hours 36 hours 42 hours 48 hours % of Proliferating Cells 96-Well 1 /HeLa 1%FBS MEM ProLong Live Antifade 6 48 8 LIVE / DEAD Red Click-iT Plus EDU ArrayScan VTI HCS Reader = 1 8 6 4 2 Control 6 hours 12 hours 18 hours 24 hours Time (Hours) 3 hours 36 hours 42 hours 48 hours 2
ProLong / SlowFade Invitrogen Image-iT FX Signal Enhancer / Ready-to-use Invitrogen BlockAid Blocking Solution BSA 5µg/mLInvitrogen Alexa Fluor 488 Goat Anti-Mouse IgG A1129 6% bovine serum albuminbsa5% normal goat serumngsinvitrogen BlockAid Blocking SolutionBlock-AidB171 1 ProLong Diamond Antifade Mountant ProLong Diamond Antifade Mountant with DAPI ProLong Gold Antifade Mountant ProLong Gold Antifade Mountant with DAPI SlowFade Diamond Antifade Mountant SlowFade Diamond Antifade Mountant with DAPI SlowFade Gold Antifade Mountant SlowFade Gold Antifade Mountant with DAPI ProLong Live Antifade Reagent, for live cell imaging Image-iT FX Signal Enhancer Image-iT FX Signal Enhancer ReadyProbes Reagent BlockAid Blocking Solution RE4T22 Alexa Fluor 488 Streptavidin staining of BPAEC cells BSA NGS BlockAid 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 2 ml 5 2 ml 1 ml 1 ml 5 1 ml 1 ml 1 kit 5 ml P36965 P36961 P3697 P36966 P36962 P36971 P1144 P36934 P3693 P36941 P36935 P36931 S36967 S36963 S36972 S36968 S36964 S36973 S3694 S36937 S36936 S36942 S36939 S36938 P36975 P36974 I36933 R3717 B171 Alexa Fluor 488 Streptavidin with Image-iT FX Signal Enhancer Invitrogen Image-iT FX Signal Enhancer 7, 3,7 27,2 8,2 32,2 29,9 7, 3,7 27,2 8,2 32,2 29,9 7, 26,4 22,8 8,2 28,5 25,4 7, 26,4 22,8 8,2 28,5 25,4 8, 28,3 16,6 26,9 22,2 4,9 5,7 4,9 5,7 4,9 5,7 4,9 5,7 5,6 11,6 18,8 15,5 216111 2161222 3
Invitrogen EVOS FL 2,364,... 1,9, EVOS FL, Monochrome Camera AMF43 Power Cord -EVOS FL AMEP4644 Plan Achro4X PH,.13NA /16.9WD AMEP4632 LED Light Cube, GFP AMEP4651 Vessel Holder for Slides, Holds two 25mm 75mm AMEPVH1 www.thermofisher.com/evos REEKM3 216111 2161222 EVOS FL Auto 2 Imaging System! Invitrogen EVOS FL Auto 2 Imaging System Z-? www.thermofisher.com/evosflauto2 4
!! 1 Invitrogen Countess II! 1 33% OFF 1µL1 5 Countess II 5 54,... 36, Countess II Automated Cell Counter Countess Cell Counting Chamber Slides 1 5 1 5 AMQAX1 C1312 45, 9, 36, 1 www.thermofisher.com/countess REBC3E 216111 2161222 4 EVOS FL Auto 2 Imaging System : 4 xph1xfi2xfi4xfi : GFPRFPDAPI XY : & 7,5, EVOS FL Auto EVOS Onstage Incubator EVOS FL Auto AMC1 1,75, 5
