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3 cm S Vimentin Melan A 5 3 HE Canine oral malignant melanoma

4 V ciliated metaplasia 1 Brown, C. C., Baker, D. C. and Baker, I. K Alimentary system. pp In :Pathology of Domestic Animals, 5th ed. Maxie, M. G. ed, Saunders, Philadelphia.

5 X PCV mm Toxoplasma gondii Neospora caninum Sarcocystis cruzi 18S rrna Sarcocystis sp. Sarcocystis sp. Sarcocystis sp. Toxoplasma Neospora Sarcocystis Sarcocystis Sarcocystis

6 mm Gross, T. L., Ihrke, P. J., Walder, E. J. and Affolter, V. K Skin dseases of the dog and cat, clinical and histopathologic diagnosis, 2nd ed, Blackwell Science Ltd, Oxford.

7 EU EU 8,000 NIBS Sus scrofa domestica kg kg 1 NIH NIBS NIBS 6 20 kg kg Fig kg 1988 CSK 3 30 kg6 20 kg NIBS Swine leukocyte antigen, SLA II DRBI D/D DQA D/D DQB SO9 S10/S10 4 Table 1 22

8 8 8 1, 2 E 1 2 SPF NIBS 2 QT 3 NIBS 7 X Fig. 2 NIBS SPF Table 2 NIBS ,300 NIBS Fig. 3 PubMed HI : Haemagglutination inhibition SN: Serum neutralization ELISA:Enzyme linked immunosorbent assay HI HI HI SN SN SN ELISA ELISA

9 ,034,695 9,812,055 8,708,608 8,812,943 6,208, ,677 2,744 15,232 23,682 2,079 3,081 1,829 1,358 1,301 22,646 17,634 17,838 12,759 12,376 1,951 1,584 2,155 2,248 3,462 1 NRC Table 3 4 ECC NRC 7 8 NIBS NS

10 The Laboratory Swine 8 1 Galactose 1.3 galactose GALKO DAF Decay accelerating factor MCP Membrane cofactor protein Table 4 2 GALKO

11 DAF MCP SLA I II SLA SLA NIH SLA NIBS II SPF SPF 3 MRI Magnetic resonance imaging Fig. 4a e NIBS MRI NIBS 1998 OECD Organization for Economic Cooperation and Development TG rd European Congress of Toxicologic Pathology Minipigs in Toxicology 10 EU : : : :

12 : Kano, M., Toyoshi, T., Iwasaki, S., Kato, M., Shimizu, M. and Ota, T QT PRODACT : Usability of miniature pigs in safety pharmacology studies: Assessment for drug induced QT interval prolongation. J. Pharmacol. Sci. 99 : National Research Council Guide for the care and use of laboratory animals. National Academy Press. WA. 5. European Communities Council Directive regarding the protection of animals used for experimental and other scientific procedures. Directive 86/609/EEC National Research Council Nutrient requirements of swine. National Academy Press. WA. 8. Bollen, P. J. A. et al The Laboratory Swine Second Ed.. CRC Press. New York. 9. Somatic cell nuclear transfer Advances in experimental medicine and biology. 591 : Springer. New York. 10. Svendsen, O The minipig in toxicology. Exp. Toxicol. Pathol. 57 : International Pig Veterinary Society Congress;IPVS English Bay Coal Harbour 30

13 Coal Harbour 1 2 Dr. Ernest Stanford Sharing ideas Advancing pig health 3 Welcome reception

14 14 14 International IPVS IPVS 3 Porcine Circo Virus 2 PCV2 Porcine Reproductive and Respiratory Syndrome Virus PRRSV Swine Influenza Virus IPVS 2009 APVS Asian Pig Veterinary Society PCV Dr. C. Fossum Swedish University of Agriculture SciencesPRRSV Dr. J. C. Herring South Dakota State University 1990 Iowa State University Swine Disease Trends US 2000 PCV PCV2 PCV2 2 3 PCV PCV2 3 6

15 PCV2 IgG IgM ELISA IgG IgM 2 PCV2 PCV2 1 PCV2 + M. hyopneumoniae PRRS H1N H1N1 H1N1 H1N1 H1N1 H1N1 USDA H1N a 2012 IPVS Dr. Won Hyung Lee Dr. Ernest Stanford 3b

16 PCV 4 global trend PCV Global Regional

2NIBS CSK 3 20kg NIBS SLA DRBI(D/DDQA(D/D DQBSO9 S10/S10 Table ,2 E 1 2 1,2,5 2,5 15

2NIBS CSK 3 20kg NIBS SLA DRBI(D/DDQA(D/D DQBSO9 S10/S10 Table ,2 E 1 2 1,2,5 2,5 15 食用以外に利用される豚について 齋藤敏樹 ( 日本生物科学研究所 ) Saitoh, T. (2009). Nutlization of a swine other than food ALL about SWINE 35, 14-20 1 2 3 3 12 160 180 kg 12 60 80 kg 12 30 kg NIBS Fig. 1 1995 2007 Table 1 2007 1,300

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