2016-2017 INTEGRATED SOLUTIONS page 4 GloMax Discover page 4 GloMax Explorer page 6 GloMax Navigator page 7 GloMax 20/20 page 10 Maxwell RSC page 12 Quantus Fluorometer
S/N Cell based assay NanoLuc GloMax HO N S N S COOH Mg 2+ Luciferase D-Luciferin 生物発光法 Signal 蛍光法 Signal 1,000 100 Caspase-Glo 3/7 Assay Caspase-3/7 Assay BG BG Signal:Noise 10 1 ウェル ( イメージ図 ) ウェル ( イメージ図 ) : 蛍光 / 発光物質 : 化合物 : 生体成分 : 基質 : 励起光 BG : バックグラウンド : 蛍光 / 発光物質 : 化合物 : 生体成分 : 基質生物発光法は試験化合物や生体成分の影響が少ない : 励起光 BG : バックグラウンド 生物発光法は試験化合物や生体成分の影響が少ない 0.1 10 100 1,000 10,000 Number of Cells 5832MC Percent of maximum 120 100 80 60 40 20 Percent of maximum 120 100 80 60 40 20 励起測定フィルターフィルター 0 450 500 550 600 650 700 波長 (nm) 0 450 500 550 600 650 700 波長 (nm) 2
:GloMax Discover : 他社の上位機種検 GloMax GloMax Discover BRET & & 8 NanoLuc 1.E+11 1.E+10 1.E+09 1.E+08 1.E+07 1.E+06 1.E+05 1.E+04 1.E+03 #1 #2 #3 #4 #5 #6 #7 #8 #9 :GloMax Discover : 他社の上位機種 希釈段階 GloMax Instrument GloMax Discover / Explorer GloMax Navigator GloMax Discover / Explorer GloMax Discover / Explorer 10.6 PC PC 1 出器GloMax Discover / Explorer 断面図 検出プレートプレートサンプルトレイカバーオプティカルマスク他社プレートリーダー検出器プレート 100% 100% 0.25% 0.20% 0.15% 0.10% 0.05% 0.00% A1 A2 A3 C1 C2 C3 隣接するウェルへの発光漏れ込み度合 (A2, C2 ウェルが発光している ) 3 器GloMax Navigator 断面図
DISCOVER/EXPLORER GloMax Discover / Explorer System GloMax NanoLuc GloMax Explorer Discover GloMax Discover System UV / BRETFRET GloMax Discover DISCOVER NanoBRET / BRET EXPLORER PC 3 10-21 moles >9 < 3 10-5 6-384 ) & / 1 3 mm NanoBRET / BRET * Discover NanoBRET / BRET * Explorer Discover UV BRET and FRET GloMax Discover GM3000 GloMax Explorer GM3500 GloMax Explorer GM3510 GM3500 GM3520 GM3000 GM3530 GM3000 GM3540 4
GloMax Discover / Explorer 50 NGS BRET FRET ELISA 2 GloMax GloMax Discover / Explorer RealTime-Glo CellTiter-Fluor CellTox Green ONE-Glo ATP CellTiter-Glo 3/7 Caspase-Glo 3/7 / GSH/GSSG-Glo H 2O 2 ROS-Glo Apo-ONE Caspase 3/7 RealTime-Glo ApoLive ND ND ND ND NanoBRET / BRET * Discover GloMax Discover NanoBRET / BRET NanoBRET / BRET BRET YFP Nano NanoLuc HaloTag NanoBRET BRET NanoLuc 蛍光 蛍光標識 HaloTag 96 Corrected mbu 1.71 Z =0.92 0.49 Corrected mbu 1.85 Z =0.71 0.68 384 Protein A Protein B NanoBRET No Drug Nutlin-3 No Drug Nutlin-3 NanoBRET Nutlin-3 p53 Mdm2 GloMax Discover System Z-factor 5
96 Navigator GloMax Navigator System GloMax Navigator 1.5 X 10-21 moles 9 11 GloMax 11 30 PC PC USB ATP CellTiter -Glo 2.0 / CellTiter -Glo CytoTox -Glo ATP BacTiter -Glo RealTime-Glo DNA CellTox Green H 2 O 2 ROS-Glo NADP/ NADPH NAD-Glo GSH / GSSG-Glo Caspase-Glo P450 p450-glo camp camp-glo / Dual-Glo / Dual-Luciferase Assay ONE-Glo Renilla-Glo NanoLuc Nano-Glo / NanoLuc Nano-Glo Dual-Luciferase NanoBiT LgBiT SmBiT Nano-Glo Live Cell Assay 6 30 www.promega.co.jp/product_list.html
