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3 kg 1 Metastrongylus

4 [1 2] [3 6] 1 2 [7] 114 [8] MGH 120 kg 500 kg [ ] 1993 NIBS [22] NIBS 1, 3 GalT KO MGH 30 NIBS GalT KO MGH Koo [23] 6 [24] [25] NIBS GalT KO MGH NIBS 13 5 NIBS A

5 g 6 4 GalT KO MGH 1. B g GalT KO # GalT KO # GalT KO 14 2 GalT KO #1 2 GalT KO #3 GalT KO #4 PCR 2. A 2 GalT KO # B D GalT

6 6 22 SPF GLP [14] NIBS 4.2 [22] 5 7 [25 28] NIBS [7] [14 21] GalT KO MGH GalT KO MGH 0.2 [6] 0.3 [29 31] [32] NIBS g g [33 34] MGH 35 [8 34] 1, 3 [35 36] GalT KO [4 6] GalT KO MGH [37] GalT KO [ ] GalT KO GalT KO 3 NIBS

7 GalT KO MGH SPF GLP Xenotransplantation 2013 ; 20 3 : ONISHI A, IWAMOTO M, AKITA T et al Science 289 : POLEJAEVA IA, CHEN SH, VAUGHT TD et al Nature 407 : ZAIDI A, SCHMOECKEL M, BHATTI F et al Transplantation 65 : DAI Y, VAUGHT TD, BOONE J et al Nat. Biotechnol. 20 : KOLBER SIMONDS D, LAI L, WATT SR et al Proc. Natl. Acad. Sci. USA 101 : LAI L, KOLBER SIMONDS D, PARK K et al Science 295 : LAI L, PRATHER RS Reprod. Biol. Endocrinol. 1 : SACHS DH. MHC Homozygous miniature swine. In : SWIN DLE MM, MOODY DC, PHILLIPS LD, eds. Swine as Models in Biomedical Research. Ames, IA: Iowa State University Press, 1992 :3 15.

8 AHN KS, KIM YJ, KIM M et al Theriogenology 75 : ESTRADA JL, COLLINS B, YORK A et al Cloning Stem Cells 10 : HOSHINO Y, UCHIDA M, SHIMATSU Y et al Cloning Stem Cells 7 : KAWARASAKI T, UCHIYAMA K, HIRAO A et al J Biomed Opt 14 : KOO OJ, PARK HJ, KWON DK et al Zygote 17 : KUROME M, ISHIKAWA T, TOMII R et al J. Reprod. Dev. 54 : LEE SL, KANG EJ, MAENG GH et al J. Reprod. Dev. 56 : LIU HB, LV PR, HE RG et al Cell Reprogram 12 : LI Y, LIU J, DAI J et al Reprod. Domest. Anim. 45 : MIYOSHI K, INOUE S, HIMAKI T et al Mol. Reprod. Dev. 74 : HAO YH, YONG HY, MURPHY CN et al Transgenic Res. 15 : ZHAO J, ROSS JW, HAO Y et al Biol. Reprod. 81 : WAKAI T, SUGIMURA S, YAMANAKA K et al Cloning Stem Cells 10 : NUNOYA T, SHIBUYA K, SAITOH T et al J. Toxicol. Pathol. 20 : KOO OJ, JANG G, KWON DK et al Theriogenology 70 : IM GS, SAMUEL M, LAI L et al Mol. Reprod. Dev. 74 : SHIMATSU Y, UCHIDA M, NIKI R et al Vet. Rec. 155 : BOUCHARD G, MCLAUGHLIN RM, EL- LERSIECK MR et al Lab. Anim. Sci. 45 : CONLEY AJ, JUNG YC, SCHWARTZ NK et al J. Reprod. Fertil. 82 : PANEPINTO LM, PHILLIPS RW, WHEELER LR et al Lab. Anim. Sci. 28 : HILL JR, ROUSSEL AJ, CIBELLI JB et al Theriogenology 51 : KATO Y, TANI T, TSUNODA Y J. Reprod. Fertil. 120 : WELLS DN, MISICA PM, TERVIT HR Biol. Reprod. 60 : WAKAYAMA T, YANAGIMACHI R Nat. Genet. 22 : SACHS DH, SYKES M, ROBSON SC et al Adv. Immunol. 79 : SACHS DH Vet. Immunol. Immunopathol. 43 : GALILI U, RACHMILEWITZ EA, PELEG A et al J. Exp. Med. 160 : GALILI U. Anti alpha galactosyl (anti Gal) antibody damage beyond hyperacute rejection. In: COOPER DKC, KEMP E, PLATT JL, WHITE DJG, eds. Xenotransplantation. Heidelberg: Springer, 1997 : YAMADA K, YAZAWA K, SHIMIZU A et al Nat. Med. 11 : COWAN PJ, CHEN CG, SHINKEL TA et al Transplantation 65 :

9 The 6th Asian Pig Veterinary Society Congress APVS 2013 JASV APVS

10 10 26 PEDV Lawsonia intracellularis Isospora suis Balantidium coli Porcine reproductive and respiratory syndrome PRRS PigRISK Vietnam Salmonella Weltevreden Campylobacter Streptococcus suis MRSA HACCP PRRSV Parma university Paolo Martelli PRRSV INFECTION Immunity and control 1990 PRRS in vivo MA PRRSV in vivo Yuan et al., 1999, Murtaugh et al., 2002 PRRS

11 PRRSV IgM IgG 4 4 IFN 3 NK T PRRSV IFN Diaz et al., 2008 ; Diaz et al., 2012 ; Ferrari et al., 2013; Baumann et al., 2013 PRRS 1 PRRS 2 1 Yes 2 No Zoetis 6 60 PRRS PRRSV PRRSV Stadejek et al., 2005 PRRS Cano et al., 2007 Martelli PRRS ORF T IFN RNA TOHO Genetic characterization and protective immunity of ApxIIA and ApxIIIA of Actinobacillus pleuropneumoniae Characterization of the recently isolated eysipelothrix rhusiopathiae and the effect of commercial erysipelas vaccines 4 5 2

12 12 28 JASV JASV 6 APVS JASV

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