DNARNA Qubit 3 Invitrogen Qubit 3 Fluorometer Qubit 32,5 Q32856 5 Qubit 3 Fluorometer LED ODDNARNA 8 7 6 5 4 3 2 1 4,61 3,475 2,73 975 Qubit dsdna HS Assay CV 7 6 5 4 3 2 1 1 Qubit dsdna HS Assay.1.2.3.1.5 1 2 4 6 8 1 [DNA]ng / µg.5 ng / µl DNA % CV1%.1.2.3.1.5 1 2 4 6 8 1 [DNA]ng / µg Result in ng/µl 14 12 1 8 6 4 2 Quant-iT dsdna BR Assay Quant-iT RNA BR Assay A B 1: 1:5 1:1 1:2 1:3 1:4 1:5 1:75 1:1 DNA:RNA rationg / µl PCR Qubit Fluorometer 1 ng / µlribosomal E. coli RNA 1ng / µllambda DNA Qubit FluorometerDNA RNA Qubit 3. Fluorometer 1 Q33216 21, Qubit dsdna BR Assay Kit,21, ng 1 assays Q3285 1,9 5 assays Q32853 36,1 Qubit dsdna HS Assay Kit,.21 ng 1 assays Q32851 1,9 5 assays Q32854 36,1 Qubit Protein Assay Kit,.255 µg 1 assays Q33211 1,9 5 assays Q33212 36,1 Qubit ssdna Assay Kit,12 ng 1 assays Q1212 1,9 MSAH3E 216111 2161222 6
/ 7 Qubit RNA BR Assay Kit,21, ng 1 assays Q121 1,9 5 assays Q1211 36,1 Qubit RNA HS Assay Kit,51 ng 1 assays Q32852 1,9 5 assays Q32855 36,1 Qubit microrna Assay Kit,11ng 1 assays Q3288 1,9 5 assays Q32881 36,1 Qubit Assay Tubes 5 tubes Q32856 6,7 5 7
Lipofectamine 3 Lipofectamine 3 Invitrogen Lipofectamine 3 Reagent Gibco Invitrogen Lipofectamine 3 Reagent 1DNARNAco-transfection Gibco TM Invitrogen TM Gibco Opti-MEM Invitrogen PureLink Gibco One Shot FBS 5mL x 1 Lipofectamine 3 Lipofectamine 3 Lipofectamine 3 Lipofectamine 3 Reagent.1 ml L31 9,9 Opti-MEM 1 ml3198562.75 ml L38 7,8 PureLink K214 1.5 ml L315 16,4 PureLink K214 5 1.5 ml L375 471,3 One Shot FBS 1 x 5 mla31652 15 ml L315 789,2 One Shot FBS 1 x 5 mla31652 1,9 Opti-MEM I Reduced Serum Medium 1 ml 3198562 1,9 PureLink HiPure Plasmid Midiprep Kit 25 K214 29,3 FBS, qualified, One Shot format, US origin 1 x 5 ml A31652 148,5 & DNA sirna mrna Cas9 Protein Lipofectamine 2 Lipofectamine 3 DNA DNA sirna sirna DNAsiRNA DNAsiRNA Lipofectamine RNAiMAX sirna microrna Lipofectamine MessengerMAX mrna Lipofectamine CRISPRMAX Cas9 Protein Neon Transfection System DNA sirna microrna.1 ml.1 ml.1 ml.1 ml L31 137781 LMRNA1 CMAX1 MRAX22 MSBT22 216111 2161222 216711 216923 8
GeneArt Platinum Cas9 Nuclease Invitrogen GeneArt Platinum Cas9 Nuclease 7% CRISPR HEK293FT U2OS ES ES (H9) ips N2A Jurkat K562 A549 Plasmid GCD% mrnagcd% ProteinGCD% Lipofectamine 3 49 15 3 66 15 Lipofectamine MessengerMAX 7 21 45 2 66 66 23 Lipofectamine CRISPRMAX 75 55 75 n/a 55 7 19 2 48 DNAmRNA Invitrogen GeneArt Genomic Cleavage Detection Kit GCD% Invitrogen Lipofectamine CRISPRMAX Cas9 Transfection Reagent CRISPR Cas9 Nuclease GeneArt Platinum Cas9 Nucrease Cas9 Nuclease & grna ipses Gibco Human ipsh9 ESGeneArt Cas9/gRNARNP 48 72GeneArt Genomic Cleavage Detection Kit GeneArt Platinum Cas9 Nuclease 1 Cas9 Protein 1 Lipofectamine CRISPRMAX Lipofectamine 3 Neon CRISPR DNA CRISPR CRISPR mrna CRISPR protein Lenti-Viral production Lipofectamine CRISPRMAX Cas9 Transfection Reagent Lipofectamine MessengerMAX GeneArt Platinum Cas9 Nuclease 1 µg 1 µg / µl B25642 43, 25 µg 1 µg / µl B2564 85, 75 µg 3 µg / µl B25641 18, Lipofectamine CRISPRMAX Cas9 Transfection Reagent 1 CMAX1 1,8 GeneArt Precision grna Synthesis Kit grna! 25 A29377 87, GeneArt Genomic Cleavage Detection Kit! 2 A24372 48,1 MSF32A 216111 2161222 9