20/20 LUMINOMETER GloMax 20/20 Luminometer PMT photon-counting photomultiplier tube S/N 3 10-21 moles 8 1.5 ml PCMicrosoft Excel GloMax 20/20 1.5 ml 35 12 50 GloMax 20/20 18 20 PC 10,000,000 1,000,000 UV Ex 365-395 nm, Em 440-470 nm Hoechst dye 4-methyl-umbelliferone 4-MU RLU 100,000 10,000 1,000 100 10 :GloMax 20/20 : 他社 A : 他社 B Blue Ex 460 nm, Em 515-575 nm EGFP racgfp Fluorescein Protein Quantitation 1 10-19 10e -18 10-17 10-16 10-15 10-14 10-13 10-12 10-11 10-10 ATP 量 (moles) 10-18 moles ATP 8 7
GloMax Discover System GloMax Explorer System 6-384 6-384 BRET UV / BRETFRET [ ]**GM3500 612244896384 47 47 26 cm PC 36 cm 27 kg 100-240 V AC50 / 60 Hz PC Windows 8 USB 100-500 / / +5 45 2 5-200 µl1 µl PMT 3 10-21 moles 9 3 10-5 350-700 nm 5 495 nm SP530 nm LP540 nm SP600 nm LP450 nm8 nm BP * Wavelength-matched LED PIN- UVEx. 365 nm / Em. 415-445 nm BlueEx. 475nm / Em. 500-550 nm GreenEx. 525 nm / Em. 580-640 nm RedEx. 625 nm / Em. 660-720 nm AFCEx. 405 nm / Em. 495-505 nm 2 fmol / 200 µl 6 UV / PMT 200-600 nm 385-600 nm 9 230, 260, 280, 320, 405, 450, 490, 560 600 nm/ 10 nm 5 405, 450, 490, 560,600 nm / 10 nm *GM3500 0-4.0 OD GloMax Discover System / UV / 1 GM3000 4,600,000 GloMax Explorer Fully Loaded Model 1 GM3500 3,900,000 GloMax Dual Injectors with Pumps 1 GM3030 900,000 GloMax Explorer Luminescence and Fluorescence 1 GM3510 3,500,000 GloMax Explorer Absorbance Mod Upgrade GM3510 GM3500 1 GM3520 400,000 GloMax Explorer GM3500 Upgrade to Discover GM3500 GM3000 1 GM3530 900,000 GloMax Explorer GM3510 Upgrade to Discover GM3510 GM3000 1 GM3540 1,300,000 GloMax Dual Injectors with Pumps 1 GM3030 900,000 8
GloMax Navigator System GloMax 20/20 Luminometer Quantus Fluorometer 96 96-47 51 24 cm PC 46 cm 1.5 ml 35 mm 12 mm 50 mm 27 33 21 cm 18 kg 3.65 kg 400 g 0.5 ml PCR 11.5 22.7 4.5cm 0.5A@100-240 V AC50 / 60 Hz 0.5A@100-240 V AC50 / 60 Hz 5 V0.2A Maximum PC Windows 8 USB 2 2 5-200 µl1 µl 25-300 µl5 µl 3 LCD PMT PMT 1.5 10-21 moles 3 10-21 moles > 9 > 8 3 10-5 350-700 nm 350-650 nm Wavelength-matched LED PMT Wavelength-matched LED BlueEx. 460 nm / Em. 515-575 nm UVEx. 365-395 nm / BlueEx. 495 nm / Em. 510-580 nm Em. 440-470 nm RedEx. 640 nm / Em. 660-720 nm 10 ng / ml dsdnahoechst Dye 33258 50 pg/ml dsdnaquantifluor dsdna Dye 5 5 GloMax Navigator System GloMax Navigator System with Dual Injectors and Pump Station + GloMax Navigator Dual Injector and Pump Station Upgrade 1 GM2000 2,000,000 1 GM2010 2,400,000 1 SA1304 400,000 GloMax 20/20 Luminometer GloMax 20/20 Luminometer with Single Injector 1 GloMax 20/20 Luminometer with Dual Injector 2 1 E5311 1,200,000 Quantus Fluorometer 1 E6150 160,000 1 E5321 1,600,000 1 E5331 1,900,000 GloMax 20/20 Fluorescent Module,UV 1 E5351 320,000 GloMax 20/20 Fluorescent Module, BLUE 1 E5361 320,000 DPU-414 1 E2821 160,000 9
& Maxwell RSC Instrument Maxwell DNARNA Maxwell RSC Instrument Quantus 16 33.0 34.5 30.0 cm Quantus Fluorometer UV mirna DNA Quantus Maxwell RSC AS4500 2,800,000 UV Quantus AS3200 300,000 1 16 LEV PCSurface Pro 11 kg 33.0 34.5 30.0 cm 10
Maxwell RSC DNA/ RNA / DNA ( ) ( ) 300 µl / 10 µg 500 µl / 8 µg 250 µl / 50 70 µg 1 10 4 / 50-80 ng 20 mg / 10 µg 200 mg / 50 µg >100 ng/µl DNA Lysis Buffer+ Proeteinase K 5620 Blood DNA Kit Proteinase K Whole Blood DNA Kit 10 10,000 PCR 100 µl Lysis Buffer CTAB 65 30 90 Buffy Coat DNA Kit Cell DNA Purifi cation Kit AS1400 41,000 AS1520 41,000 AS1540 41,000 AS1370 41,000 Plant DNA Kit AS1490 29,000 PureFood GMO and Authentication Kit AS1600 35,000 2 mm 3 / 1 50-500 ng 1-10 5 µm / 2.5 µg DNA Proteinase K Proteinase K RNase Lysis Buffer+ Proeteinase K 70 DNA FFPE Kit DNA FFPE Kit PKK,Custom AS1450 41,000 AX2500 41,000 DNA 10-30 ng / 1 ml 40-120 ng / 4 ml DNA Binding Buffer 200 µl Resin 45 ccfdna Plasma Kit Large Volume ccfdna Plasma Kit, Custom AS1480 96,000 AX1114 120,000 20 mg / 64 µg 1 10 6 / 12 µg 20 mg / 4 µg PCR Homogenization Buffer Lysis Buffer simplyrna Tissue Kit simplyrna Cells Kit AS1340 39,000 AS1390 39,000 Plant RNA Kit AS1500 39,000 RNA 2.5 ml / 6-8 µg Homogenization Buffer Lysis Buffer + Proteinase K 10 simplyrna Blood Kit AS1380 49,000 2 mm 3 / 1 300-700 ng RNA Proteinase K DNase RNA FFPE Kit AS1440 41,000 mirna 20 mg total RNA mirna Homogenization Buffer Lysis Buffer mirna Tissue Kit AS1460 41,000 DNA/ RNA 200 300 µl / RT-qPCR RNA / DNA Lysis Buffer + Proteinase K 5610 Viral Total Nucleic Acid Purifi cation Kit AS1330 51,000 48 11
Quantus Fluorometer QuantiFluor Dye SystemsdsDNAssDNARNA NanoDrop PC Quantus Fluorometer E6150 160,000 11.5 22.7 4.5 cm 400 g BlueEx. 495 nm / Em. 510-580 nm RedEx. 640 nm / Em. 660-720 nm 0.5 ml PCR 5 50 pg/ml dsdnaquantifluor dsdna Dye QuantiFluor Dye 3 LCD 5 USB PC QuantiFluor dsdna Dye 分光光度計 10-3 10-2 10-1 10-0 10 1 10 2 dsdna Concentration (ng/µl) Quantus QuantiFluor dsdna Dye QuantiFluor ONE dsdna System* 500 E4870 29,000 100 E4871 10,000 QuantiFluor dsdna System 1 ml2,000 E2670 52,000 QuantiFluor ssdna System 1 ml2,000 E3190 55,000 QuantiFluor RNA System 1 ml2,000 E3310 59,000 * 1 103-0011 14-15 Tel. 03-3669-7981Fax. 03-3669-7982 532-0011 6-8-8 8704 Tel. 06-6390-7051Fax. 06-6390-7052 Web sitewww.promega.jp 20166 PK1606-04BP