ipses TaqMan hpsc Scorecard Panel Applied Biosystems TaqMan hpsc Scorecard Panel Kit 2 RNA Invitrogen TRIzol Reagent 1 ml1 Applied Biosystems TaqMan hpsc Scorecard Panel hpsc PCR 93 Applied Biosystems PCR7 H9-ESC-...5-Day Selfrenewal Ectoderm Mesoderm Endoderm H9-EB-Day7 Selfrenewal Ectoderm Mesoderm Endoderm Upregulated Downregulated > 1 1 < fc 1 2 < fc 1.5 fc 2.1 fc <.5.1 fc <.1 fc <.1 mit 7PCR TaqMan hpsc Scorecard Panel 2 QuantStudio 12K Flex ViiA 7 StepOnePlus 79 HT TRIzol Reagent 1 QuantStudio 7 QuantStudio 6 75 Fast TaqMan hpsc Scorecard Panel TaqMan hpsc Scorecard Kit, Fast 96-Well 2 x 96-Well A15871 96, TaqMan hpsc Scorecard Panel, Fast 96-Well 2 x 96-Well A15876 7,5 TaqMan hpsc Scorecard Kit, 384-Well 1 x 384-Well A15872 89, TaqMan hpsc Scorecard Panel, 384-Well 1 x 384-Well A1587 5,6 TRIzol Reagent 1 ml 1559626 28,3 MSPA4A 216111 2161222 1
DNA mrna Lipofectamine MessengerMAX Invitrogen mmessage mmachine T7 ULTRA Transcription Kit AM1345 mrna Invitrogen Lipofectamine MessengerMAX Transfection Reagent.1 ml Invitrogen Lipofectamine MessengerMAX Reagent DNA Lipofectamine MessengerMAX mrna Reagent hnsc BJ RAW 264.7 1 2 mrna3 DNA T7DNA DNAInvitrogen Gateway pcdna -DEST4 T7PCR mrna mmessage mmachine T7 ULTRA Transcription Kit DNAmRNA DNA mrna 3 ARCA-capped mrna with polyatail mrna Lipofectamine MessengerMAX mrna 1. Attachment to cell 2. Endocytosis into cell 3. Escape from endosome 4. Nuclear entry Protein expression Reagent DNA complex DNA mrna Endosome DNA Nucleus Protein mmessage mmachine T7 ULTRA TranscriptionKit 1 Lipofectamine MessengerMAX.1mL 1 Reagent mrna complex mrna DNA vs mrna Lipofectamine MessengerMAX mrna mrnadna 4 mmessage mmachine T7 ULTRA Transcription Kit 1 AM1345 96,8 Lipofectamine MessengerMAX Transfection Reagent.1 ml LMRNA1 1,4.3 ml LMRNA3 3,.75 ml LMRNA8 72,6 1.5 ml LMRNA15 13,1 mrna MSHCM3 216111 2161222 11
25% OFF Invitrogen MagicMark XP Western Protein Standard IgG Invitrogen Novex Sharp Protein Standard Invitrogen HiMark Protein Standard 25kDa MagicMark XP Western Protein Standard AB kda 26 16 11 8 6 5 4 3 2 15 1 3.5 Novex Sharp Pre-stained LC58 kda 188 98 62 49 38 28 17 14 kda 188 62 49 38 28 18 14 kda ~19 ~12 ~85 ~6 ~5 ~4 ~25 ~2 ~15 kda 46 268 238 171 117 71 55 6 6 ~1 41 6 2 3 3 3.5 3.5 31 2.5 4 SeeBlue SeeBlue BenchMark HiMark Novex Sharp BenchMark Mark12 HiMark Plus2 Pre-stained Pre-stained LC5925 LC5625 17481 LC5699 LC581 174712 LC5677 LC5688 kda 26 16 11 8 6 5 4 3 2 15 1 kda 22 16 12 1 9 8 7 6 5 4 3 25 2 15 1 kda 2 116.3 97.4 66.3 55.4 36.5 31 21.5 14.4 kda 5 29 24 16 116 97 66 55 kda 22 12 1 8 6 5 4 3 MagicMark XP LC562 / LC563 Novex Sharp Pre-stained Protein Standard 2 25 µl LC58 25,9 19,4 SeeBlue Plus2 Pre-stained Protein Standard 5 µl LC5925 24,8 18,6 SeeBlue Pre-stained Protein Standard 5 µl LC5625 24,8 18,6 BenchMark Pre-stained Protein Ladder 2 25 µl 17481 22,6 16,9 HiMark Pre-stained Protein Standard 25 µl LC5699 31,8 23,8 Novex Sharp Unstained Protein Standard 2 25 µl LC581 24,2 18,1 BenchMark Protein Ladder 2 25 µl 174712 22,6 16,9 Mark12 Unstained Standard 1 ml LC5677 21,3 15,9 HiMark Unstained Protein Standard 25 µl LC5688 29,3 21,9 MagicMark XP Western Protein Standard 25 µl LC562 37,5 28,1 5 µl LC563 11,4 8,5 RETP25 216111 2161222 12
Bradford Pierce 66nm Protein Assay 25% OFF Thermo Scientific Pierce 66nm Protein Assay - Bradford Bradford 1566 nm 22662 BSA (66 nm) 3 2 1 Thermo Scientific Pierce 66nm Protein Assay Reagent B Protein Assay Bradford Ionic Detergent Compatibility Reagent 5 1, 1,5 2, 2,5 BSA (µg/ml) Pierce 66 nm Protein Assay Reagent Bradford Protein AssayB (66 nm) BSAPierce 66 nm Protein Assay 25-2, µg/ml Bradford Assay125-1, µg/ml Pierce 66nm Protein Assay Reagent 55, 75 ml 2266 32,8 24,6 Pierce 66nm Protein Assay Kit 33, 1 kit 22662 46,4 34,8 : Pierce 66 nm Protein Assay Reagent 45 ml Pre-Diluted Protein Assay Standards, Bovine Serum Albumin Set 7 x 3.5 ml Ionic Detergent Compatibility Reagent for Pierce 66nm Protein Assay Reagent 1Pierce 66 nm Protein Assay Reagent 2 ml5 5 1 g 22663 21,4 16, REER45 216111 2161222 ELISA 8 ELISA ELISA3 www.thermofisher.com/findelisa 13
3 Invitrogen Mini Gel Tank 65,9 3 Mini Gel Tank Mini Gel Tank A25977 65,9 1 2 3 3 Mini Gel Tank Get www.thermofisher.com/jp-geltank WedgeWell Tris-Glycine GelsBolt GelsNuPAGE Gels 11 WedgeWell Tris-Glycine Gels Bolt Gels NuPAGE Gels 6 35 35 Mini:88cm 1mm1.5mm Mini:88.3cm 1mm Mini:88cm 1mm1.5mm Midi:813cm 1mm 12 16 8Tris-Acetate 16Bis-Tris 1 1 1 3 16 Mini:34 Midi:21 www.thermofisher.com/proteingels 3 216111 2161222 14
CTC LiquidBiopsy Platform CTC EpCAM EMT 7.5-1 ml CTC cfdna, Germline ControlWhite Blood Cell 96CTC www.thermofisher.com/jp-liquidbiopsy CTC Ion Torrent LiquidBiopsy Instrument 8-1% (3-5cells) 22 2 cells / ml CTC CTC molecular analysis / 4 Iohexol PBS CTC 82cm 82cm 58cm LiquidBiopsy Instrument2 1 CVLB1-1 19,5, LiquidBiopsy Blood Collection Kit 1 A28171 9,4 LiquidBiopsy Reagents and Consumables Kit,Part 1 8 A28186 142,8 LiquidBiopsy Reagents and Consumables Kit,Part 2 8 A325 282, EpCAM Capture Antibody 8 A32596 9, Her2 Capture Antibody 8 A32597 9, Trop2 Capture Antibody 8 A32598 9, Bi LabelEpCAM+Trop2Capture Antibodies 8 A32599 9, Bi LabelEpCAM+Her2Capture Antibodies 8 A326 9, Bi LabelHer2+Trop2Capture Antibodies 8 A3261 9, Tri LabelEpCAM+Her2+Trop2Capture Antibodies 8 A3262 9, 15
RNA RNA 2%OFF RNARNase RNA RNase l M / Hind lll Markers A B R RNaseZap Invitrogen RNaseZap Decontamination Solution RNase RNase RNase 28S rrna 18S rrna RNase RNA RNA RN RNA Invitrogen RNAlater Stabilization Solution 37 1 1 4 1 RNA RNaseRNA 28S rrna 37125141-2RNA 18S rrna Invitrogen RNAlater -ICE Frozen Tissue Transition Solution RNA RNA RNA RNAlater Solution RNA 5 RNAlater Solution 371251 41p53 GAPDH RNAlater Solution RNA p53 GAPDH 16
RNA RNA Invitrogen TRIzol Reagents Invitrogen PureLink RNA Mini Kit Invitrogen TRIzol Reagent DNA RNA RNA Invitrogen Purelink RNA Mini Kit 21, µg RNA PureLink RNA Mini Kit Competitors midi kit Competitors mini kit PureLink RNA Mini Kit Q 25 mg1 mg2 mg RNA RNaseZap RNase Decontamination Solution RNaseZap RNase Decontamination Wipes Refill RNAlater Stabilization Solution RNAlater -ICE Frozen Tissue Transition Solution PureLink RNA Mini Kit 1,µg PureLink RNA Micro Scale Kit 1µg TRIzol Reagent TRIzol Plus RNA Purification Kit 1559626 1218318A TRIzol LS Reagent TRIzol Max Bacterial RNA Isolation Kit 25 ml 6 25 ml 1 3 1 ml 5 ml 5 1.5 ml 2 5 ml 1 25 ml 25 ml 1 5 25 5 1 ml 2 ml 5 1 ml 2 ml 1 2 AM978 AM9782 AM9786 AM9788 AM72 AM721 AM722 AM723 AM724 AM73 121832 1218318A 1218325 1218316 1559626 1559618 12183555 12961 129628 16962 16964 12,5 56,4 12,5 33,8 18,6 6,1 44,5 44,5 34,6 19,3 1,7 32,9 138, 46, 28,3 5,1 57,8 28,1 49,5 37,3 55,8 1, 45,1 1, 27, 14,8 48, 35,6 35,6 27,6 15,4 8,5 26,3 11,4 36,8 22,6 4, 46,2 22,4 39,6 29,8 44,6 RECHM3 216111 2161222 17
1 SuperScript IV 3% OFF Invitrogen SuperScript III Reverse Transcriptase Invitrogen SuperScript IV Reverse Transcriptase 1 RNARNAcDNA RNA 55RNA RNARNA A 25S/28S 18S 5S RIN >8 RIN 1 3 Flax seeds Wheat germ B Normalized RT efficiency.8.6.4.2 SSIII SSIV P T BR Q N EF1A Wheat germ SSIII SSIV P T BR Q N ETIF5A Flax seeds A : R N A 5ng1.2% Invitrogen E-Gel RNARNARIN 82Agilent Bioanalyzer RIN13 RNA B: RNART-qPCR RNA1ng2µL SuperScript IV RT RT c D N A 1 % Invitrogen EXPRESS qpcrsupermix, Universal 117851K EF1AETIF5A TaqMan CT SuperScript IV RT SuperScript IV Reverse Transcriptase 2, units 1891 13,1 9,1 1, units 1895 53,7 4 1, units 1892 17, REF72A 216111 2161222 18
定量PCR 高パフォーマンスのリアルタイムPCR用 cdna合成システム登場 SuperScript IV VILO Master Mix 今ならRNA精製 PureLink RNA Mini Kitがもらえるキャンペーン 期間中 Invitrogen SuperScript IV VILO Master Mixをご購入いただくと もれなくInvitrogen PureLink RNA Mini Kit 1回分 をプレゼントいたします SuperScript IV VILO Master Mixは 2ステップのリアルタイム定量PCR RT-qPCR 用にデザインされた新しいcDNA合成試薬です 高いDNA鎖伸長能 processivity と熱安定性を持つ新しい酵素 Invitrogen SuperScript IV Reverse Transcriptaseを採用することで 純度が低いサンプル や微量のテンプレートでも 高いcDNA合成収量および感度を提供します 4つのSuper! 今なら もれなく プレゼント 高速 逆転写反応はわずか1分 gdna除去は2分 で完了します 高収量 テストしたすべての他社試薬より 2サイクル以上早く増幅します 高感度 微量なRNAや純度の低いサンプルにも対応しています PureLink RNA Mini Kit は カラムべースの RNA 精製キットで す 約 2 分で最大 1,µg のトータル RNA を精製でき 様々な サンプルに対応しています 製品キャンペーン実施中 17 ページを ご覧ください 簡便 逆転写反応に必要な成分が1本のチューブに入った マスターミックスです ezdnase付き 製品番号117665 117665 をお買い求めください 高い感度で逆転写困難なRNA分解したサンプルにも対応 SuperScript IV VILO Master Mix SuperScript VILO Master MIx 他社製品 1 他社製品 2 他社製品 3 他社製品 4 他社製品 5 他社製品 6 他社製品 7 Normalized RT efficiency to SSIV VILO 1.2 1.8.6.4.2 No Inhibitor 75 mm LiCl.3% TRIzol.4% SDS.7 U/µL heparin 15 µm hemin SuperScript IV VILO Master Mixと8種類の他のマスターミックスについて 記載の濃度での阻害剤添加または無添加の2 µlのrt反応液に1 ngの Total HeLaRNAを用いて比較しました Invitrogen EXPRESS qpcr Supermixを使用し B2M遺伝子をターゲットとするApplied Biosystems TaqMan Assayを加えた1 µlの反応液を用いてqpcrで性能を評価しました 製品名 サイズ SuperScript IV VILO Master Mix SuperScript IV VILO Master Mix with ezdnase Enzyme ezdnase Enzyme PureLink RNA Mini Kit ご注文の際 弊社販売代理店に必ずお伝えください 価格 117565 48,5 この対象製品で 5 反応分 117565 373, この対象製品で 5 反応分 117665 52,5 この対象製品で 5 反応分 117665 383,5 この対象製品で 5 反応分 1176651 2, 1 回分 121832 1,7 5 回分 1218318A 25 回分 キャンペーン番号 MSNA22 製品番号 5 反応分 1218325 プレゼント 32,9 138, 期間 216年1月11日 火 216年12月22日 木 弊社受注分まで 年末キャンペーン対象 関連製品の詳細は 19
11 128! Anza Restriction Enzyme Cloning System 2% OFF Invitrogen Anza Restriction Enzyme Cloning System 1285DNA AnzaAnza 1 DNA 15 Anza AnzaAnza 1 () C: DNA 1: Anza 1 NotI 2: Anza 16 HindIII 3: Anza 15 XmaJI 4: Anza 1 NotI + Anza 16 HindIII + Anza 15 XmaJI L: 1 Kb Plus DNA C 1 2 3 4 L bp 12, 5, 2, 1, 5 3 2 1 1 3 DNA (6,215 bp)invitrogen Anza 1 NotI, Anza 16 HindIII, Anza 15 XmaJI 12 µl1 µgdna1 µl 33 µl1 µg DNA1 µl 37ºC15 Anza 1 NotI 5 units IVGN14 1,7 8,5 2, units IVGN16 42,9 34,3 Anza 11 EcoRI 8, units IVGN116 8,3 6,6 4, units IVGN118 33, 26,4 Anza 12 XbaI 2, units IVGN126 9,9 7,9 12, units IVGN128 4,4 32,3 Anza 16 HindIII 8, units IVGN166 8,3 6,6 4, units IVGN168 33, 26,4 Anza 5 BamHI 8, units IVGN56 8,3 6,6 24, units IVGN58 2,6 16,4 163 www.thermofisher.com/jp-anza REPF2A 216111 2161222 2
PCR 2OFF VeriFlex Applied Biosystems ProFlex PCR ProFlex PCR 332-Well Veriti Veriti 96-Well.2mL SimpliAmp SimpliAmp 1,45,2 1,13,2 72,2 1,16, 9, 576, PCR11 ProFlex PCRVeriti SimpliAmp ProFlex PCR ProFlex PCR, 3 32-Well VeriFlex 1 448473-TCP2 1,45, 1,16, ProFlex PCR, 96-Well VeriFlex 1 448475-TCP2 1,34, 1,7, ProFlex PCR, 2 96-Well 1 448476-TCP2 1,56, 1,24, ProFlex PCR, 2 384-Well 1 448477-TCP2 1,77, 1,4, ProFlex PCR, 2 Flat 1 448478-TCP2 1,77, 1,4, Veriti Veriti 96-Well.2 ml VeriFlex 1 Veriti 2-S2TCP2 1,13, 9, Veriti 96-Well Fast.1 ml VeriFlex 1 Veriti Fast 1-S2TCP2 1,13, 9, Veriti 6-Well.5 ml 1 Veriti 6-S2TCP2 1,13, 9, Veriti 384-Well.2 ml 1 Veriti 384-S2TCP2 1,66, 1,32, SimpliAmp VeriFlex 1 SimpliAmp-S2TCP2 72, 576, 216111 2161222 21
PCR TaqMan Assays Invitrogen SuperScript IV VILO Master Mix 5 A 1 15,8 A TaqMan Fast Advanced Master Mix, 1mL 15,8 TaqMan Gene Expression Master Mix, 1mL 15,8 TaqMan Universal Master Mix II, with UNG, 1mL 15,8 TaqMan Universal Master Mix II, no UNG, 1mL 15,8 1 2 B 1 32,97,9 B 2 81,4141,8 B PureLink RNA Mini Kit, 5 32,9 SuperScript IV VILO Master Mix, 5 48,5 TaqMan Fast Advanced Master Mix, 5 ml 7,9 TaqMan Gene Expression Master Mix, 5 ml 7,9 TaqMan Universal Master Mix II, with UNG, 5 ml 7,9 TaqMan Universal Master Mix II, no UNG, 5 ml 65,2 ID 1ID1 1 TaqMan AssaysS TaqMan Drug Metabolism SNP Genotyping Assays 4362691 TaqMan Mutation Detection Reference Assays 446587 2 TaqMan AssaysM TaqMan Mutation Detection Assays 446584446585 5 TaqMan AssaysL www.thermofisher.com/taqman-point-cp 216111 2161222 22
PCR QuantStudioPCR Applied Biosystems QuantStudio 3QuantStudio 5 PCR PCR Fast13 Fast3 1.5 1 QuantStudio 3 3 QuantStudio 5 6 VeriFlex 384 A B OptiFlex QuantStudio 3 4QuantStudio 56 21 QuantStudio 53845 A B QuantStudio 3 PCR QuantStudio 3 PCR 96 1 QS3-96S-TIP 2 パッケージ 4,7, QuantStudio 3 PCR Fast96 QuantStudio 3 PCR Fast96 1 QS3-96F-TIP 2 4,7, QuantStudio 5 PCR QuantStudio 5 PCR 96 1 QS5-96S-TIP 2 6,87, QuantStudio 5 PCR Fast96 QuantStudio 5 PCR Fast96 1 QS5-96F-TIP 2 6,87, QuantStudio 5 PCR 384 QuantStudio 5 PCR 384 1 QS5-384-TIP 2 7,37, 21659 2161222 23
PCR 216121 1 PCR PureDye Applied Biosystems 7/73/75/75Fast /79/StepOne / StepOnePlus / ViiA 7/QuantStudio 3 / QuantStudio 5 /QuantStudio 6 Flex/ QuantStudio 7 Flex /QuantStudio 12K Flex Kit 79 Well CT TaqMan Amplification Plot 1 ROI Calibration ROI Inspector ROI Calibration Well Fail Background Calibration SYBR Green Amplification Plot 1 ROI Calibration ROI Inspector ViiA7 QuantStudio 6 Flex QuantStudio 7 Flex QuantStudio12K Flex 73 75 75 Fast StepOne StepOnePlus 96-Well Fast 96-Well 384-Well TaqMan Array 96-Well Fast 96-Well 384-Well 96-Well Fast 96-Well 384-Well TaqMan Array 96-Well Fast 96-Well 384-Well TaqMan Array OpenArray 96-Well 96-Well Fast 96-Well A2593 446114 A25931 A25932 A25927 A25928 A25929 A25923 A25924 A25925 A25926 A25913 A25914 A25915 A25916 A25917 449596 449597 449598 446599 4466 35, 35, 35, 45, 35, 35, 35, 35, 35, 35, 45, 35, 35, 35, 45, 61, 29, 29, 29, 17, 17, 1 31, 31, 31, 39, 31, 31, 31, 31, 31, 31, 39, 31, 31, 31, 39, 53, 25, 25, 25, 16, 16, : A26424JP 9, A26425JP 9, A26426JP 9, A26427JP 19, A2646JP 9, A2647JP 9, A2648JP 9, A2649JP 9, A2641JP 9, A26411JP 9, A26412JP 19, A26413JP 9, A2642JP 9, A26421JP 9, A26422JP 19, A26423JP 35, TEL: 12-23-885 216121 217331 24
& Applied Biosystems 35/35xL313/313xl 373/373xl Windows 7 & Data Collection Applied Biosystems 35/35xL313/313xl373/373xl Dell Optiplex XE2Windows 7 : 588,5 19 Windows 7 Data Collection : 535, & Sequencing Analysis v6. : 1,685,3 SeqScape v3. : 1,123,5 Variant Reporter v2. : 56,2 Sequencing Analysis 1,5, Sequencing Analysis v6. : 1,685,3 SeqScape v3. : 1,123,5 Variant Reporter v2. : 56,2 GeneMapper v5. : 1,685,3 Sequencing AnalysisGeneMapper 1,2, 35/35xL & & 1 1 4475183-1 4475183-2 1,5, 1,2, 313 & & 1 1 436969-1 436969-2 1,5, 1,2, 313xl & & 1 1 436968-1 436968-2 1,5, 1,2, 373/373xl & & 1 1 4335644-1 4335644-2 1,5, 1,2, 216111 2161222 25
Invitrogen LentiArray CRISPR CRISPR-Cas9 LentiArray LentiArray 2 grna 4gRNA 2 19 Cas9 sgrna plenti-cas9-p2a-bsd plenticrispr-efs-puro cppt psi PRE P2A WPRE psi cppt PRE sgrna Scaffold Pol lll term WPRE EFS Cas9 Bsd U6 FES Puro LTR LTR LTR Unique sgrna LTR S.pyogenes Cas9 BlasticidinCas92A U6sgRNA EF-1Puromycin Kinase GPCR Epigenetics Phosphatase DNA damage response Cell surface protein Cancer biology Ubiquitin Apoptosis Tumor suppressor Transcription factor Cell cycle Drug transport Ion channel Membrane trafficking Nuclear hormone receptor Protease Druggable genome Whole genome grna 822 446 396 288 516 778 51 943 94 716 1,817 1,444 98 328 141 47 475 1,132 18,392 3,288 1,784 1,584 1,152 2,244 3,112 2,4 3,722 3,616 2,864 7,268 5,776 392 1,312 564 188 1,9 4,512 73,568 A31931 A31947 A31934 A31932 A31946 A31943 A31933 A31935 A3194 A31945 A31938 A31936 A31941 A31942 A31937 A31939 A31944 A31948 A31949 A32167 A32183 A3217 A32168 A32182 A32179 A32169 A32171 A32176 A32181 A32174 A32172 A32177 A32178 A32173 A32175 A3218 A32184 A32185 TEL: 3-6832-93 26
RNAi RNAi sirna Silencer Select sirna 3% OFF Invitrogen Silencer Select sirna LNA sirna212overhang Silencer Select sirna Silencer Select sirna3 OFF 5nM sirna sirna1/11/1sirnaです sirna LNAします Silencer Select Pre-designed sirna5nm Invitrogen Silencer Select Pre-designed sirna 2 7% RNA Silencer Select Pre-designed sirna 32 8%mRNA Silencer Select Validated sirna5nm Invitrogen Silencer Select Validated sirna 8%mRNA Average % mrna remaining 1 9 8 7 6 5 4 3 2 1 8% knockdown D-modified (n = 4) Q-unmodified (n = 3) Silencer Select sirna (n = 3).3nM.3nM 3nM 3nM sirna concentration Silencer Select sirna 1siRNA.33nMsiRNA HeLasiRNA triplicate 48 mrnasirna mrna % 216111 2161222 Pre-designed sirna Validated sirna Custom Designed sirna Custom Select sirna Positive Control Negative Control Silencer Select Pre-designed sirna, inventoried, standard purity Silencer Select Validated sirna, standard purity Silencer Select Custom Designed sirna, standard purity Custom Select sirna, standard purity Silencer Select GAPDH sirna Hs, Mm, Rn Silencer Select Negative Control #1 sirna Silencer Select Negative Control #2 sirna 1 nmol 5 nmol 1 nmol 5 nmol 5 nmol 5 nmol 5 nmol 5 nmol 5 nmol 442737 439242 442738 439824 4399665 439827 439849 439843 439846 13,7 45,2 15,8 57,8 47,3 47,3 31,5 31,5 31,5 9,5 31,6 11, 4,4 33,1 33,1 22, 22, 22, 1 nmol4 Silencer Select sirna ID www.thermofisher.com/taqman-order Email JP.Admin@lifetech.com TEL 3-6832-698 27
48, 69, 2, www.thermofisher.com/antibodies! Invitrogen 3 1. 48, & 2. 7% 3. 2, www.thermofisher.com/antibodies www.thermofisher.com Facebook & Twitter facebook.com/thermofisherjapan @ThermoFisherJP 235959 BioAnalyzer is a trademark of Agilent Technologies, Inc. TaqMan is a registered trademark of Roche Molecular Systems, Inc, used under permission and license. Windows Microsoft Corporation For Research Use Only. Not for use in diagnostic procedures. 216 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 216 1 www.thermofisher.com/jp-tc LSG11-A161OB 18-23 4-2-8 TEL.3-6832-93FAX. 3-6832-958 - - TEL. 12-477-392 TEL. 3-6832-949